scholarly journals 1. Traditional and Electronic Ki-67 Quantitation in Oligodendrogliomas

2015 ◽  
Vol 42 (S2) ◽  
pp. S2-S2
Author(s):  
S. Asiry ◽  
P. Rizek ◽  
R. Hammond
Keyword(s):  
Histological Grade ◽  
Prognostic Significance ◽  
Proliferative Index ◽  
Ki 67 ◽  
Proliferation Markers ◽  
Who Grade ◽  
The Central Nervous System ◽  
High Concordance ◽  
Gastroenteropancreatic Neuroendocrine Tumours ◽  
Automated Methods

The Ki-67 proliferative index has become a useful, objective, immunohistochemical tool that can aid in grading and prognostication for patients with oligodendrogliomas. Previous studies have described the prognostic significance of the Ki-67 index for such patients.According to the WHO classification of tumors of the central nervous system (2007) ”mitotic activity is low in WHO grade II oligodendroglioma, and labeling indices for proliferation markers are accordingly low, usually below 5%”. Furthermore, the predictive value of the Ki-67 index appears to be independent of age, tumor site, and histological grade. What is less well described is the relative accuracy of traditional vs. semi-automated methods of enumeration for a test where small differences can influence grading, prognosis and treatment. Tang et al. (2012), studying gastroenteropancreatic neuroendocrine tumours, found high concordance between two semi-automated methods for Ki-67 quantitation whereas “eyeballed estimates” were far less reliable. We will compare the reported proliferative index estimates to those calculated by digital image analysis of 35 recent oligodendrogliomas from the LHSC Pathology archives.

Predictive value of p53 and ki67 in different grades of meningioma

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Sarah ali Abed ◽  
Sabah Ahmed Abid Abu Sabe
Keyword(s):  
Histological Grade ◽  
Suppressor Gene ◽  
Malignant Meningioma ◽  
Ki 67 ◽  
Who Grade ◽  
Brain Invasion ◽  
The Central Nervous System ◽  
The Mean ◽  
Proliferative Marker ◽  
The Brain

Meningiomas are common tumors of the central nervous system that originate from the meningeal coverings of the brain and the spinal cord. Many factors are involved in tumor progression,one of the important factors is mutation of tumor suppressor gene p53. Ki-67 is considered to be the most reliable proliferative marker predicting tumor behavior.The aim of the present study is to evaluate the use ofimmunhistochemical expression of p53 and ki67 for predicting the grades of meningioma which are important in their prognosis.Sixty patients with different grades of meningioma were taken. Histological sections of the paraffin embedded tissues of these cases were be taken for H&E staining for assessment of histological grade according to WHO grading system. Immunohistochemical staining of sections of the paraffin embedded tissues by using monoclonal antibody for P53 & ki-67 were done. Correlation between tumor grades and immunhistochemical markers were done.The presences of mitosis,sheeting,prominent nucleoli,hypercellularity,pleomorphism,and the necrosis,except brain invasion,were correlated with tumor grades. The mean Ki-67 labeling index was significantly higher in meningiomas of WHO grade III than in those of grades I and II. Overexpression of p53 was found mainly in the atypical and malignant meningioma group. There is a good and positive correlation between Ki-67 and p53 expression.The Ki-67 proliferative marker and p53 are both presented highly in atypical and malignant meningioma,the routine use of them in predicting the behavior of meningiomas may be advocated based on the results of this study.

scholarly journals Prognostic Significance of Preoperative Neutrophil-to-Lymphocyte Ratio in Patients With Meningiomas

2020 ◽  
Vol 10 ◽  
Author(s):  
Yuki Kuranari ◽  
Ryota Tamura ◽  
Noboru Tsuda ◽  
Kenzo Kosugi ◽  
Yukina Morimoto ◽  
...  
Keyword(s):  
Prognostic Significance ◽  
Laboratory Data ◽  
Progression Free Survival ◽  
Cost Effective ◽  
Tumor Location ◽  
Neutrophil To Lymphocyte Ratio ◽  
Ki 67 ◽  
Who Grade ◽  
Lymphocyte Ratio ◽  
Subtotal Removal

