scholarly journals Activity in vitro of fungal conidia of Duddingtonia flagrans and Monacrosporium thaumasium on Haemonchus contortus infective larvae

2010 ◽  
Vol 85 (2) ◽  
pp. 138-141 ◽  
Author(s):  
A.R. Silva ◽  
J.V. Araújo ◽  
F.R. Braga ◽  
C.D.F. Alves ◽  
L.N. Frassy
Keyword(s):  
Haemonchus Contortus ◽  
Control Group ◽  
Duddingtonia Flagrans ◽  
Control Groups ◽  
Infective Larvae ◽  
Fungal Isolates ◽  
Petri Dishes ◽  
And Control ◽  
Baermann Method

AbstractThe objective of this work was to evaluate the predatory activity of the fungi Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34a) on Haemonchus contortus infective larvae (L3) in two experimental assays (A and B). In assay A, two treatments and one control were formed and kept for 7 days in Petri dishes with 2% water-agar. Each treatment consisted of 1000 H. contortus L3 and 1000 conidia of only one fungal isolate, and the control group consisted of 1000 L3, without fungus, with 10 repetitions per group. In assay B, 1000 conidia of one of the fungal isolates, AC001 or NF34a, were added to coprocultures made from 20 g of faeces collected from sheep naturally infected with H. contortus. At the end of the experiment, the Baermann method was used to count the non-predated larvae of all Petri dishes from treatment and control groups. In assay A, no difference was observed (P>0.05) between the groups treated with AC001 and NF34a fungi. A difference was observed (P < 0.05) between the treated and control groups. The L3 reduction percentages at the end of the experiment were 87.75 and 85.57%, respectively, for the fungal isolates compared to the control group. In assay B, the reduction percentages for conidia of these isolates were 85.82 and 87.32%, respectively. The results obtained show that D. flagrans (AC001) and M. thaumasium (NF34a) were effective in the in vitro control of sheep H. contortus L3 and could be used in the biological control of this nematode.

scholarly journals Fungal Antagonism Assessment of Predatory Species and Producers Metabolites and Their Effectiveness onHaemonchus contortusInfective Larvae

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Manoel Eduardo Silva ◽  
Fabio Ribeiro Braga ◽  
Pedro Mendoza de Gives ◽  
Jair Millán-Orozco ◽  
Miguel Angel Mercado Uriostegui ◽  
...  
Keyword(s):  
Haemonchus Contortus ◽  
Antagonistic Effect ◽  
Nematophagous Fungi ◽  
Duddingtonia Flagrans ◽  
Clonostachys Rosea ◽  
Infective Larvae ◽  
Fungal Isolates ◽  
Show Evidence ◽  
Fungal Antagonism

The objective of this study was to assess antagonism of nematophagous fungi and species producers metabolites and their effectiveness onHaemonchus contortusinfective larvae (L3). Assay A assesses the synergistic, additive, or antagonistic effect on the production of spores of fungal isolates of the speciesDuddingtonia flagrans,Clonostachys rosea,Trichoderma esau, andArthrobotrys musiformis; Assay B evaluates in vitro the effect of intercropping of these isolates grown in 2% water-agar (2% WA) on L3ofH. contortus.D. flagrans(Assay A) produced 5.3 × 106spores and associated withT. esau,A. musiformis, orC. roseareduced its production by 60.37, 45.28, and 49.05%, respectively.T. esauproduced 7.9 × 107conidia and associated withD. flagrans,A. musiformis, orC. roseareduced its production by 39.24, 82.27, and 96.96%, respectively.A. musiformisproduced 7.3 × 109spores and associated withD. flagrans,T. esau, orC. roseareduced its production by 99.98, 99.99, and 99.98%, respectively.C. roseaproduced 7.3 × 108conidia and associated withD. flagrans,T. esau, orA. musiformisreduced its production by 95.20, 96.84, and 93.56%, respectively. These results show evidence of antagonism in the production of spores between predators fungi.

scholarly journals Predatory activity of the fungi Duddingtonia flagrans, Monacrosporium thaumasium, Monacrosporium sinense and Arthrobotrys robusta on Angiostrongylus vasorum first-stage larvae

