Collection frequency affects percent Y-chromosome bearing sperm, sperm head area and quality of bovine ejaculates

2002 ◽  
Vol 57 (4) ◽  
pp. 1327-1346 ◽  
Author(s):  
John E Chandler ◽  
Anita M Canal ◽  
J.B Paul ◽  
E.B Moser
Keyword(s):  
2020 ◽  
Vol 7 (15) ◽  
pp. 191-209
Author(s):  
Christian Chima Chukwu ◽  
Ignatius Sunday Ume

Celebration of childbirth, among the Igbo, is looked upon as an occasion of feasting and so every festal feature, especially good music is made available as a mark of thanksgiving to God. The birth of a new child is announced with a special kind of song called irri muo, and it is sung in celebration of the birth. Songs sung at childbirth are called umanwa, while in neighbouring communities, they are egwu omugho. Umanwa music is exclusively performed by women, and has survived until today in its original form through oral tradition. This paper, therefore, examines the celebration of childbirth through dance and the demystification of the male child in the Igbo Patriarchal Society. Among the findings, the paper acknowledges that there is always some excitement, merriment, joy and intoxication, mixed with some sparks of faith when the new entrant is added to a household. In addition, the paper recognizes that women are never fully recognized as mothers until the birth of a boy child. Disturbing as the preference is, this paper wonders and questions why women are being easily ridiculed, subjugated and divorced when and where the Y-chromosome, the sole determinant of the birth of the boy child is the exclusive preserve of the male gender. With this, the paper argues that since human personality, a prerogative and quality of every human being does not rest on gender, but on ability; the emphasis on the boy child is absolutely unnecessary because it does not add anything to ability. The paper further calls on the educated elite to enlighten their people to be aware that the dignity of the girl child is fundamentally, essentially and unquestionably equal to the dignity of the boy child. Finally, the paper concludes that, the male child has not in most cases fulfilled the long awaited expectations of being the second father in the house.


2020 ◽  
Vol 114 (3) ◽  
pp. e561
Author(s):  
Miguel Ruiz-Jorro ◽  
Minerva Ferrer-Buitrago ◽  
Juan Jesús Bataller-Sánchez ◽  
Antonio Barberá-Alberola ◽  
Xavier Vendrell-Montón ◽  
...  

Author(s):  
Arunoday Das ◽  
R. K. Biswas ◽  
B. C. Deka ◽  
D. J. Dutta

The objective of the present study was to find the comparative efficacy of three extenders to preserve semen of Labrador-Retriever (LR) dogs at 5o C for a short term. The semen samples of LR dogs were collected by digital manipulation method and extended at the rate of 1:4 in Tris-Egg Yolk- Citric Acid-Glucose (TEYCAG), Tris-Egg Yolk- Citric Acid-Fructose (TEYCAF) and Egg Yolk-Citrate-Glycine-Glucose (EYCGG) extenders by split sample technique. Semen was evaluated at 0, 24, 48, 72, 96 and 120 hours of preservation. Mean motile, live and HOST-reacted sperm and acrosomal, head, mid piece and tail abnormalities of spermatozoa varied significantly (PP less than 0.01) between extenders and between preservation periods. The interactions between extender and preservation period were also significant (P less than 0.01) except for HOST-reacted and head abnormalities of sperm. The highest mean motile, live and HOST-reacted sperm were recorded in TEYCAG extender which did not differ significantly from that of TEYCAF extender. Mean per cent sperm acrosomal and tail abnormalities were significantly (PP less than 0.05) lower, and the incidences of mean sperm head and mid piece abnormalities were also lower in TEYCAG, but not significantly from that in TEYCAF irrespective of hour of preservation. Per cent motile, live and HOST-reacted sperm were significantly (PP less than 0.05) lower and sperm acrosomal, head, mid piece and tail abnormalities were significantly (PP less than 0.05) higher in EYCGG as compared to that in TEYCAG and TEYCAF irrespective of hour of preservation. It was concluded that the semen of LR dog sustained good quality during preservation up to 5 days at 5oC suitable for successful artificial insemination and would be preserved better in TEYCAG and TEYCAF extenders than in EYCGG extender, since more than 50 per cent sperm motility and live sperm were maintained up to 120 hours of preservation in the former two extenders.


2019 ◽  
Vol 31 (10) ◽  
pp. 1545 ◽  
Author(s):  
Lucía Martínez-Fresneda ◽  
Emma O'Brien ◽  
Rosario Velázquez ◽  
Adolfo Toledano-Díaz ◽  
Carlos M. Martínez-Cáceres ◽  
...  

