Changing the start temperature and cooling rate in a slow-freezing protocol increases human blastocyst viability

2003 ◽  
Vol 79 (2) ◽  
pp. 407-410 ◽  
Author(s):  
David K Gardner ◽  
Michelle Lane ◽  
John Stevens ◽  
William B Schoolcraft
Keyword(s):  
Cooling Rate ◽  
Slow Freezing ◽  
Human Blastocyst ◽  
Start Temperature

scholarly journals Effect of Cooling Rate on Microstructure and Mechanical Properties in the CGHAZ of Electroslag Welded Pearlitic Rail Steel

Metals ◽  
2019 ◽  
Vol 9 (7) ◽  
pp. 742 ◽  
Author(s):  
Khan ◽  
Yu ◽  
Wang ◽  
Jiang
Keyword(s):  
Mechanical Properties ◽  
Tensile Strength ◽  
Cooling Rate ◽  
Rail Steel ◽  
Interlamellar Spacing ◽  
Coarse Grained ◽  
Microstructure And Mechanical Properties ◽  
Confocal Scanning Laser ◽  
Start Temperature ◽  
Pearlitic Rail Steel

The effect of cooling rate, ranging from 6 to 1 °C/s, on microstructure and mechanical properties in the coarse-grained heat affected zone (CGHAZ) of electroslag welded pearlitic rail steel has been investigated by using confocal scanning laser microcopy (CSLM) and Gleeble 3500 thermo-mechanical simulator. During heating, the formed austenite was inhomogeneous with fractions of untransformed ferrite, which has influenced the pearlite transformation during cooling by providing additional nucleation sites to pearlite. During cooling, at 6 °C/s, the microstructure was composed of martensite and bainite with little pearlite. From 4 to 1 °C/s, microstructures were completely pearlite. Lowering the cooling rate of the CGHAZ from 4 to 1 °C/s increased the pearlite start temperature and reduced the pearlite growth rate. Meanwhile, this increase in pearlite start temperature enlarged the pearlite interlamellar spacing. Alternatively, increasing pearlite interlamellar spacing in the CGHAZ by lowering the cooling rate from 6 to 1 °C/s reduced the hardness and tensile strength, whereas toughness was found unaffected by the pearlite interlamellar spacing. It has been found that a cooling rate of 4 °C/s leads to the formation of pearlite with fine interlamellar spacing of 117 nm in the CGHAZ of electroslag welded pearlitic rail steel where hardness is 425 HV, tensile strength is 1077 MPa, and toughness is 9.1 J.

Outcomes of human blastocyst transfer after slow-freezing using sequential culture: a clinical report

2011 ◽  
Vol 285 (5) ◽  
pp. 1473-1478 ◽  
Author(s):  
Mário Sousa ◽  
Mariana Cunha ◽  
Paulo Viana ◽  
Joaquina Silva ◽  
José Teixeira da Silva ◽  
...  
Keyword(s):  
Slow Freezing ◽  
Blastocyst Transfer ◽  
Clinical Report ◽  
Human Blastocyst

scholarly journals Vitrifying multiple embryos in different arrangements does not alter the cooling rate

2021 ◽  
Author(s):  
Timothy Ostler ◽  
Thomas E Woolley ◽  
Karl Swann ◽  
Andrew Thomson ◽  
Giles Palmer ◽  
...  
Keyword(s):  
Mathematical Model ◽  
Cooling Rate ◽  
Survival Rates ◽  
Spatial Arrangement ◽  
Common Method ◽  
Slow Freezing ◽  
Cooling Rates ◽  
Rapid Cooling ◽  
Rate Of Cooling ◽  
Over Time

Vitrification is the most common method of cryopreservation of gametes in fertility clinics due to its improved survival rates compared to slow freezing techniques. For the Open Cryotop® vitrification device, the number of oocytes, or embryos, mounted onto a single device can vary. In this work, a mathematical model is developed for the cooling of oocytes, or embryos, that is solved computationally, to investigate whether varying the number of samples mounted onto the Open Cryotop® affects the cooling rates, and therefore survival rates, of vitrified samples. Several realistic spatial arrangements of oocytes/embryos are examined, determining the temperature of the system over time, which highlights the effect of spatial arrangement on the rate of cooling. Results indicate that neither spatial arrangement nor the number of mounted oocytes, or embryos, has a large effect on cooling rates, so long as the volume of the cryoprotectant remains minimal. Under the manufacturer's guidelines, clinical decisions regarding the number and arrangement of oocytes or embryos placed on a device can be varied, whilst maintaining rapid cooling.

