Effect of supplementary crude protein level and degradability in grass silage-based diets on performance of dairy cows, and digestibility and abomasal nitrogen flow in sheep

1990 ◽  
Vol 51 (2) ◽  
pp. 235-244 ◽  
Author(s):  
R. F. Cody ◽  
J. J. Murphy ◽  
D. J. Morgan

ABSTRACTFour concentrate supplements differing in crude protein (CP) and undegradable protein (UDP) content were offered to 16 lactating Friesian cows together with grass silage (dry matter (DM) 196 g/kg, pH 4·38, CP 160 g/kg DM, in vitro DM digestibility 0·68)ad libitumin a Latin-square trial with 3-week periods. The supplement treatments were: (1) barley 122 g CP per kg DM, degradability (dg) 0·77; (2) barley/soya-bean meal 210 g CP per kg DM, dg 0·69; (3) barley/soya-bean meal/fish meal 190 g CP per kg DM, dg 0·61; (4) barley/soya-bean meal/fish meal 219 g CP per kg DM, dg 0·59. Supplements were given at 8 kg/day. Total daily intakes of silage (kg DM), CP and UDP (g) on treatments 1 to 4 were 7·77, 2087, 375; 8·35, 2804, 655; 8·29, 2676, 717; 8·70, 2917, 826, respectively. Milk yield (kg/day) and yields of fat, protein and lactose (g/day) on the four treatments were 21·3, 791, 617, 984; 23·0, 816, 688, 1055; 23·0, 818, 696, 1050; 23·6, 813, 735, 1071 for treatments 1 to 4 respectively. Yield and concentration of protein and lactose were significantly lower on treatment 1 than on the other treatments, while the of blood metabolites indicated treatment effects on blood glucose, non-esterified fatty acids, plasma protein and urea. Digestibility of organic matter and non-ammonia nitrogen (NAN) flow to the abomasum (g/day), measured in sheep given a fixed silage/supplement ratio at maintenance, were 0·81 and 18·4, 0·81 and 20·8, 0·82 and 21·4, 0·82 and 22·4 for treatments 1 to 4 respectively. The NAN flow was significantly greater on treatment 4 than on treatment 1.

1992 ◽  
Vol 54 (3) ◽  
pp. 333-339 ◽  
Author(s):  
R. W. J. Steen

AbstractTwo experiments were carried out to evaluate extracted soya-bean meal, fish meal and maize gluten feed as protein supplements for calves offered grass silage ad libitum. The five treatments used in experiment 1 consisted of 1·4 kg per head daily of concentrate dry matter (DM) consisting of (g/kg) 50 molasses and 25 minerals and vitamins plus (1) 925 barley (B), (2) 700 B and 225 extracted soya-bean meal (SBM), (3) 785 B and 140 fish meal (FM), (4) 925 maize gluten feed (MGF) and (5) 2·25 kg DM per head daily of concentrate (1). Those used in experiment 2 consisted of 1·4 kg per head daily of concentrate DM consisting of (g/kg) (1) 1000 B, (2) 800 B and 200 SBM, (3) 860 B and 140 FM, (4) 1000 MGF and (5) 2·25 kg DM per head daily of B. In each experiment the diets were offered to 70 calves which were initially 131 and 130 kg live weight, for periods of 14 and 13 weeks in experiments 1 and 2 respectively. The silages contained 155 and 154 g crude protein (CP) per kg DM; 45 and 77 g ammonia-nitrogen per kg total nitrogen and 698 and 726 g digestible organic matter per kg DM in experiments 1 and 2. Concentrates 1 and 5 contained 104 to 110 g CP per kg DM and concentrates 2, 3 and 4 contained 195 to 204 g CP per kg DM in both experiments. For diets 1 to 5 respectively silage DM intakes were 2·31,2·50,2·53,2·59 and 1 -61 (s.e. 0·097) kg/day; live-weight gains were 0·84,0·98,1 -01, 0·88 and 0·95 (s.e. 0·032) kg/day in experiment 1 and 2·38, 2·53, 2·54, 2·82 and 1·77 (s.e. 0·045) kg/day and 0·90,1·04,1·03,1·05 and 0·99 (s.e. 0·038) kg/day for experiment 2. It is concluded that the use of soya-bean meal rather than fish meal did not affect silage intake or animal performance. Replacing mixtures of barley and soya-bean meal or barley and fish meal with an equal quantity of isonitrogenous maize gluten feed did not affect animal performance in one experiment but significantly reduced it in the other experiment.


