Biotype determination of Spanish populations of Bemisia tabaci (Hemiptera: Aleyrodidae)

1997 ◽  
Vol 87 (6) ◽  
pp. 587-593 ◽  
Author(s):  
P. Guirao ◽  
F. Beitia ◽  
J. L. Cenis

AbstractA survey was made to assess the biotype status of populations of the whitefly Bemisia tabaci (Gennadius) in Spain. The study involved observation of the squash silverleaf reaction, analysis of esterase patterns and application of a RAPD-PCR technique. The results obtained by the three methods were fully consistent and showed that the Spanish populations of B. tabaci were composed of two genetic types. One corresponded to populations of the B biotype, found in Tenerife (Canary Islands), Barcelona, Madrid, Malaga and Almeria. The other, showing a unique RAPD and esterase pattern, was found in Majorca (Balearic Islands), Seville, Valencia, Murcia and Almeria, as well as in the Algarve Region of Portugal. RAPD patterns of other populations from the rest of the world used for comparison showed that populations from Arizona (USA), Israel, France, Denmark, Italy, Netherlands and Japan have similar RAPD patterns typical of the B biotype. By contrast, populations from the Iberian Peninsula, Turkey, India, Pakistan and Arizona (A biotype), showed different and unique patterns.

2011 ◽  
Vol 8 (1) ◽  
pp. 1-7
Author(s):  
Purnama Hidayat ◽  
Noor Aidawati ◽  
Sri Hendrastuti Hidayat ◽  
Dewi Sartiami

Indicator Plant and PCR-RAPD for Biotype Determination of Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae).B. tabaci has been known world wide as a major pest and virus vector of horticulture. In Indonesia the presence of B.tabaci was reported since 1980 and its role as virus vector in tomato and chilli pepper has becoming more importantrecently. Genetic diversity of B. tabaci has been well recognized, but very little information available for diversity of B.tabaci in Indonesia. This research was conducted in Bogor, West Java from May 2004 to June 2005. The aim of thisresearch was to initiate basic information regarding genetic diversity of B. tabaci in Indonesia, particularly in Java Island.Whiteflies population collected from different crops, i.e. tomato, broccoli, chill pepper, eggplant, cucumber, soybean, andedamame, was evaluated using silverleaf-induction test, and RAPD-PCR. It was evidenced that only B. tabaci populationfrom broccoli was able to induce silverleaf. Two genetic types of B. tabaci, i.e. biotype B and non B, were identified basedon polymorphism character of DNA. Population from broccoli was belong to biotype B, whereas other populations fromtomato, chill pepper, eggplant, cucumber, soybean, and edamame were belong to biotype non B.


2000 ◽  
Vol 23 (4) ◽  
pp. 781-785 ◽  
Author(s):  
L.H.C. Lima ◽  
D. Návia ◽  
P.W. Inglis ◽  
M.R.V. de Oliveira

In 1991, the poinsettia strain, silverleaf whitefly or B biotype of Bemisia tabaci was detected in Brazil. This variant is a far more serious agricultural pest than the previously prevalent non-B (BR) biotype. The correct identification of B. tabaci is problematic since it is highly polymorphic with extreme plasticity in key morphological characters that vary according to the host. RAPD-PCR was used to survey the B biotype and other biotypes of B. tabaci in Brazil. Whiteflies were collected from cultivated plants and weeds from 57 different localities and on 27 distinct crops. RAPD analyses using two selected 10-mer primers reliably identified the BR biotype and the B biotype of B. tabaci and also differentiated other whitefly species. The presence of the B biotype was confirmed in 20 Brazilian states. The BR and B biotypes of B. tabaci were found to coexist in the whitefly populations of three different localities: Jaboticabal, SP; Rondonópolis and Cuiabá, MT, and Goiânia, GO.


1998 ◽  
Vol 88 (2) ◽  
pp. 213-215 ◽  
Author(s):  
R. L Blackman ◽  
M Cahill

Whiteflies of the Bemisia tabaci (Gennadius) group are a major and increasing problem for agriculture throughout the world. In particular, the appearance and rapid spread of a highly virulent new form (silver-leaf whitefly, or B-biotype; Brown et ah, 1995), and recognition that the problem involves a complex of sibling species, host races and/or biotypes has stimulated intensive multidisciplinary research on this insect in recent years (Gerling & Mayer, 1996).