Effects of cooling rate on the mechanical properties of dual phase treated vanadium steels

1983 ◽  
Vol 41 ◽  
pp. 220-221
Author(s):  
L.J. Chen ◽  
H.C. Cheng ◽  
J.R. Gong ◽  
J.G. Yang
Keyword(s):  
Mechanical Properties ◽  
Cooling Rate ◽  
Automobile Industry ◽  
High Strength ◽  
Weight Percent ◽  
Low Alloy Steels ◽  
Dual Phase ◽  
Resource Requirement ◽  
Alloy Steels ◽  
Potential Weight

For fuel savings as well as energy and resource requirement, high strength low alloy steels (HSLA) are of particular interest to automobile industry because of the potential weight reduction which can be achieved by using thinner section of these steels to carry the same load and thus to improve the fuel mileage. Dual phase treatment has been utilized to obtain superior strength and ductility combinations compared to the HSLA of identical composition. Recently, cooling rate following heat treatment was found to be important to the tensile properties of the dual phase steels. In this paper, we report the results of the investigation of cooling rate on the microstructures and mechanical properties of several vanadium HSLA steels.The steels with composition (in weight percent) listed below were supplied by China Steel Corporation: 1. low V steel (0.11C, 0.65Si, 1.63Mn, 0.015P, 0.008S, 0.084Aℓ, 0.004V), 2. 0.059V steel (0.13C, 0.62S1, 1.59Mn, 0.012P, 0.008S, 0.065Aℓ, 0.059V), 3. 0.10V steel (0.11C, 0.58Si, 1.58Mn, 0.017P, 0.008S, 0.068Aℓ, 0.10V).

Suppression of γ’ Precipitation in a Laser-Melted Nickel-Base Alloy

1977 ◽  
Vol 35 ◽  
pp. 270-271
Author(s):  
J. M. Walsh ◽  
J. C. Whittles ◽  
B. H. Kear ◽  
E. M. Breinan
Keyword(s):  
Cooling Rate ◽  
Base Alloy ◽  
Material Surface ◽  
Intimate Contact ◽  
Absorbed Power ◽  
Perfect Contact ◽  
Nickel Base ◽  
Rapidly Solidified ◽  
Limiting Case ◽  
The Heat Transfer Coefficient

Conventionally cast γ’ precipitation hardened nickel-base superalloys possess well-defined dendritic structures and normally exhibit pronounced segregation. Splat quenched, or rapidly solidified alloys, on the other hand, show little or no evidence for phase decomposition and markedly reduced segregation. In what follows, it is shown that comparable results have been obtained in superalloys processed by the LASERGLAZE™ method.In laser glazing, a sharply focused laser beam is traversed across the material surface at a rate that induces surface localized melting, while avoiding significant surface vaporization. Under these conditions, computations of the average cooling rate can be made with confidence, since intimate contact between the melt and the self-substrate ensures that the heat transfer coefficient is reproducibly constant (h=∞ for perfect contact) in contrast to the variable h characteristic of splat quenching. Results of such computations for pure nickel are presented in Fig. 1, which shows that there is a maximum cooling rate for a given absorbed power density, corresponding to the limiting case in which melt depth approaches zero.

