scholarly journals Recovery of bluetongue virus serogroup from sera collected for a serological survey from apparently healthy cattle, from the Sudan

1986 ◽  
Vol 96 (3) ◽  
pp. 529-533 ◽  
Author(s):  
E. M. E. Abu Elzein
Keyword(s):  
Vero Cell ◽  
Cell Cultures ◽  
Bluetongue Virus ◽  
Complement Fixation ◽  
Serological Survey ◽  
Agar Gel ◽  
Sole Purpose ◽  
Healthy Cattle ◽  
Apparently Healthy

SUMMARYVirus of the bluetongue (BT) serogroup was recovered from 11% of cattle sera collected from apparently healthy animals in Khartoum Province for the sole purpose of screening for BT antibodies. Since these sera did not contain BT antibodies, the donor cattle could have been scored as BT free in the serological survey.Virus was initially isolated in chicken embryos inoculated intravascularly, and was further adapted to Vero cell cultures. Isolates were identified as belonging to the BT serogroup using the agar gel immunodiffusion (AGID) and complement fixation (CF) tests.The results indicated that cattle in the Sudan could harbour BT virus without showing symptoms of the disease. Such an observation necessitates further work to clarify the role of cattle in the epidemiology of BT in the Sudan.

scholarly journals Serological survey of bluetongue virus in sheep from Minas Gerais

2020 ◽  
Vol 40 (4) ◽  
pp. 261-265
Author(s):  
Daniel A. Biihrer ◽  
Adriana S. Albuquerque ◽  
Adriana H.C.N. Romaldini ◽  
Edviges M. Pituco ◽  
Ana Carolina D. Matos ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Bluetongue Virus ◽  
Minas Gerais ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serological Survey ◽  
Kappa Index ◽  
Serum Samples ◽  
Agar Gel ◽  
Perfect Agreement ◽  
Wild Ruminants

ABSTRACT: Bluetongue is an infectious, non-contagious disease that affects domestic and wild ruminants, caused by a virus from the Orbivirus genus, Reoviridae family, transmitted by arthropod vectors of the Culicoides genus. This paper aims to be the first serological survey of bluetongue in sheep from the Meso-regions of Campo das Vertentes and South and Southeast of Minas Gerais. Samples were collected from sheep from different properties. The serum samples were submitted to Agar Gel Immunodiffusion (AGID) and competitive Enzyme-Linked Immunosorbent Assay (cELISA). 303 serum samples were submitted to AGID and cELISA. In these samples, 164 (54.13%) were positive in the AGID technique, and 171 (56.44%) positive in the cELISA technique, with an almost perfect agreement between the techniques (kappa index = 0.887). In all visited properties, positive animals have been found in the herd. Animals acquired from properties of the studied mesoregions were more likely to be positive in IDGA and cELISA tests than animals acquired from properties in other regions of Brazil (p<0.001). These results suggest that bluetongue virus (BTV) is widespread in the mesoregions of Campo das Vertentes and South and Southeast of Minas Gerais.

scholarly journals Apparent false positive histoplasmin latex agglutination tests in patients with tuberculosis

1976 ◽  
Vol 3 (3) ◽  
pp. 306-308
Author(s):  
A F DiSalvo ◽  
D S Corbett
Keyword(s):  
False Positive ◽  
Gunshot Wound ◽  
Complement Fixation ◽  
Latex Agglutination ◽  
Agglutination Test ◽  
Unknown Etiology ◽  
Agar Gel ◽  
Group Iii ◽  
Disease Hospital ◽  
Apparently Healthy

Serum specimens from patients admitted to a respiratory disease hospital were examined by the histoplasmin latex agglutination, the complement fixation, and the agar gel immunodiffusion tests. Of 300 sera examined, 21 (7.0%) gave an apparent false positive reaction at a dilution of 1:16 or greater. Fourteen (66%) of the 21 patients studied has culturally proven tuberculosis. One patient each had a diagnosis of hypertensive cardiovascular disease with congestive heart failure, infection with atypical mycobacteria (Runyon group III), chronic pneumonitis secondary to gunshot wound, and pulmonary abscess of unknown etiology; two had bronchogenic carcinoma; and one serum specimen came from an apparently healthy employee. The results of the histoplasmin latex agglutination test should be interpreted with caution, particularly if only one serological determination has been made and the titer is low.

Immune Electron Microscopy (IEM) of a Canine Adeno-Associated Virus

1974 ◽  
Vol 32 ◽  
pp. 256-257
Author(s):  
Gunter F. Thomas ◽  
M. David Hoggan
Keyword(s):  
Electron Microscopy ◽  
Cell Cultures ◽  
Complement Fixation ◽  
Hepatitis Virus ◽  
Wide Distribution ◽  
Immune Electron Microscopy ◽  
Adeno Associated Virus ◽  
Virus Like Particle ◽  
Dog Kidney ◽  
Green Monkeys

In 1968, Sugimura and Yanagawa described a small 25 nm virus like particle in association with the Matsuda strain of infectious canine hepatitis virus (ICHV). Domoto and Yanagawa showed that this particle was dependent on ICHV for its replication in primary dog kidney cell cultures (PDK) and was resistant to heating at 70°C for 10 min, and concluded that it was a canine adeno-associated virus (CAAV). Later studies by Onuma and Yanagawa compared CAAV with the known human serotypes (AAV 1, 2, 3) and AAV-4, known to be associated with African Green Monkeys. Using the complement fixation (CF) test, they found that CAAV was serologically related to AAV-3 and had wide distribution in the dog population of Japan.