1989 ◽  
Vol 48 (1) ◽  
pp. 81-89 ◽  
Author(s):  
R. W. J. Steen

ABSTRACTTwo experiments were carried out to examine the relative value of soya-bean, sunflower and fish meals as protein supplements for growing beef cattle offered grass silage ad libitum. In each experiment, well preserved grass silage (116 and 119 g crude protein (CP) per kg dry matter (DM) and 69 and 76 g ammonia-N per kg total N in experiments 1 and 2 respectively) was supplemented with (1) barley (107 g CP per kg DM), (2) barley/soya-bean meal (224 g CP per kg DM), (3) barley/sunflower meal (223 g CP per kg DM) and (4) barley/fish meal (218 g CP per kg DM). Each of the supplements was offered at a rate of 1·3 kg per head daily to 32 British Friesian castrated male cattle (mean initial live weight 334 kg) n i each experiment. The treatments were imposed for 118 and 134 days in experiments 1 and 2 respectively and the animals were slaughtered 8 months after the end of the treatment periods. On average over the two experiments for supplements 1 to 4 respectively, silage DM intakes were 6·2, 6·3, 6·3 and 6·2 (s.e. 0·053) kg/day; live-weight gains 0·69, 0·78, 0·71 and 0·80 (s.e. 0·039) kg/day; carcass weights 375, 377, 377 and 374 (s.e. 8·1) kg, carcass fat classification (1 = leanest, 5 = fattest); 3·5, 3·5, 3·4 and 3·6 (s.e. 0·14) and areas of m. longissimus dorsi 19·8, 19·9, 19·7 and 19·3 (s.e. 0·66) mnv/kg carcass weight. It is concluded that when part of a barley supplement given in addition to well preserved grass silage was replaced with soya-bean, sunflower or fish meals, silage intake was not affected. The inclusion of fish meal in the diet increased live-weight gain by HOg/day and the response to soya-bean meal was 0·78 of the response to fish meal per unit of crude protein. Sunflower meal did not affect performance. The treatments did not affect carcass weight or fatness 8 months after the end of the treatment periods.


1979 ◽  
Vol 29 (2) ◽  
pp. 257-269 ◽  
Author(s):  
J. J. Robinson ◽  
I. McHattie ◽  
J. F. Calderon Cortes ◽  
J. L. Thompson

ABSTRACTFour experiments were made during early lactation on individually penned Finnish Landrace × Dorset Horn ewes, each suckling two lambs. In Experiment 1, 14 ewes of mean body weight 74 ± 2·4 kg received daily, 1·9 kg dry matter, 20 MJ of metabolizable energy and 300 g of crude protein from a diet (H) containing hay, barley and fish meal. Reduction of the daily crude protein intake of half the ewes to 225 g by replacing fish meal with barley (diet L) in week 2 of lactation resulted in a decrease (P< 0·01) in daily milk yield of 0·53 kg (17%) compared with no change for ewes remaining on diet H. The effect was reversed by returning the ewes to diet H at the end of week 3. In Experiment 2 the use of four ewes fitted with abomasal cannulae showed that the additional non-ammonia nitrogen reaching the abomasum when fish meal was given was used with an efficiency of 0·51 for the production of milk nitrogen. In Experiment 3, involving 20 ewes, a restriction in protein intake for more than 10 days in early lactation by replacing the fish meal with barley depressed subsequent milk yield. In Experiment 4, 24 ewes were given either fish meal, soya bean meal or groundnut meal as protein supplements. In early lactation fish meal was superior by 0·40 and 0·43 kg of milk per day to soya bean meal and groundnut meal respectively, and this was attributed to the greater quantity of non-ammonia nitrogen reaching the abomasum in the fish meal compared with the soya bean meal and groundnut meal supplements. The commercial significance of the results is discussed.


1985 ◽  
Vol 41 (3) ◽  
pp. 293-300 ◽  
Author(s):  
R. W. J. Steen

AbstractTwo experiments were carried out to examine the effects on the intake and performance of calves of supplementing a diet, based on high-digestibility, well-preserved grass silage, with fish meal. The effects of fish-meal supplementation were examined at each of two amounts of concentrate intake (1·05 and 2·2 kg/day) in a 2 × 2 factorial design. Fish meal was offered at a rate of 75 g/kg silage dry-matter (DM) intake. Experiments 1 and 2 involved 36 and 40 castrated male calves, respectively, which were initially 7 weeks old and had a live weight of 70 kg. The treatments were imposed for 20 weeks. The silages used in the two experiments had mean concentrations of 22-8 g nitrogen (N) per kg DM; 524 g protein N per kg total N; 59 g ammonia N per kg total N; 0·4 g butyric acid per kg DM; and 712 g digestible organic matter per kg DM. The concentrate consisted of (g/kg) 800 ground barley, 125 soya bean meal, 50 molasses and 25 mineral and vitamin supplement, respectively, and contained 29 g N per kg DM. Silage DM intakes and live-weight gains for the low and high amounts of concentrate supplementation were 1·93 and 1·46 (s.e. 0·055) kg/day and 0·84 and 1·01 (s.e. 0·013) kg/day respectively. The supplement of fish meal did not affect silage DM intake or animal performance for either concentrate intake. Proportionately, only 0·21 of the difference in live weight at the end of the treatment periods between the two amounts of concentrate intake remained at slaughter at 18 months of age. It is concluded that the optimum growth rate for autumn-born calves during their first winter can be sustained by a diet of high-digestibility grass silage supplemented with a maximum of 1·0 kg cereal-soya bean meal concentrate and that no response in performance was obtained to supplementation of this diet with fish meal.