2017 ◽  
Vol 31 (2) ◽  
pp. 156-162 ◽  
Author(s):  
O. V. Schneider

The article summarizes the main approaches in the definition of business valuation the economic entity. In the process of business valuation, taking into account the risks of financial and economic activities necessary to obtain information on what stage the owner implements the business will receive income. The most difficult task is the impossibility of accurate prediction in determining the level of income and the determination of a discount rate capitalization of future incomes due to the instability of the economy, both in the country and around the world.


2010 ◽  
Vol 41 (No. 1) ◽  
pp. 8-13 ◽  
Author(s):  
K. Karut ◽  
C. Chu C ◽  
T.J. Henneberry ◽  
C. Kazak

The flight activities of adult sweetpotato whitefly, Bemisia tabaci, and leafhoppers were monitored by plastic cup traps at Boğalı, Taşçı, Hacıali, Doğankent and Balcalı in the Çukurova Plain, Turkey, in 2001 and 2003. Activity of B. tabaci, expressed as numbers of adults caught in traps, was low from May to early July in both years. Numbers of B. tabaci caught at Tasçı were higher than at Boğalı from 10 July and 21 August in 2001. Its numbers were also higher at Doğankent than at Hacıali and Balcalı during August of 2003. The numbers of leafhopper adults caught fluctuated greatly in both years. Numbers remained low until late June, followed by gradual increases in July and August at Boğalı and Taşçı in 2001. In contrast, numbers of adults caught were higher at Hacıali, Doğankent and Balcalı from May through July in 2003, followed by lower catches during the remainder of the season. Daily minimum temperatures in July and August were positively correlated with higher trap catches of both B. tabaci and leafhoppers.


2021 ◽  
Vol 13 (2) ◽  
pp. 621
Author(s):  
Hsin Rau ◽  
Mary Deanne M. Lagapa ◽  
Po-Hsun Chen

The number of consumers with green awareness have grown these days and as a result they have turned to purchase eco-friendly products. For this reason, this study aims to propose a method for eco-design based on the anticipatory failure determination method to develop eco-design products. By using eco-design concepts adopted from the World Business Council for Sustainable Development, the process will limit the failures and issues related to environmental impact in product design. The proposed method for eco-design product in this study follows the following procedure. First, we analyze product failure. Second, we propose the determination of the non-green phenomenon of the failure. Thirdly, we integrate the intensified non-green phenomenon to generate non-green hypotheses and fourthly, we eliminate each non-green phenomenon hypothesis by introducing the contradiction matrix of TRIZ for obtaining solutions. Finally, we assess alternative eco-design solutions by evaluation. To verify the practicality of the new procedure, a washing machine is used as an example for illustration.


2005 ◽  
Vol 95 (1) ◽  
pp. 29-35 ◽  
Author(s):  
H. Delatte ◽  
B. Reynaud ◽  
M. Granier ◽  
L. Thornary ◽  
J.M. Lett ◽  
...  

AbstractFollowing the first detection of tomato yellow leaf curl virus (TYLCV) from R=union (700 km east of Madagascar) in 1997 and the upsurge of Bemisia tabaci (Gennadius) on vegetable crops, two genetic types of B. tabaci were distinguished using RAPD–PCR and cytochrome oxidase I (COI) gene sequence comparisons. One type was assigned to biotype B and the other was genetically dissimilar to the populations described elsewhere and was named Ms, after the Mascarenes Archipelago. This new genetic type forms a distinct group that is sister to two other groups, one to which the B biotype is a member and one to which the Q biotype belongs. The Ms biotype is thought to be indigenous to the region as it was also detected in Mauritius, the Seychelles and Madagascar. Both B and Ms populations of B. tabaci induced silverleaf symptoms on Cucurbita sp., and were able to acquire and transmit TYLCV. Taken together these results indicate that the Ms genetic type should be considered a new biotype of B. tabaci.


1995 ◽  
Vol 34 (2) ◽  
pp. 116-116 ◽  
Author(s):  
ROBIN V. GUNNING ◽  
FRANK J. BYRNE ◽  
BARRY D. CONDÉ ◽  
MEGAN I. CONNELLY ◽  
KAYE HERGSTROM ◽  
...  

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