213: The direct cytotoxic and P-glycoprotein modulating effects of Thai indigenous plant extracts in drug resistant HepG2 cells

The area of phytosynthesized nanomaterials is rapidly developing, with numerous studies being published yearly. The use of plant extracts is an alternative method to reduce the toxic potential of the nanomaterials and the interest in obtaining phytosynthesized nanoparticles is usually directed towards accessible and common plant species, ferns not being explored to their real potential in this field. The developed nanoparticles could benefit from their superior antimicrobial and antioxidant properties (compared with the nanoparticles obtained by other routes), thus proposing an important alternative against health care-associated and drug-resistant infections, as well as in other types of applications. The present review aims to summarize the explored application of ferns in nanotechnology and related areas, as well as the current bottlenecks and future perspectives, as emerging from the literature data.

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Adulticidal and repellent properties of indigenous plant extracts against Culex quinquefasciatus and Aedes aegypti (Diptera: Culicidae)

Parasitology Research ◽

10.1007/s00436-011-2669-9 ◽

2011 ◽

Vol 110 (5) ◽

pp. 1607-1620 ◽

Cited By ~ 47

Author(s):

Marimuthu Govindarajan ◽

Rajamohan Sivakumar

Keyword(s):

Aedes Aegypti ◽

Plant Extracts ◽

Culex Quinquefasciatus ◽

Indigenous Plant

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Evaluation of Some Indigenous Plant Extracts for Suppressing Bipolaris sorokiniana in Wheat Seed

The Agriculturists ◽

10.3329/agric.v7i1.5587 ◽

2010 ◽

pp. 96-104

Author(s):

Rokshana Panna ◽

FM Aminuzzaman ◽

MR Islam ◽

MHM Borhannuddin Bhuyan

Keyword(s):

Seed Germination ◽

Plant Extracts ◽

Leaf Extract ◽

Bipolaris Sorokiniana ◽

Leaf Extracts ◽

Pathology Laboratory ◽

Black Cumin ◽

Seed Pathology ◽

Indigenous Plant ◽

Black Cumin Seed

Studies were conducted in the Seed Pathology Laboratory, Department of Plant Pathology, Sher-e-Bangla Agricultural University to evaluate some indigenous plant extracts on the incidence of Bipolaris sorokiniana of wheat and seed germination. Seed treated with plant extracts reduced the incidence of Bipolaris sorokiniana. The lowest incidence was counted (12.33%) in seeds treated with Burmuda grass extracts (1:2W/V). While the highest seed germination (99.60%) was recorded in seeds treated with this grass extract followed by 99.33% germination in seeds treated with Neem leaf extract (1:2W/V), 98.00% with nut sedge grass rhizome extract (1:2 W/V), 87.67% with Allamanda leaf extract (1:2W/V). Comparatively the lowest incidence of Bipolaris sorokiniana (14.00%) was observed in seeds treated with Neem leaf extracts (1:2W/V) followed by 14.50% in seeds treated with Nut sedge grass rhizome extracts (1:2W/V), Black cumin seed extracts (15.20%) and Allamanda leaf extract (24.00%).

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Oviposition-deterrent, ovicidal, and repellent activities of indigenous plant extracts against Anopheles subpictus Grassi (Diptera: Culicidae)

Parasitology Research ◽

10.1007/s00436-009-1593-8 ◽

2009 ◽

Vol 105 (6) ◽

pp. 1567-1576 ◽

Cited By ~ 58

Author(s):

G. Elango ◽

A. Bagavan ◽

C. Kamaraj ◽

A. Abduz Zahir ◽

A. Abdul Rahuman

Keyword(s):

Plant Extracts ◽

Oviposition Deterrent ◽

Anopheles Subpictus ◽

Indigenous Plant

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The Ribosomal Protein L28 Gene Is Involved in Sorafenib Resistance in Hepatocellular Carcinoma

10.21203/rs.3.rs-259580/v1 ◽

2021 ◽

Author(s):

Yi Shi ◽

Xiaojiang Wang ◽

Qiong Zhu ◽

Gang Chen

Keyword(s):

Hepatocellular Carcinoma ◽

Drug Resistance ◽

Hepg2 Cells ◽

Cell Model ◽

Resistant Cell ◽

Drug Resistant ◽

Resistant Gene ◽

Key Genes ◽

Resistant Cells

Abstract Background: Sorafenib is the first molecular-targeted drug for the treatment of advanced hepatocellular carcinoma (HCC). However, its treatment efficiency decreases after a short period of time because of the development of drug resistance. This study investigates the role of key genes in regulating sorafenib-resistance in hepatocellular carcinoma and elucidates the mechanism of drug resistance. Methods: The HCC HepG2 cells were used to generate a sorafenib-resistant cell model by culturing the cells in gradually increasing concentration of sorafenib. RNA microarray was applied to profile gene expression and screen key genes associated with sorafenib resistance. Specific targets were knockdown in sorafenib-resistant HepG2 cells for functional studies. The HCC model was established in ACI rats using Morris hepatoma3924A cells to validate selected genes associated with sorafenib resistance in vivo. Results: The HepG2 sorafenib-resistant cell model was successfully established. The IC50 of sorafenib was 9.988mM in HepG2 sorafenib-resistant cells. A total of 35 up-regulated genes were detected by expression profile chip. High-content screening technology was used and a potential drug-resistant gene RPL28 was filtered out. After knocking down of RPL28 in HepG2 sorafenib-resistant cells, the results of cell proliferation and apoptosis illustrated that RPL28 is the key drug-resistant gene in the cells. Furthermore, it was found that both RNA and protein expression of RPL28 increased in HepG2 sorafenib-resistant specimens of Morris Hepatoma rats. In addition, the expression of functional proteins Ki-67 increased in sorafenib-resistant cells. Conclusion: Our study suggested that RPL28 was a key gene for sorafenib resistance in HCC both in vitro and in vivo.

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