scholarly journals Comparison of Secondary Metabolite Content of Pteris vittata L. in Baluran National Park and Malang and Its Effect on Environment

el–Hayah ◽  
2019 ◽  
Vol 7 (1) ◽  
pp. 1-5
Author(s):  
Eko Sri Sulasmi ◽  
Uun Rohmawati ◽  
Achmad Makin Amin
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
National Park ◽  
Pteris Vittata ◽  
Screening Methods ◽  
State University ◽  
Phytochemical Screening ◽  
Qualitative Test ◽  
The Difference ◽  
Metabolite Content

Fern is cosmopolitan plants which are almost scattered in all parts of the world, one of which is found in Baluran National Park and in Malang. The potential and benefits of these ferns are quite important for agriculture and medicine because of the chemical compounds they have, especially in Pteris vitatta L. This study aimed to analyze secondary metabolites contained in P. vittata L. in Baluran National Park, Situbondo and in Malang. The samples used were leaves and rhizome P. vittata extracted using methanol 96%, followed by a qualitative test of the content of alkaloids, flavonoids, terpenoids, polyphenols, tannins, saponins, using phytochemical screening methods with several reagents. The results showed that the leaves and Rhizome P. vittata L.. in Baluran National Park, Situbondo were positively containing secondary metabolites of terpenoids, polyphenols, tannins, saponins, and alkaloids (Dragendorf and Bouchardat reagents), whereas flavonoids were not present in all samples. However, the results of P. vitatta L. phytochemical screening around Malang State University positively contained flavonoids, polyphenols, terpenoids, alkaloids (Wagner and Dragendorf reagents). The difference in results from these two places is because the secondary metabolite content in plants is affected by stressful environmental conditions such as soil texture where it is grown or is affected by the precursors of the secondary metabolites of the metabolites.

scholarly journals ANALISIS KUALITATIF KANDUNGAN BUNGA KENANGA (Cananga odorata) SECARA FITOKIMIA dengan MENGGUNAKAN PELARUT ETANOL

2020 ◽  
Vol 2 (1) ◽  
pp. 43
Author(s):  
Putri Ade Rahma Yulis ◽  
Aisyah meiysa putri ◽  
Anasthasia oca muham ◽  
Silvia anggraini ◽  
Siti maisarmah
Keyword(s):  
Secondary Metabolites ◽  
Essential Oils ◽  
Secondary Metabolite ◽  
Active Metabolite ◽  
Antibacterial Properties ◽  
Raw Material ◽  
Research Results ◽  
Qualitative Test ◽  
Metabolite Content ◽  
Cananga Odorata

Cananga (Cananga odorata) is a tree or shrub that can be used to produce essential oils. In this study a qualitative test was conducted to study the content of secondary metabolites in cananga flowers. Based on research results cananga flowers contain flavonoids, tannins, saponins and steroids, while anthraquinone and terpenoids are not needed. The existence of secondary metabolite content in this plant is intended to be used as a raw material needed because it has an active metabolite content that links this plant to have antioxidant and other antibacterial properties.

scholarly journals Isolation of sea cucumbers’s (Holothuria atra) secondary metabolite using column-chromatography technique

2021 ◽  
Vol 869 (1) ◽  
pp. 012010
Author(s):  
S Agustina ◽  
S Bella ◽  
S Karina ◽  
I Irwan ◽  
M Ulfah
Keyword(s):  
Secondary Metabolites ◽  
Ethyl Acetate ◽  
Secondary Metabolite ◽  
Column Chromatography ◽  
Extraction Method ◽  
Sea Cucumber ◽  
Sea Cucumbers ◽  
Marine Chemistry ◽  
Holothuria Atra ◽  
Metabolite Content

Abstract Identification of sea cucumbers from Benteng Inong Balee, Aceh Besar and their phytochemistry screening were conducted in December 2020 to January 2021 at Laboratory of Marine Chemistry and Fisheries Biotechnology, Universitas Syiah Kuala. The purpose of this study was to identify the species of sea cucumbers and its secondary metabolite content using phytochemistry screening and column chromatography. The species of sea cucumbers that were identified was Holothuria atra. The extraction method used in sea cucumber extraction was maceration method, while the separation of secondary metabolites used column-chromatography with eluent of n-hexane : ethyl acetate (8:4). The results showed that secondary metabolites obtained from phytochemical tests were flavonoids, saponins and triterpenoids.

scholarly journals A Preliminary Screening of the Different of Secondary Metabolites Ruku-Ruku Leaves (Ocimum tenuiflorum Linnen) in West Sumatera

