Effect of β-lactoglobulin on plasma retinol and triglyceride concentrations, and fatty acid composition in calves

2001 ◽  
Vol 68 (4) ◽  
pp. 579-586 ◽  
Author(s):  
SHIRO KUSHIBIKI ◽  
KOICHI HODATE ◽  
JUNICHI KURISAKI ◽  
HIROYUKI SHINGU ◽  
YASUKO UEDA ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Plasma Triglyceride ◽  
Control Group ◽  
Long Chain Fatty Acids ◽  
Plasma Triglyceride Concentration ◽  
Whole Milk ◽  
Plasma Retinol ◽  
Β Lactoglobulin ◽  
Retinol Concentration ◽  
Long Chain

β-Lactoglobulin (β-lg) is the main protein of ruminant milk whey. Although β-lg can bind in vitro to a variety of hydrophobic substrates, mainly retinol and long-chain fatty acids, its physiological function is still unknown. In Exp. 1, we investigated the effect of β-lg on the plasma retinol concentration in preruminant calves. Holstein male calves (n = 20) were fed Holstein whole milk at 40 g/kg body weight (BW) plus vitamin A acetate (500,000 i.u.) with or without β-lg (0·4 g/kg BW). The plasma retinol concentration of 10-d-old calves was greater (P<0·05) in the β-lg-fed group than in the control group during the period from 8 to 12 h and at 24 h after the feeding. The postprandial change of plasma retinol in 40-d-old calves fed milk with β-lg was higher (P<0·05) than that in the control calves only at 12 h after the feeding. In Exp. 2, Holstein male calves (n = 18) were used to investigate the effect of β-lg on plasma triglyceride concentration and fatty acid composition. Calves were fed Holstein whole milk at 40 g/kg BW plus milk fat prepared from whole milk at 2 g/kg BW with or without β-lg (0·4 g/kg BW). Plasma triglyceride concentration at age 10 d was higher (P<0·05) in the β-lg-fed group than in the controls during the periods from 1 to 2 h and from 7 to 11 h after the feeding. At age 40 d, plasma triglyceride in the β-lg-fed group was higher (P<0·05) than in the control group only at 9 h. Ratios of palmitic, stearic, and oleic acids to total plasma lipids were higher (P<0·05) in the calves fed β-lg milk than in the control calves at age 10 d. These results suggest that β-lg enhances the intestinal uptake of retinol, triglyceride, and long-chain fatty acids in preruminant calves.

Binding of long chain fatty acids to β-lactoglobulin

Lipids ◽  
1970 ◽  
Vol 5 (4) ◽  
pp. 403-411 ◽  
Author(s):  
Arthur A. Spector ◽  
John E. Fletcher
Keyword(s):  
Fatty Acids ◽  
Long Chain Fatty Acids ◽  
Β Lactoglobulin ◽  
Long Chain ◽  
Chain Fatty Acids

L-carnitine improves egg production in black neck ostriches

2008 ◽  
Vol 48 (10) ◽  
pp. 1341
Author(s):  
A. Hajibabaei ◽  
M. Shivazad ◽  
Sh. Golzar Adabi ◽  
A. Lavaf ◽  
N. Eila
Keyword(s):  
Fatty Acids ◽  
Breeding Season ◽  
Egg Production ◽  
Basal Diet ◽  
Control Group ◽  
Long Chain Fatty Acids ◽  
Egg Weight ◽  
Dietary Effects ◽  
Protein Sparing ◽  
Long Chain

The important functions of L-carnitine are fostering the oxidation of long-chain fatty acids by mitochondria and stimulating protein-sparing action by increasing energy derived from lipids. The present study was conducted to investigate dietary effects of L-carnitine on egg production of breeder ostriches. Ninety black neck ostrich breeder birds (60 females and 30 males) were examined randomly (completely randomised design) within three treatments and five replicates for 7 months in breeding season. A basal diet was formulated and used for the control group (L1), while two levels of L-carnitine, 250 mg/kg and 500 mg/kg, were included in the basal diet for treatments L2 and L3, respectively. The egg production percentage, egg weight and defective eggshell percentage were measured. The supplementary diet with 500 mg/kg L-carnitine increased (P < 0.01) the egg production percentage. Means (±s.e.) of egg production percentage for L1, L2 and L3 were 9.68, 12.95 and 17.13% (±1.08), respectively. L-carnitine had no effect on the egg weight and the defective eggshell percentage. The results suggest that basal diet supplemented with 500 mg/kg L-carnitine can increase the egg production percentage of ostriches.

