Effect of Piper betel leaf stalk extract on protein metabolism in reproductive tissues of male albino rats

Alzheimer's disease is a common neurological disorder affecting a significant proportion of the elderly population.There are only a few drugs which can be used safely to treat it. We sought to investigate for the first time Piper betel leaf, a postmeal mouth freshener for its potential use in Alzheimer's disease. Five groups of male Wistar rats with six rats in each group aged 10-12 weeks were used. The control group received the distilled water, aluminium chloride (AlCl3) group was treated with AlCl3, the standard group was treated with rivastigmine with AlCl3, and the two test groups received piper betel leaf extract (PBE) at doses of 400mg/kg and 500mg/kg body weight with AlCl3. AlCl3 was administered orally for 42 days in all the treated groups. Memory and learning were evaluated by Morris water maze test and Passive avoidance test.The results of the Morris water maze test showed reduced mean escape latency period in all the groups on trial day three (P≤0.05) and on trial day four (P ≤0.01) compared to AlCl3 group. The retention of spatial memory by probe trial showed that PBE and rivastigmine treated group spent more time in the target (platform) quadrant when compared to AlCl3 group (P≤0.01). The passive avoidance test showed a significant increase in step through latency in standard and test groups compared to the AlCl3 treated group. The weight of the rats treated with AlCl3 and PBE was reduced at the end of the treatment period while increased in standard and control group. The study shows the beneficial effects of Piper betel leaves in Alzheimer’s disease by significantly improving the learning and memory functions in rats.

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Efektivitas Perlakuan Kombinatif Plasma Medis dan Ekstrak Daun Sirih untuk Mempercepat Penyembuhan Luka Fase Proliferasi pada Model Mencit Diabetik

Jurnal Ilmiah Kesehatan Keperawatan ◽

10.26753/jikk.v15i2.378 ◽

2019 ◽

Vol 15 (2) ◽

pp. 81

Author(s):

Eka Sakti Wahyuningtyas ◽

Nasruddin Nasruddin ◽

Heni Setyowati Esti Rahayu ◽

Heni Lutfiyati ◽

Isabella Meliawati Sikumbang ◽

...

Keyword(s):

Wound Healing ◽

Leaf Extract ◽

Plasma Jet ◽

Diabetic Wound ◽

Diabetic Mice ◽

Plasma Medicine ◽

Betel Leaf ◽

Diabetic Wound Healing ◽

Diabetic Wounds ◽

Piper Betel Leaf

The continued increase in the number of people with Diabetes Mellitus (DM) in Indonesia is a serious problem. One of the big problems for people with Diabetes Mellitus (DM) is the emergence of complications of diabetic wounds. To date the strategy for treatment of diabetic wounds has been limited to the use of wound dressing, cell therapy and oxygen therapy. The problem is that the strategy is not fully successful. Thus, it is very important to look for new strategies to improve the quality of diabetic wound healing, such as by applying a combination of plasma medicine and local natural product, like the extraction of Daun sirih (Piper betle) leaves. Plasma medicine is a relatively new and multidisciplinary study involving plasma science, biomedical, pharmaceutical and other health sciences aimed at applying plasma to therapeutic health. Plasma is the fourth phase of matter, after the solid, liquid and gas phase. The medical aspects of plasma are related to the ability of plasma to produce biological molecules Reactive Oxygen and Nitrogen Species (RONS). If RONS is controlled in the right dosage it can be efficacious for health therapy. This study intends to examine the effects of combinative treatment of plasma medicine and Piper betel leaf extract for proliferation phase of wound healing in diabetic small animal model. This study used male Balb c mice with acute wounds which were divided into 5 groups, namely groups of untreated normal mice (ND-TP), groups of untreated diabetic mice (D-TP), groups of diabetic mice wounds with Piper betel leaf extract (DS ), the wound group of diabetic mice with plasma medicine (DP) and the wound group of diabetic mice with plasma medicine and Piper betel leaf (DPS). The plasma medicine was treated on wound with condition non-contact style (the plasma jet did not touch the wound) with a distance of plasma jet reactor nozzle to the surface of wound about 20 mm, for 2 minutes, every day. Macroscopic observation of wounds is carried out every day from day 0 to 7. On day 7 it was seen that the size of the wound area for D-P-S was smaller than the other groups. The results of this study indicated that Piper betel leaf extract can potentially be used to optimize the performance of plasma medicine in accelerating diabetic wound healing during the proliferation phase. Further investigation, however, is important to be conducted to study the effect for all phases of wound healing and its mechanism histo-pathologically.