2019 ◽  
Vol 20 (2) ◽  
pp. 17-24
Author(s):  
Hesty Parbuntari ◽  
Sri Benti Etika ◽  
Melindra Mulia ◽  
Elfanny Delvia
Keyword(s):  
Secondary Metabolites ◽  
Medicinal Plant ◽  
Essential Oils ◽  
Bioactive Compounds ◽  
Light Exposure ◽  
Phytochemical Screening ◽  
Preliminary Screening ◽  
West Sumatra ◽  
Ocimum Tenuiflorum ◽  
Qualitative Test

Ruku-ruku leaves are used to remove distinctive odors from fish in some cuisine of West Sumatra. The characteristic odor comes from secondary metabolites especially essential oils. Therefore, secondary metabolites in ruku-ruku leaves have the potential to be further investigated as a source of bioactive compounds. The phytochemical screening of ruku-ruku leaves is a preminilary qualitative test that needs to be done to maximise its function as a medicinal plant. Based on the result data, there are some different result of flavonoid, steroids, and triterpenoids identification. Ruku-ruku leaves from Pesisir Selatan and Padang Panjang shows less flavonoid compounds tham Payakumbuh, Batusangkar, and Lubuk Alung. Both of this city and Payakumbuh also gives less steroids and triterpenoids. The factor influencing this result is caused by several factors, namely temperature, humidity, light exposure, and salinity.

scholarly journals Short-Term Root-Zone Temperature Treatment Enhanced the Accumulation of Secondary Metabolites of Hydroponic Coriander (Coriandrum sativum L.) Grown in a Plant Factory

Agronomy ◽  
2020 ◽  
Vol 10 (3) ◽  
pp. 413 ◽  
Author(s):  
Duyen T. P. Nguyen ◽  
Na Lu ◽  
Natsuko Kagawa ◽  
Mizuki Kitayama ◽  
Michiko Takagaki
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Root Zone ◽  
Temperature Treatment ◽  
Root Zone Temperature ◽  
Fresh Vegetables ◽  
Short Period ◽  
Plant Factory ◽  
Metabolite Content ◽  
Zone Temperature

The demand for high-nutrient and fresh vegetables, including coriander, has been growing rapidly. A plant factory with artificial lighting enables the application or suppression of stress conditions to plants for producing high-quality vegetables. This study aimed to determine a suitable root-zone temperature (RZT) treatment for enhancing the biomass and secondary metabolite content of hydroponic coriander plants. The combination of a mid-RZT (25 °C) pre-treatment with low (15 °C or 20 °C) or high (30 °C or 35 °C) RZT for a short period (3 or 6 days) was applied to the plants before harvesting. The fresh weights of the coriander plants were reduced under RZT stress. By contrast, the content of secondary metabolites, including ascorbic acid, carotenoids, phenolic compounds, chlorogenic acid, and the antioxidant capacity of the plants were enhanced by the combination of the lowest or highest RZT (15 °C or 35 °C) and the longer stress period (6 days). Growing coriander under an RZT of 30 °C for 6 days can produce large amounts of bioactive compounds and water, whereas growing coriander at an RZT of 15 °C for 6 days can produce high dry biomass and secondary metabolite content.

scholarly journals PHYTOCHEMICAL SCREENING OF ETHANOLIC EXTRACT: a Preliminary Test on Five Medicinal Plants on Bangkalan

2020 ◽  
Vol 7 (2) ◽  
pp. 96-102
Author(s):  
Laila Khamsatul Muharrami ◽  
Fatimatul Munawaroh ◽  
Taslim Ersam ◽  
Mardi Santoso
Keyword(s):  
Medicinal Plants ◽  
Secondary Metabolite ◽  
Screening Test ◽  
Ethanolic Extract ◽  
Preliminary Test ◽  
Punica Granatum ◽  
Medicinal Herb ◽  
Phytochemical Screening ◽  
Phytochemical Content ◽  
Metabolite Content

This study is preliminary research of secondary metabolite potential from medicinal Herb Madura. This study aims to know the medicinal plants used in the medicinal herb Madura and the phytochemical content contained in these medicinal plants.  The method used in this study is a survey to know the kinds of medicinal plants used in Madura Herb. Then a phytochemical screening test for the medicinal plant was performed. The phytochemical screening test used are alkaloids, steroids, saponins, and flavonoids.  Based on the research, it is found that 66 medicinal plants are widely used for "Ramuan Madura” in  Bangkalan. Of the 66 plants selected, Madura five medicinal plants widely used by the Madurense; Parameria laevigata, Kaempferia galanga L., Curcuma domestica, Curcuma xanthorrhiza, dan Punica granatum. Then the preliminary phytochemical test to determine the secondary metabolite compounds. Secondary metabolite content obtained include alkaloids, saponins, steroids, and flavonoids.

scholarly journals Biolarvicidal activity of Rhizophora stylosa leaf extract against Aedes sp.