The major fatty acids in whole milk fat and in a fraction obtained by crystallization from acetone

1969 ◽  
Vol 36 (2) ◽  
pp. 169-175 ◽  
Author(s):  
Sonja Mattsson ◽  
P. Swartling ◽  
R. Nilsson
Keyword(s):  
Fatty Acids ◽  
Milk Fat ◽  
Unsaturated Fatty Acids ◽  
Short Chain Fatty Acids ◽  
Insoluble Fraction ◽  
Long Chain Fatty Acids ◽  
Acetone Insoluble ◽  
Whole Milk ◽  
Long Chain ◽  
Chain Fatty Acids

SummarySummer and winter milk-fat samples from 14 dairies in Sweden were fractionated by crystallization from acetone solution (1:8) at 15 °C. The composition of the major fatty acids of the parent milk fat and of the acetone insoluble fraction were examined by GLC, and the gross triglyceride pattern by TLC on plates of silicic acid treated with silver nitrate.The fatty acid composition of the milk fat was similar to that of milk fat from other countries and varied according to season and also, to a smaller extent, from region to region. Four fractions, representing 33–45, 41–34, 18–14 and 7–6 % of the fat and which contained progressively smaller proportions of saturated acids, were obtained by TLC.The acetone insoluble glyceride (AIG) fraction was characterized by a smaller content of short-chain fatty acids and unsaturated fatty acids, and a larger content of saturated long-chain fatty acids, than the parent milk fat. AIGs from summer milk fat contained a larger proportion of C18 acids and a smaller proportion of C6–C16 acids than AIGs from winter milk fat.Four fractions representing 62–70, 15–8, 16–15 and 7 % of the AIG fraction were obtained by TLC. The distribution of the triglycerides in the AIG fraction differed from that in the parent milk fat, mostly in the relative amounts of glycerides in the 2 most saturated TLC fractions. The seasonal variation was largely confined to these 2 fractions.

Effects of unsaturation of long-chain fatty acids on rumen protozoal engulfment and microbial protein recycling in protozoa in vitro

2019 ◽  
Vol 59 (4) ◽  
pp. 647 ◽  
Author(s):  
Mengzhi Wang ◽  
Yujia Jing ◽  
Yifan Wang ◽  
Shimin Liu ◽  
Jian Gao ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Control Group ◽  
Long Chain Fatty Acids ◽  
Bacterial Cells ◽  
Microbial Protein ◽  
Group D ◽  
Long Chain ◽  
Chain Fatty Acids

The present study investigated the effects of long-chain fatty acids with different degrees of unsaturation on rumen protozoal engulfment and microbial protein recycling by protozoa in vitro. The seven experimental treatments included stearic acid (C18:0, Group A), oleic acid (C18:1, n-9, Group B), linoleic acid (C18:2, n-6, Group C), α-linoleic acid (C18:3, n-3, Group D), arachidonic acid (C20:4, n-6, Group E), eicosapentaenoic acid (C20:5, n-3, Group F) and calcium palmitate (C16:0, control group, G), each being included at 3% (w/w) in the total culture substrate containing starch, xylan, araban, glucan, mannan, cellulose, pectin, lignin, urea and casein. Three goats fitted with rumen cannula were used to provide rumen fluid. The incubation medium was collected for the measurement of engulfing rate of bacteria by protozoa and microbial biomass after 24 h of in vitro incubation. The results showed that the bacterial density of Group D (5.75 × 109 cells/mL) was significantly higher than that of Groups A, B, E, F and G (P &lt; 0.05), but that of the control (Group G) as well as those of Groups A and B were lower than those of Groups D and C (P &lt; 0.05). Similarly, the bacterial protein was the highest in Group D and the lowest in Group G. The number of bacteria engulfed by protozoa per millilitre were the highest in the Control group G (847 × 105 cells/(mL.h)) and the lowest in Group D (392 × 105 cells/(mL.h)). The recycling rate of bacterial cells was lowest in Group D (0.68%) and the recycling time of bacterial cells was the longest (147 h). The quantity of protein recycled was lowest in Group D and highest in Group G, which derived from the number of bacterial cells engulfed. Therefore, it was concluded that the effects of long-chain fatty acids on rumen microbial protein recycling and microbial protein synthesis mainly relate to their degree of unsaturation, with α-linoleic acid possessing a better ability to suppress bacterial-cell (by protozoa) and reduce protein yield.

