Predictive value of p53 and ki67 in different grades of meningioma

2018 ◽  
Vol 9 (SPL1) ◽  
Author(s):  
Sarah ali Abed ◽  
Sabah Ahmed Abid Abu Sabe
Keyword(s):  
Histological Grade ◽  
Suppressor Gene ◽  
Malignant Meningioma ◽  
Ki 67 ◽  
Who Grade ◽  
Brain Invasion ◽  
The Central Nervous System ◽  
The Mean ◽  
Proliferative Marker ◽  
The Brain

Meningiomas are common tumors of the central nervous system that originate from the meningeal coverings of the brain and the spinal cord. Many factors are involved in tumor progression,one of the important factors is mutation of tumor suppressor gene p53. Ki-67 is considered to be the most reliable proliferative marker predicting tumor behavior.The aim of the present study is to evaluate the use ofimmunhistochemical expression of p53 and ki67 for predicting the grades of meningioma which are important in their prognosis.Sixty patients with different grades of meningioma were taken. Histological sections of the paraffin embedded tissues of these cases were be taken for H&E staining for assessment of histological grade according to WHO grading system. Immunohistochemical staining of sections of the paraffin embedded tissues by using monoclonal antibody for P53 & ki-67 were done. Correlation between tumor grades and immunhistochemical markers were done.The presences of mitosis,sheeting,prominent nucleoli,hypercellularity,pleomorphism,and the necrosis,except brain invasion,were correlated with tumor grades. The mean Ki-67 labeling index was significantly higher in meningiomas of WHO grade III than in those of grades I and II. Overexpression of p53 was found mainly in the atypical and malignant meningioma group. There is a good and positive correlation between Ki-67 and p53 expression.The Ki-67 proliferative marker and p53 are both presented highly in atypical and malignant meningioma,the routine use of them in predicting the behavior of meningiomas may be advocated based on the results of this study.

scholarly journals 1. Traditional and Electronic Ki-67 Quantitation in Oligodendrogliomas

2015 ◽  
Vol 42 (S2) ◽  
pp. S2-S2
Author(s):  
S. Asiry ◽  
P. Rizek ◽  
R. Hammond
Keyword(s):  
Histological Grade ◽  
Prognostic Significance ◽  
Proliferative Index ◽  
Ki 67 ◽  
Proliferation Markers ◽  
Who Grade ◽  
The Central Nervous System ◽  
High Concordance ◽  
Gastroenteropancreatic Neuroendocrine Tumours ◽  
Automated Methods

The Ki-67 proliferative index has become a useful, objective, immunohistochemical tool that can aid in grading and prognostication for patients with oligodendrogliomas. Previous studies have described the prognostic significance of the Ki-67 index for such patients.According to the WHO classification of tumors of the central nervous system (2007) ”mitotic activity is low in WHO grade II oligodendroglioma, and labeling indices for proliferation markers are accordingly low, usually below 5%”. Furthermore, the predictive value of the Ki-67 index appears to be independent of age, tumor site, and histological grade. What is less well described is the relative accuracy of traditional vs. semi-automated methods of enumeration for a test where small differences can influence grading, prognosis and treatment. Tang et al. (2012), studying gastroenteropancreatic neuroendocrine tumours, found high concordance between two semi-automated methods for Ki-67 quantitation whereas “eyeballed estimates” were far less reliable. We will compare the reported proliferative index estimates to those calculated by digital image analysis of 35 recent oligodendrogliomas from the LHSC Pathology archives.

scholarly journals Clinicohistopathological study of astrocytomas along with Ki-67 proliferative index

2018 ◽  
Vol 6 (2) ◽  
pp. 665 ◽  
Author(s):  
Basumitra Das ◽  
Kurimella Vamsya Raj ◽  
Bhagyalakshmi Atla
Keyword(s):  
Histological Grade ◽  
Research Work ◽  
Low Grade ◽  
Proliferative Index ◽  
Ki 67 ◽  
Biologic Marker ◽  
Grade Ii ◽  
The Mean ◽  
The Difference ◽  
Grade Iii

