scholarly journals Investigations on the incidence of rinderpest virus infection in game animals of N. Tanganyika and S. Kenya 1960/63

1967 ◽  
Vol 65 (3) ◽  
pp. 343-358 ◽  
Author(s):  
W. Plowright ◽  
B. McCulloch
Keyword(s):  
Clinical Signs ◽  
Neutralizing Antibody ◽  
Low Grade ◽  
Serum Samples ◽  
Before And After ◽  
Antibody Titres ◽  
Low Incidence ◽  
Connochaetes Taurinus ◽  
Low Rate ◽  
Game Animals

The incidence of rinderpest infection in game animals in selected localities of South Kenya and North Tanganyika was studied during the period 1960 to 1963. Serum samples from 590 wildebeest (Connochaetes taurinus), 48 eland (Taurotragus oryx), 65 Thompson's gazelle (Gazella thompsoni) and 39 Grant's gazelle (Gazella granti) were tested for rinderpest neutralizing antibody.Rinderpest infection was shown to have been very frequent in yearling wilde-beest in the Mara area of Kenya in 1959/60, in the Serengeti National Park of Tanganyika in late 1960 and also in the Serengeti, and some adjacent areas, during the latter half of 1961. In the Ngorongoro Crater in 1961 infection was far less widespread, with only 11% of the yearlings acquiring antibody, compared to 67% in the Serengeti. The infections in 1959 and 1960 were clinical epizootics, accompanied by a considerable mortality, whereas no overt disease was reported in the course of 1961. Eland were affected in a similar manner to wildebeest up to 1960 but only a low rate of serological conversion was demonstrated in 1961. Adult Thompson's gazelle showed a low rate (ca. 12%) of infection but no anti-body was detected in Grant's gazelle.Only a small proportion of the wildebeest calves born in early 1962 acquired antibody by mid-1963 and this was due, at least in part, to infection late in 1962; it was not clear, unfortunately, whether the positive animals belonged entirely to resident, as opposed to migratory, groups. No clinical signs or mortality were reported in this year.A low incidence of rinderpest infection in wildebeest was also demonstrated both before and after 1960 in the Kajiado district of Kenya, where disease of game has not been reported in recent years. It is possible that the positive animals, as also the 1962 cases in Tanganyika, acquired the virus from low-grade infections of cattle.The transmission of rinderpest antibody from wildebeest dam to calf, presumably via the colostrum, was demonstrated regularly, except in six calves about 1–2 weeks old. No completely satisfactory explanation was obtained for their failure to acquire passive antibody but it may have been due to abnormal disturbance in the herds, associated with the shooting. The antibody titres in calves were initially higher than those in the serum of their dams but by the end of the 3rd month this position had been reversed. Individual calves became serologically negative from about the 10th week of life and all were devoid of antibody by the 6th to 7th month. The half-life of passively-acquired antibody was 4·4 weeks.

scholarly journals Susceptibility of various animals to the vesiculoviruses Isfahan and Chandipura

1986 ◽  
Vol 97 (2) ◽  
pp. 359-368 ◽  
Author(s):  
C. R. Wilks ◽  
J. A. House
Keyword(s):  
Clinical Signs ◽  
Neutralizing Antibody ◽  
Post Inoculation ◽  
Serum Samples ◽  
Pathogenic Potential ◽  
Adult Mice ◽  
Antibody Titres ◽  
The Right ◽  
Chicken Eggs ◽  
Old Mice