2009 ◽  
Vol 83 (4) ◽  
pp. 303-308 ◽  
Author(s):  
F.R. Braga ◽  
R.O. Carvalho ◽  
J.M. Araujo ◽  
A.R. Silva ◽  
J.V. Araújo ◽  
...  
Keyword(s):  
Nematophagous Fungi ◽  
Angiostrongylus Vasorum ◽  
Control Group ◽  
Duddingtonia Flagrans ◽  
Nematode Parasites ◽  
Fungal Isolates ◽  
Wild Canids ◽  
Predatory Capacity ◽  
And Control

AbstractAngiostrongylus vasorum is a nematode that parasitizes domestic dogs and wild canids. We compared the predatory capacity of isolates from the predatory fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34), Monacrosporium sinense (SF53) and Arthrobotrys robusta (I31) on first-stage larvae (L1) of A. vasorum under laboratory conditions. L1A. vasorum were plated on 2% water-agar (WA) Petri dishes marked into 4 mm diameter fields with the four grown isolates and a control without fungus. Plates of treated groups contained each 1000 L1A. vasorum and 1000 conidia of the fungal isolates AC001, NF34, SF53 and I31 on 2% WA. Plates of the control group (without fungus) contained only 1000 L1A. vasorum on 2% WA. Ten random fields (4 mm diameter) were examined per plate of treated and control groups, every 24 h for 7 days. Nematophagous fungi were not observed in the control group during the experiment. There was no variation in the predatory capacity among the tested fungal isolates (P>0.05) during the 7 days of the experiment. There was a significant reduction (P < 0.05) of 80.3%, 74.5%, 74.2% and 71.8% in the means of A. vasorum L1 recovered from treatments with isolates AC001, NF34, SF53 and I31, respectively, compared to the control without fungi. In this study, the four isolates of predatory fungi were efficient in the in vitro capture and destruction of A. vasorum L1, confirming previous work on the efficiency of nematophagous fungi in the control of nematode parasites of dogs and as a possible alternative method of biological control.

scholarly journals The Accuracy and Reliability of Tooth Shade Selection Using Different Instrumental Techniques: An In Vitro Study

Sensors ◽  
2021 ◽  
Vol 21 (22) ◽  
pp. 7490
Author(s):  
Nattapong Sirintawat ◽  
Tanyaporn Leelaratrungruang ◽  
Pongsakorn Poovarodom ◽  
Sirichai Kiattavorncharoen ◽  
Parinya Amornsettachai
Keyword(s):  
Intraclass Correlation ◽  
In Vitro Study ◽  
Control Group ◽  
Intraoral Scanner ◽  
Control Groups ◽  
B Values ◽  
Cie Lab ◽  
And Control ◽  
Shade Selection

This study aimed to investigate and compare the reliability and accuracy of tooth shade selection in the model using 30 milled crowns via five methods: (1) digital single-lens reflex (DSLR) camera with twin flash (TF) and polarized filter (DSLR + TF), (2) DSLR camera with a ring flash (RF) and polarized filter (DSLR + RF), (3) smartphone camera with light corrector and polarized filter (SMART), (4) intraoral scanner (IOS), and (5) spectrophotometer (SPEC). These methods were compared with the control group or manufacturer’s shade. The CIE Lab values (L, a, and b values) were obtained from five of the methods to indicate the color of the tooth. Adobe Photoshop was used to generate CIE Lab values from the digital photographs. The reliability was calculated from the intraclass correlation based on two repetitions. The accuracy was calculated from; (a) ΔE calculated by the formula comparing each method to the control group, (b) study and control groups were analyzed by using the Kruskal–Wallis test, and (c) the relationship between study and control groups were calculated using Spearman’s correlation. The reliability of the intraclass correlation of L, a, and b values obtained from the five methods showed satisfactory correlations ranging from 0.732–0.996, 0.887–0.994, and 0.884–0.999, respectively. The ΔE from all groups had statistically significant differences when compared to the border of clinical acceptance (ΔE = 6.8). The ΔE from DSLR + TF, DSLR + RF, SMART, and SPEC were higher than clinical acceptance (ΔE > 6.8), whereas the ΔE from IOS was 5.96 and all of the L, a, and b values were not statistically significantly different from the manufacturer’s shade (p < 0.01). The ΔE of the DSLR + RF group showed the least accuracy (ΔE = 19.98), whereas the ∆E of DSLR + TF, SMART, and SPEC showed similar accuracy ∆E (ΔE = 10.90, 10.57, and 11.57, respectively). The DSLR camera combined with a ring flash system and polarized filter provided the least accuracy. The intraoral scanner provided the highest accuracy. However, tooth shade selection deserves the combination of various techniques and a professional learning curve to establish the most accurate outcome.

