P.1.e.004 Sex-hormone fluctuation triggers subclinical depressive symptoms and affects immune cell numbers in peripheral blood in healthy women

2013 ◽  
Vol 23 ◽  
pp. S183-S184
Author(s):  
M. Heede ◽  
H. Søndergaard ◽  
A. Pinborg ◽  
K. Christensen ◽  
H. Ullum ◽  
...  
Keyword(s):  
Depressive Symptoms ◽  
Peripheral Blood ◽  
Immune Cell ◽  
Sex Hormone ◽  
Cell Numbers ◽  
Healthy Women

scholarly journals In-depth immune cellular profiling reveals sex-specific associations with frailty

2020 ◽  
Author(s):  
Leonard Daniël Samson ◽  
A. Mieke H. Boots ◽  
José A. Ferreira ◽  
H. Susan J. Picavet ◽  
Lia G. H. De Rond ◽  
...  
Keyword(s):  
T Cells ◽  
Random Forest ◽  
Peripheral Blood ◽  
Immune Cell ◽  
Cell Subpopulation ◽  
Men And Women ◽  
Random Forest Analysis ◽  
Cell Numbers ◽  
Immune Cell Subpopulations ◽  
Cell Subpopulations

Abstract Background: With advancing age, the composition of leukocyte subpopulations in peripheral blood is known to change, but how this change differs between men and women and how it relates to frailty is poorly understood. Thus, our aim in this exploratory study was to investigate whether frailty is associated with changes in immune cell subpopulations and whether associations differ between men and women. Therefore, we performed in-depth immune cell phenotyping by enumerating a total of 37 subsets of T cells, B cells, NK cells, monocytes, and neutrophils in peripheral blood of 289 elderly people between 60-87 years of age. Associations between frailty and each immune cell subpopulation were tested separately in men and women and were adjusted for age and CMV serostatus. In addition, a random forest algorithm was used to predict a participant’s frailty score based on enumeration of immune cell subpopulations. Results: In an association study, frailty was observed to be associated with increases in numbers of neutrophils in both men and in women. Furthermore, sex-specific associations were found. Frailer women, but not men, showed higher numbers of total and CD16^-^ monocytes and lower numbers of CD56^+^ T cells. Overall, the accuracy of the predictions in the random forest analysis was low (9.2% explained variance in men and 12.2% in women). Yet, the random forest analysis confirmed all associations mentioned above, but did not confirm a possible negative association in women between frailty and late differentiated CD4^+^ TemRA cells. Moreover, the random forest analysis revealed additional relationships with frailty, with frailer men showing higher CD16^+^ monocyte and lower naïve B cell numbers. Other important variables for predicting frailty were plasmablast numbers in men and total T cell numbers in women. Conclusions: We report on observed associations of frailty with elevated myeloid cell numbers in men and women. In-depth immune cellular profiling also revealed sex-specific associations of frailty with several immune subpopulations. However, an expected positive association between frailty and memory T cells was not observed. We hope that our study will prompt further investigation into the immune mechanisms associated with the development of frailty in men and women.

Diurnal rhythms in peripheral blood immune cell numbers of domestic pigs

2018 ◽  
Vol 79 ◽  
pp. 11-20 ◽  
Author(s):  
Larissa C. Engert ◽  
Ulrike Weiler ◽  
Birgit Pfaffinger ◽  
Volker Stefanski ◽  
Sonja S. Schmucker
Keyword(s):  
Peripheral Blood ◽  
Immune Cell ◽  
Diurnal Rhythms ◽  
Domestic Pigs ◽  
Cell Numbers

Deviations of the peripheral blood and intrahepatic immune cell landscape between NAFLD patients and healthy controls

2020 ◽  
Vol 73 ◽  
pp. S667
Author(s):  
Tom Diedrich ◽  
Andreas Drolz ◽  
Ansgar Lohse ◽  
Johannes Kluwe ◽  
Julian Schulze Zur Wiesch
Keyword(s):  
Peripheral Blood ◽  
Immune Cell ◽  
Healthy Controls

scholarly journals 8P Immune cell subsets in peripheral blood are associated with primary resistance to immunotherapy as frontline treatment in NSCLC