BackgroundMeningiomas are the most common benign intracranial tumors. However, even WHO grade I meningiomas occasionally show local tumor recurrence. Prognostic factors for meningiomas have not been fully established. Neutrophil-to-lymphocyte ratio (NLR) has been reported as a prognostic factor for several solid tumors. The prognostic value of NLR in meningiomas has been analyzed in few studies.Materials and MethodsThis retrospective study included 160 patients who underwent surgery for meningiomas between October 2010 and September 2017. We analyzed the associations between patients’ clinical data (sex, age, primary/recurrent, WHO grade, extent of removal, tumor location, peritumoral brain edema, and preoperative laboratory data) and clinical outcomes, including recurrence and progression-free survival (PFS).ResultsForty-four meningiomas recurred within the follow-up period of 3.8 years. WHO grade II, III, subtotal removal, history of recurrence, Ki-67 labeling index ≥3.0, and preoperative NLR value ≥2.6 were significantly associated with shorter PFS (P < 0.001, < 0.001, 0.002, < 0.001, and 0.015, respectively). Furthermore, NLR ≥ 2.6 was also significantly associated with shorter PFS in a subgroup analysis of WHO grade I meningiomas (P = 0.003). In univariate and multivariate analyses, NLR ≥2.6 remained as a significant predictive factor for shorter PFS in patients with meningioma (P = 0.014).ConclusionsNLR may be a cost-effective and novel preoperatively usable biomarker in patients with meningiomas.

scholarly journals Clinicohistopathological study of astrocytomas along with Ki-67 proliferative index

2018 ◽  
Vol 6 (2) ◽  
pp. 665 ◽  
Author(s):  
Basumitra Das ◽  
Kurimella Vamsya Raj ◽  
Bhagyalakshmi Atla
Keyword(s):  
Histological Grade ◽  
Research Work ◽  
Low Grade ◽  
Proliferative Index ◽  
Ki 67 ◽  
Biologic Marker ◽  
Grade Ii ◽  
The Mean ◽  
The Difference ◽  
Grade Iii

Background: Astrocytomas form the largest group of gliomas (>75%) and diffusely infiltrating    accounting for more than 60% of all the primary brain tumors. The ki67 proliferative index is a potent biologic marker that estimates the growth of neoplasms quantitatively and thus will aid in identifying the prognosis for patients with neoplasms.  The aim of the research work was to study various histopathological and clinical features of Astrocytomas in detail, to evaluate Ki-67 proliferative index in patients of Astrocytomas and to compare the results of Immunohistochemistry with histological grade of Astrocytomas.Methods: A   total   number   of    40 cases of   Astrocytomas were included in the study.  Ki-67 immunostaining was done on all cases and compared with WHO histological grading of astrocytomas.Results: The mean Ki‑67 LI in Grade I astrocytomas was 4.66, range 4-5 ,  in Grade II astrocytomas mean was 8.07, range 5-12 ,in Grade III astrocytomas mean was 13.5 , range 8-20,  in Grade IV astrocytomas mean was 22.93, range 15-50. There was a highly significant correlation between the histopathological grade of astrocytomas and Ki-67 LI (p<0.05).Conclusions: The monoclonal antibody Ki-67 has proven its prognostic and diagnostic power in astrocytic tumors. Ki-67 LI is the simplest and the most reliable method for evaluating cell proliferation. Ki-67 LI increased with histological grade and the difference between low grade (I and II astrocytomas) and high grade (grade III and IV) is significant. In the present study Ki-67 LI is not dependent on factors like age and sex and is solely dependent on histological grade.

