Pollen abortion and male sterility

1997 ◽  
pp. 120-151 ◽  
Keyword(s):  
Male Sterility ◽  
Pollen Abortion

scholarly journals Mitochondrial localization of ORF346 causes pollen abortion in alloplasmic male sterility

2021 ◽  
Author(s):  
Shifei Sang ◽  
Hongtao Cheng ◽  
Mengyu Hao ◽  
Bingli Ding ◽  
Desheng Mei ◽  
...  
Keyword(s):  
Male Sterility ◽  
Mitochondrial Localization ◽  
Pollen Abortion ◽  
Alloplasmic Male Sterility

scholarly journals Identification and Verification of Genes Related to Pollen Development and Male Sterility Induced by High Temperature in Thermo-sensitive Genic Male Sterile Wheat Line YanZhan 4110S

2020 ◽  
Author(s):  
Xuetong Yang ◽  
Jiali Ye ◽  
Fuqiang Niu ◽  
Yi Feng ◽  
Xiyue Song
Keyword(s):  
Gene Silencing ◽  
Male Sterility ◽  
Mosaic Virus ◽  
Pollen Development ◽  
Bioinformatics Analysis ◽  
Virus Induced Gene Silencing ◽  
Wheat Line ◽  
Pollen Abortion ◽  
Hub Genes ◽  
Genic Male Sterility

Abstract Background: Environment-sensitive genic male sterility is of vital importance to hybrid vigor in crop production and breeding, therefore, it is meaningful to identify and study the function of the genes related to pollen development and male sterility, which still not fully understanding currently. In this study, Yanzhan 4110S, a new thermo-sensitive genic male sterility (TGMS) wheat line, and its near isogenic line Yanzhan 4110 were carried out cytological features observation, bioinformatics analysis to investgate the abortion state and identified the genes involved in pollen development which have fertility regulation function. Barely stripe mosaic virus-induced gene silencing was used to verify the genes function.Results: Cytological analysis showed pollen abortion event of Yanzhan 4110S occur at the later uninucleate stage (Lun) under higher temperature induction (day/night temperatures of 22 °C/20 °C), when the anthers were collected and assessed for transcriptomic profiling through high-throughput sequencing. We then in-depth analyzed the differentially expressed genes (DEGs) by Gene Ontology, Kyoto Encyclopedia of Genes and Genomes, the results showed that the occurrence of Yanzhan 4110S male-sterility most likely related to metabolic pathway, including phenylpropanoid biosynthesis in the biosynthesis of other secondary metabolites, starch and sucrose metabolism in carbohydrate metabolism, carbon fixation in photosynthetic organisms as well as carbon metabolism in energy metabolism. The weighted gene co-expression network analysis in the transcriptome profiles further identified some hub genes, where the key genes involved in those pathways were intersection between the unique DEGs of Yanzhan 4110S in anther and hub genes, totally 228 genes, which were highly related to pollen development including TaMut11 and TaSF3. Moreover, further verification through barely stripe mosaic virus-induced gene silencing elucidated that the silencing of TaMut11 and TaSF3 caused pollen abortion, finally resulting in the declination of fertility. So, the genes TaMut11 and TaSF3 are related to fertility conversion of Yanzhan 4110S.Conclusion: Through comparative transcriptome bioinformatics analysis, the genes TaMut11 and TaSF3 associated with pollen development and male sterility induced by high temperature were identified in Yanzhan 4110S, and verificated by barely stripe mosaic virus-induced gene silencing. These findings provided researching the abortive mechanism in environment-sensitive genic male sterility wheat.

scholarly journals Cytological observation of anther structure and genetic investigation of a thermo-sensitive genic male sterile line 373S in Brassica napus L

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Yanyan Sun ◽  
Dongsuo Zhang ◽  
Zhenzhen Wang ◽  
Yuan Guo ◽  
Xiaomin Sun ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Male Sterility ◽  
Spontaneous Mutation ◽  
Hybrid Breeding ◽  
Pcr Analysis ◽  
Cytological Observation ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Genetic Investigation ◽  
Temperature Changes

Abstract Background Photoperiod and/or thermo-sensitive male sterility is an effective pollination control system in crop two-line hybrid breeding. We previously discovered the spontaneous mutation of a partially male sterile plant and developed a thermo-sensitive genic male sterile (TGMS) line 373S in Brassica napus L. The present study characterized this TGMS line through cytological observation, photoperiod/ temperature treatments, and genetic investigation. Results Microscopic observation revealed that the condensed cytoplasm and irregular exine of microspores and the abnormal degradation of tapetum are related to pollen abortion. Different temperature and photoperiod treatments in field and growth cabinet conditions indicated that the fertility alteration of 373S was mainly caused by temperature changes. The effects of photoperiod and interaction between temperature and photoperiod were insignificant. The critical temperature leading to fertility alteration ranged from 10 °C (15 °C/5 °C) to 12 °C (17 °C/7 °C), and the temperature-responding stage was coincident with anther development from pollen mother cell formation to meiosis stages. Genetic analysis indicated that the TGMS trait in 373S was controlled by one pair of genes, with male sterility as the recessive. Multiplex PCR analysis revealed that the cytoplasm of 373S is pol type. Conclusions Our study suggested that the 373S line in B. napus has a novel thermo-sensitive gene Bnmst1 in Pol CMS cytoplasm background, and its fertility alteration is mainly caused by temperature changes. Our results will broaden the TGMS resources and lay the foundation for two-line hybrid breeding in B. napus.

scholarly journals The Major Factors Causing the Microspore Abortion of Genic Male Sterile Mutant NWMS1 in Wheat (Triticum aestivum L.)