Voids in Quenched Nickel

1968 ◽  
Vol 26 ◽  
pp. 266-267
Author(s):  
J. J. Laidler
Keyword(s):  
Electron Beam ◽  
Ambient Temperature ◽  
Cooling Rate ◽  
Three Dimensional ◽  
Cooling Curve ◽  
Polycrystalline Nickel ◽  
Quenching Temperature ◽  
Long Tail ◽  
Diffusion Pump

The presence of three-dimensional voids in quenched metals has long been suspected, and voids have indeed been observed directly in a number of metals. These include aluminum, platinum, and copper, silver and gold. Attempts at the production of observable quenched-in defects in nickel have been generally unsuccessful, so the present work was initiated in order to establish the conditions under which such defects may be formed.Electron beam zone-melted polycrystalline nickel foils, 99.997% pure, were quenched from 1420°C in an evacuated chamber into a bath containing a silicone diffusion pump fluid . The pressure in the chamber at the quenching temperature was less than 10-5 Torr . With an oil quench such as this, the cooling rate is approximately 5,000°C/second above 400°C; below 400°C, the cooling curve has a long tail. Therefore, the quenched specimens are aged in place for several seconds at a temperature which continuously approaches the ambient temperature of the system.

Microstructures of Laser Processed Fe-Cr Surface Alloys

1981 ◽  
Vol 39 ◽  
pp. 328-329
Author(s):  
P. A. Molian ◽  
K. H. Khan ◽  
W. E. Wood
Keyword(s):  
Cooling Rate ◽  
Pure Iron ◽  
Continuous Wave ◽  
Laser Surface ◽  
Material Surface ◽  
Constitution Diagram ◽  
Incident Power ◽  
Surface Alloying ◽  
Laser Surface Alloying ◽  
Spot Diameter

In recent years, the effects of chromium on the transformation characteristics of pure iron and the structures produced thereby have been extensively studied as a function of cooling rate. In this paper, we present TEM observations made on specimens of Fe-10% Cr and Fe-20% Cr alloys produced through laser surface alloying process with an estimated cooling rate of 8.8 x 104°C/sec. These two chromium levels were selected in order to study their phase transformation characteristics which are dissimilar in the two cases as predicted by the constitution diagram. Pure iron (C<0.01%, Si<0.01%, Mn<0.01%, S=0.003%, P=0.008%) was electrodeposited with chromium to the thicknesses of 40 and 70μm and then vacuum degassed at 400°F to remove the hydrogen formed during electroplating. Laser surface alloying of chromium into the iron substrate was then performed employing a continuous wave CO2 laser operated at an incident power of 1200 watts. The laser beam, defocussed to a spot diameter of 0.25mm, scanned the material surface at a rate of 30mm/sec, (70 ipm).

Preservation of Morphological Integrity and Clot Retraction Activity of Human Platelets After Freezing

1964 ◽  
Vol 11 (01) ◽  
pp. 222-229 ◽  
Author(s):  
Isaac Djerassi ◽  
Albert Roy ◽  
Jorge Alvarado ◽  
Keyword(s):  
Dimethyl Sulfoxide ◽  
Liquid Nitrogen ◽  
Human Platelets ◽  
Slow Freezing ◽  
Critical Conditions ◽  
Plasma Medium ◽  
Clot Retraction ◽  
Controlled Freezing ◽  
Direct Immersion

SummaryHuman platelets frozen at −195° C (liquid nitrogen) retain their morphological integrity and ability to promote clot retraction when 5% dimethyl-sulfoxide and 5% dextrose are added to the suspending plasma medium. Slow freezing was more effective than direct immersion in the liquid nitrogen. Although similar results may be achieved with dimethylsulfoxide alone with rigidly controlled freezing rates, the addition of sugars may permit freezing under less critical conditions.Dimethylsulfoxyd und 5% Dextrose dem Plasmamilieu hinzugefügt werden. Das langsame Einfrieren ist effektiver als das direkte Eintauchen in flüssigen Stickstoff. Obschon ähnliche Resultate mit Dimethylsulfoxyd allein unter exakter Kontrolle der Einfrierungsgeschwindig-keit erreicht werden können, erlaubt die Zugabe von Dextrose ein Einfrieren unter weniger kritischen Bedingungen.

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