scholarly journals A comprehensive model including preoperative peripheral blood inflammatory markers for prediction of the prognosis of diffuse spinal cord astrocytoma following surgery

2021 ◽  
Author(s):  
Bo Pang ◽  
Rui-Chao Chai ◽  
Yao-Wu Zhang ◽  
Yu-Zhou Chang ◽  
Wei-Hao Liu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Peripheral Blood ◽  
Inflammatory Markers ◽  
Prognostic Model ◽  
Cox Regression ◽  
Histological Grade ◽  
Ki 67 ◽  
Who Grade ◽  
Grade Ii ◽  
Spinal Cord Astrocytoma

Abstract Purpose Due to the rarity of diffuse spinal cord astrocytoma, an effective model is still lacking to stratify their prognosis. Here, we aimed to establish a prognostic model through comprehensively evaluating clinicopathological features and preoperative peripheral blood inflammatory markers in 89 cases. Methods We performed univariate and multivariate Cox regression to identify prognosis factors. The Kaplan–Meier curves and ROC curves were employed to compare the prognostic value of selected factors. Results In addition to clinicopathological factors, we revealed the preoperative peripheral blood leukocyte count, neutrophils-to-lymphocytes ratio (NLR), and platelet-to-lymphocyte ratio (PLR) were also significantly correlated with overall survival of spinal cord astrocytoma in univariate Cox regression, and NLR was still significant in multivariate Cox analysis. Further, we demonstrated that NLR ≤ 3.65 and preoperative McCormick score (MMS) ≤ 3 were independently correlated with better survival of WHO grade IV tumors. Meanwhile, Ki-67 < 10% and resection extent ≥ 90% were independent prognostic factors in WHO grade II/III tumors. Finally, we developed a prognostic model that had better predictive efficiencies than WHO grade and histological grade for 1-year (AUC = 76.6), 2- year (AUC = 80.9), and 3-year (AUC = 80.3) survival. This model could classify tumors into 4 classifications with increasingly poor prognosis: 1, WHO grade II/III, with Ki-67 < 10% and resection extent ≥ 90%; 2, WHO grade II/III, Ki-67 ≥ 10% or resection < 90%; 3, WHO grade IV, NLR ≤ 3.65 and MMS ≤ 3; 4, WHO grade IV, with NRL > 3.65 or MMS = 4. Conclusion We successfully constructed a comprehensive prognostic model including preoperative peripheral blood inflammatory markers, which can stratify diffuse spinal cord astrocytoma into 4 subgroups.

scholarly journals Multiparametric and multiregional diffusion features help predict molecule information, grade and survival in lower-grade gliomas: a feasibility study

2019 ◽  
Vol 92 (1103) ◽  
pp. 20190324
Author(s):  
Hai Lin ◽  
Yanwen Xu ◽  
Lei Chen ◽  
Peng Na ◽  
Weiping Li
Keyword(s):  
Support Vector Machine ◽  
Cox Regression ◽  
Diffusion Tensor ◽  
Histological Grade ◽  
Area Under The Curve ◽  
Support Vector ◽  
Lower Grade ◽  
Ki 67 ◽  
Who Grade ◽  
Grade Ii

