Symbiont composition of the basidiolichen Lichenomphalia meridionalis varies with altitude in the Iberian Peninsula

AbstractBasidiolichens are generally poorly researched because of the very small number of species and their restriction to special niches. Lichenomphalia basidiolichens grow in considerable quantities in arctic and alpine habitats but they are inadequately studied in these habitats in Mediterranean areas. Based on morphological and phylogenetic analyses, we identified the different symbionts of L. meridionalis, collected in localities in Spain at altitudes ranging from 533 to 2200 m above sea level. The present study provides the first molecular data available for L. meridionalis. We found that a microindel of six bp within the nrITS2 could help to discriminate L. meridionalis from other species of the genus. Molecular analyses revealed the existence of two different green algal strains, both belonging to Coccomyxa subellipsoidea, a species shared with other Lichenomphalia lichens. Notably, the two chlorobiont strains associated with L. meridionalis were differentially distributed according to altitude, and samples having one of the two strains consistently also included cyanobacteria.

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Morpho-phylogenetic evidence reveals new species in Rhytismataceae (Rhytismatales, Leotiomycetes, Ascomycota) from Guizhou Province, China

MycoKeys ◽

10.3897/mycokeys.76.58465 ◽

2020 ◽

Vol 76 ◽

pp. 81-106

Author(s):

Jin-Feng Zhang ◽

Jian-Kui Liu ◽

Kevin D. Hyde ◽

Anusha H. Ekanayaka ◽

Zuo-Yi Liu

Keyword(s):

New Species ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Comparative Morphology ◽

Morphological Characters ◽

Molecular Data ◽

Guizhou Province ◽

Molecular Analyses ◽

Phylogenetic Affinities ◽

Karst Terrains

Karst formations represent a unique eco-environment. Research in the microfungi inhabiting this area is limited. During an ongoing survey of ascomycetous microfungi from karst terrains in Guizhou Province, China, we discovered four new species, which are introduced here as Hypoderma paralinderae, Terriera karsti, T. meitanensis and T. sigmoideospora placed in Rhytismataceae, based on phylogenetic analyses and morphological characters. Molecular analyses, based on concatenated LSU-ITS-mtSSU sequence data, were used to infer phylogenetic affinities. Detail descriptions and comprehensive illustrations of these new taxa are provided and relationships with the allied species are discussed, based on comparative morphology and molecular data.

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Cymbidium motuoense (Orchidaceae; Epidendroideae), a new species from China: evidence from morphological and molecular data

Phytotaxa ◽

10.11646/phytotaxa.509.1.5 ◽

2021 ◽

Vol 509 (1) ◽

Author(s):

WENQI HU ◽

QINGHAI ZHANG ◽

GUIZHEN CHEN ◽

MENGJIA ZHU ◽

XIA YU ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Plastid Dna ◽

Molecular Data ◽

Natural Hybrid ◽

Closely Related Species ◽

Orchid Species ◽

Molecular Analyses ◽

Morphological And Molecular Data ◽

A New Species

This study describes a new orchid species, Cymbidium motuoense, from Xizang, China based on morphological and molecular analyses. Several unique morphological features distinguish this new orchid from all other species of Cymbidium. Based on its morphology, this orchid is similar to C. tracyanum but differs in its yellow-green flower with dark purple-red spots on the veins, oblong-elliptic sepals, side lobes of the lip with reddish brown hairs along the veins and disc with three purple-red lamellae. Phylogenetic analyses based on plastid DNA (matK and rbcL) supported C. motuoense as a new species. However, the low support in nuclear ribosomal ITS tree provide the possibility that the new orchid may be a natural hybrid of C. tracyanum and C. iridioides or another closely related species.

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Morphological and molecular characterization of myxobolids (Cnidaria, Myxozoa) infecting cypriniforms (Actinopterygii, Teleostei) endemic to the Iberian Peninsula

Parasite ◽

10.1051/parasite/2019049 ◽

2019 ◽

Vol 26 ◽

pp. 48

Author(s):

Sónia Rocha ◽

Carlos Azevedo ◽

Ângela Alves ◽

Carlos Antunes ◽

Graça Casal

Keyword(s):

