Effect of different milking intervals on the composition of cisternal and alveolar milk in dairy cows

2004 ◽  
Vol 71 (3) ◽  
pp. 304-310 ◽  
Author(s):  
Moez Ayadi ◽  
Gerardo Caja ◽  
Xavier Such ◽  
Maristela Rovai ◽  
Elena Albanell

Effects of six different milking intervals on the distribution of milk between cistern and alveoli were studied in a randomized, incomplete Latin Square experiment with four lactating Holstein cows. Cisternal and alveolar milk was measured by udder quarter at 4, 8, 12, 16, 20 and 24-h intervals with a 3-d interperiod of regular milking. Cisternal milk was evacuated using a cannula after injection of an oxytocin-receptor blocking agent, followed by an injection of oxytocin to remove the alveolar fraction. Milk samples from each fraction and quarter were collected for analysis. Cisternal and alveolar milk increased with milking interval and represented on average 30 and 70% of the milk stored in the udder, respectively. Fat content in alveolar milk remained constant during the first 16 h, increasing rapidly thereafter, reaching its maximum at 24 h (6·95%). Fat content in cisternal milk decreased with milking interval and reached its minimum at 24 h (0·96%). Total fat yield tended to increase for cisternal milk with longer milking intervals, but it increased markedly for alveolar milk, showing that fat globules did not pass freely from alveoli to cistern between milkings. Milk protein content was greater in rear quarters than in front quarters for both milk fractions. Milk protein content increased in the cisternal milk fraction and tended to increase in the alveolar milk fraction with longer milking intervals, but values did not differ between cisternal and alveolar fractions or between front and rear quarters. Total protein yield increased with milking interval in both fractions, indicating that casein micelles passed more freely than fat globules from the alveolar to the cisternal compartment. In conclusion, the short-term effects of milking intervals in milk composition were explained by the changes observed in alveolar and cisternal milk ratio.

1971 ◽  
Vol 38 (3) ◽  
pp. 287-294 ◽  
Author(s):  
S. Gordin ◽  
R. Volcani ◽  
Yehudith Birk

SummaryThe effects of diets high in concentrates and low in roughage on the yield of milk and milk constituents were studied in 32 cows and heifers throughout lactation. A high level of concentrates in the ration caused an increase in milk yield, a decrease in fat percentage but not in total fat yield, and an increase in protein production.Milk produced under such feeding conditions is capable of yielding greater quantities of curd than would be expected from its fat content, and should be evaluated accordingly. Feed requirements of the cows should be assessed not solely on the basis of the fat content of their milk but also in accordance with the protein content.


Author(s):  
Martin Skýpala ◽  
Gustav Chládek

Milk yield varies during lactation, following what is termed a lactation curve. ŽIŽLAVSKÝ and MIKŠÍK (1988) recorded changes in milk yield within a day, too. TEPLÝ et al. (1979) a KOUŘIMSKÁ et al. (2007) published variation within a day ± 1.10 kg in milk yield, ± 0.75 % in milk fat content and ± 0.20 % in milk protein content. Milk yield of cows can be expressed in many different ways, for instance, in kilograms per lactation or in kilograms per day. A practical parameter describing milk production is milk yield (kg) per milking.The object of experiment were 12 cows of Holstein cattle on the first lactation from the 100-day of lactation to 200-day of lactation. The samples of milk were collected from January to May 2007, once a month from the morning and evening milking (milking interval 12 h ± 15 min.). The following parameters were monitored: milk production – milk yield (kg), milk protein production (kg), milk fat production (kg); milk composition – milk protein content (%), milk fat content (%), lactose content (%), milk solids-not-fat content (%), milk total solids content (%); technological properties of milk – ti­tra­tab­le acidity (SH), active acidity (pH), rennet coagulation time (s), quality of curd (class) and somatic cell count as a parameter of udder health.Highly significant differences were found (P < 0.01) between morning milk yield (15.7 kg) and evening milk yield (13.8 kg), between morning milk protein production (0.51 kg) and evening milk protein production (0.45 kg) and between evening milk fat content (4.41 %) and morning milk fat content (3.95 %). A significant difference (P < 0.05) was found between morning milk total solids content (12.62 %) and evening milk total solids content (12.07 %). No significant differences were found between morning (M) and evening (E) values of the remaining parameters: milk fat production (M 0.62 kg; E 0.60 kg), milk protein content (M 3.24 %; E 3.27 %), milk lactose content (M 4.78 %; E 4.86 %), milk solids-not-fat content (M 7.69 %; E 7.71 %), somatic cell count (M 80 000/1 mL; E 101 000/1 mL), titratable aci­di­ty (M 7.75 SH; E 7.64 SH), active acidity (M pH 6.58; E pH 6.61), rennet coagulation time (M 189 s.; E 191 s.), quality of curd (M 1.60 class; E 1.57 class).


