The thermostability of an α-helical coiled-coil protein and its potential use in sensor applications

2001 ◽  
Vol 16 (9-12) ◽  
pp. 1051-1057 ◽  
Author(s):  
Rajesh R Naik ◽  
Sean M Kirkpatrick ◽  
Morley O Stone
Keyword(s):  
Coiled Coil ◽  
Sensor Applications ◽  
Potential Use

scholarly journals Targeted anion transporter delivery by coiled-coil driven membrane fusion

2016 ◽  
Vol 7 (3) ◽  
pp. 1768-1772 ◽  
Author(s):  
Nestor Lopez Mora ◽  
Azadeh Bahreman ◽  
Hennie Valkenier ◽  
Hongyu Li ◽  
Thomas H. Sharp ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Biomedical Research ◽  
Coiled Coil ◽  
Research Tool ◽  
Anion Transporters ◽  
Anion Transporter ◽  
Potential Use

Membrane fusion was used to deliver lipophilic synthetic anion transporters to membranes of GUVs and cells, bringing the potential use as a therapeutic or biomedical research tool closer.

scholarly journals Silicon Based Coplanar Capacitive Device for Liquid Sensor Applications

Sensors ◽  
2021 ◽  
Vol 21 (17) ◽  
pp. 5958
Author(s):  
Andrea G. Martinez-Lopez ◽  
David E. Guzmán-Caballero ◽  
Israel Mejia ◽  
Julio C. Tinoco
Keyword(s):  
Silicon Substrate ◽  
Capacitive Sensor ◽  
Smart Sensor ◽  
Viable Option ◽  
Sensor Applications ◽  
Field Coupling ◽  
Sensor Devices ◽  
Capacitance Model ◽  
Silicon Based ◽  
Potential Use

The development of silicon-based sensor devices has enabled the possibility to pursue novel integrated smart sensor technologies. Under this scenario, capacitive sensor devices are one viable option for implementing different kinds of applications. In this paper, an interdigitated coplanar capacitive device fabricated over a silicon substrate is presented and its potential use as liquid sensor is demonstrated. Additionally, a detailed capacitance model, which includes the parasitic capacitances introduced by the silicon substrate, was developed. The capacitance model has been theoretically validated through finite-element simulations as well as experimentally by comparison with fabricated devices. A polydimethylsiloxane mold has been fabricated and bonded to the sensor device with the aim of defining a cavity to collect the liquid sample into the device’s active region. The active capacitance component correlates to the electric field coupling between adjacent metal lines. Therefore, any change to the dielectric constant of the medium above the coplanar metal lines will produce a change to the device capacitance. Finally, the main guidelines for device performance improvement are depicted.

Study of magnetic behavior in co-precipitated Ni–Zn ferrite nanoparticles and their potential use for gas sensor applications

2020 ◽  
Vol 502 ◽  
pp. 166534 ◽  
Author(s):  
Ch. Srinivas ◽  
E. Ranjith Kumar ◽  
B.V. Tirupanyam ◽  
Sher Singh Meena ◽  
Pramod Bhatt ◽  
...  
Keyword(s):  
Gas Sensor ◽  
Magnetic Behavior ◽  
Ferrite Nanoparticles ◽  
Sensor Applications ◽  
Zn Ferrite ◽  
Potential Use

scholarly journals Loss of TACC1 variant25 inducing cell proliferation and suppressing autophagy in head and neck squamous carcinoma

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Pan Xu ◽  
Ran Zhao ◽  
Chen-Yang Zhang ◽  
Qian-Qian Zhang ◽  
Yong Wang ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Head And Neck ◽  
Cancer Biology ◽  
Coiled Coil ◽  
Squamous Carcinoma ◽  
Biological Functions ◽  
Fadu Cells ◽  
First Time ◽  
Multiple Variants ◽  
Potential Use

AbstractTransforming acidic coiled-coil containing protein1 (TACC1) is closely related to transcription, translation and centrosome dynamics. Dysregulation of TACC1 is associated with multiple malignancies. Alternative splicing (AS) of TACC1 produces multiple variants, which are of great significance in cancer biology. However, the expression and biological functions of TACC1 variants in head and neck squamous cell carcinoma (HNSCC) remain unclear. In this study, we found for the first time that TACC1 variants exhibited a characteristic expression pattern and that TACC1 variant25 (TACC1v25) was downregulated in HNSCC tissues and cell lines. Overexpression of TACC1v25 in Cal27 and Fadu cells significantly inhibited proliferation and promoted autophagy. Moreover, expression levels of nuclear pERK and p-mTOR were significantly decreased, while the expression of Beclin-1 and the LC3II/LC3I ratio were increased in TACC1v25-overexpressed Cal27 and Fadu cells. After the addition of AKT activator SC79 to TACC1v25-overexpressed Cal27 and Fadu cells, the autophagy levels were remarkably rescued. In conclusion, TACC1v25 inhibits HNSCC progression through the ERK and AKT/mTOR pathways by inhibiting proliferation and increasing autophagy. TACC1v25 might have potential use as a tumour suppressor in HNSCC.

