Acute-phase responses in cattle infected with hydatid cysts and microbial agents

2014 ◽  
Vol 89 (4) ◽  
pp. 471-479 ◽  
Author(s):  
A. Sevimli ◽  
F.K. Sevimli ◽  
E. Şeker ◽  
A. Ulucan ◽  
H.H. Demirel
Keyword(s):  
Acute Phase ◽  
Microbial Growth ◽  
Common Species ◽  
Control Group ◽  
Infected Cattle ◽  
Hydatid Cysts ◽  
Amyloid A ◽  
Serum Haptoglobin ◽  
Haptoglobin Level ◽  
Microbial Agents

AbstractThe aim of this study was to investigate the effect of hydatid cysts and microbial agents on the acute-phase response in cattle. Twenty-seven cattle with hydatid cysts and eight apparently healthy cattle comprised the study and control groups, respectively. Parasitological, microbiological, histopathological and immunohistochemical examinations of the liver and lungs were undertaken, and 49 of these organs were infected with cysts. In 14 of 31 (45.1%) livers and 10 of 18 (55.5%) lungs microbial growth was observed. The most frequent species occurring in the liver were Staphylococcus aureus, Escherichia coli, Corynebacterium spp. and Campylobacter spp., whereas in the lungs the most common species was Candida spp., followed by Streptococcus spp., Mannheimia haemolytica, Corynebacterium spp., Micrococcus spp. and S. aureus. The concentration of serum interleukin (IL-6) in infected cattle, 455.35 ± 39.68 pg/ml, was significantly higher than that of 83.02 ± 17.87 pg/ml in the control group (P< 0.001). The serum amyloid A (SAA) level of infected cattle was 7.51 ± 0.41 μg/ml, and 4.84 ± 0.51 μg/ml in the control group (P< 0.001). The serum haptoglobin level of infected cattle was found to be 2.08 ± 0.65 ng/ml, while that of the control group was determined as 3.87 ± 0.91 ng/ml (P>0.05). The highest concentrations of IL-6 were detected in serum of the cattle where microbial growth had been detected, followed by cattle infected with bacteria + Trichostrongylus sp. (P< 0.001). Consequently, SAA showed an important increase in the group infected with hydatid cysts, whereas haptoglobin level decreased. It was noticed that IL-6, like SAA, had a significant role in hydatid cyst infection. Therefore IL-6 and SAA appear to be major markers in the detection of infection of cattle with hydatid cysts.

scholarly journals Assessment of Acute-Phase Protein Response Associated with the Different Pathological Forms of Bovine Paratuberculosis

Animals ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1925
Author(s):  
José Espinosa ◽  
Rubén de la Morena ◽  
Julio Benavides ◽  
Carlos García-Pariente ◽  
Miguel Fernández ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Bacterial Load ◽  
Control Group ◽  
Serum Samples ◽  
Focal Lesions ◽  
Amyloid A ◽  
Cell Mediated Immune Response ◽  
Protein Response ◽  
Antibody Levels

In this study, the concentrations of two acute-phase proteins (APPs), haptoglobin (Hp) and serum amyloid A (SAA), were quantitatively assessed in serum samples from cattle naturally infected with paratuberculosis (PTB). APP profiles were compared across 190 animals classified according to the different pathological forms associated with infection: uninfected (n = 59), with focal lesions (n = 73), multifocal lesions (n = 19), and diffuse paucibacillary (n = 11) and diffuse multibacillary lesions (n = 28). Our results showed a significant increase in both APPs in infected animals compared to the control group, with differences depending on the type of lesion. Hp and SAA levels were increased significantly in all infected animals, except in cows with diffuse multibacillary lesions that showed similar values to non-infected animals. The expression pattern of both APPs was similar and negatively correlated with the antibody levels against PTB. These results indicate that the release of Hp and SAA is related to the presence of PTB lesions associated with a high cell-mediated immune response and a lower bacterial load, suggesting that the pro-inflammatory cytokines that are associated with these forms are the main stimulus for their synthesis. These molecules could show some potential to be used as putative biomarkers of PTB infection, particularly for the identification of subclinical animals showing pathological forms related to latency or resistance to the development of advanced lesions.