SummaryTo determine the pathogenic potential of the vesiculoviruses Isfahan Chandipura for domestic animals, two ponies, two steers, three and three pigs were inoculated with each virus intradermally in the tongue or, in the case of the pigs, in the snout, heel and coronary band The Ponies were also inoculated intradcrmally in the right commissure o the mouth Animals inoculated with each virus were housed in one room and allowed to mingle freely with an equal number of uninoculated contact animals of each species.Clinical signs of infection, consisting of ulcers at the inoculation sites, were observed in the Chandipura study in two inoculated ponies, one inoculated steer and one inoculated goat. No elevated temperature was observed. Virus was isolated from the ulcerated tongue tissue but not from serial blood samples, oesophagcal-pharyngeal mucus samples, or from the tissues which were collected at necropsy. Precipitating antibody was not detected by the immunoelectro osmophoresis(IEOP) test in any of the pre- or post-serum samples except from two inoculated sheep at 29 days post-inoculation (D.P.I.). Low levels of neutralizing activity were dtedted in pre-inoculation serum from all steers, pigs, contact sheep, and one contact goa. By 15 D.P.I. all inoculated animals and contact ponies and steers exhibited increased neutralizing antibody titres.In studies with the Isfahan virus, lesions developed only at the inoculation sites in the two ponies, and the virus was isolated. No virus was isolated from any blood, oesophageal-pharyngeal mucus samples or tisues collected at necropsy. All pre-inoculation sera were negative for neutralizing and precipitating antibodies. By 14 D.P.I. all inoculated animals exhibited neutralizing antibody, while all the contacts remained negative. The IEOP test remained negative for all animals throughout the experiment. A subpassage of a suspension of Isfahan-infected tongue tissue injected in to ponies and steers also yielded only firm swellings of lesser extent than the original reaction at the inoculation sites.With both viruses, lethal infections were produced by intraacranial or intraperitoneal inoculation of day-old mice and hamsters, and by allantoic inoculation of embryonating chicken eggs. Adult mice, hamsters, guinea-pigs and rabbits produced serum antibodies but lacked clinical signs.

scholarly journals Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA

1992 ◽  
Vol 34 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Eide Dias Camargo ◽  
Paulo Mutuko Nakamura ◽  
Adelaide José Vaz ◽  
Marcos Vinícius da Silva ◽  
Pedro Paulo Chieffi ◽  
...  
Keyword(s):  
Low Cost ◽  
Clinical Signs ◽  
Toxocara Canis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Specific Antibodies ◽  
Normal Individuals ◽  
Before And After ◽  
Dot Elisa ◽  
Stability Time

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

scholarly journals Severe acute respiratory syndrome vaccine efficacy in ferrets: whole killed virus and adenovirus-vectored vaccines

2008 ◽  
Vol 89 (9) ◽  
pp. 2136-2146 ◽  
Author(s):  
Raymond H. See ◽  
Martin Petric ◽  
David J. Lawrence ◽  
Catherine P. Y. Mok ◽  
Thomas Rowe ◽  
...  
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Respiratory Tract ◽  
Viral Replication ◽  
Lower Respiratory Tract ◽  
Clinical Signs ◽  
Neutralizing Antibody ◽  
Lung Damage ◽  
Upper Respiratory Tract ◽  
Administration Routes ◽  
Antibody Titres

Although the 2003 severe acute respiratory syndrome (SARS) outbreak was controlled, repeated transmission of SARS coronavirus (CoV) over several years makes the development of a SARS vaccine desirable. We performed a comparative evaluation of two SARS vaccines for their ability to protect against live SARS-CoV intranasal challenge in ferrets. Both the whole killed SARS-CoV vaccine (with and without alum) and adenovirus-based vectors encoding the nucleocapsid (N) and spike (S) protein induced neutralizing antibody responses and reduced viral replication and shedding in the upper respiratory tract and progression of virus to the lower respiratory tract. The vaccines also diminished haemorrhage in the thymus and reduced the severity and extent of pneumonia and damage to lung epithelium. However, despite high neutralizing antibody titres, protection was incomplete for all vaccine preparations and administration routes. Our data suggest that a combination of vaccine strategies may be required for effective protection from this pathogen. The ferret may be a good model for SARS-CoV infection because it is the only model that replicates the fever seen in human patients, as well as replicating other SARS disease features including infection by the respiratory route, clinical signs, viral replication in upper and lower respiratory tract and lung damage.

scholarly journals Studies on the epizootiology of rinderpest in blue wildebeest and other game species of Northern Tanzania and Southern Kenya, 1965–7

1967 ◽  
Vol 65 (4) ◽  
pp. 537-545 ◽  
Author(s):  
W. P. Taylor ◽  
R. M. Watson
Keyword(s):  
Neutralization Test ◽  
Subsequent Infection ◽  
Serum Samples ◽  
Game Species ◽  
Present Survey ◽  
Northern Tanzania ◽  
Potential Contact ◽  
Serum Neutralization Test ◽  
Connochaetes Taurinus ◽  
Game Animals

