Antioxidant activity of extracts from Lavandula vera MM cell cultures

The antioxidant and metal chelating activities inJ. curcasprotein hydrolysates have been determined. The hydrolysates were produced by treatment of a nontoxic genotype with the digestive enzymes pepsin and pancreatin and then were characterized by fast protein liquid chromatography and reverse phase chromatography. Peptidic fractions with higher radical scavenging activity were analysed by matrix-assisted laser desorption/ionization mass spectrometry. The antioxidant activity was determined by measuring inhibition of the oxidative degradation ofβ-carotene and by measuring the reactive oxygen species (ROS) in Caco-2 cell cultures. Cu2+and Fe2+chelating activities were also determined. The hydrolysates inhibited the degradation ofβ-carotene and the formation of ROS in Caco-2 cells. The lower molecular weight peptidic fractions from FPLC had stronger antioxidant activity in cell cultures compared with the hydrolysates, which correlated with a higher content in antioxidant and chelating amino acids. These fractions were characterized by a large presence of peptides with different molecular masses. The hydrolysates exhibited both Cu2+and Fe2+chelating activity. It was concluded thatJ. curcasis a good source of antioxidant and metal chelating peptides, which may have a positive impact on the economic value of this crop, as a potential source of food functional components.

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Comparison of antioxidant activity between cyanidin-3-O-glucoside (C3G) liposome and cyanidin-3-O-glucoside (C3G) in 2D and 3D cell cultures

10.1101/314369 ◽

2018 ◽

Author(s):

Tisong Liang ◽

Rongfa Guan ◽

Guozhou Cao ◽

Haitao Shen ◽

Zhenfeng Liu ◽

...

Keyword(s):

Cell Culture ◽

Antioxidant Activity ◽

Cell Cultures ◽

Cell Model ◽

3D Cell Culture ◽

Culture Model ◽

Mda Content ◽

2D And 3D ◽

Cells Cultured

ABSTRACTThe 2D cell culture is the predominant in vitro model for numerous studies. However, 2D cell cultures may not accurately reflect the functions of three-dimensional (3D) tissues, which have extensive cell–cell and cell–matrix interactions; thus, using 2D cell cultures may lead to inaccurate experimental results. Therefore, to obtain adequate and detailed information about the antioxidant activity of cyanidin-3-O-glucoside (C3G) and C3G liposomes in the 2D and 3D cell culture models, we used in this study H2O2to construct the cell damage model and assess the antioxidant activity of C3G and C3G liposomes on Caco-2 cells cultured in the 3D model. We also measured the cell viability, cell morphology, and activity of glutathione (GSH), superoxide dismutase (SOD), total antioxidant capacity (T-AOC), and malondialdehyde (MDA) content of Caco-2 cells treated with H2O2, C3G, and C3G liposomes. Results showed that cells cultured in the 3D culture model formed a 3D structure and tight spheroids and showed increased cell activity and IC50. The C3G and C3G liposomes can enhance the activity of GSH, SOD, and T-AOC but decrease the MDA content. At the same time, the effect was more obvious in the 3D cell culture model than in the cells cultured in the 2D model. This study revealed that the results obtained from the 2D cell model may be inaccurate compared with the results obtained from the 3D cell model. A realistic mechanism study of antioxidant activity of C3G and C3G liposomes in the 3D cell model, which acts as an intermediate stage bridging the in vitro 2D and in vivo models, was observed.

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Antioxidant activity of ethanolic extract of inflorescence of Ormenis Africana in vitro and in cell cultures

Lipids in Health and Disease ◽

10.1186/1476-511x-10-78 ◽

2011 ◽

Vol 10 (1) ◽

pp. 78 ◽

Cited By ~ 13

Author(s):

Riadh Ben Mansour ◽

Bochra Gargouri ◽

Mohamed Bouaziz ◽

Nésrine Elloumi ◽

Imtinène Belhadj Jilani ◽

...

Keyword(s):

Antioxidant Activity ◽

Cell Cultures ◽

Ethanolic Extract

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Total Phenolic Content and Antioxidant Activity of Standardized Extracts from Leaves and Cell Cultures of Three Callistemon Species

American Journal of Plant Sciences ◽

10.4236/ajps.2011.26100 ◽

2011 ◽

Vol 02 (06) ◽

pp. 847-850 ◽

Cited By ~ 22

Author(s):

Mohamed I. S. Abdelhady ◽

Amira Abdel Motaal ◽

Ludger Beerhues

Keyword(s):

Antioxidant Activity ◽

Cell Cultures ◽

Total Phenolic Content ◽

Phenolic Content ◽

Total Phenolic

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Antioxidant Activity of the Stilbene Astringin, Newly Extracted from Vitis vinifera Cell Cultures

Clinical Chemistry ◽

10.1093/clinchem/43.6.1092 ◽

1997 ◽

Vol 43 (6) ◽

pp. 1092-1093 ◽

Cited By ~ 38

Author(s):

Jean-Michel Mérillon ◽

Bernard Fauconneau ◽

Pierre Waffo Teguo ◽

Laurence Barrier ◽

Joseph Vercauteren ◽

...

Keyword(s):

Antioxidant Activity ◽

Vitis Vinifera ◽

Cell Cultures

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Geroprotective potential of in vitro bioactive compounds isolated from yarrow (Achilleae millefolii L.) cell cultures

Foods and raw materials ◽

10.21603/2308-4057-2021-1-126-134 ◽

2021 ◽

pp. 126-134

Author(s):

Lyudmila Asyakina ◽

Natal’ya Fotina ◽

Natalia Izgarysheva ◽

Anatoliy Slavyanskiy ◽

Olga Neverova

Keyword(s):

Antioxidant Activity ◽

Suspension Culture ◽

Essential Oils ◽

Caffeic Acid ◽

Bioactive Compounds ◽

Cell Cultures ◽

Premature Aging ◽

Hairy Root Cultures ◽

Root Cultures

Introduction. There is an urgent need for geroprotectors that prevent premature aging, especially antioxidants of plant origin. Due to the shortage of medicinal plant materials, scientists look for alternative sources of bioactive compounds of phenolic nature, for example, cell cultures and organs of higher plants. This paper describes a study of the geroprotective potential of in vitro bioactive compounds isolated from yarrow (Achilleae millefolii L.) cell cultures. Study objects and methods. Callus, suspension and hairy root cultures of A. millefolii were obtained by in vitro cultivation on modified nutrient media. High performance liquid chromatography (HPLC) was used to analyze the composition of the cell cultures and ethanol extracts. The extracts’ antimicrobial activity was studied by the disk diffusion method and their antioxidant activity was measured based on titration of a potassium permanganate solution. Results and discussion. The biomass of all yarrow cell cultures contained essential oils, flavonoids, glycosides, phenolic acids, carotenoids, as well as vitamins C and E. The suspension culture had a higher content of essential oils, flavonoids and glycosides than the callus and hairy root cultures. The extracts of the A. millefolii suspension culture also contained geroprotectors – phenylpropanoids, flavonoids, and simple phenols, with a prevalence of caffeic acid, cynaroside, 4,5-dicofeylquinic acid, apigenin, and luteolin. In addition, HPLC revealed the presence of cumic aldehyde, umbelliferone, 3-caffeylquinic acid, and caffeic acid – the bioactive compounds previously not reported in yarrow. In vitro experiments with the extracts proved their antimicrobial and antioxidant activity. Conclusion. The complex of bioactive compounds isolated from the biomass of yarrow suspension culture provides this plant with potential geroprotective properties. Thus, yarrow can be used to create nutraceuticals that prevent premature aging.

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