Nativa ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 145
Author(s):  
Maura Frare Menegon ◽  
Claudineli Cassia Bueno da Rosa ◽  
Carmen Wobeto ◽  
Marcia Rodrigues Carvalho Oliveira ◽  
Henrique Melo da Silva ◽  
...  

Objetivou-se avaliar o método de espectroscopia por ultrassom (EU) e o efeito do congelamento do leite nas análises de gordura e proteína do leite de vaca. Cem amostras foram analisadas imediatamente após a coleta em tanque de expansão (in natura), congeladas a -20ºC por 30 dias e novamente analisadas por métodos oficiais (gordura por butirômetro de Gerber e proteína por micro-Kjeldahl) e por EU. Observou-se efeito de interação (P < 0,01) entre o método analítico e congelamento do leite. A EU superestimou (P < 0,01) o teor de gordura e subestimou (P < 0,01) o teor de proteína no leite in natura ou congelado. O congelamento não afetou (P = 0,15) o teor de gordura obtido pelo método oficial, mas reduziu o teor de gordura (P < 0,01) obtido com a EU. O congelamento aumentou (P < 0,01) o teor de proteína obtido pelo método oficial, mas reduziu (P < 0,01) quando obtido por EU. Os teores de gordura e proteína do leite obtidos pelo método de EU são divergentes daqueles obtidos por métodos oficiais, independente da amostra ser in natura ou congelada.Palavras-chave: método analítico; análise química; correlação. EFFICACY OF ULTRASONIC SPECTROSCOPY AND FREEZING IN EVALUATION OF MILK CHEMICAL COMPOSITION ABSTRACT: The objective was to evaluate the ultrasound spectroscopy (US) method and the effect of milk freezing on the cow's milk fat and protein analysis. One hundred samples were collected from one milk tank, immediately analyzed, and frozen at -20ºC for 30 days and analyzed again by official method (fat: Gerber butyrometer, protein: micro-Kjeldahl) and US. It was observed effect of interaction (P < 0.01) between analytical method and milk freezing. Ultrasound spectroscopy overestimated milk fat content and underestimated milk protein content on in natura or frozen. The milk freezing for 30 days did not affect the milk fat content obtained by official method (P = 0.15), but it reduced the milk fat content (P < 0.01) obtained by US. The milk freezing increased (P < 0.01) the milk protein content obtained by official method, but reduced (P <0.01) when obtained from US. The milk fat and protein content obtained by US are different from those obtained by official methods, regardless of the sample is in natura or frozen.Keywords: analytical method; chemical analysis; correlation.


2000 ◽  
Vol 25 ◽  
pp. 175-177 ◽  
Author(s):  
J.K. Margerison ◽  
C.J.C. Phillips

AbstractSuckling following mechanical milking is common practice in organic dairy production systems and in developing countries. The objective of the experimental work was to assess the effect of suckling and suckling frequency following mechanical milking on milk yield, milk composition and somatic cell count. Two experiments were completed using multiparous dairy cows allocated at 3 days post partum to their respective treatment groups. In experiment one, twenty–four multiparous dairy cows were allocated to one of two treatments for 305 days; 12 cows not suckled (NS) and 12 cows, which were suckled twice daily following mechanical milking (S2). Daily milk yield was significantly greater (P<0.05) in suckled cows, NS 8.0, S2 8.9 (sem 0.18) kg/d. Milk fat content was significantly lower (P<0.05) in suckled cows (NS 32.0, S2 30.7 (sem 0.56) g/kg). However, milk protein was not significantly different in suckled cows, NS 29.2, S2 27.6 (sem 10.79) g/kg compared with non suckled cows. Somatic cell count was significantly lower (P<0.05) in suckled cows NS 106, S2 85 (sem 2.85) 000/ml, compared with non suckled cows. In experiment two, thirty-eight cows were allocated one of two treatments for 120 days; 19 cows not suckled (NS) and 19 cows, which were suckled once daily following the afternoon milking (S1). Suckling took place for fifteen minutes daily following machine milking only. The calves were weaned at 6 months of age. Total daily milk yield was significantly greater (P<0.05) in suckled cows, NS 11.7, S1 12.5 (sem 0.04) (kg/d) compared with non suckled cows. The milk fat and protein content were not significantly different in suckled and non suckled cows. Milk fat content NS 33.4, S1 32.9 (sem 0.14) g/kg and milk protein content NS 29.8, S1 30.0 (sem 0.07) g/kg. In conclusion, suckled cows had significantly higher milk yields. Cows suckled twice daily had significantly lower milk fat content. Suckling did not affect milk protein content. Suckling cows twice daily significantly reduced SCC.