A serum neutralization test was used to determine the incidence of rinderpest antibodies in populations of blue wildebeest (Connochaetes taurinus albojubatus Thomas) occurring in the Serengeti-Mara districts of N. Tanzania and S. Kenya. By correlating the age of animals at the time of shooting with the presence or absence of antibodies it was possible to outline the course of rinderpest in these animals over the period 1961–7.Serengeti migrant wildebeest were extensively infected in 1961, but not in 1962 or any subsequent year. No samples have been collected from this group since mid-1966. Kirawira resident wildebeest were last infected in 1961 according to samples collected in February 1967. Two of 13 animals born into the Mara resident population in late 1961 or early 1962 were found to possess antibodies, together with a high proportion (74%) of animals born in early 1961. This finding was considered indicative of a rinderpest epizootic in this group in the terminal weeks of 1961. No subsequent infection occurred in Mara wildebeest between 1962 and March 1966. Ngorongoro resident wildebeest were infected in the second half of 1962 but have remained free of rinderpest from 1963 until at least April 1966.The findings of the present survey were compared and contrasted with results of a similar study carried out some 2 to 3 years previously.Field strains of bovine rinderpest virus were isolated from the Loliondo district of N. Tanzania in November 1965 and March 1966. Serum samples from buffalo, eland, impala, warthog, and resident wildebeest which inhabited areas adjoining the two outbreaks failed to provide evidence of any recent epizootic in game animals. Circumstantial evidence indicated that the virus could have persisted in the Loliondo area since 1961. Two resident wildebeest born in 1963 and 1964 respectively, were thought to have been infected during a period when the disease went unrecognised. Serengeti migrant wildebeest were not found to have been infected after a period of potential contact with sick cattle in March 1966.

scholarly journals Zika seroprevalence declines and neutralizing antibodies wane in adults following outbreaks in French Polynesia and Fiji

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Alasdair D Henderson ◽  
Maite Aubry ◽  
Mike Kama ◽  
Jessica Vanhomwegen ◽  
Anita Teissier ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Neutralizing Antibody ◽  
French Polynesia ◽  
Significant Decline ◽  
High Seroprevalence ◽  
Zikv Infection ◽  
Cross Sectional ◽  
Before And After ◽  
Antibody Titres

It has been commonly assumed that Zika virus (ZIKV) infection confers long-term protection against reinfection, preventing ZIKV from re-emerging in previously affected areas for several years. However, the long-term immune response to ZIKV following an outbreak remains poorly documented. We compared results from eight serological surveys before and after known ZIKV outbreaks in French Polynesia and Fiji, including cross-sectional and longitudinal studies. We found evidence of a decline in seroprevalence in both countries over a two-year period following first reported ZIKV transmission. This decline was concentrated in adults, while high seroprevalence persisted in children. In the Fiji cohort, there was also a significant decline in neutralizing antibody titres against ZIKV, but not against dengue viruses that circulated during the same period.

Correlation of avian bornavirus-specific antibodies and viral ribonucleic acid shedding with neurological signs and feather-damaging behaviour in psittacine birds

2019 ◽  
Vol 184 (15) ◽  
pp. 476-476 ◽  
Author(s):  
Alexandra Fluck ◽  
Dirk Enderlein ◽  
Anne Piepenbring ◽  
Ursula Heffels-Redmann ◽  
Sybille Herzog ◽  
...  
Keyword(s):  
Clinical Signs ◽  
Control Group ◽  
Clear Correlation ◽  
Serum Samples ◽  
Viral Shedding ◽  
Neurological Signs ◽  
Antibody Titres ◽  
Causative Agents ◽  
First Time ◽  
Proventricular Dilatation Disease

Parrot bornaviruses (PaBV) are the causative agents of proventricular dilatation disease in psittacine birds, but have also been linked to other clinical signs, including behavioural disorders and neurological signs. The aim of this study was to correlate PaBV infection in birds showing feather-damaging behaviour or neurological signs for which no other cause of disease could be identified. Psittacine birds presented to a private practice were divided into three groups: birds with neurological signs (n=28), birds showing feather-damaging behaviour (n=42) and birds presented for routine examinations (n=56). Swabs of crop and cloaca were collected and investigated for the presence of PaBV-RNA using real time RT-PCR. Additionally, serum samples were taken and examined for the presence of anti-PaBV antibodies by immunofluorescence test. PaBV infection was detected in one of the test systems in 40.5 per cent of all birds (n=126) investigated. In the clinically healthy birds (n=56), 19.6 per cent of the birds were positive in at least one of the PaBV tests, compared with 52.38 per cent of the feather-damaging (n=42) and 64.28 per cent of the neurologically diseased birds (n=28). Interestingly, the anti-PaBV antibody titres in birds with neurological signs were highest up to 1:20 480. High antibody titres (up to 1:5120) were also found in the feather-damaging group, whereas the birds of the control group, if PaBV positive, had only very low titres. Similarly, the highest viral load was found in the group of the neurologically diseased birds, followed by feather-damaging birds, whereas PaBV-positive birds in the control group demonstrated only low viral RNA shedding. A clear correlation between severity of clinical signs, amount of viral shedding and high levels of antibody titres was observed for most of the neurologically diseased birds and also for few birds with feather-damaging behaviour. For the first time, these results clearly indicate a correlation between PaBV infection and neurological signs in birds without gastrointestinal signs presented to the veterinarian in practice. It also may demonstrate a possible correlation with feather-damaging behaviour and anti-PaBV antibody presence. The antibody titre seems to represent a diagnostic tool to correlate clinical signs to PaBV as a cause.