Background: Astrocytomas form the largest group of gliomas (>75%) and diffusely infiltrating    accounting for more than 60% of all the primary brain tumors. The ki67 proliferative index is a potent biologic marker that estimates the growth of neoplasms quantitatively and thus will aid in identifying the prognosis for patients with neoplasms.  The aim of the research work was to study various histopathological and clinical features of Astrocytomas in detail, to evaluate Ki-67 proliferative index in patients of Astrocytomas and to compare the results of Immunohistochemistry with histological grade of Astrocytomas.Methods: A   total   number   of    40 cases of   Astrocytomas were included in the study.  Ki-67 immunostaining was done on all cases and compared with WHO histological grading of astrocytomas.Results: The mean Ki‑67 LI in Grade I astrocytomas was 4.66, range 4-5 ,  in Grade II astrocytomas mean was 8.07, range 5-12 ,in Grade III astrocytomas mean was 13.5 , range 8-20,  in Grade IV astrocytomas mean was 22.93, range 15-50. There was a highly significant correlation between the histopathological grade of astrocytomas and Ki-67 LI (p<0.05).Conclusions: The monoclonal antibody Ki-67 has proven its prognostic and diagnostic power in astrocytic tumors. Ki-67 LI is the simplest and the most reliable method for evaluating cell proliferation. Ki-67 LI increased with histological grade and the difference between low grade (I and II astrocytomas) and high grade (grade III and IV) is significant. In the present study Ki-67 LI is not dependent on factors like age and sex and is solely dependent on histological grade.

Squash Cytology of a Dural-Based High-Grade Chondrosarcoma May Mimic That of Glioblastoma in the Central Nervous System

2015 ◽  
Vol 59 (2) ◽  
pp. 219-224 ◽  
Author(s):  
Sangho Lee ◽  
Na Rae Kim ◽  
Dong Hae Chung ◽  
Gi-Taek Yee ◽  
Hyun Yee Cho
Keyword(s):  
Magnetic Resonance Images ◽  
Temporal Area ◽  
Brain Invasion ◽  
S 100 ◽  
Radiation History ◽  
The Central Nervous System ◽  
Meningeal Tumors ◽  
Memory Disturbance ◽  
Well Differentiated ◽  
The Brain

Background: Intracranial chondrosarcoma is rare, and most cases occur in the skull base. Intradural chondrosarcoma is even rarer. Case: Here, we describe a case of dural chondrosarcoma with a radiation history for nasopharyngeal carcinoma and a radical prostatectomy for prostatic cancer 15 and 8 years earlier, respectively. A 67-year-old man presented with a 3-week memory disturbance and dysarthria. Computed tomography and magnetic resonance images of the brain revealed a dural-based mass in the left temporal area. Under the impression of a glioblastoma, a resection and an intraoperative squash cytology were done. A necrotic dirty background as well as bluish-to-pinkish myxoid stroma were characteristic; the nuclei of highly pleomorphic tumor cells were hyperchromatic to vesicular with an occasional ground-glass appearance. The cytoplasm was of an eosinophilic hyalinized condensed morphology with an occasional granular appearance. Histologically, the lobulated mass was composed of hypercellular lobules of well-differentiated chondrocytes intermixed with anaplastic pleomorphic cells and diagnosed as a conventional grade III chondrosarcoma. These cells were immunoreactive for D2-40, S-100 protein and vimentin. Brain invasion was also found. Conclusion: Albeit rare, dural-based chondrosarcomas should be considered in the differential diagnosis for meningeal tumors, especially in the case of previous radiation therapy.

scholarly journals Gross Morphology of the Brain and Spinal Cord of the African Pygmy Hedgehog (Atelerix albiventris)

2021 ◽  
Vol 65 (3) ◽  
pp. 15-21
Author(s):  
O. O. Igado ◽  
S. F. Braimah ◽  
A. A. Obasa
Keyword(s):  
Spinal Cord ◽  
Foramen Magnum ◽  
Linear Measurements ◽  
Olfactory Bulbs ◽  
The Central Nervous System ◽  
The Mean ◽  
Brain And Spinal Cord ◽  
The Brain ◽  
Average Body ◽  
Gross Examination