2018 ◽  
Vol 61 (2) ◽  
pp. 197-206 ◽  
Author(s):  
Memis Ozdemir ◽  
Sinan Kopuzlu ◽  
Mehmet Topal ◽  
Omer Cevdet Bilgin

Abstract. Many researchers who have studied the relationships between milk protein polymorphisms and some yield traits in dairy cattle have reported incompatible results. In this study, in order to examine the overall relationships between milk protein genes known as major genes (CSN1S1, CSN2, CSN3, and BLG) and some yield traits (daily milk yield, lactation milk yield, fat yield, fat content, protein yield, and protein content), a meta-analysis was performed using some genetic models reported in the results of previous studies on cattle. The results suggest that the relationships of major milk protein genes with other factors should be studied using the codominant genetic model in general. Relationships among some CSN3 genotypes and fat yield, fat content, and protein content, and relationships between some BLG genotypes and daily milk yield, fat content, protein yield, and protein content were significant (P<0.05). No significant (P>0.05) relationships were found between these genotypes and other milk production traits. In addition, no significant (P>0.05) relationships between the CSN1S1 and CSN2 genotypes with the milk production traits examined were observed.


1999 ◽  
Vol 66 (2) ◽  
pp. 177-191 ◽  
Author(s):  
RAMON CASALS ◽  
GERARDO CAJA ◽  
XAVIER SUCH ◽  
CELINA TORRE ◽  
SERGIO CALSAMIGLIA

Forty-eight Manchega dairy ewes were used during a complete lactation in a 2×2 factorial design to determine the effects of supplementing diets with fat (calcium soaps of palm oil fatty acids, CSFA) and rumen undegradable protein (RUP) on milk production and composition. Factors tested were amounts of CSFA (0 or 200 g/kg) and RUP (300 or 450 g/kg crude protein) in the concentrate. RUP was altered by adding a mixture of maize gluten meal and blood meal. Lactation was divided into one nursing period (period 1, weeks 1–4), and three milking periods (periods 2–4, weeks 5–8, 9–14 and 15–21). Concentrates were given at 0–8 kg/d during periods 1 and 2, and at 0–6 kg/d in periods 3 and 4. Ewes grazed rotationally in an Italian rye-grass pasture and received a daily supplement of 0·8 kg vetch–oat hay during period 1, and 0·3 kg lucerne hay during periods 2–4. For the whole lactation, supplemental fat markedly increased milk fat content (+23%) and yield (+16%), and decreased milk protein content (−9%). The positive effect of feeding CSFA on milk fat content was more evident at the beginning of lactation; however, its negative effect on milk protein was more pronounced in late lactation. Supplementary RUP had little effect, increasing milk protein content only in period 3, when the crude protein content of pasture was lower. Milk yield and lamb growth were not affected by dietary treatments. The results indicated that CSFA can be useful for increasing the milk fat content of dairy ewes at pasture, which may help farmers to produce milk reaching the minimum requirements of fat content for the cheese industry.


Author(s):  
Gustav Chládek ◽  
Vladimír Čejna

The freezing point of milk (FPM) is an instant indicator of violated technological quality of raw milk, especially of dilution. FPM can also vary due to numerous effects associated with changes in milk composition and milk characteristics. Beside the effect of season, phase of lactation, breed, milk yield, sub-clinical mastitis etc. the impacts of nutrition and dietary or metabolic disorders are the most significant and the most frequent (GAJDŮŠEK, 2003). FPM is a relatively stable physical characteristic and due to osmotically active elements it ranges from – 0.510 to – 0.535 °C (HANUŠ et al., 2003b). Recently ŠUSTOVÁ (2001) studied the freezing point of milk in pool samples; she observed seasonal changes in FPM of mixed milk and the effect of different diets on FPM values. KOLOŠTA (2003) looked into the effect of grazing season on FPM. HANUŠ et al. (2003a) analysed possible effects of handling of milk components on FPM.The aim of this work was to describe the relationship between FPM and milk components and the impact of breed, number and phase of lactation on FPM. We analysed 328 milk samples in total, out of which 137 samples were of Czech Pied cows and 191 samples of Holstein cows. The effect of number and phase of lactation was evaluated for both breeds together. The greatest coefficients of correlation in total were found between FPM and lactose content (r = 0.600) and solids non fat (r = 0.523). Lower coefficients of correlation were found between FPM and milk fat content (r = 0.235), milk protein content (r = 0.260) and urea concentration (r = 0.256). These coefficients were considerably lower in Holstein cows than in Czech Pied cows. The coefficients of correlation between FPM and number and phase of lactation and somatic cells count were insignificant. The total mean value of FPM was – 0.534 °C. Breed statistically significantly (P<0.01) affected FPM (+0.006 °C in C breed) and milk fat content (+0.19 % in H breed). Breed highly significantly (P<0.001) affected daily milk yield (+4.9 kg milk in H), milk protein content (+0.27 % in C) and solids non fat (+0.37 % in C). On the contrary, breed had no significant effect on lactose content, urea concentration and somatic cells count.Variability of FPM was greater in Czech Pied cows (5.9 %) than in Holstein cows (0.9 %). Number of lactation had no significant effect on FPM (maximum difference between lactations was 0.008 °C). Phase of lactation had no significant effect on FPM either. Our study revealed the fact that FPM was most of all affected by lactose content and solids non fat. However, the decrease of lactose content was compensated by a tendency of mammary glad to keep constant osmotic pressure. As the somatic sells count was low, there was no decline in lactose content during later lactations so no significant decrease of FPM occurred.