Author(s):  
A T Chamberlain

The metabolisabie protein system (AFRC, 1992) requires the measurement of the dynamics of the degradation of protein in the rumen. However the recommended method, using fistulated animals, is slow, expensive and may be considered unacceptable by many in terms of animal welfare. The objective of this work was to develop an in vitro technique for the routine assessment of feed protein degradability.A 101 vessel fitted with an automatic stirrer was filled with modified van Soest media and rumen liquor (pooled from two sheep) and maintained under anaerobic conditions at 39.5°C. Small artificial fibre bags (4.5x3cm, pore size 54 micro-m ±4) were filled with 0.4g of soya bean meal and incubated in the vessel for 3,6,10 and 24 hours using 3 replicates at each time. Bags were washed in running cold water for 30 mins immediately after removal. Four replicates of 3g samples in large Dacron bags (10x7cm, pore size 55 micro-m ±22) were also incubated in sacco in fistulated yearling wethers using a 4x4 Latin square design to assess the base line degradability. In sacco bags were rinsed and frozen after removal and washed in a domestic washing machine at the end of the experiment.


1987 ◽  
Vol 57 (1) ◽  
pp. 109-114 ◽  
Author(s):  
Gerald B. Huntington

1. The objective of the present study was to evaluate effects of in vitro rumen solubility or degradability of dietary protein on net absorption of nitrogenous compounds from portal-drained viscera of beef heifers.2. Four protein sources, casein, soya-bean meal, maize-gluten meal and blood meal provided about two-thirds of total dietary nitrogen in semi-purified diets given to four beef heifers in a 4 x 4 Latin square design.3. Although in vitro degradability of dietary N ranged from 842 (casein) to 310 (blood meal) g/kg total dietary N, net absorption of ammonia-N or α-amino-N was not significantly different (P > 0.10) among diets. However, net absorption of NH3-N tended to decrease and net absorption of α-amino-N tended to increase as intake of in vitro undegradable N increased. Net transfer of urea-N from plasma to portal-drained viscera was greater (P < 0.10) when heifers were fed on soya-bean meal than when fed on maize-gluten meal or blood meal.4. The findings show with all diets that the non-protein-nitrogenous compounds, NH3 and urea, played a substantial role in absorption and transfer of N. Overall, net absorption of NH3-N was 61% of net absorption of α-amino-N, and transfer of urea-N from plasma to portal-drained viscera was 80% ofnet absorption of α-amino-N.


2018 ◽  
Vol 26 (1) ◽  
pp. 87-99
Author(s):  
J.R. Solomon ◽  
I.A. Abawale

The growth performance of catfish Clarias gariepinus using imported feeds and locally made feeds of the same crude protein were compared. C. gariepinus (mean weight 12.8g) juvenile were reared in cages for a period of 12 weeks on different feed. Two imported feeds (skretting and copens) were purchased and two local feeds (corresponding to treatment) were compounded to contain the same level of crude protein. Locally ingredients used are maize, fish meal, groundnut cake, soya bean meal, bone meal, lysine, methionine, premix, vegetable oil, salt but varying fish meal and soya bean meal as follows, treatment 1- (11.38% to 7.5%), treatment 2-(7.5% to 11.38%) respectively. The pelleted feeds were fed at the rate of 5% of the fish biomass. The growth response was assessed in terms of daily weight gain, relative weight gain, specific growth rate, feed conversion ratio, mortality and survival rate. The results showed that the two local feeds promoted positive growth, implying that the use of different levels fish meal and soya bean meal (11.38% and 7.5%) favoured culture of juvenile C. gariepinus.Key Words, Clarias gariepinus, diet, fish feed, formulation, growth


1989 ◽  
Vol 49 (2) ◽  
pp. 249-265 ◽  
Author(s):  
N. T. Ngongoni ◽  
J. J. Robinson ◽  
R. P. Aitken ◽  
C. Fraser