Abstract The African pygmy hedgehog (Atelerix albiventris) is an insectivorous animal, native to Africa. The central nervous system (CNS) consists of the brain and the spinal cord, protected by the cranium and vertebral column respectively. Assessment of the gross appearance and morphometries of the African pygmy hedgehog CNS were carried out using six adults (3 males and 3 females). The gross examination showed the brains to be lissencephalic, with relatively large olfactory bulbs, similar to that observed in some rodents. The rootlets of the first cervical spinal nerves were observed to emerge before the foramen magnum. Linear measurements were obtained from both the brain and spinal cord. The mean weight of the animals was 199.00 ± 16.09 g, with the males having an average body weight of 183.50 ± 12.02 g and the females 206.80 ± 11.95 g. Although not statistically significant, the males had a higher encephalisation quotient (0.40 ± 0.08) relative to the females 0.36 ± 0.04). The values for the brain weight, length of spinal cord and heights of the telencephalon and diencephalon at different points were higher in the males, while the spinal cord weight, length of brain and cerebellar height were higher in the females. The spinal cord showed slight enlargements at the cervical, thoracic, lumbar and sacral segments. This study aimed to provide baseline data for the study of the gross appearance and neuromorpho-metrics of the hedgehog, with possible application in regional anaesthesiology and comparative wildlife neuroanatomy.

Penciclovir Pharmacokinetics and Distribution to the Brain and Muscle of Rats, Studied by Microdialysis

1997 ◽  
Vol 8 (3) ◽  
pp. 275-279 ◽  
Author(s):  
N Borg ◽  
L Ståhle
Keyword(s):  
Concentration Ratio ◽  
Antiviral Agents ◽  
Post Injection ◽  
Herpes Encephalitis ◽  
Oral Form ◽  
Muscle Concentration ◽  
The Central Nervous System ◽  
The Mean ◽  
The Brain ◽  
Versus Concentration

Famciclovir, the oral form of penciclovir, is a potent, highly selective antiherpesvirus agent licenced for the treatment of herpes zoster (shingles). Some herpesviruses are prone to infect the central nervous system. To obtain guidance for the possible treatment of herpes encephalitis it is important to study the extent of transport of antiviral agents into the brain. We have used microdialysis to sample the unbound extracellular concentration of penciclovir in the gastrocnemic muscle (which corresponds directly to plasma free concentrations) and in the brain of rats under halothane anaesthesia. Penciclovir (50 mg kg−1) was given intravenously (i.v.) and samples were taken for 5 h after administration. The AUC (area under the time versus concentration curve) (0–5 h) of penciclovir in the brain was 0.096±0.018 (mean±SEM) of the AUC in muscle while the mean ratio of brain to muscle concentration 5 h post-injection was 0.1 80±0.084. Famciclovir given per os to rat at a dose of 1 20 mg kg−1 resulted in a concentration ratio for penciclovir between brain and muscle of 0.415±0.078 at 5 h after administration, while the AUC ratio (0–5 h) was 0.143±0.012. Both of these are higher than after i.v. injection of penciclovir. Penciclovir and famciclovir were also administrated by i.v. infusion (60 and 80 mg kg−1 h−1 respectively). Famciclovir administration (AUC 0.075±0.025 mmol h L−1) did not increase penciclovir transport to the brain compared with penciclovir administration (AUC 0.163±0.018 mmol h L−1).

scholarly journals A comprehensive model including preoperative peripheral blood inflammatory markers for prediction of the prognosis of diffuse spinal cord astrocytoma following surgery

2021 ◽  
Author(s):  
Bo Pang ◽  
Rui-Chao Chai ◽  
Yao-Wu Zhang ◽  
Yu-Zhou Chang ◽  
Wei-Hao Liu ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Peripheral Blood ◽  
Inflammatory Markers ◽  
Prognostic Model ◽  
Cox Regression ◽  
Histological Grade ◽  
Ki 67 ◽  
Who Grade ◽  
Grade Ii ◽  
Spinal Cord Astrocytoma