2019 ◽  
Vol 20 (24) ◽  
pp. 6252 ◽  
Author(s):  
Junchang Li ◽  
Jing Zhang ◽  
Huijuan Li ◽  
Hao Niu ◽  
Qiaoqiao Xu ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Male Sterility ◽  
Genetic Research ◽  
Triticum Aestivum L ◽  
Anther Development ◽  
Sucrose Metabolism ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Microspore Stage ◽  
Regulation Model

Male sterility is a valuable trait for genetic research and production application of wheat (Triticum aestivum L.). NWMS1, a novel typical genic male sterility mutant, was obtained from Shengnong 1, mutagenized with ethyl methane sulfonate (EMS). Microstructure and ultrastructure observations of the anthers and microspores indicated that the pollen abortion of NWMS1 started at the early uninucleate microspore stage. Pollen grain collapse, plasmolysis, and absent starch grains were the three typical characteristics of the abnormal microspores. The anther transcriptomes of NWMS1 and its wild type Shengnong 1 were compared at the early anther development stage, pollen mother cell meiotic stage, and binucleate microspore stage. Several biological pathways clearly involved in abnormal anther development were identified, including protein processing in endoplasmic reticulum, starch and sucrose metabolism, lipid metabolism, and plant hormone signal transduction. There were 20 key genes involved in the abnormal anther development, screened out by weighted gene co-expression network analysis (WGCNA), including SKP1B, BIP5, KCS11, ADH3, BGLU6, and TIFY10B. The results indicated that the defect in starch and sucrose metabolism was the most important factor causing male sterility in NWMS1. Based on the experimental data, a primary molecular regulation model of abnormal anther and pollen developments in mutant NWMS1 was established. These results laid a solid foundation for further research on the molecular mechanism of wheat male sterility.

scholarly journals Morphological characteristics and transcriptome analysis at different anther development stages of the male sterile mutant MS7–2 in Wucai (Brassica campestris L.)

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Jian Wang ◽  
Yitao Yang ◽  
Lei Zhang ◽  
Shaoxing Wang ◽  
Lingyun Yuan ◽  
...  
Keyword(s):  
Male Sterility ◽  
Brassica Campestris ◽  
Morphological Characteristics ◽  
Development Stage ◽  
Phenylpropanoid Metabolism ◽  
Male Sterile ◽  
Pollen Abortion ◽  
Flower Buds ◽  
Flower Bud ◽  
Sterile Mutant

Abstract Background The discovery of male sterile materials is of great significance for the development of plant fertility research. Wucai (Brassica campestris L. ssp. chinensis var. rosularis Tsen) is a variety of non-heading Chinese cabbage. There are few studies on the male sterility of wucai, and the mechanism of male sterility is not clear. In this study, the male sterile mutant MS7–2 and the wild-type fertile plant MF7–2 were studied. Results Phenotypic characteristics and cytological analysis showed that MS7–2 abortion occurred at the tetrad period. The content of related sugars in the flower buds of MS7–2 was significantly lower than that of MF7–2, and a large amount of reactive oxygen species (ROS) was accumulated. Through transcriptome sequencing of MS7–2 and MF7–2 flower buds at three different developmental stages (a–c), 2865, 3847, and 4981 differentially expressed genes were identified in MS7–2 at the flower bud development stage, stage c, and stage e, respectively, compared with MF7–2. Many of these genes were enriched in carbohydrate metabolism, phenylpropanoid metabolism, and oxidative phosphorylation, and most of them were down-regulated in MS7–2. The down-regulation of genes involved in carbohydrate and secondary metabolite synthesis as well as the accumulation of ROS in MS7–2 led to pollen abortion in MS7–2. Conclusions This study helps elucidate the mechanism of anther abortion in wucai, providing a basis for further research on the molecular regulatory mechanisms of male sterility and the screening and cloning of key genes in wucai.

scholarly journals TaEXPB5 is responsible for male fertility in thermo-sensitive male-sterility wheat with Aegilops kotschyi cytoplasm

2021 ◽  
Author(s):  
Xingxia Geng ◽  
Xiaoxia Wang ◽  
Jingchen Wang ◽  
Xuetong Yang ◽  
lingli zhang ◽  
...  
Keyword(s):  
Male Sterility ◽  
Pollen Development ◽  
Male Fertility ◽  
Crop Production ◽  
Hybrid Vigor ◽  
Abnormal Development ◽  
Hybrid Breeding ◽  
Pollen Abortion ◽  
Gene Encoding ◽  
Early Degradation

