scholarly journals Comparative evaluation of seminal plasma proteins in Holsteiner and East Bulgarian horse breeds in relation to functional parameters of spermatozoa

2021 ◽  
Vol 24 (3) ◽  
pp. 355-364
Author(s):  
M. G. Ivanova ◽  
B. A. Georgiev ◽  
P. S. Taushanova ◽  
D. G. Gradinarska ◽  
T. S. Tsvetkov ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Protein Profile ◽  
Specific Protein ◽  
Sperm Head ◽  
Kinematic Parameters ◽  
Protein Mass ◽  
Specific Proteins ◽  
Chromatographic Peaks ◽  
The Individual ◽  
Head Area

The present research was focused on the differentiation of specific proteins in the seminal plasma (SP) of two horse breeds - Holsteiner (n=4) and East Bulgarian (n=4) and their relation with indivi­dual or breed characteristics, kinematic parameters of spermatozoa and the sperm head area. After CASA analysis of 8 ejaculates, no statistical differences in the kinematic parameters of the sperms between the two horse breeds were found out with the exception of the sperm head area (P<0.05), which can be considered as a morphometric marker of breed affiliation. The values ​​for rapid sperm in East Bulgarian and Holsteiners were 28.1±0.2 μm2 and 19.9±0.3 μm2 respectively. The chromatographic analysis demonstrated specific quantitative and qualitative protein content of the individual chromatographic peaks (11 for Holsteiner and 15 for the Eastern Bulgarian breed), with similarity to the basic proteins. Three specific proteins with a molecular mass of 76 kDa, 21.6 kDa and 24.3 kDa, were differentiated by SDS PAGE in the Holsteiner breed, whereas in the Eastern Bulgarian horse breed they had a lower protein mass - 30.1 kDa and 14.2 kDa and 12.6 kDa. In conclusion, differences in the specific protein profile of Holsteiner and Eastern Bulgarian horse breeds are individually and naturally determined without significant effect on sperm kinematics. The sperm head area was a breed-specific difference.

Еffect of different breeds on the protein profile in ram seminal plasma

2021 ◽  
Vol 24 (3) ◽  
pp. 344-354
Author(s):  
M. Ivanova ◽  
D. Gradinarska ◽  
S. Yotov ◽  
D. Abadjieva ◽  
Ts. Tzvetkov ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Protein Profile ◽  
Polyacrylamide Gel Electrophoresis ◽  
Biological Marker ◽  
Sds Page ◽  
Specific Proteins ◽  
Molecular Masses ◽  
Seminal Plasma Proteins ◽  
The Individual ◽  
Individual Profiles

In this study the individual profiles of seminal plasma proteins (SPP) in rams of three breeds – Merino, Pleven Blackhead and Ile de France - were analysed. The study was carried out with three rams at 3, 6 and 10 years of age, grown and fed under similar conditions. Eighteen ejaculates (6 ejaculates from each ram) were evaluated by Sperm Class Analyzer. The total SPP concentration was measured spectrophotometrically. The separation and characterisation of SPP was performed by HPLC and one dimensional polyacrylamide gel electrophoresis (SDS-PAGE). There were no significant differences between the characteristics of ejaculates and the main kinematic parameters of the sperm in the breeds studied. Chromatograms showed specific profiles with 9, 10 and 11 protein peaks for Merino, Pleven Blackhead and Ile de France breeds, respectively. The total SPP concentration was the highest in the Pleven Blackhead breed and the lowest in Ile de France breed. The major parts of SPP in the three breeds were identical. The seminal plasma of Merino breed contained proteins with molecular mass of 30.3 kDa, 15.7 kDa and 15.2 kDa that were not present in the other two breeds. In the Ile de France and Pleven Blackhead samples only, two proteins with molecular masses of 39.7 kDa and 21.1 kDa, were observed. In conclusion, the detection of specific proteins can be used as a biological marker for sheep breed identification.

Isotope dilution--mass spectrometric quantification of specific proteins: model application with apolipoprotein A-I

1996 ◽  
Vol 42 (10) ◽  
pp. 1676-1682 ◽  
Author(s):  
J R Barr ◽  
V L Maggio ◽  
D G Patterson ◽  
G R Cooper ◽  
L O Henderson ◽  
...  
Keyword(s):  
Isotope Dilution ◽  
Primary Standard ◽  
Specific Protein ◽  
Mass Spectrometric ◽  
Spectrometric Method ◽  
Mass Spectrometric Method ◽  
Protein Mass ◽  
Specific Proteins ◽  
Total Protein Mass ◽  
Model Approach

Abstract An enzymatic hydrolysis isotope dilution-mass spectrometric method was developed for reference quantification of specific proteins. The analytical procedure involved measuring a reproducibly hydrolyzed peptide (serving as the primary standard) unique to a specific protein. This new mass spectrometric method was evaluated by assessing the concentration of apolipoprotein (apo) A-I in the European Community Bureau of Reference (BCR) lyophilized Certified Reference Material (CRM 393). We used the method to make 96 measurements (4 replicate analyses of 4 enzymatic digests of 6 vials of BCR-CRM 393), which gave an average total protein mass of 1.048 mg (+/- 1.0% at 99% confidence limits). The total overall analytical CV was 3.95%. The results of this evaluation of our model approach to determine the concentration of a specific protein in a purified preparation demonstrated that our new mass spectrometric method can be used to measure apolipoproteins and other specific proteins without the use of epitopic immunoassay methods.