scholarly journals The effects of water deprivation-induced dehydration on serum acute phase protein concentrations in sheep

2021 ◽  
Vol 71 (4) ◽  
pp. 2515
Author(s):  
C. DINLER AY ◽  
B. ULUTAS
Keyword(s):  
Drinking Water ◽  
Single Dose ◽  
Acute Phase ◽  
Systemic Inflammation ◽  
Water Deprivation ◽  
Control Group ◽  
Phase Reaction ◽  
Blood Concentrations ◽  
Amyloid A ◽  
Ad Libitum

The effects of dehydration on serum acute phase proteins (APPs) concentrations are unknown in sheep. In this study, it was aimed to reveal the effect of dehydration on the blood concentrations of serum amyloid a (SAA), haptoglobin (Hp), ceruloplasmin (Cp) and fibrinogen (Fb) in Kivircik cross-breeds sheep. The animal materials of the study consisted of 20 healthy sheep. They were divided into 4 equal groups: systemic inflammation group (SIG), a single dose of 5 ml Freund’s complete adjuvant (FCA) was administered intramuscularly, drinking water were provided as ad libitum; dehydration group (DEH), a single dose of 5 ml placebo 0.9% NaCl was administered intramuscularly and water was deprived for consecutive 5 days; systemic inflammation+dehydrationgroup (SIG+DEH), a single dose of 5 ml FCA was administered intramuscularly and water was deprived for consecutive 5 days; and the control group (CON), a single dose of 5 ml placebo 0.9% NaCl was administered intramuscularly and drinking water was provided as ad libitum. Also, feed was offered ad libitum throughout the experimental period in all study groups. Blood samples were collected on days 0 (baseline values), 1, 3, 5, and 7 while clinical examinations were performed daily during the study. Significant increases were found in serum Hp, SAA, Cp and plasma Fb concentrations in SIG and SIG+DEH groups. There was a significant increase only in serum Hp concentration over time in the DEH group. In conclusion, this study exhibited that Hp concentration increased as part of an acute phase reaction in water deprivation-induced dehydration in Kivircik cross-breeds sheep.

scholarly journals Acute Phase Response in Enzootic Bovine Leukosis

2018 ◽  
Vol 68 (2) ◽  
pp. 155
Author(s):  
M. GUSEL ◽  
M. TUTUNCU ◽  
H. ALBAYRAK ◽  
E. OZAN ◽  
R. KOC ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Pearson Correlation ◽  
Elisa Test ◽  
Phase Response ◽  
Infected Cattle ◽  
Enzootic Bovine Leukosis ◽  
Amyloid A ◽  
Bovine Leukosis ◽  
Hematologic Examination

In the present study, we evaluated acute phase response by detecting haptoglobin (Hp), serum amyloid A (SAA) and fibrinogen in cattle with enzootic bovine leukosis (EBL). A total of 60 Holstein cattle (≤3 years old), comprisingof 40 cattle infected with BLV, and 20 BLV-free healthy controls were enrolled in this study. Diagnosis of the BLV infection was performed by serology (ELISA and AGID) and PCR techniques. APPs were detected by commercial ELISA test kits using validated standard procedures as instructed. All the BLV-infected cattle were in good general health and had normal respiratory rates, pulse rates, body temperatures. However, 5 cattle had enlarged, hard, painless, movable superficial lymph nodes in infected group. APPs including Hp (p<0.001), fibrinogen (p<0.001), and SAA (p<0.05) concentrations were significantly higher in cattle with EBL compared to BLV-free cattle. On hematologic examination, total leukocyte, lymphocyte and granulocytes concentrations were significantly higher in infected cattle when compared tocontrols. In addition, Hp and SAA (p<0. 001) concentrations were significantly higher in symptomatic cattle than asymptomatic. The Pearson correlation revealed significant associations between APPs and total leukocyte and granulocytes; however, there was no correlation with lymphocyte. In conclusion, the results of this study showed increased acute phase response in BLV infected cattle.

