Secondary Metabolites from Isolated Lichen Mycobionts Cultured Under Different Osmotic Conditions

1995 ◽  
Vol 27 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Nobuo Hamada ◽  
Hisashi Miyagawa
Keyword(s):  
Growth Rate ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Culture Medium ◽  
Culture Conditions ◽  
Metabolite Content ◽  
Conventional Medium ◽  
Gyrophoric Acid

AbstractGrowth of the mycobiont of Ramalina siliquosa and the secondary metabolites subsequently produced under various osmotic culture conditions were examined. The secondary metabolite content and the growth rate changed greatly when different quantities of sucrose were added to the culture medium. Salazinic acid was found only in mycobionts cultured on a medium with 10 or 20% sucrose, the mycobiont growth rate being higher than on conventional medium. Similarly, in Lobaria discolor, gyrophoric acid was found only in mycobionts cultured on a medium with 10% sucrose.

scholarly journals Isolation of sea cucumbers’s (Holothuria atra) secondary metabolite using column-chromatography technique

2021 ◽  
Vol 869 (1) ◽  
pp. 012010
Author(s):  
S Agustina ◽  
S Bella ◽  
S Karina ◽  
I Irwan ◽  
M Ulfah
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Column Chromatography ◽  
Extraction Method ◽  
Sea Cucumber ◽  
Sea Cucumbers ◽  
Marine Chemistry ◽  
Holothuria Atra ◽  
Metabolite Content

Abstract Identification of sea cucumbers from Benteng Inong Balee, Aceh Besar and their phytochemistry screening were conducted in December 2020 to January 2021 at Laboratory of Marine Chemistry and Fisheries Biotechnology, Universitas Syiah Kuala. The purpose of this study was to identify the species of sea cucumbers and its secondary metabolite content using phytochemistry screening and column chromatography. The species of sea cucumbers that were identified was Holothuria atra. The extraction method used in sea cucumber extraction was maceration method, while the separation of secondary metabolites used column-chromatography with eluent of n-hexane : ethyl acetate (8:4). The results showed that secondary metabolites obtained from phytochemical tests were flavonoids, saponins and triterpenoids.

scholarly journals The Growth Comparison of Haematococcus Pluvialis in Two Different Medium

Biota ◽  
2019 ◽  
Vol 12 (2) ◽  
Author(s):  
Dina Soes Putri ◽  
Siti Alaa
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Haematococcus Pluvialis ◽  
Marine Ecosystem ◽  
Culture Conditions ◽  
Vital Role ◽  
Freshwater Microalgae ◽  
Inoculum Concentration ◽  
Chemical Factors ◽  
Physical And Chemical

Microalgae is an aquatic microorganism that conducts photosynthesis. It plays a vital role as an oxygen producer in the marine ecosystem. A freshwater microalgae, Haematococcus pluvialis, has been utilized as a health supplement and industrial application which is beneficial for human. In addition to physical and chemical factors, nutrient composition is one crucial thing that contributes to the growth of microalgae. This present study aimed to determine and compare the growth rate of Haematococcus pluvialis cultivated in two culture medium, Walne’s and Guillard. The culture conditions observed were light intensity, photoperiod of light-dark hours, temperature, inoculum concentration of medium’s liquid, and cell density. This study confirmed that Walne’s media produced much higher biomass (247x104 cells/mL) than Guillard’s medium (209.6x104 cells/mL). The aspect to be further performed on  H. pluvialis biomass is exploring its high-value bio compound. 

scholarly journals Short-Term Root-Zone Temperature Treatment Enhanced the Accumulation of Secondary Metabolites of Hydroponic Coriander (Coriandrum sativum L.) Grown in a Plant Factory

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 413 ◽  
Author(s):  
Duyen T. P. Nguyen ◽  
Na Lu ◽  
Natsuko Kagawa ◽  
Mizuki Kitayama ◽  
Michiko Takagaki
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Root Zone ◽  
Temperature Treatment ◽  
Root Zone Temperature ◽  
Fresh Vegetables ◽  
Short Period ◽  
Plant Factory ◽  
Metabolite Content ◽  
Zone Temperature