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INHIBITORY EFFECT OF RED PIPER BETEL LEAF ETHANOL EXTRACT (PIPER CROCATUM RUIZ AND PAV.) AGAINST TRICHOMONAS VAGINALIS TROPHOZOITES IN VITRO

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017.v10i11.20778 ◽

2017 ◽

Vol 10 (11) ◽

pp. 311

Author(s):

Sri Agung Fitri Kusuma ◽

Widyastuti S Manan ◽

Fajar Budiman

Keyword(s):

Trichomonas Vaginalis ◽

Ethanol Extract ◽

Inhibitory Effect ◽

Betel Leaf ◽

Extract Concentration ◽

Standard Procedures ◽

Vaginal Secretions ◽

Piper Betel Leaf

Objective: The objective of this study is to determine the minimum inhibitory concentration (MIC) of red betel leaf ethanol extract against Trichomonas vaginalis trophozoites.Materials and Methods: The samples of red piper betel leaf were extracted with 70% ethanol using a maceration method. The phytochemical screening of ethanol extract was performed using the standard procedures. Isolation of T. vaginalis trophozoites from vaginal secretions was conducted using swab method, then suspending it into a saline solution sterile and followed by observing trophozoites motility using a microscope. While the anti-leucorrhea activity test and determination of MIC extract was done using macrodillution method. The inhibitory effect of each extract concentration to trichomonas trophozoites’ morphology was observed under a microscope using staining techniques by adding Giemsa dyes.Results: The extract of red piper betel leaves contains several secondary metabolites such as alkaloids, flavonoids, polyphenols, quinones, and saponins. Of the six vaginal secretions, four positive trichomoniasis secretions were obtained. The results of anti-leucorrhea activity of red piper betel ethanol extract showed that the number of inactive trophozoites was proportional to the increasing of extract concentration. The inactive trophozoites were characterized by the absence of its motility under microscope observation. The MIC value of red piper betel extract of T. vaginalis trophozoites was 5% w/v.Conclusion: It can be concluded that the ethanol extract of red piper betel leaves could be contribute as a natural and safe anti-leucorrhea candidate for in vivo study on trichomoniasis in the future.

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Antispermatogenic Effect of Piper betel Leaf Stalk Extract with Reference to Kinetic Studies of 17β-hydroxy Steroid Dehydrogenase Enzyme in Testes of Albino Rats

Current Enzyme Inhibition ◽

10.2174/1573408014666181018113911 ◽

2019 ◽

Vol 15 (1) ◽

pp. 36-40

Author(s):

Govardhan Naik Amgoth ◽

P. Venkataramanaiah ◽

Changamma Challa

Keyword(s):

Reproductive Function ◽

Kinetic Studies ◽

Albino Rats ◽

Activity Levels ◽

Enzyme Active Site ◽

Betel Leaf ◽

Male Reproductive Function ◽

Km Value ◽

Hydroxy Steroid ◽

Steroid Dehydrogenase

Background: In the development and maintenance of male reproductive function and fertility, steroidogenesis plays a key role. Aims: The aim of the present study was to investigate the effect of the betel leaf stalk extract on 17β- hydroxy steroid dehydrogenase activity levels. Methods: The observed elevation in testicular cholesterol levels may be due to decreased androgen production, which resulted in impaired spermatogenesis. The decreased steroidogenic enzyme 17β- HSD activity represents decreased androgen production by the extract administration. Reduction in enzyme active site density and Km value revealed that there was a reduction in enzyme-substrate affinities and rate of E-S complex breakdown in the administered rat testes. Results & Conclusion: The administration of betel leaf stalk extract resulted in decreased enzyme content probably through impaired synthesis.

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