The growth of mica polytypes as studied by conventional and high-resolution TEM

1983 ◽  
Vol 41 ◽  
pp. 174-177
Author(s):  
A. Baronnet ◽  
M. Amouric
Keyword(s):  
Water Pressure ◽  
Point Of View ◽  
Temperature Fields ◽  
Natural Occurrence ◽  
Growth Mechanisms ◽  
Long Period ◽  
High Resolution Tem ◽  
Layer Stacking ◽  
Experimental Works ◽  
Potential Use

The origin of mica polytypes has long been a challenging problem for crystal- lographers, mineralogists and petrologists. From the petrological point of view, interest in this field arose from the potential use of layer stacking data to furnish further informations about equilibrium and/or kinetic conditions prevailing during the crystallization of the widespread mica-bearing rocks. From the compilation of previous experimental works dealing with the occurrence domains of the various mica "polymorphs" (1Mr, 1M, 2M1, 2M2 and 3T) within water-pressure vs temperature fields, it became clear that most of these modifications should be considered as metastable for a fixed mica species. Furthermore, the natural occurrence of long-period (or complex) polytypes could not be accounted for by phase considerations. This highlighted the need of a more detailed kinetic approach of the problem and, in particular, of the role growth mechanisms of basal faces could play in this crystallographic phenomenon.

An atomic-resolution microscope study of Al contracts on GaAs

1986 ◽  
Vol 44 ◽  
pp. 726-727
Author(s):  
Z. Liliental-Weber ◽  
C. Nelson ◽  
R. Ludeke ◽  
R. Gronsky ◽  
J. Washburn
Keyword(s):  
High Speed ◽  
Schottky Contacts ◽  
Detailed Knowledge ◽  
The Past ◽  
Semiconductor Interfaces ◽  
Deposited Metal ◽  
Microwave Applications ◽  
Free Interfaces ◽  
Potential Use

The properties of metal/semiconductor interfaces have received considerable attention over the past few years, and the Al/GaAs system is of special interest because of its potential use in high-speed logic integrated optics, and microwave applications. For such materials a detailed knowledge of the geometric and electronic structure of the interface is fundamental to an understanding of the electrical properties of the contact. It is well known that the properties of Schottky contacts are established within a few atomic layers of the deposited metal. Therefore surface contamination can play a significant role. A method for fabricating contamination-free interfaces is absolutely necessary for reproducible properties, and molecularbeam epitaxy (MBE) offers such advantages for in-situ metal deposition under UHV conditions

TMCC3 localizes at the three-way junctions for the proper tubular network of the endoplasmic reticulum

2019 ◽  
Vol 476 (21) ◽  
pp. 3241-3260
Author(s):  
Sindhu Wisesa ◽  
Yasunori Yamamoto ◽  
Toshiaki Sakisaka
Keyword(s):  
Endoplasmic Reticulum ◽  
Membrane Proteins ◽  
Membrane Protein ◽  
Mammalian Cells ◽  
Coiled Coil ◽  
Transmembrane Domains ◽  
Domain Family ◽  
Coiled Coil Domain ◽  
U2os Cells ◽  
Er Membrane

The tubular network of the endoplasmic reticulum (ER) is formed by connecting ER tubules through three-way junctions. Two classes of the conserved ER membrane proteins, atlastins and lunapark, have been shown to reside at the three-way junctions so far and be involved in the generation and stabilization of the three-way junctions. In this study, we report TMCC3 (transmembrane and coiled-coil domain family 3), a member of the TEX28 family, as another ER membrane protein that resides at the three-way junctions in mammalian cells. When the TEX28 family members were transfected into U2OS cells, TMCC3 specifically localized at the three-way junctions in the peripheral ER. TMCC3 bound to atlastins through the C-terminal transmembrane domains. A TMCC3 mutant lacking the N-terminal coiled-coil domain abolished localization to the three-way junctions, suggesting that TMCC3 localized independently of binding to atlastins. TMCC3 knockdown caused a decrease in the number of three-way junctions and expansion of ER sheets, leading to a reduction of the tubular ER network in U2OS cells. The TMCC3 knockdown phenotype was partially rescued by the overexpression of atlastin-2, suggesting that TMCC3 knockdown would decrease the activity of atlastins. These results indicate that TMCC3 localizes at the three-way junctions for the proper tubular ER network.

The potential use of short chain peptides in parenteral nutrition

1985 ◽  
Vol 4 ◽  
pp. 116-123 ◽  
Author(s):  
P STEHLE ◽  
S ALBERS ◽  
I AMBERGER ◽  
P PFAENDER ◽  
P FURST
Keyword(s):  
Parenteral Nutrition ◽  
Short Chain ◽  
Potential Use

Instant 99mTc Compounds

1971 ◽  
Vol 10 (03) ◽  
pp. 245-251 ◽  
Author(s):  
P. Richards ◽  
W. C. Eckelman
Keyword(s):  
Physical Properties ◽  
High Purity ◽  
Analytical Techniques ◽  
Full Potential ◽  
Labeled Compounds ◽  
Ph Adjustment ◽  
One Step ◽  
Stannous Ion ◽  
99Mtc Radiopharmaceuticals ◽  
Potential Use

SummaryThe full potential use of technetium has not been achieved despite its ideal physical properties, dosimetry and availability because of the complex preparations required for 99mTc radiopharmaceuticals. One of the goals of our work is to develop techniques for the preparation of high-purity 99mTc compounds which can be easily prepared, ideally by adding pertechnetate to a prepared solution.The use of stannous ion as reducing agent for technetium makes it possible to obtain such one-step, high-purity products. All non-radioactive components can be premixed in a single vial before addition of the radioactive pertechnetate. No final pH adjustment, further chemical manipulation or purification is required.Procedures for two instantly labeled compounds have been developed to date: 99mTc DTPA and 99mTc HSA. The 99mTc DTPA is prepared by adding pertechnetate to a previously prepared solution of stannous ion and CaNa3 DTPA which has been stored at pH 4. The 99mTc HSA is prepared by adding pertechnetate to a solution of stannous ion and HSA. The parametric variations and analytical techniques involved in formulating these procedures are described. It appears that development of kits for other biologically interesting compounds may be possible using similar procedures.

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