Computer Databases: Uses In Clinical and Academic Practice

1995 ◽  
Vol 112 (5) ◽  
pp. P80-P80
Author(s):  
Eric E. Smouha ◽  
Franklin M. Rizer
Keyword(s):  
De Novo ◽  
Clinical Applications ◽  
Educational Objectives ◽  
Academic Practice ◽  
Database Program

Educational objectives: To understand the fundamental concepts of organizing data on a computer and to develop clinical applications de novo on a commercially available database program.

Trio deep-sequencing does not reveal unexpected mutations in Cas9-edited monkeys

2018 ◽  
Author(s):  
Xin Luo ◽  
Yaoxi He ◽  
Chao Zhang ◽  
Xiechao He ◽  
Lanzhen Yan ◽  
...  
Keyword(s):  
Genome Editing ◽  
Deep Sequencing ◽  
De Novo ◽  
Clinical Applications ◽  
Preclinical Model ◽  
Whole Genome ◽  
Spontaneous Mutations ◽  
Target Effect

CRISPR-Cas9 is a widely-used genome editing tool, but its off-target effect remains a concern, especially in view of future clinical applications. Non-human primates (NHPs) share close genetic and physiological similarities with humans, making them an ideal preclinical model for developing Cas9-based therapies. However, no comprehensive in vivo off-target assessment has been conducted in NHPs. Here we performed whole genome trio sequencing of Cas9-treated monkeys. We found they only carried a small number of de novo mutations that can be explained by expected spontaneous mutations, and no unexpected mutations were detected.

Clinical Applications of Electronystagmography

1995 ◽  
Vol 112 (5) ◽  
pp. P130-P131
Author(s):  
Alfred C. Coats
Keyword(s):  
Clinical Applications ◽  
Educational Objectives ◽  
Clinical Interpretation

Educational objectives: To understand the principles underlying the techniques and procedures of the ENG examination and the clinical interpretation of the ENG examination.

Clinical Applications of Otoacoustic Emissions

1995 ◽  
Vol 112 (5) ◽  
pp. P23-P23 ◽  
Author(s):  
Brenda L. Lonsbury-Martin ◽  
Thomas Balkany ◽  
Fred F. Telischi ◽  
Glen K. Martin
Keyword(s):  
Otoacoustic Emissions ◽  
Clinical Utility ◽  
Otoacoustic Emission ◽  
Clinical Applications ◽  
Educational Objectives

Educational objectives: To understand the fundamental basis of the cochlear generation of otoacoustic emissions and to appreciate the clinical utility of the recognized types of otoacoustic emission.

scholarly journals Vers une thérapie génique pour la progéria ?

2021 ◽  
Vol 37 (4) ◽  
pp. 413-416
Author(s):  
Bertrand Jordan
Keyword(s):  
Mouse Model ◽  
Point Mutation ◽  
Cell Lines ◽  
De Novo ◽  
Clinical Applications ◽  
Premature Aging ◽  
Base Editing ◽  
Complete Reversal ◽  
Thérapie Génique ◽  
Patient Cell

Hutchinson-Gilford Progeria (acute premature aging) is caused by a de novo point mutation in the lamin A gene. Recently, this mutation has been accurately corrected by base editing in patient cell lines and in a mouse model, resulting in nearly complete reversal to a normal phenotype. This success opens the perspective for clinical applications in Progeria and other diseases.

scholarly journals Vascular Endothelial Growth Factor, from Basic Research to Clinical Applications

2018 ◽  
Vol 19 (12) ◽  
pp. 3750 ◽  
Author(s):  
Kurt Ballmer-Hofer
Keyword(s):  
Vascular Endothelial Growth Factor ◽  
Growth Factors ◽  
Growth Factor ◽  
Tumor Cells ◽  
Specific Growth ◽  
De Novo ◽  
Basic Research ◽  
Clinical Applications ◽  
Vascular Endothelial ◽  
Endothelial Growth Factor

Judah Folkman’s landmark discovery in the 1970s showing that tumors, growing beyond a few millimeters in diameter, depend on de novo vascularization triggered by specific growth factors released by tumor cells encountering hypoxia [...]

The Morphology of the Rat Kidney Following Folic Acid Administration

1970 ◽  
Vol 28 ◽  
pp. 200-201
Author(s):  
Aline Byrnes ◽  
Elsa E. Ramos ◽  
Minoru Suzuki ◽  
E.D. Mayfield
Keyword(s):  
Folic Acid ◽  
De Novo ◽  
Specific Activity ◽  
Rat Kidney ◽  
Present Report ◽  
Intracellular Localization ◽  
Male Rats ◽  
Renal Hypertrophy ◽  
Electron Microscopic ◽  
Biochemical Alterations

Renal hypertrophy was induced in 100 g male rats by the injection of 250 mg folic acid (FA) dissolved in 0.3 M NaHCO3/kg body weight (i.v.). Preliminary studies of the biochemical alterations in ribonucleic acid (RNA) metabolism of the renal tissue have been reported recently (1). They are: RNA content and concentration, orotic acid-c14 incorporation into RNA and acid soluble nucleotide pool, intracellular localization of the newly synthesized RNA, and the specific activity of enzymes of the de novo pyrimidine biosynthesis pathway. The present report describes the light and electron microscopic observations in these animals. For light microscopy, kidney slices were fixed in formalin, embedded, sectioned, and stained with H & E and PAS.