scholarly journals Snail mucus from the mantle and foot of two land snails, Lissachatina fulica and Hemiplecta distincta, exhibits different protein profile and biological activity

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Nattaphop Noothuan ◽  
Kantamas Apitanyasai ◽  
Somsak Panha ◽  
Anchalee Tassanakajon
Keyword(s):  
Biological Activities ◽  
Protein Pattern ◽  
Protein Profile ◽  
Antibacterial Properties ◽  
Biological Properties ◽  
Specific Protein ◽  
Land Snails ◽  
Snail Species ◽  
Significant Difference ◽  
Different Types

Abstract Objective Snails secrete different types of mucus that serve several functions, and are increasingly being exploited for medical and cosmetic applications. In this study, we explored the protein pattern and compared the biological properties of the mucus secreted from the mantle collar and foot of two snail species, Lissachatina fulica and Hemiplecta distincta. Result Protein profile showed a different pattern between the two species and between the two secretory parts. The mantle-specific protein bands were further characterized and among them was an antibacterial protein, achacin. Accordingly, the mucus from the mantle exhibited the higher antibacterial activity than that from the foot in both snail species. The mucus from H. distincta, first reported here, also showed antibacterial properties, but with a lower activity compared to that for L. fulica. Snail mucus also exhibited anti-tyrosinase activity and antioxidant activity but with no significant difference between the foot and mantle mucus. These results indicate some different protein compositions and biological activities of snail slime from the mantle and foot, which might be associated with their specific functions in the animal and are useful for medical applications.

Collection frequency affects percent Y-chromosome bearing sperm, sperm head area and quality of bovine ejaculates

2002 ◽  
Vol 57 (4) ◽  
pp. 1327-1346 ◽  
Author(s):  
John E Chandler ◽  
Anita M Canal ◽  
J.B Paul ◽  
E.B Moser
Keyword(s):  
Y Chromosome ◽  
Sperm Head ◽  
Head Area

scholarly journals Generation of asymmetry during development. Segregation of type-specific proteins in Caulobacter.

1979 ◽  
Vol 81 (1) ◽  
pp. 123-136 ◽  
Author(s):  
N Agabian ◽  
M Evinger ◽  
G Parker
Keyword(s):  
Cell Cycle ◽  
Cell Division ◽  
Messenger Rna ◽  
Cell Types ◽  
Specific Protein ◽  
Soluble Proteins ◽  
Specific Cell ◽  
Daughter Cells ◽  
Specific Proteins ◽  
Entire Cell

An essential event in developmental processes is the introduction of asymmetry into an otherwise undifferentiated cell population. Cell division in Caulobacter is asymmetric; the progeny cells are structurally different and follow different sequences of development, thus providing a useful model system for the study of differentiation. Because the progeny cells are different from one another, there must be a segregation of morphogenetic and informational components at some time in the cell cycle. We have examined the pattern of specific protein segregation between Caulobacter stalked and swarmer daughter cells, with the rationale that such a progeny analysis would identify both structurally and developmentally important proteins. To complement the study, we have also examined the pattern of protein synthesis during synchronous growth and in various cellular fractions. We show here, for the first time, that the association of proteins with a specific cell type may result not only from their periodicity of synthesis, but also from their pattern of distribution at the time of cell division. Several membrane-associated and soluble proteins are segregated asymmetrically between progeny stalked and swarmer cells. The data further show that a subclass of soluble proteins becomes associated with the membrane of the progeny stalked cells. Therefore, although the principal differentiated cell types possess different synthetic capabilities and characteristic proteins, the asymmetry between progeny stalked and swarmer cells is generated primarily by the preferential association of specific soluble proteins with the membrane of only one daughter cell. The majority of the proteins which exhibit this segregation behavior are synthesized during the entire cell cycle and exhibit relatively long, functional messenger RNA half-lives.

scholarly journals Photoreceptor Compartment-Specific TULP1 Interactomes

2021 ◽  
Author(s):  
Lindsey A Ebke ◽  
Satyabrata Sinha ◽  
Gayle JT Pauer ◽  
Stephanie A Hagstrom
Keyword(s):  
Molecular Motors ◽  
Protein Trafficking ◽  
Specific Interaction ◽  
Specific Protein ◽  
Vesicle Recycling ◽  
Binding Partners ◽  
Skeletal Protein ◽  
Liquid Chromatography Tandem Mass ◽  
In The Wild ◽  
Specific Proteins