2020 ◽  
Vol 31 ◽  
pp. S1419-S1420
Author(s):  
H. Arasanz ◽  
M. Zuazo ◽  
L. Chocarro ◽  
A.I. Bocanegra Gondan ◽  
M. Martínez-Aguillo ◽  
...  
Keyword(s):  
Peripheral Blood ◽  
Immune Cell ◽  
Primary Resistance ◽  
Immune Cell Subsets ◽  
Frontline Treatment

scholarly journals Erratum to: Content of HLA-G+ T Cells in the Peripheral Blood from Healthy Women and Breast Cancer Patients

2016 ◽  
Vol 160 (4) ◽  
pp. 592-592
Author(s):  
E. O. Ostapchuk ◽  
Yu. V. Perfil’eva ◽  
Sh. Zh. Talaeva ◽  
N. A. Omarbaeva ◽  
N. N. Belyaev
Keyword(s):  
Breast Cancer ◽  
T Cells ◽  
Cancer Patients ◽  
Peripheral Blood ◽  
Breast Cancer Patients ◽  
Healthy Women

scholarly journals Evidence for oestrogen sensitivity in perinatal depression: pharmacological sex hormone manipulation study

2018 ◽  
Vol 215 (3) ◽  
pp. 519-527 ◽  
Author(s):  
Divya Mehta ◽  
Monika Rex-Haffner ◽  
Helle Bach Søndergaard ◽  
Anja Pinborg ◽  
Elisabeth B. Binder ◽  
...  
Keyword(s):  
Gene Expression ◽  
Dna Methylation ◽  
Depressive Symptoms ◽  
Perinatal Depression ◽  
Sex Hormone ◽  
Sex Steroid ◽  
Mood Changes ◽  
Induced Mood ◽  
Pharmacological Model

BackgroundEnhanced sensitivity to oestrogen signalling may drive increased risk for depressive symptoms when exposed to peripartum sex-steroid hormone fluctuations.AimTesting if 116 pre-identified sex steroid-responsive transcripts that predicted perinatal depression (PND) translates to a pharmacological model of hormone-induced mood changes.MethodWe generated longitudinal, genome-wide gene-expression and DNA-methylation data from 60 women exposed to a gonadotrophin-releasing hormone agonist (GnRHa) or placebo. We used linear mixed-effect models to assess differences between baseline and follow-up for gene expression and DNA methylation in the biphasic ovarian response to GnRHa.ResultsOf the 116 PND-predictive transcripts, a significant (19%) overlap was observed with those differentially expressed post-GnRHa at both early and later follow-up, indicating sustained effects. Similarly, 49% of tested genes were differentially methylated post-GnRHa at the late follow-up. Within the GnRHa group, a large proportion of PND genes were significantly associated (gene expression; DNA methylation) with changes in depressive symptoms (28%; 66%), oestradiol levels (49%; 66%) and neocortex serotonin transporter binding (8%; 45%) between baseline and follow-up.ConclusionsOur data bridge clinical PND biomarkers with a pharmacological model of sex hormone-induced mood changes and directly relate oestrogen-induced biological changes with depressive symptoms and associated serotonin-signalling changes. Our data highlight that individual variations in molecular sensitivity to oestrogen associate with susceptibility to hormone-induced mood changes and hold promise for candidate biomarkers.Declaration of interestV.G.F. received honorarium for being a speaker for H. Lundbeck A/S. E.B.B. receives research funding from Böhringer Ingelheim to investigate FKBP5 as a potential drug target for depression.

scholarly journals MicroRNA expression profiling of peripheral blood mononuclear cells associated with syphilis

2019 ◽  
Author(s):  
Tao Huang ◽  
Jun Zhang ◽  
Wujian Ke ◽  
Xiaohui Zhang ◽  
Wentao Chen ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Cell Function ◽  
Immune Cell ◽  
Treponema Pallidum ◽  
Target Sequence ◽  
Healthy Individuals ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