The demand for high-nutrient and fresh vegetables, including coriander, has been growing rapidly. A plant factory with artificial lighting enables the application or suppression of stress conditions to plants for producing high-quality vegetables. This study aimed to determine a suitable root-zone temperature (RZT) treatment for enhancing the biomass and secondary metabolite content of hydroponic coriander plants. The combination of a mid-RZT (25 °C) pre-treatment with low (15 °C or 20 °C) or high (30 °C or 35 °C) RZT for a short period (3 or 6 days) was applied to the plants before harvesting. The fresh weights of the coriander plants were reduced under RZT stress. By contrast, the content of secondary metabolites, including ascorbic acid, carotenoids, phenolic compounds, chlorogenic acid, and the antioxidant capacity of the plants were enhanced by the combination of the lowest or highest RZT (15 °C or 35 °C) and the longer stress period (6 days). Growing coriander under an RZT of 30 °C for 6 days can produce large amounts of bioactive compounds and water, whereas growing coriander at an RZT of 15 °C for 6 days can produce high dry biomass and secondary metabolite content.

scholarly journals Secondary Metabolite Production by the Basidiomycete, Lentinus strigellus, under Different Culture Conditions

2012 ◽  
Vol 7 (6) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Bartholomeu A. Barros-Filho ◽  
Maria C. F. de Oliveira ◽  
Jair Mafezoli ◽  
Francisco G. Barbosa ◽  
Edson Rodrigues-Filho
Keyword(s):  
Secondary Metabolites ◽  
Liquid Medium ◽  
Secondary Metabolite ◽  
Culture Media ◽  
Indole Alkaloid ◽  
Culture Conditions ◽  
Secondary Metabolite Production ◽  
Metabolite Production ◽  
Czapek Medium

The basidiomycete Lentinus strigellus was cultivated in three different culture media and the secondary metabolites produced under different culture conditions were isolated and identified. When cultivated in a liquid medium with peptone, L. strigellus afforded the benzopyrans, 2,2-dimethyl-6-methoxychroman-4-one, 4-hydroxy-2,2-dimethyl-6-methoxychromane and (3 R,4 S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. The indole alkaloid echinuline and the anthraquinone fiscione, both unprecedented for the genus Lentinus, were isolated from the mycelium of the fungus. When cultured in Czapek medium enriched with potato broth, the fungus afforded the same benzopyrans except (3 S,4 S)-3,4-dihydroxy-2,2-dimethyl-6-methoxychromane. Panepoxydone and isopanepoxydone were also isolated when the microorganism was grown in Czapek medium.

scholarly journals Variation in Resistance of Mycobacterium paratuberculosis to Acid Environments as a Function of Culture Medium

2003 ◽  
Vol 69 (11) ◽  
pp. 6833-6840 ◽  
Author(s):  
Nackmoon Sung ◽  
Michael T. Collins
Keyword(s):  
Growth Rate ◽  
Culture Medium ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Acid Resistance ◽  
Culture Conditions ◽  
Mycobacterium Paratuberculosis ◽  
In Vitro Susceptibility ◽  
Sds Page

ABSTRACT Acid resistance of Mycobacterium paratuberculosis was examined as a function of growth conditions (i.e., in vitro growth medium and pH). M. paratuberculosis was cultured in either fatty acid-containing medium (7H9-OADC) or glycerol-containing medium (WR-GD or 7H9-GD) at two culture pHs (pHs 6.0 and 6.8). Organisms produced in these six medium and pH conditions were then tested for resistance to acetate buffer at pHs 3, 4, 5, and 6 at 20°C. A radiometric culture method (BACTEC) was used to quantify viable M. paratuberculosis cell data at various acid exposure times, and D values (decimal reduction times, or the times required to kill a 1-log10 concentration of bacteria) were determined. Soluble proteins of M. paratuberculosis grown under all six conditions were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) to identify proteins that may be associated with acid resistance or susceptibility. The culture medium affected growth rate and morphology: thin floating sheets of cells were observed in 7H9-OADC versus confluent, thick, waxy, and wrinkled pellicles in WR-GD. Culture medium pH affected growth rate (which was highest at pH 6.0), but it had little or no effect on D values for M. paratuberculosis at any test pH. When grown in 7H9-OADC, M. paratuberculosis was more acid resistant at all test pHs (higher D values) than when grown in WR-GD. Glycerol appeared to be the culture medium component most responsible for lower levels of M. paratuberculosis acid resistance. When glycerol was substituted for OADC in the 7H9 medium, D values were significantly lower than those of 7H9-OADC-grown M. paratuberculosis and were approximately the same as those for M. paratuberculosis grown in WR-GD medium. Comparison of the SDS-PAGE protein profiles for M. paratuberculosis cultures grown in 7H9-OADC, WR-GD, or 7H9-GD medium revealed that increased expression of 34.2- and 14.0-kDa proteins was associated with higher levels of acid resistance of M. paratuberculosis grown in 7H9-OADC medium and that 56.6- and 41.3-kDa proteins were associated with lower levels of acid resistance. This is the first report showing that in vitro culture conditions significantly affect growth characteristics, acid resistance, and protein expression of M. paratuberculosis, and the results emphasize the importance of culture conditions for in vitro susceptibility studies.