The aim of this study was to examine ovine sperm cryoresistance during the rutting season (RS) and its association with sperm head area and seminiferous epithelium proliferation. Small ruminants show fluctuating testosterone levels throughout the year, which could interfere with spermatogenesis and sperm cryopreservation. Ejaculates, testicular biopsies and blood were collected during the middle and at the end of the RS (Middle-RS vs End-RS) during periods of high and low testosterone levels in Merino and Mouflon rams. Fresh and frozen–thawed sperm quality, sperm morphometry, seminiferous tubule morphometry and testicular proliferation markers (proliferating cell nuclear antigen, proliferation marker protein Ki-67 and transcription factor GATA-4) were evaluated. Post-thaw sperm viability was higher in the End-RS group in both Merino (69.9±8.2 vs 41.6±7.3%; P=0.020) and Mouflon rams (40.9±3.3 vs 24.2±5.0%; P=0.008). Mouflons had larger sperm head area at the End-RS (38.3±0.2 vs 34.3±0.1µm2; P=0.029), whereas there was no difference between Merino groups (35.7±0.5 vs 34.8±1.0µm2). Seminiferous tubule morphometry and proliferation markers showed higher levels of germinal epithelium proliferation in the Middle-RS of both species. In conclusion, sperm freezability is affected during the RS in domestic and wild rams, which could be correlated with changes that occur during spermatogenesis, since there is an effect of season on cell proliferation in the testis.


Reproduction ◽  
2011 ◽  
Vol 142 (6) ◽  
pp. 819-830 ◽  
Author(s):  
Laura Gómez Montoto ◽  
María Varea Sánchez ◽  
Maximiliano Tourmente ◽  
Juan Martín-Coello ◽  
Juan José Luque-Larena ◽  
...  

Sperm competition favours an increase in sperm swimming velocity that maximises the chances that sperm will reach the ova before rival sperm and fertilise. Comparative studies have shown that the increase in sperm swimming speed is associated with an increase in total sperm size. However, it is not known which are the first evolutionary steps that lead to increases in sperm swimming velocity. Using a group of closely related muroid rodents that differ in levels of sperm competition, we here test the hypothesis that subtle changes in sperm design may represent early evolutionary changes that could make sperm swim faster. Our findings show that as sperm competition increases so does sperm swimming speed. Sperm swimming velocity is associated with the size of all sperm components. However, levels of sperm competition are only related to an increase in sperm head area. Such increase is a consequence of an increase in the length of the sperm head, and also of the presence of an apical hook in some of the species studied. These findings suggest that the presence of a hook may modify the sperm head in such a way that would help sperm swim faster and may also be advantageous if sperm with larger heads are better able to attach to the epithelial cells lining the lower isthmus of the oviduct where sperm remain quiescent before the final race to reach the site of fertilisation.


2018 ◽  
Vol 30 (4) ◽  
pp. 591 ◽  
Author(s):  
Ai Ushiyama ◽  
Atsushi Tajima ◽  
Naoto Ishikawa ◽  
Atsushi Asano

During cryopreservation, spermatozoa are subjected to cryodamage that leads to a decline in fertilisation ability. Due to the complex nature of this process, the initial trigger for cryodamage remains unknown. Recently, we demonstrated that cryopreservation induces early apoptotic changes characterised by phosphatidylserine (PS) translocation via sterol loss from the plasma membrane of chicken spermatozoa. This led us to hypothesise that sterol incorporation into membranes minimises cryodamage, thereby improving the quality of cryopreserved chicken spermatozoa. In the present study, treating spermatozoa with 1.5 mg mL−1 cholesterol- and 3 mg mL−1 desmosterol-loaded cyclodextrin (CLC and DLC respectively) increased post-thaw survival and motility. These effects appeared to be highly dependent the amount of sterol loaded into the spermatozoa. Localisation experiments confirmed the incorporation of exogenous cholesterol into the sperm head region. Detection of PS translocation showed that elevation of these sterols inhibited early apoptotic changes, thereby enhancing post-thaw survival. Furthermore, CLC and DLC treatment suppressed spontaneous acrosome reaction after cryopreservation, preserving the ability of spermatozoa to undergo acrosome reactions in response to physiological stimulation. These results demonstrate that loading sterols into chicken spermatozoa before cryopreservation enhances their quality by inhibiting early apoptotic changes and spontaneous acrosome reactions. The present study provides new mechanistic insight into cryodamage in chicken spermatozoa.


2021 ◽  
Vol 24 (3) ◽  
pp. 355-364
Author(s):  
M. G. Ivanova ◽  
B. A. Georgiev ◽  
P. S. Taushanova ◽  
D. G. Gradinarska ◽  
T. S. Tsvetkov ◽  
...  