Abstract Background Treponema pallidum ( T. pallidum ) infection evokes significant immune responses, resulting in tissue damage. The immune mechanism underlying T. pallidum infection is still unclear, although microRNAs (miRNAs) have been shown to influence immune cell function and, consequently, the generation of antibody responses during other microbe infections. However, these mechanisms are unknown for T. pallidum . Methods In this study, we performed a comprehensive analysis of differentially expressed miRNAs in healthy individuals, untreated patients with syphilis, patients in the serofast state, and serologically cured patients. miRNAs were profiled from the peripheral blood of patients obtained at the time of serological diagnosis. Then, both the target sequence analysis of these different miRNAs and pathway analysis were performed to identify important immune and cell signaling pathways. Quantitative reverse transcription-polymerase chain reaction (RT-PCR) was performed for microRNA analysis. Results A total of 89 differentially regulated miRNAs were identified. Following RT-qPCR confirmation, three miRNAs (hsa-miR-195-5p, hsa-miR-223-3p, hsa-miR-589-3p) showed significant differences in the serofast and serologically cured states ( P <0.05). One miRNA (hsa-miR-195-5p) showed significant differences between untreated patients and healthy individuals. Conclusions This is the first study of miRNA expression differences in peripheral blood mononuclear cells (PBMCs) in different stages of T. pallium infection. Our study suggests that the combination of three miRNAs has great potential to serve as a non-invasive biomarker of T. pallium infections, which will facilitate better diagnosis and treatment of T. pallium infections.

Automated washing of long-term cryopreserved peripheral blood stem cells promotes cell viability and preserves CD34+ cell numbers

2018 ◽  
Vol 53 (9) ◽  
pp. 1225-1227
Author(s):  
Fatima Aerts-Kaya ◽  
Gül Koca ◽  
Parisa Sharafi ◽  
Funda Çakmak Sayla ◽  
Duygu Uçkan-Çetinkaya ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Viability ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Cell Numbers ◽  
Blood Stem Cells

Abstract 464: Chemokine Receptor CCR6 Expression on B Cells Augments Local IgM Production and Atheroprotection

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Prasad Srikakulapu ◽  
Chantel McSkimming ◽  
Coleen McNamara
Keyword(s):  
B Cells ◽  
B Cell ◽  
Adoptive Transfer ◽  
Chemokine Receptors ◽  
Immune Cell ◽  
Dependent Manner ◽  
Perivascular Adipose Tissue ◽  
Cell Recruitment ◽  
Cell Numbers ◽  
Atherosclerosis Development

Background: CCR6 mediates immune cell recruitment to inflammatory sites and has cell type-specific effects on diet-induced atherosclerosis in mice. Recent studies implicate the local immune responses in the adventitia/perivascular adipose tissue (PVAT) in atherosclerosis development. We have previously demonstrated that adoptive transfer of CD43 - splenocytes (B cells) into B cell deficient μMT -/- ApoE -/- mice results in reduced diet-induced atherosclerosis in a CCR6-dependent manner. Notably, there were significantly greater numbers of B cells in the aorta including PVAT of μMT -/- ApoE -/- mice which received splenic B cells from CCR6 +/+ mice compared to CCR6 -/- mice, despite no difference in B cell numbers in blood, spleen and peritoneal cavity, suggesting that CCR6 expression on B cells is important in B cell aortic homing. Production of IgM antibodies is thought to be a major mechanism whereby B cells limit atherosclerosis development. Yet whether B cells produce IgM locally in the PVAT and whether this is regulated by chemokine receptors such as CCR6 is unknown. Methods and Results: FACS experiments demonstrated high numbers of B cells available in the PVAT than aorta of young ApoE -/- (49121±11190 and 80±11; p<0.001, n=7) mice. ELISPOT experiments demonstrated significantly fewer IgM secreting cells were in the PVAT of ApoE -/- CCR6 -/- mice compared to ApoE -/- CCR6 +/+ mice (100±25 vs 850±150, p<0.05, n=5), despite no differences in IgM secreting cell numbers in spleen and bone marrow. Adoptive transfer of CD43 - splenic B cells from ApoE -/- CCR6 -/- and ApoE -/- CCR6 +/+ mice into secretory IgM deficient ApoE -/- sIgM -/- mice demonstrated significantly reduced atherosclerosis in mice that received B cells from ApoE -/- CCR6 +/+ mice compared to those that received B cells from ApoE -/- CCR6 -/- mice. Moreover, the B cells from ApoE -/- CCR6 +/+ mice attenuated atherosclerosis only when they were capable of secreting IgM. FACS data from human blood demonstrated that circulating B and T cells but not monocytes express CCR6, suggesting potential human relevance to our murine findings. Conclusion: Results provide evidence that CCR6 expression on B cells mediates B cell recruitment into aorta and/or PVAT to provide atheroprotection via IgM secretion.

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