The role of calcium in the increase in flocculence of yeast growing in the presence of copper

1965 ◽  
Vol 162 (989) ◽  
pp. 555-566 ◽  
Keyword(s):  
Liquid Medium ◽  
Cell Cultures ◽  
Chloride Solution ◽  
Liquid Copper ◽  
Acquired Resistance ◽  
Calcium Chloride Solution ◽  
Copper Resistance ◽  
Liquid Cultures ◽  
Selection Of

Growth in the presence of inhibitory concentrations of copper enhances the tendency of yeast to flocculate. Many yeasts will not flocculate unless calcium is included in the growth medium and Guinness strain 522 used in the present work required a relatively large amount. Single cell cultures may undergo variation during subculture, resulting in the production of a large number of variants (Chester 1963). The cells of these variants differ considerably in their ability to adhere together. Flocculation variants of strain 522 differed among themselves in the amount of calcium necessary for flocculation, the most flocculent variants requiring least calcium. Washed cells of the more flocculent yeasts removed more calcium from a calcium chloride solution than did those with lesser powers of adhesion. In a copper medium con­taining calcium the more flocculent variants replaced the less flocculent. Calcium protected cells from copper and the more flocculent variants enjoyed most protection. All variants acquired resistance to copper during growth in the copper medium. Despite the selection of the more flocculent yeasts during growth in liquid medium, their copper resistance was less than that of the less flocculent yeasts. When calcium was added to the liquid copper medium, cultures developed less resistance. It is concluded that the less flocculent cells, having less protection by calcium, were exposed to what was effectively a greater concentration of copper and therefore became more resistant. This greater resistance did not enable these cells to compete with the flocculent cells in liquid cultures.

Possible role of mitochondria in posttetanic potentiation of GABAergic synaptic transmission in rat neocortical cell cultures

Synapse ◽  
2005 ◽  
Vol 58 (1) ◽  
pp. 45-52 ◽  
Author(s):  
Maksim V. Storozhuk ◽  
Svetlana Y. Ivanova ◽  
Pavel M. Balaban ◽  
Platon G. Kostyuk
Keyword(s):  
Synaptic Transmission ◽  
Cell Cultures ◽  
Posttetanic Potentiation ◽  
Gabaergic Synaptic Transmission

Rapid detection of Australia/SH antigen and antibody by a simple and sensitive technique of immunoelectronmicroscopy

1971 ◽  
Vol 17 (7) ◽  
pp. 993-1000 ◽  
Author(s):  
A. E. Kelen ◽  
A. E. Hathaway ◽  
D. A. McLeod
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Practical Method ◽  
Rapid Screening ◽  
Blood Products ◽  
Agar Gel ◽  
Virus Particles ◽  
Antigen Antibody ◽  
Filtration Technique ◽  
Electronmicroscopic Examination

A simple and practical method is presented for demonstrating the presence of the Australia/SH antigen and its corresponding antibody in serum specimens, both qualitatively and quantitatively. The method is based on the electronmicroscopic visualization of characteristic aggregates of antigen–antibody complexes formed in the mixture of a serum specimen and the appropriate Australia/SH detector reagent. It involves the use of a microtechnique requiring minute amounts of reagents and provides, as a result of diffusion and filtration through agar gel, partially purified and concentrated preparations, ready for electronmicroscopic examination in less than an hour. The method is highly specific and yields reproducible results. Its sensitivity was found to be greater than that of the crossover electrophoresis test and closely approximates that of the complement fixation test, with the added advantage of not being affected by the "prozone phenomenon." The method can be recommended for use in laboratories equipped with electronmicroscopic facilities to establish a differential diagnosis of viral hepatitis cases, perform rapid screening of blood samples (blood products) for the presence of Australia/SH antigen, and clarify equivocal results obtained by other methods. It is expected that the agar–diffusion–filtration technique will also prove useful, in general, for enhancing the chances of detecting virus particles in suspensions of relatively low virus concentrations.

Role of Screening Tests in the Detection and Management of Blood Pressure Abnormalities Among Young Population

Angiology ◽  
2016 ◽  
Vol 68 (5) ◽  
pp. 441-446 ◽  
Author(s):  
Mehmet Karaduman ◽  
Mustafa Aparci ◽  
Murat Unlu ◽  
Cengiz Ozturk ◽  
Sevket Balta ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Cardiovascular Disease ◽  
Body Mass Index ◽  
Heart Rate ◽  
Medical Records ◽  
Screening Tests ◽  
Young Population ◽  
Males And Females ◽  
Apparently Healthy

The prevalence of hypertension is increasing among young population worldwide. So there is an interest in detecting prehypertension and hypertension in childhood. We determined blood pressure (BP) recorded at a screening test in a young population. We retrospectively evaluated the medical records including systolic blood pressure (SBP), diastolic blood pressure (DBP), weight, height, and body mass index (BMI) of 685 male and 130 female apparently healthy adolescents. We evaluated the prevalence of BP categories: normotension, prehypertension, and hypertension. The prevalence of normotension (n = 369), prehypertension (n = 333), and hypertension (n = 113) was 45.3%, 40.9% and 13.9%, respectively; prehypertension and hypertension were significantly higher among males. The prevalence of prehypertension and hypertension was significantly higher among overweight males and females. The BMI significantly correlated with SBP, DBP, and heart rate among both genders. The prevalence of prehypertension and hypertension is high among Turkish adolescents. Screening tests focused on BP and BMI measurement may help detect the young population at risk of hypertension and cardiovascular disease in the future.

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