Specimen Preparation for Clinical Applications of X-Ray Microanalysis

1990 ◽  
Vol 48 (2) ◽  
pp. 328-329
Author(s):  
J.D. Shelburne ◽  
G.M. Roomans
Keyword(s):  
Carbon Coating ◽  
Clinical Applications ◽  
Point Of View ◽  
Practical Significance ◽  
Sweat Test ◽  
Specimen Preparation ◽  
Tissue Preparation ◽  
X Ray ◽  
Gallbladder Stones ◽  
Clinical Problems

Proper preparative procedures are a prerequisite for the validity of the results of x-ray microanalysis of biological tissue. Clinical applications of x-ray microanalysis are often concerned with diagnostic problems and the results may have profound practical significance for the patient. From this point of view it is especially important that specimen preparation for clinical applications is carried out correctly.Some clinical problems require very little tissue preparation. Hair, nails, and kidney and gallbladder stones may be examined and analyzed after carbon coating. High levels of zinc or copper in hair may be indicative of dermatological or systemic diseases. Nail clippings may be analyzed (as an alternative to the more conventional sweat test) to confirm a diagnosis of cystic fibrosis. X-ray microanalysis in combination with scanning electron microscopy has been shown to be the most reliable method for the identification of the components of kidney or gallbladder stones.A quantitatively very important clinical application of x-ray microanalysis is the identification and quantification of asbestos and other exogenous particles in lung.

Reduction of Potassium in Kidney Proximal Tubules to Aid in Electron Probe X-Ray Microanalysis of Calcium

1985 ◽  
Vol 43 ◽  
pp. 474-475
Author(s):  
A. LeFurgey ◽  
P. Ingram ◽  
L.J. Mandel
Keyword(s):  
Smooth Muscle ◽  
Proximal Tubule ◽  
Electron Probe ◽  
Clinical Applications ◽  
Proximal Tubules ◽  
Rabbit Kidney ◽  
Target Cells ◽  
X Ray ◽  
Freeze Dried

For quantitative determination of subcellular Ca distribution by electron probe x-ray microanalysis, decreasing (and/or eliminating) the K content of the cell maximizes the ability to accurately separate the overlapping K Kß and Ca Kα peaks in the x-ray spectra. For example, rubidium has been effectively substituted for potassium in smooth muscle cells, thus giving an improvement in calcium measurements. Ouabain, a cardiac glycoside widely used in experimental and clinical applications, inhibits Na-K ATPase at the cell membrane and thus alters the cytoplasmic ion (Na,K) content of target cells. In epithelial cells primarily involved in active transport, such as the proximal tubule of the rabbit kidney, ouabain rapidly (t1/2= 2 mins) causes a decrease2 in intracellular K, but does not change intracellular total or free Ca for up to 30 mins. In the present study we have taken advantage of this effect of ouabain to determine the mitochondrial and cytoplasmic Ca content in freeze-dried cryosections of kidney proximal tubule by electron probe x-ray microanalysis.

Early ossicle growth in the regenerating disc of a brittle star

1994 ◽  
Vol 52 ◽  
pp. 364-365
Author(s):  
M. Shlepr ◽  
R. L. Turner
Keyword(s):  
Cell Membrane ◽  
Light Microscopy ◽  
De Novo ◽  
Current Model ◽  
Syncytium Formation ◽  
Brittle Star ◽  
Intracellular Location ◽  
Limited Volume ◽  
Tissue Calcification

Calcification in the echinoderms occurs within a limited-volume cavity enclosed by cytoplasmic extensions of the mineral depositing cells, the sclerocytes. The current model of this process maintains that the sheath formed from these cytoplasmic extensions is syncytial. Prior studies indicate that syncytium formation might be dependent on sclerocyte density and not required for calcification. This model further envisions that ossicles formed de novo nucleate and grow intracellularly until the ossicle effectively outgrows the vacuole. Continued ossicle growth occurs within the sheath but external to the cell membrane. The initial intracellular location has been confirmed only for elements of the echinoid tooth.The regenerating aboral disc integument of ophiophragmus filograneus was used to test the current echinoderm calcification model. This tissue is free of calcite fragments, thus avoiding questions of cellular engulfment, and ossicles are formed de novo. The tissue calcification pattern was followed by light microscopy in both living and fixed preparations.

Simple teaching aid with clinical applications

1976 ◽  
Vol 40 (2) ◽  
pp. 106-106
Author(s):  
DL Torney ◽  
HD Espinosa
Keyword(s):  
Clinical Applications ◽  
Teaching Aid
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