Objective: This study was to investigate the relationship of diffusion features with molecule information, and then predict grade and survival in lower-grade gliomas. Methods: 65 patients with primary lower-grade gliomas (WHO Grade II & III) who underwent conventional MRI and diffusion tensor imaging were retrospectively studied. The tumor region was automatically segmented into contrast-enhancing tumor, non-enhancing tumor, edematous and necrotic volumes. Diffusion features, including fractional anisotropy (FA), axial diffusivity, radial diffusivity and apparent diffusion coefficient (ADC), were extracted from each volume using histogram analysis. To estimate molecule biomarkers and predict clinical characteristics of grade and survival, support vector machine, generalized linear model, logistic regression and Cox regression were performed on the related features. Results: The diffusion features in non-enhancing tumor volume showed differences between isocitrate dehydrogenase mutant and wild-type gliomas. And the mean accuracy of support vector machine classifiers was 0.79. Ki-67 labeling index was correlated with these features, which were combined to significantly estimate Ki-67 expression level (r = 0.657, p < 0.001). These features also showed differences between Grade II and III gliomas. A combination of them for grade classification resulted in an area under the curve of 0.914 (0.857–0.971). Mean FA and fifth percentile of ADC were independently associated with overall survival, with lower FA and higher ADC showing better survival outcome. Conclusion: In lower-grade gliomas, multiparametric and multiregional diffusion features could help predict molecule information, histological grade and survival. Advances in knowledge: The multi parametric diffusion features in non-enhancing tumor were associated with molecule information, grade and survival in lower-grade gliomas.

scholarly journals Diagnostic value of microvessel structure in brain glial tumors

2017 ◽  
Vol 25 (3) ◽  
pp. 350-361 ◽  
Author(s):  
I. S. Shpon’ka ◽  
T. V. Shynkarenko
Keyword(s):  
Prognostic Significance ◽  
Diagnostic Value ◽  
Proliferation Index ◽  
Ki 67 ◽  
Who Grade ◽  
Vascular Area ◽  
Diffuse Gliomas ◽  
The Mean ◽  
Brain Gliomas ◽  
Diffuse Brain

Diffuse gliomas are the most common primary brain tumors with a disproportionately high mortality rate. Characteristics of microvessels are of high diagnostic and prognostic significance, however, the results of previous studies are controversial. The aim of the work is to evaluate the features of angiogenesis in diffuse gliomas on the basis of determining the qualitative and quantitative microvascular characteristics. Also important is their relationship with the histological type of tumor. Microvascular density (μm-1), total vascular area (%), total lumen area (%) and the mean diameter of microvessels (μm) were measured and calculated in diffuse brain gliomas (n=76) using GFAP-negative status of endothelium in the presence of exclusively GFAP-positive tumor cells. Proliferation of microvessels was evaluated using proliferation index of vascular epithelium (Ki-67). The possibility of routine evaluation of the angiogenesis in diffuse gliomas using GFAP and Ki-67 markers was defined. We revealed significant correlation between features of the neoplastic microvasculature and WHO Grade.

From glioblastoma to gangliocytoma: an unforeseen but welcome shift in biological behavior

2009 ◽  
Vol 4 (5) ◽  
pp. 475-478 ◽  
Author(s):  
Astrid Jeibmann ◽  
Martin Hasselblatt ◽  
Stefan Pfister ◽  
Ronald Sträter ◽  
Angela Brentrup ◽  
...  
Keyword(s):  
Prognostic Significance ◽  
Genetic Alterations ◽  
Biological Behavior ◽  
Proliferation Index ◽  
Comparative Genomic ◽  
Biological Mechanisms ◽  
Ki 67 ◽  
Who Grade ◽  
Chemotherapy Sensitivity ◽  
Neuronal Markers

The prognosis in children harboring a glioblastoma multiforme (GBM) is usually poor. Few GBMs in children, however, seem to respond quite well to adjuvant chemotherapy. The biological basis for such chemotherapy sensitivity remains uncertain. In this paper the authors report the case of a 2-month-old girl with a histologically confirmed GBM (WHO Grade IV) in whom chemotherapy was accompanied by differentiation of the malignant primary tumor into a typical gangliocytoma (WHO Grade I) showing ganglioid differentiation and expression of neuronal markers synaptophysin, neurofilament, and NeuN as well as a low Ki 67/MIB-1 proliferation index. Array-comparative genomic hybridization did not reveal genetic alterations in either specimen. Even though the underlying biological mechanisms remain to be elucidated, closer examination of frequency and prognostic significance of neuronal differentiation in pediatric GBMs within ongoing and future clinical trials may be warranted.

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