2021 ◽  
Vol 869 (1) ◽  
pp. 012062
Author(s):  
D Syahputra ◽  
S Karina ◽  
Irwan ◽  
S Agustina ◽  
M Ulfah
Keyword(s):  
Secondary Metabolites ◽  
Leaf Extract ◽  
Methanol Extract ◽  
Probit Analysis ◽  
Phytochemical Screening ◽  
Research Focus ◽  
Rhizophora Stylosa ◽  
Marine Chemistry ◽  
Qualitative Test ◽  
Banda Aceh

Abstract The research focus on biolarvicidal activity of Rhizophora Stylosa leaf extract against Aedes sp. R. stylosa leaf was collected from Gampong Pande, Banda Aceh. This research was conducted at laboratory of Marine Chemistry and Fisheries Biotechnology, Marine and Fisheries Faculty, Universitas Syiah Kuala from Desember 2020 to March 2021. The objective of this study was to figure out the secondary metabolites content in the methanol extract of the mangrove leaf and its biolarvicidal activity against Aedes sp. larvae. The secondary metabolites were screened out using phytochemical screening. The extract was tested for its biolarvicidal activity against third instar larvae of the Aedes sp. mosquito at incubation’s time of 48 hours. The biolarvicidal activity is expressed in LC50 which was determined by probit analysis using SPSS. The results of qualitative test showed that the extract contained of flavonoids, saponins, triterpenoids and tannins. Based on the results of probit analysis, the LC50 value of the extract against Aedes sp. was 858.89 ppm.

scholarly journals Identification of Active Compounds on Muntingia calabura L.Leaves using Different Polarity Solvents

2020 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Sri Adelila Sari ◽  
Mellya Ernita ◽  
M Nasir Mara ◽  
Muhammad Rudi AR
Keyword(s):  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Screening Tests ◽  
Phytochemical Screening ◽  
Polar Solvents ◽  
The Public ◽  
Dark Green ◽  
Selection Stage ◽  
Ethanol Series ◽  
Muntingia Calabura

Plant of Muntingia calabura L are often known as “kersen”, "seri or "cherry". Leaves of Muntingia calabura L. contains many benefits but its properties are still little known to the public. It contains secondary metabolites which have many uses. This study was aimed to determine the content of secondary metabolites in this leaf. Leaves extracts were obtained by maceration extraction for 3 times 24 hours using polar, semi-polar and non-polar solvents to determine the solubility of secondary metabolite compounds in each solvent. The solvents used were ethanol, ethyl acetate and n-hexane. The leaveswere dissolved a lot in polar solvents, marked by the formation of a dark green color in ethanol-series extracts, the color fades more in semi-polar and non-polar solvents. The three leaves extracts were tested for secondary metabolite contents by phytochemical screening tests. Phytochemical screening was an initial selection stage to detect classes of chemical compounds contained in plant. Phytochemical screening were included alkaloid, terpenoid, steroid, tannin, flavonoidand saponin tests. Based on the results of phytochemical screening tests, the leaf was contained several secondary metabolite compounds, namely flavonoids, saponins, steroids, terpenoids, alkaloids, phenols and tannins.

scholarly journals The Influence of Seasonality on Secondary Metabolite Profiles and Neuroprotective Activities of Moss Hypnum cupressiforme Extracts: In Vitro and In Silico Study

Plants ◽  
2022 ◽  
Vol 11 (1) ◽  
pp. 123
Author(s):  
Tanja M. Lunić ◽  
Marija R. Mandić ◽  
Mariana M. Oalđe Pavlović ◽  
Aneta D. Sabovljević ◽  
Marko S. Sabovljević ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Biological Activities ◽  
Hypnum Cupressiforme ◽  
Tnf Α ◽  
In Silico Study ◽  
Metabolite Profiles ◽  
Metabolite Content

Numerous representatives of mosses, including Hypnum cupressiforme, have been used to alleviate different inflammation-related conditions. However, the mode of action underlying this anti-inflammatory potential has been poorly understood. Moreover, the influence of seasonality on the chemical composition and biological activity of mosses is generally overlooked. This study aimed to investigate the influence of seasonal changes (spring, summer, and autumn) on secondary metabolite composition and biological activities of ethyl acetate H. cupressiforme extracts. Antioxidant activity was measured using β-carotene bleaching assay, while MTT, NBT, ELISA, and Griess assays were carried out to explore the anti-neuroinflammatory and neuroprotective potential of extracts. Inhibitory activities on acetylcholinesterase and tyrosinase were assessed experimentally and by docking analysis. The highest content of secondary metabolites and antioxidant activity were observed in moss during the summer. Extracts inhibited the secretion of ROS, NO, TNF-α, and IL-6, alleviating the inflammatory potential of H2O2 and LPS in microglial and neuronal cells. Strong inhibitory effects on acetylcholinesterase and tyrosinase were observed in vitro. Docking analyses revealed high-affinity interactions of secondary metabolites present in H. cupressiforme with important enzyme residues. Altogether, these results reveal the neuroprotective potential and the significance of seasonal fluctuations on secondary metabolite content and biological activities in moss H. cupressiforme.

scholarly journals Skrining Fitokimia Senyawa Metabolit Sekunder Terhadap Ekstrak Tanaman Ranting Patah Tulang (Euphorbia tirucalli L.)