Abstract Purpose Due to the rarity of diffuse spinal cord astrocytoma, an effective model is still lacking to stratify their prognosis. Here, we aimed to establish a prognostic model through comprehensively evaluating clinicopathological features and preoperative peripheral blood inflammatory markers in 89 cases. Methods We performed univariate and multivariate Cox regression to identify prognosis factors. The Kaplan–Meier curves and ROC curves were employed to compare the prognostic value of selected factors. Results In addition to clinicopathological factors, we revealed the preoperative peripheral blood leukocyte count, neutrophils-to-lymphocytes ratio (NLR), and platelet-to-lymphocyte ratio (PLR) were also significantly correlated with overall survival of spinal cord astrocytoma in univariate Cox regression, and NLR was still significant in multivariate Cox analysis. Further, we demonstrated that NLR ≤ 3.65 and preoperative McCormick score (MMS) ≤ 3 were independently correlated with better survival of WHO grade IV tumors. Meanwhile, Ki-67 < 10% and resection extent ≥ 90% were independent prognostic factors in WHO grade II/III tumors. Finally, we developed a prognostic model that had better predictive efficiencies than WHO grade and histological grade for 1-year (AUC = 76.6), 2- year (AUC = 80.9), and 3-year (AUC = 80.3) survival. This model could classify tumors into 4 classifications with increasingly poor prognosis: 1, WHO grade II/III, with Ki-67 < 10% and resection extent ≥ 90%; 2, WHO grade II/III, Ki-67 ≥ 10% or resection < 90%; 3, WHO grade IV, NLR ≤ 3.65 and MMS ≤ 3; 4, WHO grade IV, with NRL > 3.65 or MMS = 4. Conclusion We successfully constructed a comprehensive prognostic model including preoperative peripheral blood inflammatory markers, which can stratify diffuse spinal cord astrocytoma into 4 subgroups.

scholarly journals Comparison of the Position Sense of the Knee Joint in PatientsWith Multiple Sclerosis and Healthy Controls

2019 ◽  
Author(s):  
Saina Aliabadi ◽  
Roya Khanmohammadi ◽  
Gholamrezareza Olyaei ◽  
Nastaran Ghotbi ◽  
Saeed Talebian ◽  
...  
Keyword(s):  
Multiple Sclerosis ◽  
Nervous System ◽  
Knee Joint ◽  
Healthy Subjects ◽  
Position Sense ◽  
The Body ◽  
Control Group ◽  
The Central Nervous System ◽  
The Mean ◽  
The Brain

Introduction: Position sense, one of the most accurate senses in the body, makes everyone aware of the state of the body in space. This sense is an essential ability in maintaining physical health and avoiding injury. Deficits in position sense cause balance impairments in people with mild Multiple Sclerosis (MS). Position sense requires instant and coordinated communication between the central nervous system and peripheral nervous system, while in patients with MS, communication between the brain and other parts of the body is disrupted. This study aims to compare the position sense of knee joint in people with MS and healthy subjects. Materials and Methods: Ten healthy subjects with the Mean±SD age of 27.6±3.71 years and 10 persons with MS disease and the Mean±SD age of 31.40±3.50 years participated in this study. For evaluating their position sense of knee joint, they flexed their knees (from 90 to 45 degrees) four times, and then a software calculated their repositioning errors. Results: No significant changes in repositioning errors (constant, variable, absolute) were observed in MS patients, and the control group (P˃0.05). Conclusion: The results indicate that mild MS disease cannot disturb the position sense of knee joint.

scholarly journals Brain weights in adult africans

2017 ◽  
Vol 34 (04) ◽  
pp. 223-225 ◽  
Author(s):  
A. Salami ◽  
M. Ajani ◽  
I. Orhorho ◽  
G. Ogun ◽  
A. Adeoye ◽  
...  
Keyword(s):  
Early Adulthood ◽  
Age Groups ◽  
The Elderly ◽  
Brain Weight ◽  
Significant Difference ◽  
The Central Nervous System ◽  
The Mean ◽  
The Difference ◽  
Autopsy Specimens ◽  
The Brain

Abstract Introduction: The average brain weight of adult humans, using Caucasian figures, is said to be between 1300g to 1400g. Few studies have however been done to make actual evaluations of brain weights in adult Africans. This study seeks to examine the weight of brains from people of African descent with respect to variations in sex and age in decades using autopsy specimens. Materials and Methods: Analysis of the weight of brains removed from both male and female adult patients during fresh autopsy of their bodies in our center over a ten year period was done. The study criteria required non-involvement of the central nervous system in the cause of death. The brains were grouped based on age in decades and further grouped into early, middle and late age groups. Descriptive statistical analysis was done using SPSS 20 statistics software. Results: A total of one hundred and sixteen brains were included in the study and the mean brain weight was 1280g with a range between 1015g to 1590g. There was no statistically significant difference in the mean brain weight of the different age groups. The average male brain was heavier than those of females and the difference was statistically signiicant. Conclusion: The brain weight of adult Africans in our study is similar to that seen in Caucasians. There is no statistically significant difference in the brain weight of adults from early adulthood to the elderly adults. Male adults have statistically heavier brains than the females.