Acute Phase Response in Enzootic Bovine Leukosis

2018 ◽  
Vol 68 (2) ◽  
pp. 155
Author(s):  
M. GUZEL ◽  
M. TUTUNCU ◽  
H. ALBAYRAK ◽  
E. OZAN ◽  
R. KOC ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Response ◽  
Pearson Correlation ◽  
Elisa Test ◽  
Phase Response ◽  
Infected Cattle ◽  
Enzootic Bovine Leukosis ◽  
Amyloid A ◽  
Bovine Leukosis ◽  
Hematologic Examination

In the present study, we evaluated acute phase response by detecting haptoglobin (Hp), serum amyloid A (SAA) and fibrinogen in cattle with enzootic bovine leukosis (EBL). A total of 60 Holstein cattle (≤3 years old), comprising of 40 cattle infected with BLV, and 20 BLV-free healthy controls were enrolled in this study. Diagnosis of the BLV infection was performed by serology (ELISA and AGID) and PCR techniques. APPs were detected by commercial ELISA test kits using validated standard procedures as instructed. All the BLV-infected cattle were in good general health and had normal respiratory rates, pulse rates, body temperatures. However, 5 cattle had enlarged, hard, painless, movable superficial lymph nodes in infected group. APPs including Hp (p<0.001), fibrinogen (p<0.001), and SAA (p<0.05) concentrations were significantly higher in cattle with EBL compared to BLV-free cattle. On hematologic examination, total leukocyte, lymphocyte and granulocytes concentrations were significantly higher In infected cattle when compared to controls. In addition, Hp and SAA (p<0. 001) concentrations were significantly higher in symptomatic cattle than asymptomatic. The Pearson correlation revealed significant associations between APPs and total leukocyte and granulocytes; however, there was no correlation with lymphocyte. In conclusion, the results of this study showed increased acute phase response in BLV infected cattle.

Balloon Angioplasty and Stent Implantation Induce a Vascular Inflammatory Reaction

2002 ◽  
Vol 9 (1) ◽  
pp. 59-66 ◽  
Author(s):  
Martin Schillinger ◽  
Markus Exner ◽  
Wolfgang Mlekusch ◽  
Markus Haumer ◽  
Ramazanali Ahmadi ◽  
...  
Keyword(s):  
Acute Phase ◽  
Balloon Angioplasty ◽  
Stent Implantation ◽  
High Sensitivity ◽  
Control Group ◽  
Acute Phase Reactants ◽  
Amyloid A ◽  
Diagnostic Angiography ◽  
Artery Disease ◽  
Wbc Count

Purpose: To investigate whether peripheral balloon angioplasty with and without stent implantation independently causes an inflammatory vascular response measured by serum acute-phase reactants. Methods: This was a prospective cohort study enrolled 388 consecutive patients (218 men; median age 70 years, interquartile range 59–76) with peripheral artery disease undergoing balloon angioplasty (n = 187), stent implantation (n = 140), and diagnostic angiography (control group, n = 61). C-reactive protein (CRP) measured by standard and high-sensitivity assays, serum amyloid A (SAA), fibrinogen, and white blood cell (WBC) count were obtained at baseline and at 8, 24, and 48 hours postintervention. Polynomial logistic regression analysis was used to assess the independent association of acute-phase reactants and the interventional group. Results: CRP levels measured by both standard and the high-sensitivity assays significantly increased after balloon angioplasty (standard CRP, p = 0.02; high-sensitivity CRP, p = 0.02) and stent implantation (standard CRP, p = 0.004; high-sensitivity CRP, p = 0.008) compared to the control group adjusting for age, sex, duration of fluoroscopy, volume of contrast, and periprocedural complications. SAA values differed only between the stent group and controls (p = 0.05). Fibrinogen and WBCs were not different among the 3 interventional groups. Conclusions: Balloon injury and stent implantation induce a vascular inflammatory response at the dilated vessel segment measurable by serum acute-phase parameters. The standard CRP assay is adequate to quantify acute-phase response in these patients.

scholarly journals Usefulness of Selected Acute-Phase Proteins in the Postsurgical Monitoring of Arthroscopy and Splint Bone Removal in Horses

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2952
Author(s):  
Maciej Przewoźny ◽  
Magdalena Senderska-Płonowska ◽  
Anna Rząsa ◽  
Heliodor Wierzbicki ◽  
Jacek Borkowski ◽  
...  
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Proteinase Inhibitors ◽  
Maximum Level ◽  
Study Groups ◽  
Orthopedic Procedures ◽  
Control Group ◽  
Complicated Case ◽  
Bone Removal ◽  
Serum Haptoglobin