scholarly journals Use of oocytes selected by brilliant cresyl blue staining enhances rabbit cloned embryo development in vitro

Zygote ◽  
2019 ◽  
Vol 27 (3) ◽  
pp. 166-172 ◽  
Author(s):  
Linying Jia ◽  
Bo Ding ◽  
Chong Shen ◽  
Shiwei Luo ◽  
Yanru Zhang ◽  
...  
Keyword(s):  
Cell Mass ◽  
Developmental Competence ◽  
Blue Staining ◽  
Control Group ◽  
Control Groups ◽  
Brilliant Cresyl Blue ◽  
Positive Group ◽  
Cresyl Blue ◽  
And Control

SummaryRabbits play an important role in people’s lives due to their high nutritional value and high-quality hair that can be used as raw material for textiles. Furthermore, rabbits are an important animal model for human disease, as genome-edited animals are particularly valuable for studying gene functions and pathogenesis. Somatic cell nuclear transfer (SCNT) is an important technique for producing genome-edited animals and it has great value in saving endangered species and in clone stem cell therapy. However, the low efficiency of SCNT limits its application, with the selection of suitable rabbit oocytes being crucial to its success. In the present study, we collected oocytes from ovarian follicles and stained them with 26 μM brilliant cresyl blue (BCB). We then matured the oocytes in vitro and used them for SCNT. Comparison of the BCB-positive oocytes with BCB-negative oocytes and the control group showed that the BCB-positive group had a significantly higher maturation rate (81.4% vs. 48.9% and 65.3% for the negative and control groups, respectively), cleavage rate (86.6% vs. 67.9% and 77.9%), blastocyst rate (30.5% vs. 12.8% and 19.6%), total number of blastocysts (90±7.5 vs. 65.3±6.3 and 67.5±5.7), and inner cell mass (ICM)/ trophectoderm (TE) index (42.3±4.2 vs. 30.2±2.1 and 33.9±5.1) (P<0.05). The BCB-positive group had a significantly lower apoptosis index (2.1±0.6 vs. 8.2±0.9 and 6.7±1.1 for the negative and control groups, respectively) (P<0.05). These findings demonstrate that BCB-positive oocytes have a higher maturation ability and developmental competence in vitro, indicating that BCB staining is a reliable method for selecting oocytes to enhance the efficiency of SCNT.

scholarly journals Effect of crotamine, a cell-penetrating peptide, on blastocyst production and gene expression of in vitro fertilized bovine embryos

Zygote ◽  
2014 ◽  
Vol 24 (1) ◽  
pp. 48-57 ◽  
Author(s):  
Iana S. Campelo ◽  
Alexsandra F. Pereira ◽  
Agostinho S. Alcântara-Neto ◽  
Natalia G. Canel ◽  
Joanna M.G. Souza-Fabjan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Control Group ◽  
Cell Penetrating Peptide ◽  
Mrna Levels ◽  
Bovine Embryos ◽  
Control Groups ◽  
Cell Penetrating ◽  
A Cell ◽  
And Control

SummaryThe present study investigated the effects of crotamine, a cell-penetrating peptide from rattlesnake venom, at different exposure times and concentrations, on both developmental competence and gene expression (ATP1A1, AQP3, GLUT1 and GLUT3) of in vitro fertilized (IVF) bovine embryos. In Experiment 1, presumptive zygotes were exposed to 0.1 μM crotamine for 6, 12 or 24 h and control groups (vehicle and IVF) were included. In Experiment 2, presumptive zygotes were exposed to 0 (vehicle), 0.1, 1 and 10 μM crotamine for 24 h. Additionally, to visualize crotamine uptake, embryos were exposed to rhodamine B-labelled crotamine and subjected to confocal microscopy. In Experiment 1, no difference (P > 0.05) was observed among different exposure times and control groups for cleavage and blastocyst rates and total cells number per blastocyst. Within each exposure time, mRNA levels were similar (P > 0.05) in embryos cultured with or without crotamine. In Experiment 2, concentrations as high as 10 μM crotamine did not affect (P > 0.05) the blastocyst rate. Crotamine at 0.1 and 10 μM did not alter mRNA levels when compared with the control (P > 0.05). Remarkably, only 1 μM crotamine decreased both ATP1A1 and AQP3 expression levels relative to the control group (P < 0.05). Also, it was possible to visualize the intracellular localization of crotamine. These results indicate that crotamine can translocate intact IVF bovine embryos and its application in the culture medium is possible at concentrations from 0.1–10 μM for 6–24 h.

