Aqueous extract of Ocimum gratissimum Linn and ascorbic acid ameliorate nicotine–induced cellular damage in murine peritoneal macrophage

Abstract Background To investigate the antinociceptive and anti-inflammatory activities of aqueous extract of a blended mixture of dried leaves of Ocimum gratissimum and Psidium guajava, a traditional analgesic drug polyherbal (TADP) used as a remedy for pain-related conditions. Methods Antinociceptive activity of TADP (100, 200 and 400 mg/kg) was evaluated in the hot plate test and acetic acid-induced nociception in mice while the anti-inflammatory was evaluated in carrageenan-induced paw oedema in rats. Levels of nitrite, myeloperoxidase, glutathione and malondialdehyde were assayed in carrageenan-induced paw tissue. Results TADP (200 and 400 mg/kg) significantly prolong the latency time in the hot-plate test. TADP (100–400 mg/kg) produced a dose-dependent significant inhibition of the acetic-acid induced abdominal constriction. The antinociceptive activity of TADP in the presence of naloxone and atropine was not reversed whereas yohimbine and glibenclamide significantly reversed it. TADP (100, 200 and 400 mg/kg) significantly reduced the swelling in the carrageenan-induced oedema model and also produced a reduction in the nitrite and myeloperoxidase level. TADP (400 mg/kg) significantly reduced malondialdehyde concentration and increase glutathione level in the carrageenan-induced rat paw. TADP significantly decrease the number of cellular infiltrates in the histopathological assessment. Conclusion These results indicate that polyherbal product containing blended leaves of Ocimum gratissimum and Psidium guajava possess antinociceptive and anti-inflammatory properties, hence represents a promising alternative remedy in inflammation-induced pain.

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Physiological Response of Capsicum annuum L. to Aqueous Extracts of Allelopathic Plants: A Case of Tithonia rotundifolia and Murraya koenigii

International Journal of Plant & Soil Science ◽

10.9734/ijpss/2019/v31i130199 ◽

2019 ◽

pp. 1-10

Author(s):

O. O. Otusanya ◽

A. A. Ogunwole ◽

M. O. Tijani

Keyword(s):

Ascorbic Acid ◽

Leaf Area ◽

Capsicum Annuum ◽

Aqueous Extract ◽

Crude Protein ◽

Dry Weight ◽

Protein Accumulation ◽

Aqueous Extracts ◽

Total Chlorophyll ◽

Murraya Koenigii

The present study evaluated the allelopathic potential of fresh shoot aqueous extracts of Tithonia rotundifolia (FSET) and Murraya koenigii (FSEM) on the germination of seeds, growth, chlorophyll, ascorbic acid and percentage crude protein accumulation of Capsicum annuum. The laboratory experimental results showed that seed germination and juvenile seedling growth of C. annuum were significantly retarded by both FSEM and FSET. The radicle growth was more susceptible to the phytotoxicity of both extracts than the plumule growth. Both plants extract had a concentration-dependent inhibitory effect on the seedling growth of the recipient crop while the FSET was more phytotoxic than FSEM. However, this retardatory effect of aqueous extracts on growth observed in the laboratory was reversed in the soil-cultured experiments such that application of FSEM significantly enhanced all the studied growth parameters (shoot height, number of leaves, leaf area, leaf area ratio, shoot fresh and dry weight, root fresh and dry weight, chlorophyll a, chlorophyll b, total chlorophyll, ascorbic acid content in the shoot and percentage crude protein in the shoot and fruits). Likewise, FSET application significantly increased the leaf area, total chlorophyll and percentage crude protein accumulation in the shoot and fruits of the recipient crop at P<.05. This study then emphasizes the fact that T. rotundifolia and M. koenigii are allelopathic plants. The increased ascorbic acid and percentage crude protein accumulation in the aqueous extract-treated crop could, apart from enhancing the nutritional benefits of the test crop, be an adaptive mechanism evolved by the crop to overcome the allelopathic stress posed by the application of the aqueous extracts. These findings therefore suggest that allelochemicals in the aqueous extract of M. koenigii and T. rotundifolia could serve as biofertilizers for boosting the production of C. annuum.

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Antioxidant Effects of Aqueous Extract of Sweet Potato and Ascorbic Acid on Paracetamol Induced Damage

Journal of Medical Sciences(Faisalabad) ◽

10.3923/jms.2006.87.92 ◽

2005 ◽

Vol 6 (1) ◽

pp. 87-92

Author(s):

A.O. Lawal . ◽

A. Ologundudu . ◽

F.O. Obi . ◽

I.V. Ogungbe . ◽

A.O. Olakanye . ◽

...

Keyword(s):

Ascorbic Acid ◽

Sweet Potato ◽

Aqueous Extract ◽

Antioxidant Effects

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Effects of aqueous extract of ocimum gratissimum and momordica charantia on learning and memory in scopolamine-induced dementia rats model

IBRO Reports ◽

10.1016/j.ibror.2019.09.027 ◽

2019 ◽

Vol 7 ◽

pp. 11

Author(s):

M.T. Ayinla ◽

B.V. Owoyele ◽

W. Abdulmajeed ◽

A. Imam

Keyword(s):

Learning And Memory ◽

Aqueous Extract ◽

Momordica Charantia ◽

Ocimum Gratissimum

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EFFECT OF MANIHOT ESCULENTA AQUEOUS EXTRACT AND THERAPEUTIC ULTRASOUND IN ACCELERATING THE WOUND HEALING PROCESS IN VITRO

Jurnal Teknologi ◽

10.11113/jt.v81.13135 ◽

2019 ◽

Vol 81 (4) ◽

Author(s):

Ulfah Anwar ◽

Siti Pauliena Mohd Bohari

Keyword(s):

Wound Healing ◽

Ascorbic Acid ◽

Aqueous Extract ◽

Manihot Esculenta ◽

Wound Closure ◽

Healing Process ◽

Therapeutic Ultrasound ◽

Wound Healing Process ◽

Time Interval

The aim of this research is to investigate the wound healing process in in vitro by combining the Manihot esculenta aqueous extract and therapeutic ultrasound. Firstly, the optimization seeding densities of HSF cell 1184 in six-well plate, and then followed by the scratch assay experiment. The scratched that made was treated with the remedial treatments (Manihot esculenta aqueous extract only; ascorbic acid+ therapeutic ultrasound; Manihot esculenta aqueous extract+ ascorbic acid; Manihot esculenta aqueous extract+ therapeutic ultrasound and also the combination of these three materials). The rate of wound closure was observed and analysed at a time interval of 0, 2, 4, 6, 8, 10 and 24 h by using image J software. Then, the cells viability were analysed using the MTT assay. The result showed that Manihot esculenta aqueous extract coupled with specific dose therapeutic ultrasound represents a significantly high rate of wound closure at 96.10 % with the cell numbers at 5.44×105 cells/mL when compared to the other combination therapy. The finding of this study revealed that Manihot esculenta aqueous extract 200 µg/mL and the therapeutic ultrasound specific dose (3 MHz, 300 mWatt/cm2, 50% in 5 min) have the potential in accelerating wound healing process of cells in in vitro.

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