cDNA cloning and analysis of RNA 2 of a Prunus stem pitting isolate of tomato ringspot virus

The ability of 32P-labeled transcribed cRNA probes to detect tomato ringspot virus (TmRSV) RNA in nucleic acid extracts from roots, bark, and leaves of nectarine (Prunus persica [L.] Batsch) trees with the Prunus stem-pitting disease was assessed and compared with detection of TmRSV antigen by enzyme-linked immunosorbent assay (ELISA) in the same tissues. Neither TmRSV-specific nucleic acid nor antigen was detected in nectarine leaf tissue. ELISA detected TmRSV antigen in root extracts from 71% of the diseased trees, while dot hybridization detected virus-specific nucleic acid in 18% of the same samples. However, ELISA detected TmRSV antigen in only 47% of bark extracts; whereas TmRSV-specific nucleic acid was detected in 100% of the bark extracts from samples collected at or near the soil line. When nucleic acid extracts from bark were prepared from various locations on diseased trees and tested for TmRSV-specific nucleic acid by dot hybridization, there was an almost perfect correlation between the presence of stem-pitting symptoms and the detection of TmRSV nucleic acid. Detection of TmRSV RNA from the bark tissue of rootstock suckers from TmRSV-infected `Delicious'/MM.lO6 apple (Malus × domestica Borkh.) trees was unsuccessful using dot hybridization. The viral RNA, however, was usually detected in either leaf or root tissue of these same trees.

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A threat to stone fruit and grape production: tomato ringspot virus (ToRSV) transmission by X. americanum s.l. (sensu lato).

10.1079/9781789247541.0029 ◽

2021 ◽

pp. 207-214

Author(s):

John M. Halbrendt

Keyword(s):

Integrated Management ◽

Economic Importance ◽

Ringspot Virus ◽

Stone Fruit ◽

Future Research ◽

Tomato Ringspot Virus ◽

Management Optimization ◽

Grape Varieties ◽

Stem Pitting ◽

Damage Symptoms

Abstract Prunus stem pitting (PSP) is a lethal disease caused by the Tomato ringspot virus (ToRSV) and transmitted by the dagger nematode. All peach varieties and most other stone fruit are susceptible to ToRSV. The same virus also causes a decline in certain wine grape varieties. In order to develop strategies to prevent the occurrence of these diseases it is important to understand the interaction between Xiphinema americanum and ToRSV. This chapter discusses the economic importance, geographical distribution, host range, damage symptoms, biology and life cycle, interactions with other nematodes and pathogens, recommended integrated management, and management optimization of X. americanum. Future research requirements and future developments are also mentioned.

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Survey for Viruses of Grapevine in Oregon and Washington

Plant Disease ◽

10.1094/pd-89-0763 ◽

2005 ◽

Vol 89 (7) ◽

pp. 763-766 ◽

Cited By ~ 44

Author(s):

R. R. Martin ◽

K. C. Eastwell ◽

A. Wagner ◽

S. Lamprecht ◽

I. E. Tzanetakis

Keyword(s):

Mosaic Virus ◽

Ringspot Virus ◽

Wine Grape ◽

Grapevine Fanleaf Virus ◽

Arabis Mosaic Virus ◽

Field Isolates ◽

Tomato Ringspot Virus ◽

Rupestris Stem Pitting ◽

Stem Pitting ◽

Vitis Spp

Grapevines (Vitis spp.) in Washington and Oregon were surveyed for the prevalence of key grapevine viruses. Samples collected from 1,522 vines in Washington were tested for Rupestris stem pitting associated virus (RSPaV), Grapevine fanleaf virus (GFLV), Arabis mosaic virus (ArMV), Tomato ringspot virus (ToRSV), and Grapevine leafroll associated virus-3 (GLRaV-3). Tests were also conducted for GLRaV-1 and -2 on 420 samples from Washington. Two hundred forty samples collected from wine grape vineyards in Oregon were tested for GLRaV-1, -2, and -3, and an additional 2,880 samples were collected from 40 vineyards known to have high populations of Xiphinema americanum nematodes. The latter were tested for ArMV, ToRSV, and GFLV. GLRaV-1, -2, and -3 were detected in 2.6, 0.2, and 6.5% of the Washington samples and in 3.0, 0.4, and 4.4% of the Oregon samples. RSPaV was detected in 4.6% of the samples from Washington. No ToRSV, ArMV, or GFLV was detected in any of the samples from Oregon or Washington. Transmission of field isolates of GLRaV-3 from Washington by the grape mealybug also was demonstrated.

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Detection of tomato ringspot virus in white ash and adjacent vegetation in central New York

Canadian Journal of Forest Research ◽

10.1139/x88-124 ◽

1988 ◽

Vol 18 (6) ◽

pp. 815-819 ◽

Cited By ~ 7

Author(s):

Martha A. Ferris ◽

John D. Castello

Keyword(s):

New York ◽

Plant Species ◽

Ringspot Virus ◽

Herbaceous Plant ◽

Tissue Samples ◽

White Ash ◽

Tomato Ringspot Virus ◽

Central New York

Tissue samples from 15 woody and herbaceous plant species, including white ash (Fraxinusamericana L.), were collected monthly during the spring and summer of 1985 and indexed by ELISA for tomato ringspot virus. The virus was detected in 12 of 23 white ash indexed, most frequently in the roots. Of 88 white ash seedlings inoculated with purified tomato ringspot virus, the virus was detected by ELISA in 7 seedlings after two cycles of growth and dormancy, and in only 1 seedling after one cycle of growth and dormancy postinoculation. The virus was also detected in Fragariavirginiana Mill., Prunusvirginiana L., Rubusoccidentalis L., R. strigosus Michx., Solidagocanadensis L., Trifoliumpratense L., T. repens L., and Daucuscarota L. growing within 50 m of infected ash.