scholarly journals Neutralizing Antibodies against Human Papillomavirus Types 16, 18, 31, 52, and 58 in Serum Samples from Women in Japan with Low-Grade Cervical Intraepithelial Neoplasia

2008 ◽  
Vol 15 (10) ◽  
pp. 1536-1540 ◽  
Author(s):  
Hiroyuki Ochi ◽  
Kazunari Kondo ◽  
Koji Matsumoto ◽  
Akinori Oki ◽  
Toshiharu Yasugi ◽  
...  
Keyword(s):  
Cervical Intraepithelial Neoplasia ◽  
Neutralizing Antibodies ◽  
Neutralizing Antibody ◽  
Intraepithelial Neoplasia ◽  
Human Papillomaviruses ◽  
Blood Collection ◽  
Low Grade ◽  
Limited Information ◽  
Serum Samples ◽  
Hpv Dna

ABSTRACT We have very limited information on serum neutralizing antibody in women naturally infected with the human papillomaviruses (HPVs) that are causally associated with cervical cancer. In this study, serum samples collected from 217 Japanese women with low-grade cervical intraepithelial neoplasia were examined for their neutralizing activities against HPV16, -18, -31, -52, and -58 pseudovirions. Eighty-four patients (39%), 35 patients (16%), 17 patients (8%), and 1 patient were positive for neutralizing antibodies against one, two, three, and four of these types, respectively. Presence of neutralizing antibody did not always correlate with detection of HPV DNA in cervical swabs collected at the time of blood collection. The neutralizing titers of the majority of sera, ranging between 40 and 640, were found to be conserved in the second sera, collected 24 months later, independently of emergence of HPV DNA in the second cervical swabs. The data strongly suggest that HPV infection induces anti-HPV neutralizing antibody at low levels, which are maintained for a long period of time.

scholarly journals The Pathology of Vaccination of Chickens with Varying Doses of Lentogenic LaSota Strain of Newcastle Disease Virus

2021 ◽  
Vol 41 (1) ◽  
pp. 62-72
Author(s):  
A.O. Igwe ◽  
M.E. Sanda ◽  
U.E.I. Nnsewo ◽  
C.J. Okonkwo ◽  
O. Onyebgula
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Broiler Chickens ◽  
Haemagglutination Inhibition ◽  
Clinical Signs ◽  
Lymphoid Hyperplasia ◽  
Post Inoculation ◽  
Serum Samples ◽  
Antibody Titres

Recently, it was demonstrated under laboratory conditions that increased doses of LaSota vaccine increased ND antibody response significantly in chickens. In this study, we have used the same model to investigate whether vaccination with increased doses of  lentogenic LaSota strain of Newcastle disease virus are associated with pathological changes in chickens. Four-week-old broiler chickens (n=100) were randomly assigned into four groups of 25 each: ZD, each drenched with phosphate-buffered saline, SD, DD and TD broilers were each drenched with single, double and triple dose of LaSota vaccine, respectively. The chickens were observed for clinical signs and lesions. Serum samples were collected from the chickens in all the groups at weekly intervals post inoculation (PV) and assayed for haemagglutination inhibition (HI) antibodies. The vaccinated broilers showed no morbidity and mortality. Only the bursa of all the vaccinated groups appeared slightly reduced in size on day 10 PV. The histopathological changes were lymphoid  hyperplasia and formation of germinal centres in the spleen and caecal tonsils from days 3 to 6 PV and mild depletion of bursal lymphocytes on day 10 PV. Generally, the integrity of the lymphoid organs was intact. Groups DD and TD antibody titres were  significantly (P < 0.05) higher than that of the SD on day 21 PV. This suggests that increased doses of LaSota vaccine does not cause  pathologic impairment and may be considered in improving the performance of the vaccine in the control of velogenic ND. Key words: Newcastle disease, LaSota vaccine, pathology, broiler chickens

scholarly journals Comparing Five SARS-CoV-2 Antibody Assay Results Before and After the First and Second ChAdOx1 nCoV-19 Vaccination Among Health Care Workers: A Prospective Multicenter Study