2020 ◽  
Vol 52 (11) ◽  
pp. 1191-1201
Author(s):  
Ling Chen ◽  
Rongfu Tian ◽  
Huilin Zhang ◽  
Xiaolin Liu

Abstract NFκB1/p105 is the critical member of the NFκB family which can suppress inflammation, ageing, and cancer when p50/p50 homodimer is formed. Currently, the research about the role of NFκB1/p105 during cow mastitis is limited. Here, we analyzed the correlation of six single-nucleotide variants of the NFκB1 gene with somatic cell count, milk yield, milk fat content, and milk protein content in 547 Chinese Holstein cows, and explored the mRNA expression profiles of the NFκB family and ubiquitin ligases (βTrCP1, βTrCP2, KPC1, KPC2) in LPS-induced bovine mammary epithelial cells (MECs) by transcriptome-Seq. The association analysis showed that cows with SNV2-TT and SNV6-CC in the NFκB1 gene had significantly higher milk protein content (P &lt; 0.05), while cows with SNV5-TT in the NFκB1 gene had significantly lower somatic cell score (SCS), but CC genotype at SNV5 locus was not detected in our Holstein cows. The transcriptome-Seq results demonstrated the mRNA expression of NFκB1 was increased and peaked at 4 h post-induction, while the mRNA expressions of both KPC1 and BCL3 that promote the anti-inflammation function of NFκB1/p105 were decreased in LPS-induced bovine MECs. TNFAIP3, an inhibitor of both degradation and processing of p105 precursor, was markedly increased by more than 3 folds. Furthermore, bta-miR-125b which targets at the 3ʹUTR of TNFAIP3 was reduced by 50%. These results indicated that SNV5-TT of the NFκB1 gene with lower SCS may be an anti-mastitis genotype that could cope with infection more efficiently in Chinese Holstein cows. In addition, the anti-inflammation role of NFκB1/p105 seemed to be inhibited in LPS-induced-bovine MECs because the formation of the p50/p50 homodimer was arrested. This study provides a new perspective to understand the inflammatory mechanism in dairy mastitis.


2015 ◽  
Vol 67 (3) ◽  
pp. 927-934 ◽  
Author(s):  
G.A. Gagliostro ◽  
E.M. Patiño ◽  
M. Sanchez Negrette ◽  
G. Sager ◽  
L. Castelli ◽  
...  