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Nucleotide sequence of tomato ringspot virus RNA1

Journal of General Virology ◽

10.1099/0022-1317-76-2-465 ◽

1995 ◽

Vol 76 (2) ◽

pp. 465-473 ◽

Cited By ~ 44

Author(s):

M. E. Rott ◽

A. Gilchrist ◽

L. Lee ◽

D. Rochon

Keyword(s):

Nucleotide Sequence ◽

Ringspot Virus ◽

Tomato Ringspot Virus

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Grapevine yellow speckle — a newly recognized graft-transmissible disease of Vitis

Australian Journal of Agricultural Research ◽

10.1071/ar9720447 ◽

1972 ◽

Vol 23 (3) ◽

pp. 447 ◽

Cited By ~ 15

Author(s):

RH Taylor ◽

RC Woodham

Keyword(s):

Seed Transmission ◽

Ringspot Virus ◽

Herbaceous Plants ◽

Grapevine Fanleaf Virus ◽

The Past ◽

Tomato Ringspot Virus ◽

Wide Range ◽

Heat Therapy ◽

Transmissible Disease ◽

Leaf Symptoms

Evidence is presented to show that many grapevines showing leaf symptoms which, particularly when intense, cannot be distinguished from those caused by strains of grapevine fanleaf virus (GFV) or tomato ringspot virus (TRSV) are infected with a previously undescribed disease for which the name grapevine yellow speckle (GYS) is proposed. The causal agent of the disease has not been isolated, but it is graft-transmissible from infected to healthy grapevines. It has not been transmitted by sap inoculation to herbaceous plants. The disease has been detected in some grapevine cultivars grown in Australia for many years, and also in several other cultivars and rootstocks imported from California over the past 10 years. There is no evidence of natural spread or of seed transmission. Attempts to eliminate GYS from plants by heat therapy and propagation of tip cuttings have been unsuccessful. Mataro and LN33, the best indicators for GYS at present, are not entirely satisfactory and there is a need to find an indicator which will detect GYS in a wide range of environments. The disease has not been previously detected, probably because of its similarity to GFV and TRSV and its peculiarity in expressing symptoms only in some environments.

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A Survey for Nine Major Viruses of Grapevines in Tennessee Vineyards

Plant Health Progress ◽

10.1094/php-03-20-0018-rs ◽

2020 ◽

Vol 21 (3) ◽

pp. 157-161

Author(s):

Nourolah Soltani ◽

Rongbin Hu ◽

Darrell D. Hensley ◽

David L. Lockwood ◽

Keith. L. Perry ◽

...

Keyword(s):

First Record ◽

Ringspot Virus ◽

Rt Pcr ◽

Grapevine Fanleaf Virus ◽

Arabis Mosaic Virus ◽

Growing Seasons ◽

Tomato Ringspot Virus ◽

The Status ◽

Diagnostic Approaches ◽

Das Elisa

Despite the significance of grape production to the fruit industry in Tennessee (TN), no published information has been available on viruses affecting grapevines in the state. Hence, a survey was conducted during the 2016 and 2017 growing seasons to determine the status of nine major viruses of grapevines in TN vineyards by taking advantage of classical serological assays and confirmatory nucleic acid-based diagnostic approaches. A total of 349 samples from 23 grapevine cultivars mostly displaying viral-like symptoms were collected from 23 commercial vineyards. All samples were assayed by DAS-ELISA for arabis mosaic virus (ArMV), grapevine leafroll-associated virus (GLRaV)-1, GLRaV-2, GLRaV-3, GLRaV-4, grapevine fanleaf virus (GFLV), tobacco ringspot virus (TRSV), and tomato ringspot virus (ToRSV). Selected serologically positive samples were also tested by RT-PCR, followed by Sanger sequencing of the generated amplicons. Additionally, 19 grapevines displaying symptoms characteristic of grapevine red blotch virus (GRBV) were also assayed by PCR followed by confirmatory sequencing-based methods. Collectively, these assays verifiably detected GLRaV-1, GLRaV-2, GLRaV-3, ToRSV, and GRBV in TN vineyards. This is the first record of the presence of these viruses in TN vineyards. ArMV, GLRaV-4, GFLV, and TRSV were not detected. The majority of samples tested positive for a single virus, whereas mixed infections with more than one virus were detected in 37% of samples.

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ANATOMICAL EFFECTS OF TOMATO RINGSPOT VIRUS IN VICIA FABA

American Journal of Botany ◽

10.1002/j.1537-2197.1957.tb10566.x ◽

1957 ◽

Vol 44 (6) ◽

pp. 470-477

Author(s):

Frank H. Smith ◽

Frank P. McWhorter

Keyword(s):

Vicia Faba ◽

Ringspot Virus ◽

Tomato Ringspot Virus

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