Iberian Peninsula ◽

Phylogenetic Analyses ◽

Geographic Location ◽

Host Shift ◽

Molecular Data ◽

Freshwater Ecosystem ◽

Spined Loach ◽

Morphological And Molecular Data ◽

Species Being

The Iberian Peninsula provides a unique freshwater ecosystem for native and endemic cypriniforms to thrive. Despite cypriniforms being hosts to multiple myxobolids worldwide, little research has been performed in this geographic location. In this study, the examination of three Iberian endemic cypriniforms showed that myxosporean richness in the Iberian Peninsula is underestimated, with three new and one known myxobolid species being reported based on morphological and molecular data (SSU). Myxobolus arcasii n. sp. is described from the kidney and gonads of the “bermejuela” Achondrostoma arcasii, M. duriensis n. sp. from the gills of the Northern straight-mouth nase Pseudochondrostoma duriense, and Thelohanellus paludicus n. sp. from the intestine of the Southern Iberian spined-loach Cobitis paludica. Myxobolus pseudodispar Gorbunova, 1936 is further reported from several organs of P. duriense, and from the spleen of A. arcasii. The occurrence of M. pseudodispar in endemic Iberian species reveals that host-shift followed its co-introduction with central European leuciscids into this geographic location. Several other myxobolids originally described from barbels in central Europe have also been reported from the Iberian endemic cypriniform Luciobarbus bocagei. Nonetheless, except for M. musculi, the identification of these myxobolids in L. bocagei is here shown to be dubious and require molecular confirmation. Phylogenetic analyses reveal M. arcasii n. sp. and M. duriensis n. sp. clustering within different lineages of leuciscid-infecting species, showing that myxobolids entered Leuciscidae as hosts multiple times during their evolution. Constituting the first myxobolid reported from the subfamily Cobitinae, Thelohanellus paludicus n. sp. stands alone in the tree topology.

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Morphological, morphometrical and molecular characterisation of Filenchus fungivorus n. sp., a fungivorous nematode from Japan in a most likely polyphyletic genus (Nematoda: Tylenchina)

Nematology ◽

10.1163/138855409x12470437876116 ◽

2010 ◽

Vol 12 (2) ◽

pp. 235-246 ◽

Cited By ~ 7

Author(s):

Wim Bert ◽

Hiroaki Okada ◽

Ines Tavernier ◽

Gaetan Borgonie ◽

Wouter Houthoofd

Keyword(s):

Principal Component Analysis ◽

Phylogenetic Analyses ◽

Principal Component ◽

Molecular Data ◽

Ssu Rdna ◽

Molecular Characterisation ◽

Molecular Analyses ◽

Fungus Culture ◽

Lateral Lines ◽

Short Body

Abstract Filenchus fungivorus n. sp., isolated from autumn bellflower (Gentiana scrabra Bunge) in Japan and maintained on a fungus culture, is described based on LM, SEM and molecular data. The new species is characterised by combination of the following features: two lateral lines, short body (284-331 μm), short stylet (7.0-8.7 μm), amphid aperture associated with characteristic depression, short ovary, undifferentiated spermatheca, post-vulval sac shorter than corresponding body diam. and short (59-80 μm) non-filiform, almost straight tail and absence of males. According to a principal component analysis, a small tail/vulva-anus (1.3-1.8) and V′ ratio have the highest morphometrical power to separate F. fungivorus n. sp. from the remaining analysed Filenchus spp. (with the exception of F. misellus). Molecular analyses based on SSU rDNA placed F. fungivorus n. sp. within a clade that contained Filenchus sp. (heathland, Belgium), F. (Ottolenchus) discrepans, Malenchus andrassyi and F. misellus. However, the position of this clade could not be resolved within the Tylenchomorpha. Although the phylogenetic analyses, confirmed by Shimodaira-Hasegawa tests, significantly rejected the monophyly of Filenchus (P < 0.001), F. fungivorus n. sp. is placed within Filenchus in anticipation of a review of the Tylenchidae.

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Cymbidium shidianense (Orchidaceae: Epidendroideae), a new species from China: evidence from morphology and molecular data

Phytotaxa ◽

10.11646/phytotaxa.399.1.10 ◽

2019 ◽

Vol 399 (1) ◽

pp. 100 ◽

Cited By ~ 5

Author(s):

GUI-ZHEN CHEN ◽

GUO-QIANG ZHANG ◽

JIE HUANG ◽

MENG WANG ◽

WEN-HUI RAO ◽

...

Keyword(s):

New Species ◽

Yunnan Province ◽

Sequence Data ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Orchid Species ◽

Molecular Analyses ◽

Dna Sequence Data ◽

Pale Green ◽

A New Species

A new orchid species, Cymbidium shidianense (Orchidaceae; Cymbidieae), from Yunnan Province, China, is described and illustrated based on morphological and molecular analyses. This new species is similar in morphology to C. cyperifolium, but it differs in its larger pseudobulbs, rigid leaves, broader pale green sepals, petals with darkly coloured veins and set of V-shaped blotches on the lip midlobe. A phylogenetic analyses based on nuclear ribosomal ITS and plastid (matK, rbcL) DNA sequence data support C. shidianense as related to C. cyperifolium.