ABSTRACTIn six experiments carried out on individually penned Finn Dorset ewes estimates were made of the quantities of non-ammonia nitrogen (NAN) reaching the abomasum and truly digested in the small intestine. For experiments 1 and 2 which each involved 33 ewes given a complete diet of milled hay and concentrates supplemented with varying amounts of a good-quality fish meal during late pregnancy, the overall regression estimate for the daily amount of truly digested NAN required for zero N retention (ewe plus conceptus) was 438 mg/kg M0·75 (441 and 434 for experiments 1 and 2 respectively). The coefficients for the efficiency of utilization of increments of truly digested NAN and maternal tissue N for net N accretion in the conceptus were 0·48 (s.e. 0·039) and 0·84 (s.e. 0·127) respectively.In experiment 3, 24 lactating ewes had their diet supplemented with either soya-bean meal or fish meal. There was no effect of protein source on the amounts of NAN reaching the abomasum in ewes fitted with an abomasal cannula or on milk yield and these observations confirmed the unusually high degradability (measured by the polyester bag technique) of the fish-meal protein in the rumen. For experiments 4, 5 and 6 a total of 36 ewes were used to test the efficiency with which the NAN truly digested in the small intestine was used for the synthesis of milk protein. In the absence of data on the possible contribution of body tissue N to milk N the coefficient for the apparent efficiency of utilization of truly digested NAN when the ewes were given a basal diet containing approximately 10 g crude protein per MJ metabolizable energy was 0·63. For those ewes receiving the basal diet supplemented with either soya-bean meal, fish meal or blood meal the coefficients for the efficiency of utilization for the production of milk N of the increments in truly digested NAN supplied by the three protein sources were 0·61, 0·54 and 0·29 respectively. It is suggested that the low coefficient for blood meal may be due to its low content of methionine.


1998 ◽  
Vol 67 (3) ◽  
pp. 503-512 ◽  
Author(s):  
R. W. J. Steen ◽  
S. D. Johnston ◽  
D. J. Kilpatrick ◽  
D. M. B. Chestnutt

AbstractTwo experiments involving 213 finishing lambs were carried out to examine the responses in growth rate and carcass composition to the addition ofmetabolizable energy (ME) to a mixed diet in theform ofeither grass silage concentrate. In the second experiment the performance of lambs offered silage only was also compared at equal intake with the performance of lambs given mixed diets. On average the silages contained 224 g dry matter (DM) per kg; 148 g crude protein (CP) per kg DM; 86 g ammonia-nitrogen per kg total nitrogen and 723 g digestible organic matter per kg DM. Concentrates were based on barley and soya-bean meal and contained 181 g CP per DM. Responses in carcass weight gain when additional ME was added to mixed silagelconcentrate diets in theform ofgrass silage and concentrates were 9·2 and 10·2 (s.e. 0·90) gIM] respectively in experiment 1 and 9·8 and 10-(s.e. 0·33) g/MJ respectively in experiment 2, and responses in energy retention in experiment 2 were 0·34 and 0-(s.e. 0·012) MJ/M] respectively. However carcass gain and energy retention sustained by a silage only diet were only 0·67 and 0·70 respectively of those sustained by a high-concentrate diet at the same ME intake. The diet did not significantly affect body or carcass composition.


1971 ◽  
Vol 25 (2) ◽  
pp. 225-233 ◽  
Author(s):  
E. R. Ørskov ◽  
C. Fraser ◽  
I. McDonald

1. Four sheep were given four diets containing proportions of rolled barley and soya-bean meal varied to provide 10.3, 13.3, 16.1 and 19.9% crude protein in the dietary dry matter; the treatments were given according to a 4 x 4 Latin square design. The mean daily intake was 989 g dry matter. The apparent disappearance of protein, ash, ether extractives and carbohydrate before the abomasum, between the abomasum and terminal ileum and between the terminal ileum and rectum was measured.2. The amount of non-ammonia crude protein (Y1, g/d) disappearing from the small intestine increased with protein intake (X, g/d) according to the equation Y1 = 2.12X – 0.0057X2–83, reaching a maximum when there was about 19% crude protein in the dry matter of the diet.3. The treatments had no significant effects on the disappearance of starch, ether extractives or ash. About 93% of starch disappeared in the rumen and 6% in the small intestine. The total mean daily intake of ether extractives was 21 g; 9 g were added in the rumen, 24 g disappeared from the small intestine and 6 g were excreted in the faeces. The total mean daily intake of ash was 67 g; 26 g were added in the rumen, 37 g disappeared from the small intestine, 9 g from the large intestine and 47 g were excreted in the faeces.


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