Postprandial Activation of Coagulant Factor VII by Long-chain Dietary Fatty Acids

1996 ◽  
Vol 76 (03) ◽  
pp. 369-371 ◽  
Author(s):  
T A B Sanders ◽  
G J Miller ◽  
Tamara de Grassi ◽  
Najat Yahia
Keyword(s):  
Fatty Acids ◽  
Dietary Fatty Acids ◽  
Factor Vii ◽  
Fat Intake ◽  
Long Chain Fatty Acids ◽  
Postprandial Lipaemia ◽  
Increased Risk ◽  
Coagulant Activity ◽  
Long Chain Triglycerides ◽  
Long Chain

SummaryFactor VII coagulant activity (FVIIc) is associated with an increased risk of fatal ischaemic heart disease (IHD). Several reports have suggested that dietary fat intake or hypertriglyceridaemia are associated with elevated levels of FVII. This study demonstrates that an intake of long-chain fatty acids sufficient to induce postprandial lipaemia in healthy subjects leads to a substantial elevation in both FVIIc and the concentration of FVII circulating in the activated form. Such an increase in FVIIc could not be induced by medium-chain triglycerides. These results suggest that the consumption of a sufficient amount of long-chain triglycerides to induce postprandial lipaemia induces the activation of FVII.

scholarly journals Differential Effects of Fatty Acid Saturation and Chain Length on NASH in Juvenile Iberian Pigs

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 682-682 ◽  
Author(s):  
Kayla Dillard ◽  
Morgan Coffin ◽  
Gabriella Hernandez ◽  
Victoria Smith ◽  
Catherine Johnson ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Fatty Acid ◽  
Liver Injury ◽  
Olive Oil ◽  
Coconut Oil ◽  
Long Chain Fatty Acids ◽  
Medium Chain ◽  
Long Chain ◽  
Chain Fatty Acids

Abstract Objectives Non-alcoholic fatty liver disease (NAFLD) represents the major cause of pediatric chronic liver pathology in the United States. The objective of this study was to compare the relative effect of inclusion of isocaloric amounts of saturated medium-chain fatty acids (hydrogenated coconut oil), saturated long-chain fatty acids (lard) and unsaturated long-chain fatty acids (olive oil) on endpoints of NAFLD and insulin resistance. Methods Thirty-eight 15-d-old Iberian pigs were fed 1 of 4 diets containing (g/kg body weight × d) 1) control (CON; n = 8): 0 g fructose, 10.5 g fat, and 187 kcal metabolizable energy (ME), 2) lard (LAR; n = 10): 21.6 g fructose, 17.1 g fat (100% lard) and 299 kcal ME, 3) hydrogenated coconut oil (COCO; n = 10): 21.6 g fructose, 16.9 g fat (42.5% lard and 57.5% coconut oil) and 299 kcal ME, and 4) olive oil (OLV, n = 10): 21.6 g fructose, 17.1 g fat (43.5% lard and 56.5% olive oil) and 299 kcal ME, for 9 consecutive weeks. Body weight was recorded every 3 d. Serum markers of liver injury and dyslipidemia were measured on d 60 at 2 h post feeding, with all other serum measures assessed on d 70. Liver tissue was collected on d 70 for histology, triacylglyceride (TG) quantification, and metabolomics analysis. Results Tissue histology indicated the presence of steatosis in LAR, COCO and OLV compared with CON (P ≤ 0.001), with a further increase in in non-alcoholic steatohepatitis (NASH) in OLV and COCO compared with LAR (P ≤ 0.01). Alanine and aspartate aminotransferases were higher in COCO and OLV (P ≤ 0.01) than CON. All treatment groups had lower liver concentrations of methyl donor's choline and betaine versus CON, while bile acids were differentially changed (P ≤ 0.05). COCO had higher levels of TGs with less carbons (Total carbons &lt; 52) than all other groups (P ≤ 0.05). Several long-chain acylcarnitines involved in fat oxidation were higher in OLV versus all other groups (P ≤ 0.05). Conclusions Inclusion of fats enriched in medium-chain saturated and long-chain unsaturated fatty acids in a high-fructose high-fat diet increased liver injury, compared with fats with a long-chain saturated fatty acid profile. Further research is required to investigate the mechanisms causing this difference in physiological response to these dietary fat sources. Funding Sources ARI, AcornSeekers.

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