The aim of the study was to examine the changes in milk fatty acid (FA) profile of grazing buffaloes fed either low (L, 276g/d) or high (H, 572g/d) doses of a blend (70:30, wt/wt) of soybean and linseed oils. Fourteen multiparous Mediterranean buffaloes grazing on a native pasture were fed 4 kg/day of a commercial concentrate containing no supplemental oil over a pre-experimental period of ten days. The baseline milk production and composition and milk FA profile were measured over the last three days. After this pre-experimental period the animals received the same concentrate added with either the L or H oil doses for 26 additional days. Milk yield (g/animal/day) did not differ at the start (1776 ± 522 and 1662 ± 291 for L and H, respectively, P<0.622) or at the end of the trial (4590 ± 991 and 4847 ± 447 in L and H, respectively, P<0.543). Baseline milk fat content (g/kg) averaged 77.1 (±20.5) in L and 74.3 (±9.9) in H (P<0.10) and was reduced (P<0.031) to 60.7 (±23.6) and 49.4 (±11.2) (P<0.0031) respectively after L and H with no differences between treatments (P<0.277). Baseline milk protein content (L=43.2 ± 3.4 and H= 44.3 ± 6.9g/kg) increased after oil supplementation (P<0.0001) in both L (73.2 ± 6.0g/kg) and H (68.4 ± 4.9g/kg) without differences between oil doses (P<0.123). Milk fat content of 14:0 decreased after oil supplementation only in the H treatment (5.29 to 4.03, P<0.007) whereas that of 16:0 was reduced (P<0.001) at both L (24.49 to 19.75g/100g FA) and H (25.92 to 19.17g/100g FA) doses. The reduction of total content of 12:0 to 16:0 was higher (P<0.052) in H (32.02 to 23.93g/100g FA) than L (30.17 to 25.45g/100g FA). Vaccenic acid content increased (P<0.001) from 5.70 to 13.24g/100g FA in L and from 5.25 to 16.77 in H, with higher results in the in H treatment (P<0.001). Baseline rumenic acid was sharply increased (P<0.001) in L (1.80 to 4.09g/100g FA, +127%) and H (1.60 to 4.61g/100g FA, +187%) with no differences between L and H (P<0.19). Overall, these results indicate a pronounced improvement in the nutritional value of milk fat from grazing buffaloes fed little amounts (0.276g/day) of a blend of soybean and linseed oils.


Author(s):  
Л.Р. ЗАГИДУЛЛИН ◽  
Р.Р. ШАЙДУЛЛИН ◽  
Т.М. АХМЕТОВ ◽  
С.В. ТЮЛЬКИН ◽  
А.Б. МОСКВИЧЕВА

Изучена взаимосвязь аллельных вариантов генов пролактина и соматотропина с показателями молочной продуктивности коров черно-пестрой породы по 1 и 3 лактации. У первотелок, как и у полновозрастных животных,  наиболее высокий удой (4642 и 6240 кг), количество молочного жира (174,1 и 215,0 кг) и молочного белка (149,0 и 182,5 кг) достигнуты в группе с генотипом PRL АА. По массовой доле жира и белка в молоке коровы с генотипом PRL ВВ достоверно превосходили животных с генотипом PRL АА на 0,12% (Р<0,05) и 0,05% (Р<0,05) соответственно. По гену соматотропина наибольший уровень молочной продуктивности выявлен у коров с генотипом GH LL при достоверном преимуществе над GH VV у первотелок по удою на 439 кг (Р<0,01), выходу молочного жира — на 13,6 кг (Р<0,001), выходу молочного белка — на 15,3 кг (Р<0,01). У полновозрастных коров превышение над остальными группами было только по удою — на 219—548 кг (Р<0,05). Наибольшая жирномолочность характерна животным с генотипом GH VV по 1 лактации — 3,83% и по 3 лактации — 3,82%, а лучшая белковомолочность — коровам, имеющим аллель V гена GH:  3,21 и 3,23% соответственно. Полученные данные свидетельствуют о более высоком уровне молочной продуктивности у коров с генотипом PRL АА и GH LL, но лучшая жирномолочность и белковомолочность отмечены у особей, имеющих в своем генотипе аллели В гена PRL и V гена GH. The interrelation of allelic variants of prolactin and somatotropin genes with indicators of milk productivity of black-and-white cows for the 1st and 3rd lactation was studied. The highest milk yield (4642 and 6240 kg) amount of milk fat (174.1 and 215.0 kg) and milk protein (149.0 and 182.5 kg) were in the group with the PRL AA genotype in first-calf heifers as well as in full-age animals. In terms of the mass fraction of fat and protein of milk, the cows with the PRL BB genotype significantly exceeded animals with the PRL AA genotype by 0.12% (P<0.05) and 0.05% (P<0.05), respectively. According to the somatotropin gene, the highest level of milk productivity was revealed in cows with the GH LL genotype with a significant advantage over GH VV in first-calf heifers in milk yield by 439 kg (P<0.01), milk fat yield by 13.6 kg (P<0.001), milk protein yield by 15.3 kg (P<0.01). The excess over the other groups in full-age cows was only in terms of milk yield that was by 219-548 kg (P<0.05). The highest protein content of milk is a characteristic of animals with the GH VV genotype, 3.83% is for the 1st lactation and 3.82% is for the 3rd lactation, and the best protein content of milk in cows with V allele of the GH gene is 3.21 and 3.23%, respectively. The obtained data  indicated a higher level of milk productivity in cows with the PRL AA and GH LL genotypes but the best fat and milk content was noted in cows with alleles B of the PRL gene and V of the GH gene in their genotype.


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