Thermo-sensitive male sterility is of vital importance to heterosis, or hybrid vigor in crop production and hybrid breeding. Therefore, it is meaningful to study the function of the genes related to pollen development and male sterility, which is still not fully understand currently. Here, we conducted comparative analyses to screen fertility related genes using RNA-seq, iTRAQ, and PRM-based assay. A gene encoding expansin protein in wheat, TaEXPB5, was isolated in KTM3315A, which was in the cell wall and preferentially upregulated expression in the fertility anthers. The silencing of TaEXPB5 displayed pollen abortion, the declination or sterility of fertility. Further, cytological investigation indicated that the silencing of TaEXPB5 induced the early degradation of tapetum and abnormal development of pollen wall. These results revealed that the silencing of TaEXPB5 could eliminate the effects of temperature on male fertility, and resulting in functional loss of fertility conversion, which implied that TaEXPB5 may be essential for anther or pollen development and male fertility of KTM3315A. These findings provide a novel insight into molecular mechanism of fertility conversion for thermo-sensitive cytoplasmic male-sterility wheat, and contribute to the molecular breeding of hybrid wheat in the future.

Deviant DNA methylation play a key role in the pollen abortion of Gossypium barbadense L. cytoplasmic male sterility

2020 ◽  
Vol 154 ◽  
pp. 112622
Author(s):  
Xiangjun Kong ◽  
Aziz Khan ◽  
Bin Li ◽  
Jie Zheng ◽  
Farman Ullah Dawar ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Gossypium Barbadense ◽  
Pollen Abortion

scholarly journals Metabolomics Studies on Cytoplasmic Male Sterility during Flower Bud Development in Soybean

2019 ◽  
Vol 20 (12) ◽  
pp. 2869 ◽  
Author(s):  
Xianlong Ding ◽  
Xuan Wang ◽  
Qiang Li ◽  
Lifeng Yu ◽  
Qijian Song ◽  
...  
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Antioxidant Enzyme ◽  
Antioxidant Enzyme Activities ◽  
Ros Scavenging ◽  
Pollen Abortion ◽  
Flower Buds ◽  
Flower Bud ◽  
Cms Line ◽  
Metabolite Content

Abnormal reactive oxygen species (ROS) may mediate cytoplasmic male sterility (CMS). To observe the effect of ROS on soybean CMS, metabolite content and antioxidant enzyme activity in the flower buds between soybean N8855-derived CMS line and its maintainer were compared. Of the 612 metabolites identified, a total of 74 metabolites were significantly differentiated in flower buds between CMS line and its maintainer. The differential metabolites involved 32 differential flavonoids, 13 differential phenolamides, and 1 differential oxidized glutathione (GSSG) belonging to a non-enzymatic ROS scavenging system. We observed lower levels of flavonoids and antioxidant enzyme activities in flower buds of the CMS line than in its maintainer. Our results suggest that deficiencies of enzymatic and non-enzymatic ROS scavenging systems in soybean CMS line cannot eliminate ROS in anthers effectively, excessive accumulation of ROS triggered programmed cell death and ultimately resulted in pollen abortion of soybean CMS line.

Development Anatomy in Wheat of Male Sterility Induced by Heat Stress, Water Deficit or Abscisic Acid

1984 ◽  
Vol 11 (4) ◽  
pp. 243 ◽  
Author(s):  
HS Saini ◽  
M Sedgley ◽  
D Aspinall
Keyword(s):  
Abscisic Acid ◽  
Heat Stress ◽  
Water Stress ◽  
Male Sterility ◽  
Abnormal Development ◽  
Pollen Abortion ◽  
Developmental Anatomy ◽  
Partial Sterility ◽  
Type 3

Well watered wheat (Triticum aestivum L. cv. Gabo) plants grown at 20°C were subjected to heat stress (30°C for 3 days), water stress (leaf water potential -2.54 MPa) or exogenous application of abscisic acid (ABA, 3 × 10-5 M) during meiosis in the pollen mother cells. All these treatments caused male sterility. The developmental anatomy of abortive anthers and pollen grains was studied using light microscopy. The anatomical events leading to pollen abortion in response to heat stress, water stress, and abscisic acid were generally similar. Three developmental sequences leading to sterility were identified. Type 1 was observed in response to heat stress and ABA. Premature tapetal degeneration resulted in periplasmodial invasion of the locule at meiosis and resulted in total sterility. Type 2 was observed in response to water stress when the microspores lost contact with the tapetum at first pollen grain mitosis (PGM I) and the filament degenerated. This also resulted in total sterility. Type 3 was observed in response to all three stresses and led to partial sterility. The microspores completed PGM 1 but a proportion became disoriented from the tapetum and developed no further. Each stress treatment resulted in two groups of abnormal anther locules, one completely sterile and the other partially sterile. Heat stress and ABA resulted in very similar abnormal development which differed only in the proportion of the two types (1 and 3). Water stress produced partial sterility through the same developmental pattern as heat stress and ABA (type 3). The sequence leading to total sterility in water stress affected anthers (type 2) was similar to that resulting in partial sterility (type 3). The results do not support the hypothesis that ABA is the single primary controlling agent in water stress induced sterility and the question of whether similar mechanisms lead to male sterility in response to the three treatments remains unanswered.

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