Background: Arthroscopy and splint bone removal are the common orthopedic procedures in horses. Estimation of the dynamics of acute phase proteins in postoperative monitoring seems to be interesting diagnostic approach. The aim of the study was to investigate changes in the concentrations of plasma inflammatory markers—fibrinogen, haptoglobin, and protease inhibitors—following orthopedic surgery in horses. The study involved 114 horses, divided into two study groups undergoing: arthroscopy (41 horses) and splint bone removal (13 horses). The control group consisted of 60 healthy horses. The blood was collected before the surgery and 24, 48, 72 h, 5, 7, 10, 14 and 28 days after the surgery. Plasma fibrinogen, serum haptoglobin and proteinase inhibitors were measured. Results: In non-complicated cases of arthroscopy and splint bone removal, fibrinogen and haptoglobin increased stepwise from 24 h, achieved the maximum level at 72 h and returned to preoperative levels after 10–14 days. In one complicated case after arthroscopy surgery the marked increase in fibrinogen and haptoglobin concentrations was observed 24 h earlier than standard parameters of inflammation Conclusion: The study shows the evolution of APPs after arthroscopy and splint bone removal in 28 days postsurgery period and in the case of one complicated case of arthroscopy.

scholarly journals The effect of staphylococcal mastitis including resistant strains on serum procalcitonin, neopterin, acute phase response and stress biomarkers in Holstein dairy cows

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11511
Author(s):  
Wael El-Deeb ◽  
Mahmoud Fayez ◽  
Naser Alhumam ◽  
Ibrahim Elsohaby ◽  
Sayed A. Quadri ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Acute Phase ◽  
Proinflammatory Cytokines ◽  
Assessment Tool ◽  
Acute Phase Proteins ◽  
Area Under The Curve ◽  
Serum Levels ◽  
Control Group ◽  
Amyloid A ◽  
Holstein Dairy Cows

Staphylococcal mastitis (SM) is a frequent disease in the dairy cattle that is costly to treat. This study aimed to investigate the alterations in the levels of procalcitonin (PCT), neopterin (NPT), haptoglobin (HP), serum amyloid A (SAA), proinflammatory cytokines (IL-1β, IL-8, TNF-α, IF-γ) and oxidative stress (OS) biomarkers in Holstein dairy cows with SM under field conditions. In addition, we also evaluated the role of examined biomarkers in disease pathogenesis and their use as diagnostic biomarkers for the disease in dairy cows. Fifty-three dairy cows with SM, including those with infections caused by Staphylococcus aureus (n = 42) and methicillin resistant S. aureus (MRSA) (n = 11) were selected for this study. In addition, 20 healthy dairy cows were enrolled as a control group. Higher serum levels of PCT, NP, IL-1β, IL-8, TNF-α, IF-γ, HP and SAA and a state of OS was detected in SM group in comparison with the controls. Moreover, the levels of all examined biomarkers in mastitic cows with S. aureus when compared with those infected with MRSA was not significantly different. All examined biomarkers demonstrated a significant degree of discrimination between SM cows and healthy controls (the area under the curve (AUC) ranged from 83.6 for SAA to 100 for PCT). Our study showed that SM in dairy cows was associated with substantial changes in serum PCT, NPT, Acute phase proteins (APPs), proinflammatory cytokines, and OS levels. This study demonstrates that clinical examination in tandem with quantification of PCT, NPT, APPs and cytokines, OS biomarkers could be a useful assessment tool for SM in dairy cows.

scholarly journals Acute phase proteins and biochemical and oxidative stress parameters in Hypoderma spp. infested cattle

2018 ◽  
Vol 68 (4) ◽  
pp. 535 ◽  
Author(s):  
O. MERHAN ◽  
K. BOZUKLUHAN ◽  
H. I. GOKCE
Keyword(s):  
Oxidative Stress ◽  
Acute Phase ◽  
Whole Blood ◽  
Total Protein ◽  
Acute Phase Proteins ◽  
Control Group ◽  
Blood Samples ◽  
Protein Levels ◽  
Amyloid A ◽  
And Oxidative Stress