2020 ◽  
Vol 1 (1) ◽  
pp. 24
Author(s):  
Abdul Rahman Wahid ◽  
Safwan Safwan
Keyword(s):  
Antioxidant Activity ◽  
Data Analysis ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Extraction Process ◽  
Phytochemical Screening ◽  
Euphorbia Tirucalli ◽  
Analysis Technique ◽  
The Family ◽  
Garden Plant

ABSTRAKTanaman patah tulang termasuk dalam famili Euphorbiaceae, merupakan jenis tanaman kebun yang tumbuh tegak hingga setinggi 2-6m. Kandungan senyawa metabolit sekunder tanaman Euphorbia tirucalli L yaitu flavonid, fenol, saponin, dan tanin Penelitian ini dilakukan untuk mengetahui kandungan senyawa metabolit sekunder dan aktivitas antioksidan daun ranting tanaman patah tulang. Senyawa metabolit sekunder diperoleh dari proses ekstraksi dengan pelarut etanol menggunakan metode maserasi dan partisi. Metode penelitian skrining fitokimia dilakukan dengan uji warna menggunakan berbagai pereaksi. Teknik analisis data dilakukan secara kualitatif. Hasil penelitian pada skrining fitokimia diperoleh positif mengandung flavonoid yang ditandai dengan adanya perubahan warna menjadi kuning, tanin dan steroid sedangkan alkaloid, saponin dan triterpenoid negatif. Penelitian ini dapat disimpulkan bahwa tanaman patah tulang mengandung senyawa metabolit sekunder yaitu flavonoid, tanin dan steroid. Kata kunci : Pengetahuan; Kepatuhan; Tuberkulosis. ABSTRACTBroken bones plants belong to the family Euphorbiaceae, a type of garden plant that grows up to 2-6m tall. The content of secondary metabolites of Euphorbia tirucalli L plants namely flavonids, phenols, saponins, and tannins This study was conducted to determine the content of secondary metabolite compounds and the antioxidant activity of twig leaves of fractured plants. Secondary metabolites are obtained from the extraction process with ethanol solvent using maceration and partitioning methods. Phytochemical screening research methods are carried out by color testing using various reagents. The data analysis technique was carried out qualitatively. The results of the study on phytochemical screening showed that they positively contained flavonoids which were marked by changes in color to yellow, tannins and steroids while the alkaloids, saponins and triterpenoids were negative. This study can be concluded that fracture plants contain secondary metabolite compounds namely flavonoids, tannins and steroids. Keywords : Knowledge; Compliance; Tuberculosis.

scholarly journals POTENSI BEBERAPA JENIS RUMPUT LAUT DI ACEH (STUDI KASUS: SKRINING FITOKIMIA DAN AKTIVITAS ANTIOKSIDAN)

2021 ◽  
Vol 9 (1) ◽  
Author(s):  
Adean Mayasri
Keyword(s):  
Antioxidant Activity ◽  
Free Radicals ◽  
Secondary Metabolites ◽  
Secondary Metabolite ◽  
Seaweed Species ◽  
Dpph Method ◽  
Metabolite Content

One of the sea potentials in Aceh is seaweed. Seaweed contains secondary metabolites and antioxidants that are very well consumed because it can act as an antidote/protector from the free radicals. This study aimed to determine the secondary metabolite content and antioxidant activity of seaweed in Aceh.The seaweed that has been macerated then screened for phytochemicals and tested for antioxidant activity by using the DPPH method. The seaweed species found were Gracillaria verrucosa, Sargassum sp., and Chaetomorpha antennina. The results of this study indicated that Gracillaria verrucosa, Sargassum sp., and Chaetomorpha antennina seaweeds were contained by alkaloids and steroids. Flavonoid and saponins were only detected in the types of seaweed Gracillaria verrucosa and Sargassum sp. It was also found that the higher the concentration of the extract, the higher the percentage of IC50 against free radicals. The Sargassum sp. has a stable percentage of inhibition at a 2-10 ppm concentration, so it is recommended in this study to be considered cultivated in Aceh.

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