scholarly journals IVF outcomes after hysteroscopic metroplasty in patients with T- shaped uterus

2019 ◽  
Vol 5 (1) ◽  
Author(s):  
Esra Uyar ◽  
Deniz Usal ◽  
Belgin Selam ◽  
Mehmet Cincik ◽  
Tayfun Bagis
Keyword(s):  
Unexplained Infertility ◽  
Control Group ◽  
Reproductive Outcomes ◽  
Control Groups ◽  
Ivf Outcomes ◽  
Number Of Patients ◽  
Adverse Pregnancy ◽  
And Control ◽  
The Impact

Abstract Background T- shaped uterus may be associated with infertility and adverse pregnancy outcomes. Hysteroscopic metroplasty may improve the reproductivity for these cases. To our knowledge, there is no data in literature about the clinical consequences of in vitro fertilization (IVF) in patients undergoing hysteroscopic metroplasty for T-shaped uterus. The principal objective of the current study is to assess the impact of hysteroscopic metroplasty for T-shaped uterus on the reproductive outcomes of IVF. Methods IVF outcomes of 74 patients who underwent hysteroscopic metroplasty for T- shaped uterus and 148 patients without any uterine abnormalities and with diagnosis of unexplained infertility (control group) were retrospectively analyzed. Results Patients in metroplasty and control groups were comparable with respect to age, BMI, partner’s age and duration of infertility. Number of patients with a history of pregnancy beyond 20 weeks of gestation was significantly lower in the metroplasty group (4.1% vs 18.2%; p < 0.05). Number of previous unsuccessful cycles and percentage of patients with ≥3 unsuccessful IVF cycles (35.1% vs 17.6%; p < 0.05) were significantly higher in the metroplasty group. There were no significant differences in the reproductive outcomes such as the pregnancy rate, clinical pregnancy or live birth rate between the metroplasty and control groups. There were non-significant trends for higher rates of miscarriage (18.8% vs 8%, p > 0.05) and biochemical pregnancy (20.0% vs 10.7%, p > 0.05) in the metroplasty group compared to the control group. Conclusions Reproductive results of the IVF cycles after hysteroscopic correction of T-shaped uterus were comparable to those of the patients without any uterine abnormalities and with diagnosis of unexplained infertility. Hysteroscopic metroplasty may contribute to improved IVF outcomes in patients with T-shaped uterus.

MMP-3-1612 polymorphism - a risk factor for deep venous thrombosis formation

VASA ◽  
2016 ◽  
Vol 45 (3) ◽  
pp. 233-239
Author(s):  
La-Mei Yu ◽  
Nai-Xuan Li ◽  
Yu-Guo Sheng
Keyword(s):  
Risk Factor ◽  
Venous Thrombosis ◽  
Deep Venous Thrombosis ◽  
Allele Frequency ◽  
Rat Model ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Control Groups ◽  
And Control

Abstract. Background: We investigated the association of the 5A/6A polymorphism in the promoter region at -1612 of the matrix metalloproteinase-3 gene (MMP-3-1612) and deep venous thrombosis (DVT). Patients, materials and methods: The distribution of the MMP-3 (-1612 5A/6A) polymorphism in the case and control groups was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Serum MMP-3 level of two groups was detected using enzyme-linked immunosorbent assay (ELISA). HepG2 cells containing MMP-3-1612 recombinant plasmid were cultured in vitro and the MMP-3 level was defined by luminescence intensity of luciferase. A DVT rat model was built. Serum MMP-3 level in the rats’ wounded vein at different time points was detected by ELISA and recorded for investigation of the association between MMP-3 and DVT. Statistical data analysis was conducted with SPSS18.0. Results: On the basis of the observation of MMP-3-1612 genotype frequency and allele frequency in the case and control groups, we identified significantly higher MMP-3-1612 5A allele frequency and higher serum MMP-3 level in the case group than in the control group (both P < 0.05). According to in vitro luciferase measurements, the 5A allele had higher transcriptional activity than the 6A allele. As observed in the rat model, serum MMP-3 level increased with time passing and thrombosis formation after modelling. Conclusions: The MMP-3-1612 5A/6A polymorphism may effect serum MMP-3 level and over-expression of serum MMP-3 level may be a risk factor for DVT formation.

