258: Evidence for Increased Sensitivity of Bladder Urothelial cell to Carbachol, based on Changes in [CA]I, in Patients with Overactive Bladder (OAB)

AirfugeR (Beckman) direct ultracentrifugation of viral samples on electron microscopy grids offers a rapid way to concentrate viral particles or subunits and facilitate their detection and study. Using the A-100 fixed angle rotor (30°) with a K factor of 19 at maximum speed (95 000 rpm), samples up to 240 μl can be prepared for electron microscopy observation in a few minutes: observation time is decreased and structural details are highlighted. Using latex spheres to calculate the increase in sensitivity compared to the inverted drop procedure, we obtained a 10 to 40 fold increase in sensitivity depending on the size of particles. This technique also permits quantification of viral particles in samples if an aliquot is mixed with latex spheres of known concentration.Direct ultracentrifugation for electron microscopy can be performed on laboratory samples such as gradient or column fractions, infected cell supernatant, or on clinical samples such as urine, tears, cephalo-rachidian liquid, etc..

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Preplate neurons in embryonic mouse cortex: Ultrastructural observations using photoconversion of the fluorescent lipophilic dye dil

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124938 ◽

1992 ◽

Vol 50 (1) ◽

pp. 906-907

Author(s):

Linda C. Hassinger ◽

James E. Crandall

Keyword(s):

Mouse Embryos ◽

Embryonic Mouse ◽

Cortical Afferents ◽

Mouse Cortex ◽

Carbocyanine Dye ◽

Increased Sensitivity

We have begun to look directly at small numbers of afferent axons to early generated neurons that form the preplate in the developing mouse cortex. The carbocyanine dye Dil (1’1, dioctadecyl-3,3,3’3’-tetramethyl-indocarbocyanine) has proved especially useful for this goal. DiI labels axons and their terminals with greater sensitivity and without some of the disadvantages of axon filling with HRP. The increased sensitivity provided by labeling embryonic axons with DiI has given us new insights into the development of cortical afferents. For instance, we reported originally that afferents from the thalamus were present below the cortex as early as embryonic day 15 (E15) based on HRP injections into mouse embryos. By using DiI placements into the thalamus in aldehyde-fixed brains, we now know that thalamic fibers reach the cortex 24 hrs earlier.

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Bayesian removal of noise for increased sensitivity in vector pattern recognition lattice imaging of interfaces

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100150800 ◽

1993 ◽

Vol 51 ◽

pp. 994-995

Author(s):

L. Fei ◽

P. Fraundorf

Keyword(s):

Pattern Recognition ◽

Perfect Crystal ◽

Unit Cell ◽

Ion Milling ◽

Beam Radiation ◽

Major Interest ◽

Unit Cells ◽

Mapping Process ◽

Increased Sensitivity ◽

Electron Microscopes

Interface structure is of major interest in microscopy. With high resolution transmission electron microscopes (TEMs) and scanning probe microscopes, it is possible to reveal structure of interfaces in unit cells, in some cases with atomic resolution. A. Ourmazd et al. proposed quantifying such observations by using vector pattern recognition to map chemical composition changes across the interface in TEM images with unit cell resolution. The sensitivity of the mapping process, however, is limited by the repeatability of unit cell images of perfect crystal, and hence by the amount of delocalized noise, e.g. due to ion milling or beam radiation damage. Bayesian removal of noise, based on statistical inference, can be used to reduce the amount of non-periodic noise in images after acquisition. The basic principle of Bayesian phase-model background subtraction, according to our previous study, is that the optimum (rms error minimizing strategy) Fourier phases of the noise can be obtained provided the amplitudes of the noise is given, while the noise amplitude can often be estimated from the image itself.

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The Effect of Stapedectomy on the Loudness of One’s Own Voice

Journal of Speech and Hearing Research ◽

10.1044/jshr.0803.223 ◽

1965 ◽

Vol 8 (3) ◽

pp. 223-234 ◽

Cited By ~ 1

Author(s):

William Melnick

Keyword(s):

Middle Ear ◽

Normal Subjects ◽

Sound Energy ◽

Loudness Function ◽

Before And After ◽

Increased Sensitivity

Five subjects with normal middle ear mechanisms, and otosclerotic patients, before and after stapedectomy, matched the loudness of their voices to the loudness of a 125-cps-sawtooth noise. The results showed loudness matching functions with gradual slopes, less than 1.00, for the normal subjects and the patients prior to stapedectomy. Post-surgically, the loudness function for the patients increased in steepness to considerably more than 1.00. These results are explained, most logically, in terms of increased sensitivity of the altered middle ear to sound energy generated by the listener’s own voice.

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Modified Earpieces and CROS for High Frequency Hearing Losses

Journal of Speech and Hearing Research ◽

10.1044/jshr.1101.204 ◽

1968 ◽

Vol 11 (1) ◽

pp. 204-218 ◽

Cited By ~ 9

Author(s):

Elizabeth Dodds ◽

Earl Harford

Keyword(s):

Hearing Loss ◽

Hearing Aids ◽

High Frequency ◽

Hearing Aid ◽

Sound Field ◽

Intensity Level ◽

Test Results ◽

High Frequency Hearing Loss ◽

Increased Sensitivity ◽

Difficult Cases

Persons with a high frequency hearing loss are difficult cases for whom to find suitable amplification. We have experienced some success with this problem in our Hearing Clinics using a specially designed earmold with a hearing aid. Thirty-five cases with high frequency hearing losses were selected from our clinical files for analysis of test results using standard, vented, and open earpieces. A statistical analysis of test results revealed that PB scores in sound field, using an average conversational intensity level (70 dB SPL), were enhanced when utilizing any one of the three earmolds. This result was due undoubtedly to increased sensitivity provided by the hearing aid. Only the open earmold used with a CROS hearing aid resulted in a significant improvement in discrimination when compared with the group’s unaided PB score under earphones or when comparing inter-earmold scores. These findings suggest that the inclusion of the open earmold with a CROS aid in the audiologist’s armamentarium should increase his flexibility in selecting hearing aids for persons with a high frequency hearing loss.

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