scholarly journals Multiparametric and multiregional diffusion features help predict molecule information, grade and survival in lower-grade gliomas: a feasibility study

2019 ◽  
Vol 92 (1103) ◽  
pp. 20190324
Author(s):  
Hai Lin ◽  
Yanwen Xu ◽  
Lei Chen ◽  
Peng Na ◽  
Weiping Li
Keyword(s):  
Support Vector Machine ◽  
Cox Regression ◽  
Diffusion Tensor ◽  
Histological Grade ◽  
Area Under The Curve ◽  
Support Vector ◽  
Lower Grade ◽  
Ki 67 ◽  
Who Grade ◽  
Grade Ii

Objective: This study was to investigate the relationship of diffusion features with molecule information, and then predict grade and survival in lower-grade gliomas. Methods: 65 patients with primary lower-grade gliomas (WHO Grade II & III) who underwent conventional MRI and diffusion tensor imaging were retrospectively studied. The tumor region was automatically segmented into contrast-enhancing tumor, non-enhancing tumor, edematous and necrotic volumes. Diffusion features, including fractional anisotropy (FA), axial diffusivity, radial diffusivity and apparent diffusion coefficient (ADC), were extracted from each volume using histogram analysis. To estimate molecule biomarkers and predict clinical characteristics of grade and survival, support vector machine, generalized linear model, logistic regression and Cox regression were performed on the related features. Results: The diffusion features in non-enhancing tumor volume showed differences between isocitrate dehydrogenase mutant and wild-type gliomas. And the mean accuracy of support vector machine classifiers was 0.79. Ki-67 labeling index was correlated with these features, which were combined to significantly estimate Ki-67 expression level (r = 0.657, p < 0.001). These features also showed differences between Grade II and III gliomas. A combination of them for grade classification resulted in an area under the curve of 0.914 (0.857–0.971). Mean FA and fifth percentile of ADC were independently associated with overall survival, with lower FA and higher ADC showing better survival outcome. Conclusion: In lower-grade gliomas, multiparametric and multiregional diffusion features could help predict molecule information, histological grade and survival. Advances in knowledge: The multi parametric diffusion features in non-enhancing tumor were associated with molecule information, grade and survival in lower-grade gliomas.

scholarly journals Primary lymphoma of the central nervous system: a clinical-pathological and immunohistochemical study of ten autopsy cases

2006 ◽  
Vol 64 (4) ◽  
pp. 976-982 ◽  
Author(s):  
Henrique Costa ◽  
Marcello Franco ◽  
Myriam Dumas Hahn
Keyword(s):  
Central Nervous System ◽  
Nervous System ◽  
B Cell ◽  
Laboratory Data ◽  
Primary Lymphoma ◽  
System A ◽  
Long Time ◽  
The Central Nervous System ◽  
The Mean ◽  
The Brain

CONTEXT: Primary central nervous system lymphomas (PCNSL) are a rare subgroup of lymphomas generally associated with HIV and EBV. OBJECTIVE: To study ten autopsy cases of PCNSL, to describe the neuropathological findings, to characterize the phenotype of the neoplastic cells, to detect EBV in the lesion and to compare the findings with the clinical and laboratory data of the patients. METHOD: The clinical, histological and immunohistochemical data of ten cases of PCNSL, eight cases from patients with AIDS, identified among 265 autopsies of these patients were analyzed. RESULTS: Seven patients were males and the mean age was 40.9 years. The most frequent symptomatology was focal neurologic deficit (70%). Six patients presented with only one lesion. Histologically, densely cellular and polymorphous neoplasms with angiocentrism were observed, in 90% of cases. An association with other diseases was observed in four cases. Most patients had diffuse large B cell non-Hodgkin’s lymphoma. EBV was detected by immunohistochemistry in only one case. The lack of detection of the virus might have been due to the long time of fixation of the brain which might have inactivate epitopes therefore compromising the testing. CONCLUSION: In the present series, PCNSL presented with focal symptoms, with unifocal or multifocal lesions, with a predominant B-cell CD20 positive phenotype, rarely associated with EBV.

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