The aim of the present study was to determine concentrations of acute phase proteins (APP), oxidative stress and some biochemical parameters in naturally infested cattle with Hypoderma spp. For this purpose, 10 clinically healthy cattle as controls and 25 Brown Swiss cattle with Hypoderma spp. were used. Blood samples were collectedto tubes from jugular vein. Parts of blood samples were stored without any process as a whole blood. The serum was separated from the remaining blood samples. The reduced glutathione (GSH) in whole blood and the level of malondialdehyde (MDA), haptoglobin (Hp), ceruloplasmin, serum amyloid A (SAA), AST, GGT, ALP, CK, albumin, urea and total protein levels in serum were colorimetrically determined. The present study indicated that the concentrations of Hp, SAA, ceruloplasmin, AST, GGT, ALP, CK, and MDA were significantly increased, and albumin, total protein, GSH concentrations were significantly decreased in the Hypoderma spp. infested group compared to the control group. Additionally, the increase in serum Hp levels was proportional to the number of Hypoderma spp. and it was statistically significant. In conclusion, the production of APP increased in a response to acute phase response in animals with subcutaneous warbles. Furthermore, liver functions were also shown to be impaired and oxidative stress developed as a result of metabolic products of the parasite in Hypoderma spp. infested cattle.

Comparison of serum procalcitonin, haptoglobin and C-reactive protein levels in goats with Corynebacterium pseudotuberculosis

2018 ◽  
Author(s):  
G. Akgul ◽  
M.B. Akgul ◽  
D. Ozen ◽  
S. Kahya Demýrbýlek
Keyword(s):  
Acute Phase ◽  
Acute Phase Proteins ◽  
Dairy Farm ◽  
Diagnostic Information ◽  
Control Group ◽  
C Reactive Protein ◽  
Protein Levels ◽  
Reactive Protein ◽  
Serum Haptoglobin ◽  
Significant Difference

This study was conducted to investigate the response of acute phase proteins, mainly Haptoglobin, C-reactive protein, Procalcitonin and the negative acute phase response, especially albumin in goats suffering from C. pseudotuberculosis. A total of 19 Turkish hair goats between the ages of 1.5 and 2 years in a special dairy farm was selected for the study, consisting of 9 healthy animals and 10 with C. pseudotuberculosis. There was a statistically significant difference in serum haptoglobin, C-reactive protein, procalcitonin and albumin levels in goats with C. pseudotuberculosis, compared to the control group (p less than 0.05). The results showed that Haptoglobin, C-reactive protein and Procalcitonin produce a higher, and Alb a lower, response in goats with C. pseudotuberculosis compared to the control. The presented study suggests that C. pseudotuberculosis can influence the level of acute phase proteins in goats. These results indicate that monitoring a number of acute phase proteins can increase the diagnostic information available for this disease.

The serum protein electrophoretic pattern and acute phase protein concentrations in calves with chronic respiratory diseases

2013 ◽  
Vol 16 (2) ◽  
pp. 385-386 ◽  
Author(s):  
Cs. Tóthová ◽  
O. Nagy ◽  
G. Kováč
Keyword(s):  
Serum Protein ◽  
Acute Phase ◽  
Respiratory Diseases ◽  
Serum Amyloid A ◽  
Electrophoretic Pattern ◽  
Serum Amyloid ◽  
Control Group ◽  
Chronic Respiratory Diseases ◽  
Amyloid A ◽  
Protein Electrophoretic Pattern

Abstract The aim of this study was to evaluate the serum protein electrophoretic pattern and the concentrations of acute phase proteins (haptoglobin, serum amyloid A, and fibrinogen) in 28 calves with clinical signs of chronic respiratory diseases and 36 healthy calves as a control group. In sick calves we found significantly higher serum concentrations of total proteins (P<0.001), lower concentrations of albumin (P<0.001) and marked shift in the concentrations of the most of protein fractions with significantly higher values of α1-, β1-, β2-, and γ-globulins (P<0.001 and P<0.01). The affected calves had significantly higher values of haptoglobin, serum amyloid A, and fibrinogen as well (P<0.05, P<0.001 and P<0.001, respectively).

Sign in / Sign up

Export Citation Format

Share Document