scholarly journals The Influence of Seasonality on Secondary Metabolite Profiles and Neuroprotective Activities of Moss Hypnum cupressiforme Extracts: In Vitro and In Silico Study

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 123
Author(s):  
Tanja M. Lunić ◽  
Marija R. Mandić ◽  
Mariana M. Oalđe Pavlović ◽  
Aneta D. Sabovljević ◽  
Marko S. Sabovljević ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Biological Activities ◽  
Hypnum Cupressiforme ◽  
Tnf Α ◽  
In Silico Study ◽  
Metabolite Profiles ◽  
Metabolite Content

Numerous representatives of mosses, including Hypnum cupressiforme, have been used to alleviate different inflammation-related conditions. However, the mode of action underlying this anti-inflammatory potential has been poorly understood. Moreover, the influence of seasonality on the chemical composition and biological activity of mosses is generally overlooked. This study aimed to investigate the influence of seasonal changes (spring, summer, and autumn) on secondary metabolite composition and biological activities of ethyl acetate H. cupressiforme extracts. Antioxidant activity was measured using β-carotene bleaching assay, while MTT, NBT, ELISA, and Griess assays were carried out to explore the anti-neuroinflammatory and neuroprotective potential of extracts. Inhibitory activities on acetylcholinesterase and tyrosinase were assessed experimentally and by docking analysis. The highest content of secondary metabolites and antioxidant activity were observed in moss during the summer. Extracts inhibited the secretion of ROS, NO, TNF-α, and IL-6, alleviating the inflammatory potential of H2O2 and LPS in microglial and neuronal cells. Strong inhibitory effects on acetylcholinesterase and tyrosinase were observed in vitro. Docking analyses revealed high-affinity interactions of secondary metabolites present in H. cupressiforme with important enzyme residues. Altogether, these results reveal the neuroprotective potential and the significance of seasonal fluctuations on secondary metabolite content and biological activities in moss H. cupressiforme.

scholarly journals Comparison of Secondary Metabolite Content of Pteris vittata L. in Baluran National Park and Malang and Its Effect on Environment

el–Hayah ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 1-5
Author(s):  
Eko Sri Sulasmi ◽  
Uun Rohmawati ◽  
Achmad Makin Amin
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
National Park ◽  
Pteris Vittata ◽  
Screening Methods ◽  
State University ◽  
Phytochemical Screening ◽  
Qualitative Test ◽  
The Difference ◽  
Metabolite Content

Fern is cosmopolitan plants which are almost scattered in all parts of the world, one of which is found in Baluran National Park and in Malang. The potential and benefits of these ferns are quite important for agriculture and medicine because of the chemical compounds they have, especially in Pteris vitatta L. This study aimed to analyze secondary metabolites contained in P. vittata L. in Baluran National Park, Situbondo and in Malang. The samples used were leaves and rhizome P. vittata extracted using methanol 96%, followed by a qualitative test of the content of alkaloids, flavonoids, terpenoids, polyphenols, tannins, saponins, using phytochemical screening methods with several reagents. The results showed that the leaves and Rhizome P. vittata L.. in Baluran National Park, Situbondo were positively containing secondary metabolites of terpenoids, polyphenols, tannins, saponins, and alkaloids (Dragendorf and Bouchardat reagents), whereas flavonoids were not present in all samples. However, the results of P. vitatta L. phytochemical screening around Malang State University positively contained flavonoids, polyphenols, terpenoids, alkaloids (Wagner and Dragendorf reagents). The difference in results from these two places is because the secondary metabolite content in plants is affected by stressful environmental conditions such as soil texture where it is grown or is affected by the precursors of the secondary metabolites of the metabolites.