Evaluating the effectiveness of a Mexican strain ofDuddingtonia flagransas a biological control agent against gastrointestinal nematodes in goat faeces

2005 ◽  
Vol 79 (2) ◽  
pp. 151-157 ◽  
Author(s):  
N.F. Ojeda-Robertos ◽  
P. Mendoza-de Gives ◽  
J.F.J. Torres-Acosta ◽  
R.I. Rodríguez-Vivas ◽  
A.J. Aguilar-Caballero
Keyword(s):  
Single Dose ◽  
Live Weight ◽  
Gastrointestinal Nematode ◽  
Treated Group ◽  
Control Group ◽  
Post Inoculation ◽  
Duddingtonia Flagrans ◽  
Infective Larvae ◽  
Nematode Larvae ◽  
Petri Dishes

AbstractThe use ofDuddingtonia flagransin the control of goat nematodes was investigated. Initially, the time of passage of chlamydospores through the digestive tract of goats was evaluated. Two groups of seven parasite-free kids were formed. Group A received a single dose of 3.5×106D. flagranschlamydospores (FTHO-8 strain) per kg of live weight. Group B did not receive any chlamydospores. Faeces were obtained from each kid daily from day 4 prior to inoculation until day 5 post-inoculation (PI) and were placed in Petri dishes containing water agar. Gastrointestinal nematode infective larvae were added to each Petri dish and incubated at 25°C for 7 days. Petri dishes were examined to detect the fungus and trapped nematodes. A second trial evaluated the effect ofD. flagranson the number of gastrointestinal nematode larvae harvested from goat faecal cultures in naturally infected goats. Two groups of seven goats were formed. The treated group received a single dose of 3.5×106D. flagranschlamydospores per kg of liveweight. The control group did not receive any chlamydospores. Faeces were obtained twice daily from each kid. Two faecal cultures were made for each kid. One was incubated for 7 days and the other for 14 days. Gastrointestinal nematode larvae were recovered from each culture and counted. Percentage of larval development reduction was determined using a ratio of larvae/eggs deposited in the control and treated groups.Duddingtonia flagranssurvived the digestive process of goats, and maintained its predatory activity, being observed from 21 to 81 h PI (3 to 4 days). A reduction in the infective larvae population in the treated group compared to the non-treated group was observed in both incubation periods (7 days: 5.3–36.0%; 14 days: 0–52.8%,P>0.05). Although a single inoculation ofD. flagranscan induce a reduction of infective larvae collected from faeces, a different scheme of dosing may be needed to enhance the efficacy ofD. flagransin goats.

148 MICE BLASTOCYST RECONSTRUCTION BY AGGREGATION OF INNER CELL MASS WITH TROPHECTODERM

2013 ◽  
Vol 25 (1) ◽  
pp. 222
Author(s):  
I. P. Emanuelli ◽  
C. P. Godoi ◽  
M. P. M. Mancini ◽  
C. M. Barros ◽  
M. F. G. Nogueira
Keyword(s):  
Present Model ◽  
Inner Cell Mass ◽  
Cell Mass ◽  
Cell Aggregation ◽  
Control Group ◽  
Control Groups ◽  
Chi Square ◽  
Trophectoderm Cells ◽  
And Control