Photoreceptors are highly compartmentalized cells with large amounts of proteins synthesized in the inner segment (IS) and transported to the outer segment (OS) and synaptic terminal. Tulp1 is a photoreceptor-specific protein localized to the IS and synapse. In the absence of Tulp1, sev-eral OS-specific proteins are mislocalized and synaptic vesicle recycling is impaired. To better understand the involvement of Tulp1 in protein trafficking, our approach was to physically iso-late Tulp1-containing photoreceptor compartments by serial tangential sectioning of retinas and to identify compartment-specific Tulp1 binding partners by immunoprecipitation followed by liquid chromatography tandem mass spectrometry. Our results indicate that Tulp1 has two dis-tinct interactomes. We report the identification of: 1) an IS-specific interaction between Tulp1 and the motor protein Kinesin family member 3a (Kif3a), 2) a synaptic-specific interaction be-tween Tulp1 and the scaffold protein Ribeye, and 3) an interaction between Tulp1 and the cyto-skeletal protein Microtubule-associated protein 1B (MAP1B) in both compartments. Immuno-localization studies in the wild-type retina indicate that Tulp1 and its binding partners co-localize to their respective compartments. Our observations are compatible with Tulp1 functioning in protein trafficking in multiple photoreceptor compartments, likely as an adapter molecule linking vesicles to molecular motors and the cytoskeletal scaffold.

INCREASED AVERAGE OF SPERM HEAD AREA (ASHA) IS A NOVEL SPERM PARAMETER ASSOCIATED WITH HIGHER INCIDENCE OF SPERM ANEUPLOIDY

2020 ◽  
Vol 114 (3) ◽  
pp. e561
Author(s):  
Miguel Ruiz-Jorro ◽  
Minerva Ferrer-Buitrago ◽  
Juan Jesús Bataller-Sánchez ◽  
Antonio Barberá-Alberola ◽  
Xavier Vendrell-Montón ◽  
...  
Keyword(s):  
Sperm Head ◽  
Sperm Parameter ◽  
Sperm Aneuploidy ◽  
Head Area

scholarly journals Modular protein-oligonucleotide signal exchange

2020 ◽  
Vol 48 (12) ◽  
pp. 6431-6444
Author(s):  
Deepak K Agrawal ◽  
Rebecca Schulman
Keyword(s):  
Specific Protein ◽  
Vascular Endothelial ◽  
Complementary Dna ◽  
Exchange Processes ◽  
Dna Strand Displacement ◽  
Specific Proteins ◽  
Dna Strand ◽  
Endothelial Growth Factor ◽  
Signal Exchange

Abstract While many methods are available to measure the concentrations of proteins in solution, the development of a method to quantitatively report both increases and decreases in different protein concentrations in real-time using changes in the concentrations of other molecules, such as DNA outputs, has remained a challenge. Here, we present a biomolecular reaction process that reports the concentration of an input protein in situ as the concentration of an output DNA oligonucleotide strand. This method uses DNA oligonucleotide aptamers that bind either to a specific protein selectively or to a complementary DNA oligonucleotide reversibly using toehold-mediated DNA strand-displacement. It is possible to choose the sequence of output strand almost independent of the sensing protein. Using this strategy, we implemented four different exchange processes to report the concentrations of clinically relevant human α-thrombin and vascular endothelial growth factor using changes in concentrations of DNA oligonucleotide outputs. These exchange processes can operate in tandem such that the same or different output signals can indicate changes in concentration of distinct or identical input proteins. The simplicity of our approach suggests a pathway to build devices that can direct diverse output responses in response to changes in concentrations of specific proteins.

scholarly journals Plasma concentrations of platelet-specific proteins correlated with platelet survival

Blood ◽  
1980 ◽  
Vol 55 (1) ◽  
pp. 82-84 ◽  
Author(s):  
DJ Doyle ◽  
CN Chesterman ◽  
JF Cade ◽  
JR McGready ◽  
GC Rennie ◽  
...  
Keyword(s):  
Life Span ◽  
Plasma Concentrations ◽  
Specific Protein ◽  
Platelet Factor ◽  
In Vivo Release ◽  
Platelet Survival ◽  
Specific Proteins ◽  
Artery Disease ◽  
Multiple Hit

Abstract Relationships between 51Cr platelet survival and plasma concentrations of beta-thromboglobulin (betaTG) and platelet factor 4 (PF4) were analyzed in 91 studies of patients with coronary artery disease. betaTG was significantly correlated with platelet life-span, turnover, and the number of hits in the multiple hit model. PF4 was significantly correlated with life-span and turnover. The most significant relationship involving platelet-specific protein concentrations and life-span estimates was between betaTG and life-span estimated using the multiple hit model (r = -0.39, p less than 0.001). There was a high correlation between betaTG and PF4 (r = 0.62, p less than 0.001), and no improvement could be obtained by combining the measurements of the two proteins in any regression with life-span or turnover. The results indicate that the patients with the shortest platelet survival time in this group tended to have the highest plasma concentration of betaTG and PF4 and thus probably increased in vivo release of betaTG and PF4. They strengthen the claim that these platelet-specific proteins may be indicators of platelet involvement in disease.

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