scholarly journals Discovery of a polybrominated aromatic secondary metabolite from a planctomycete points at an ambivalent interaction with its macroalgae host

2019 ◽  
Author(s):  
Fabian Panter ◽  
Ronald Garcia ◽  
Angela Thewes ◽  
Nestor Zaburannyi ◽  
Boyke Bunk ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Biological Activities ◽  
Genome Mining ◽  
Gene Clusters ◽  
Laboratory Culture ◽  
Culture Conditions ◽  
Herbicidal Activity ◽  
Spectrometric Analysis ◽  
Plant Toxin

AbstractThe roles of the majority of bacterial secondary metabolites, especially those from uncommon sources are yet elusive even though many of these compounds show striking biological activities. To further investigate the secondary metabolite repertoire of underexploited bacterial families, we chose to analyze a novel representative of the yet untapped bacterial phylum Planctomycetes for the production of secondary metabolites under laboratory culture conditions. Development of a planctomycetal high density cultivation technique in combination with high resolution mass spectrometric analysis revealed Planctomycetales strain 10988 to produce the plant toxin 3,5 dibromo p-anisic acid. This molecule represents the first secondary metabolite reported from any planctomycete. Genome mining revealed the biosynthetic origin of this doubly brominated secondary metabolite and a biosynthesis model for the com-pound was devised. Comparison of the biosynthetic route to biosynthetic gene clusters responsible for formation of polybrominated small aromatic compounds reveals evidence for an evolutionary link, while the compound’s herbicidal activity points towards an ambivalent role of the metabolite in the planctomycetal ecosystem.

scholarly journals POTENSI BEBERAPA JENIS RUMPUT LAUT DI ACEH (STUDI KASUS: SKRINING FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN)

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Adean Mayasri
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Seaweed Species ◽  
Dpph Method ◽  
Metabolite Content

One of the sea potentials in Aceh is seaweed. Seaweed contains secondary metabolites and antioxidants that are very well consumed because it can act as an antidote/protector from the free radicals. This study aimed to determine the secondary metabolite content and antioxidant activity of seaweed in Aceh.The seaweed that has been macerated then screened for phytochemicals and tested for antioxidant activity by using the DPPH method. The seaweed species found were Gracillaria verrucosa, Sargassum sp., and Chaetomorpha antennina. The results of this study indicated that Gracillaria verrucosa, Sargassum sp., and Chaetomorpha antennina seaweeds were contained by alkaloids and steroids. Flavonoid and saponins were only detected in the types of seaweed Gracillaria verrucosa and Sargassum sp. It was also found that the higher the concentration of the extract, the higher the percentage of IC50 against free radicals. The Sargassum sp. has a stable percentage of inhibition at a 2-10 ppm concentration, so it is recommended in this study to be considered cultivated in Aceh.

scholarly journals ANALISIS KUALITATIF KANDUNGAN BUNGA KENANGA (Cananga odorata) SECARA FITOKIMIA dengan MENGGUNAKAN PELARUT ETANOL

2020 ◽  
Vol 2 (1) ◽  
pp. 43
Author(s):  
Putri Ade Rahma Yulis ◽  
Aisyah meiysa putri ◽  
Anasthasia oca muham ◽  
Silvia anggraini ◽  
Siti maisarmah
Keyword(s):  
Secondary Metabolites ◽  
Essential Oils ◽  
Secondary Metabolite ◽  
Active Metabolite ◽  
Antibacterial Properties ◽  
Raw Material ◽  
Research Results ◽  
Qualitative Test ◽  
Metabolite Content ◽  
Cananga Odorata

Cananga (Cananga odorata) is a tree or shrub that can be used to produce essential oils. In this study a qualitative test was conducted to study the content of secondary metabolites in cananga flowers. Based on research results cananga flowers contain flavonoids, tannins, saponins and steroids, while anthraquinone and terpenoids are not needed. The existence of secondary metabolite content in this plant is intended to be used as a raw material needed because it has an active metabolite content that links this plant to have antioxidant and other antibacterial properties.

Sign in / Sign up

Export Citation Format

Share Document