In order to reduce fetal losses of bovine clones the replacement of trophectoderm (TE) by microinjection of immunosurgically isolated inner cell mass (ICM) was proposed. It was reported that those techniques could damage both ICM and TE. An alternative to decrease this problem would be the technique of cell aggregation by approximation. Currently it has been used only in pre-compaction embryos. The aims of this study were to evaluate the effectiveness to aggregate post-compaction embryos and to verify the feasibility of reconstructed blastocysts production by ICM and TE approximation. Mice (Swiss Webster, SW; and C57BL/6/EGFP, EGFP) were superovulated to obtain embryos 2.5 to 3.5 days post-coitus. In both control groups (CG; exp. 1 and 2), whole embryos from 8 cells to early morula stages and without zona pellucida were aggregated. In all experiments, bisection was performed with a blade controlled by micromanipulator to produce two fragments according to the requirements of each experiment (i.e. demi embryos in Exp. 1 and Exp. 2 or TE fragment and whole ICM in Exp. 3). The joined fragments were in vitro cultured in microwell (300 µm diameter and based on well of the well system) filled with 400 µL of KSOMaa, covered with mineral oil by 24 h (incubation 37°C, 5% of CO2 and saturated humidity). Embryonic aggregation rate (AR) was evaluated by detection of a single and cohesive cell mass (CG from Exp. 1 and Exp. 2) or by the presence of a typical morphologically blastocyst (all the remaining groups). Only to validate AR evaluation, 25% of the aggregation attempts were performed with distinct phenotype embryos (EGFP and SW) and they could be tracked under epifluorescence. In Exp. 1, the aggregation by approximation was tested to evaluate embryonic stages: 2 demi-blastocysts (2DB; n = 28), demi-morula and demi-blastocyst (DMDB; n = 20) and control group (CG; n = 25). In Exp. 2, an adhesive agent (phytohemagglutinin; PHA) and fragment increasing were used in the groups: 2DB (n = 24), 4 demi-blastocysts (4DB, that is four half-blastocyst) and CG (n = 22). After aggregation validation (Exp. 1 and Exp. 2), blastocyst reconstruction by the approximation of ICM and TE fragments (with PHA presence) was tested on groups: 1 TE fragment and 1 ICM (TI; n = 48) and 2 TE fragments and 1 ICM (2T1I; n = 17). The rates were analyzed by chi-square and Fisher’s exact tests (significance of 5%). In experiments 1 (3.6; 15.0; and 60.0%, respectively to 2DB, DMDB, and CG) and 2 (8.3; 36.4; and 77.3%, respectively to 2DB, 4DB, and CG), the AR differed among groups with exception of 2DB and DMDB (exp. 1; P > 0.05). In Exp. 3, there was no difference on the AR between TI and 2T1I (27.1 and 29.4%, respectively). Despite the low adhesion potential of the trophectoderm cells, is feasible to produce chimeric embryos by aggregation. We infer that the use of an adhesive agent could increase AR maintaining the contact of the embryonic fragments. The proposed technique was practical and effective for blastocyst reconstruction. Additionally, the present model established in mice is about to be tested in cattle for further validation. Financial support: FAPESP.

scholarly journals Rhabditis spp., in the Espírito Santo, State of Brazil and evaluation of biological control

2019 ◽  
Vol 28 (2) ◽  
pp. 333-337 ◽  
Author(s):  
Samilla Alves Sobral ◽  
Bruna Silva Ferreira ◽  
Caio Colodette Senna ◽  
Carolina Magri Ferraz ◽  
Tiago Facury Moreira ◽  
...  
Keyword(s):  
Biological Control ◽  
Culture Medium ◽  
Southeastern Brazil ◽  
Control Group ◽  
Espírito Santo ◽  
Fungal Isolates ◽  
Petri Dishes ◽  
Espirito Santo

Abstract The objectives of this study were to describe occurrences of Rhabditis spp. causing parasitic otitis in dairy cattle of Gir breed in the state of Espírito Santo, southeastern Brazil, and to evaluate the biological control of this nematode using the nematophagous fungi Duddingtonia flagrans (AC001) and Monacrosporium thaumasium (NF34). After nematode detection and collection, three groups were formed: two groups that were treated, respectively, with the fungal isolates; and a control group, without fungus. The treatments were as follows: (a) Petri dishes containing the culture medium 2% water agar (WA) + 250 nematodes + AC001; (b) Petri dishes containing 2% WA + 250 nematodes + NF34; and (c) Petri dishes containing only 2% WA + 250 nematodes. After seven days at 27 °C the treatments with fungi were able to capture and destroy the nematodes, with percentages of 82.0% (AC001) and 39.0% (NF34) in relation to the control group. The results demonstrate the occurrence of Rhabditis spp. after animals physical examination and that there was efficacy of the in vitro predatory activity of both fungal isolates. Thus, these results are important because they can assist in future in vivo control of this nematode in cattle.

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