2021 ◽  
Author(s):  
Seri Jeong ◽  
Nuri Lee ◽  
Su Kyung Lee ◽  
Eun-Jung Cho ◽  
Jungwon Hyun ◽  
...  
Keyword(s):  
Health Care Workers ◽  
Adverse Reactions ◽  
Neutralizing Antibody ◽  
Fold Increase ◽  
Serum Samples ◽  
Prospective Multicenter Study ◽  
Care Workers ◽  
Before And After ◽  
Antibody Assays ◽  
Antibody Titers

Reliable results for serologic positivity to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) antibody after the second dose of AstraZeneca (AZ) vaccination are important to estimate the real efficacy of vaccination. We evaluated the positivity rates and the changes of semi-quantitative antibody titers before and after the first and second ChAdOx1 nCoV-19 Vaccinations using five SARS-CoV-2 antibody assays, including two surrogate virus neutralization tests. A total of 674 serum samples were obtained from 228 participants during three blood sampling periods. A questionnaire on symptoms, severity and adverse reactions duration was completed after the second vaccination. The overall positive rates for all assays were 0.0-0.9% before vaccination, 66.2-92.5% after the first vaccination, and 98.2-100.0% after the second vaccination. Median antibody titers in five assays after the second dose of vaccination were increased compared to those after the first dose (106.4-fold increase for Roche total antibody, 3.6-fold for Abbott IgG, 3.6-fold for Siemens, 1.2-fold for SD Biosensor V1 neutralizing antibody, and 2.2-fold for GenScript neutralizing antibody). Adverse reactions reduced after the second dose in 89.9% of participants compared to after the first dose. Overall, the second vaccination led to almost 100% positivity rates based on these SARS-CoV-2 antibody assays. The results should be interpreted with caution, considering the characteristics of applied assays. Our findings could inform decisions regarding vaccination and the use of immunoassays, thus, contributing to the SARS-CoV-2 pandemic control.

scholarly journals Immune Response to Verotoxin 1 and 2 in Children withEscherichia ColiO157:H7 Hemorrhagic Colitis and Classic Hemolytic Uremic Syndrome

1995 ◽  
Vol 6 (3) ◽  
pp. 136-140 ◽  
Author(s):  
François Proulx ◽  
Jean P Turgeon ◽  
Gilles Delage ◽  
Hermy Lior ◽  
Lucette Lafleur ◽  
...  
Keyword(s):  
Immune Response ◽  
Hemolytic Uremic Syndrome ◽  
Neutralizing Antibody ◽  
Hemorrhagic Colitis ◽  
Serum Samples ◽  
Fourfold Increase ◽  
Antibody Titres ◽  
Uremic Syndrome ◽  
Antibody Levels ◽  
Positive Immune Response

Objectives: To compare neutralizing antibody titres against verotoxin (vt)-1andvt-2between children with uncomplicated hemorrhagic colitis (hc) and those with classic hemolytic uremic syndrome (hus).vtantibody titres were also compared in children withhcwho received trimethoprim-sulfamethoxazole with those who did not.Design: Prospective study.Setting: Tertiary pediatric hospital.Population Studied: Children withhc(n=41) or classichus(n=12).Interventions: Serum antibodies againstvt-1andvt-2were determined by quantitative neutralization.Main Results: Antibodies were detected in 40% (21 of 53) of serum samples forvt-1and in 100% (53 of 53) of samples forvt-2. A positive immune response, defined as a fourfold increase invtantibody titres or as a single titre of 1/64 or greater, was found in 0% (0 of 12) of patients withhuscompared with 7% (three of 41) of those withhcforvt-1(P=0.4); and in 17% (two of 12) of patients withhuscompared with 22% (nine of 41) of those withhcforvt-2(P=0.3). The rate of seroconversion against eithervt-1orvt-2was comparable in treated and untreated patients with uncomplicatedhc.Conclusions: There was no evidence that neutralizing antibody levels againstvt-1orvt-2in classichusor after antibiotic therapy are substantially different from those in patients with uncomplicatedhc.

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