The present research was focused on the differentiation of specific proteins in the seminal plasma (SP) of two horse breeds - Holsteiner (n=4) and East Bulgarian (n=4) and their relation with indivi­dual or breed characteristics, kinematic parameters of spermatozoa and the sperm head area. After CASA analysis of 8 ejaculates, no statistical differences in the kinematic parameters of the sperms between the two horse breeds were found out with the exception of the sperm head area (P<0.05), which can be considered as a morphometric marker of breed affiliation. The values ​​for rapid sperm in East Bulgarian and Holsteiners were 28.1±0.2 μm2 and 19.9±0.3 μm2 respectively. The chromatographic analysis demonstrated specific quantitative and qualitative protein content of the individual chromatographic peaks (11 for Holsteiner and 15 for the Eastern Bulgarian breed), with similarity to the basic proteins. Three specific proteins with a molecular mass of 76 kDa, 21.6 kDa and 24.3 kDa, were differentiated by SDS PAGE in the Holsteiner breed, whereas in the Eastern Bulgarian horse breed they had a lower protein mass - 30.1 kDa and 14.2 kDa and 12.6 kDa. In conclusion, differences in the specific protein profile of Holsteiner and Eastern Bulgarian horse breeds are individually and naturally determined without significant effect on sperm kinematics. The sperm head area was a breed-specific difference.


Zygote ◽  
2011 ◽  
Vol 21 (1) ◽  
pp. 65-70 ◽  
Author(s):  
Tomasz Lech ◽  
Aniela Golas ◽  
Jozefa Styrna

SummaryThe aim of the study was to evaluate the influence of the chromosome Y structure and Trp53 genotype on semen quality parameters. Mice with partial deletion of the Y chromosome (B10.BR-Ydel) have severely altered sperm head morphology when compared with males that possess the complete Y chromosome (B10.BR). Control males from B10.BR and B10.BR-Ydel mice, and mutant males from B10.BR-p53−/− and B10.BR-Ydel-p53−/− experimental groups were used. We assessed testis weight, sperm head abnormalities, viability of spermatozoa (eosin test), percentage of motile and immature sperm, and performed a hypo-osmotic test to detect abnormal tail membrane integrity. Sperm morphology and maturation were controlled by the genes within the deleted region of the Y chromosome. Testis weight was higher in the mutants than in the control males, possibly due to cell accumulation in Trp53-deficient males as the concentration of sperm was significantly increased in the mutants. An elevated percentage of abnormal sperm was noted in B10.BR-p53−/− and B10.BR-Ydel-p53−/− male mice. We suggest that, in Trp53-deficient mice, the sperm cells that escape apoptosis are the ones that have abnormal morphology. The only sperm quality parameter affected by the interplay between Trp53 and chromosome Y genes was sperm motility, which was elevated in B10.BR-p53−/− males, but remained unchanged in B10.BR-Ydel-p53−/− males.


2004 ◽  
Vol 16 (7) ◽  
pp. 681 ◽  
Author(s):  
T. Révay ◽  
S. Nagy ◽  
A. Kovács ◽  
M. E. Edvi ◽  
A. Hidas ◽  
...  

The head area of bull spermatozoa was measured after viability and acrosome staining using trypan blue and Giemsa stains, followed by X- and Y-chromosome-specific fluorescence in situ hybridisation (FISH). The former staining made possible the categorisation of cells according to morphology and membrane integrity, whereas the latter allowed distinction of spermatozoa bearing X- and Y-chromosomes. Individual spermatozoa could be followed during the consecutive steps of staining, measurement and FISH. Using a high-resolution digital imaging system and measurement software, the head area of more than 3000 cells of five bulls was determined precisely. In all bulls, morphologically normal, viable cells with intact acrosomes were significantly smaller than dead cells with damaged acrosomes. No significant difference in the head area between X- and Y-chromosome-bearing viable, acrosome-intact spermatozoa was found in individual bulls. However, significant between-bull differences were detected in all cell categories.


Author(s):  
Shailesh Pande ◽  
Vidya Salaskar ◽  
Anurita Pais ◽  
Gauri Pradhan ◽  
Smita Patil ◽  
...  

Background: Turner syndrome (TS) is the most common chromosomal abnormality reported in the females. Objective of this retrospective study was to determine the frequency of turner Syndrome and its various cytogenetic types in samples suspected to be of turner syndrome, received in the Department of Cytogenetics, Metropolis Healthcare laboratory, Mumbai, Maharashtra, India. Methods: The current study was performed on 935 clinically suspected samples with Turner Syndrome within the age group of 01-16 years. Peripheral blood (2-3 ml) in Sodium heparin Vacutainers was collected from all the patients, the cultures were set & analysed by GTG–banding at 450-550 band level Results were reported as per the guidelines of the International System for Human Cytogenomic Nomenclature (ISCN) and The College of American Pathologists (CAP) and National Accreditation Board for Testing and Calibration Laboratories (NABL).Results: In our study, out of the total 935 samples referred to Metropolis Healthcare Ltd, about 348 had cytogenetically turner or Turner variant findings. Further, out of 348 cases, 69 cases were detected to have presence of single X chromosome (19.83%), mosaic pattern in 116 cases (33.33%), presence of Y chromosome in 63 cases (18.10%) polymorphic variation in 58 cases (16.67%), presence of only isochromosome Xq in 9 cases (2.59%) and 33 cases (9.48%) with other or additional abnormalities. Conclusions: The cytogenetic confirmation and pattern of chromosomal aberration is very important as early detection may help to improve the quality of life especially in patients with cytogenetically Turner variant pattern with presence of Y chromosome. 


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