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Molecular systematics of the subaerial green algal order Trentepohliales: an assessment based on morphological and molecular data

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.63990-0 ◽

2006 ◽

Vol 56 (7) ◽

pp. 1709-1715 ◽

Cited By ~ 32

Author(s):

Juan M. López-Bautista ◽

Fabio Rindi ◽

Michael D. Guiry

Keyword(s):

Phylogenetic Analyses ◽

Morphological Characters ◽

Molecular Data ◽

Small Subunit ◽

Rrna Gene ◽

Small Subunit Rrna ◽

Traditional System ◽

Green Algal ◽

Morphological Criteria ◽

Morphological And Molecular Data

The order Trentepohliales is a widespread group of terrestrial green algae. As currently circumscribed, it includes six genera (Cephaleuros, Phycopeltis, Physolinum, Printzina, Stomatochroon and Trentepohlia), the phylogenetic affinities of which are poorly understood. Sequences for the small subunit rRNA gene (18S rDNA) were obtained for several representatives of the order and phylogenetic analyses based on these sequences were compared with the traditional system of classification based on morphological characters. The results indicate that the morphological criteria used for the circumscription of genera and species of the Trentepohliales do not match phylogenetic patterns. Whereas isolates of Cephaleuros form a well-supported monophyletic clade, Trentepohlia is polyphyletic and strains of Trentepohlia, Phycopeltis, Printzina and Physolinum are mixed together in several different lineages. A reassessment of the generic boundaries and a narrower circumscription of some genera (in particular Trentepohlia) will be necessary. Subcuticular habit, heteromorphic life history and occurrence of zoosporangia in clusters are shown to be phylogenetically significant features, whereas other characters commonly used for separation of species and genera (shape and size of the cells, prostrate/erect habit, substratum colonized) have no phylogenetic relevance.

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Cassytha pubescens and C. glabella (Lauraceae) are not disjunctly distributed between Australia and the Ryukyu Archipelago of Japan - evidence from morphological and molecular data

Australian Systematic Botany ◽

10.1071/sb10040 ◽

2012 ◽

Vol 25 (5) ◽

pp. 364 ◽

Cited By ~ 3

Author(s):

Goro Kokubugata ◽

Koh Nakamura ◽

Paul I. Forster ◽

Gary W. Wilson ◽

Ailsa E. Holland ◽

...

Keyword(s):

Endemic Species ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Western Pacific ◽

Ryukyu Archipelago ◽

Molecular Analyses ◽

Molecular Phylogenetic ◽

Taxonomic Confusion ◽

Morphological And Molecular Data ◽

Pacific Species

Morphological comparisons and molecular phylogenetic analyses were conducted to resolve taxonomic confusion in Cassytha glabella and C. pubescens, both of which were first described from Australia and subsequently considered to be disjunctly distributed between Australia and the Ryukyu Archipelago of Japan. In the morphological comparisons, plants considered as C. pubescens in the Ryukyus differ from C. pubescens in Australia in the presence or absence of hairs on the petals, and those considered as C. glabella in the Ryukyus differ from the C. glabella in Australia in bract and peduncle morphology. The molecular analyses indicated that plants attributed to C. pubescens in the Ryukyus were not closely related to C. pubescens in Australia, and were nested in a clade of populations of a Pan-Western Pacific species C. filiformis. Plants attributed to C. glabella in the Ryukyus were distantly related to C. glabella in Australia. We concluded that plants considered as C. pubescens and C. glabella in the Ryukyus are to be respectively treated as C. filiformis and the Ryukyu endemic species C. pergracilis.

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Linking morphological and molecular sources to disentangle the case of Xylodon australis

Scientific Reports ◽

10.1038/s41598-020-78399-8 ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Javier Fernández-López ◽

M. Teresa Telleria ◽

Margarita Dueñas ◽

Mara Laguna-Castro ◽

Klaus Schliep ◽

...

Keyword(s):

New Zealand ◽

Phylogenetic Analyses ◽

Single Species ◽

R Package ◽

Molecular Data ◽

Historical Collections ◽

Close Relationship ◽

Kinship Relations ◽

History Of ◽

Different Sources

AbstractThe use of different sources of evidence has been recommended in order to conduct species delimitation analyses to solve taxonomic issues. In this study, we use a maximum likelihood framework to combine morphological and molecular traits to study the case of Xylodon australis (Hymenochaetales, Basidiomycota) using the locate.yeti function from the phytools R package. Xylodon australis has been considered a single species distributed across Australia, New Zealand and Patagonia. Multi-locus phylogenetic analyses were conducted to unmask the actual diversity under X. australis as well as the kinship relations respect their relatives. To assess the taxonomic position of each clade, locate.yeti function was used to locate in a molecular phylogeny the X. australis type material for which no molecular data was available using morphological continuous traits. Two different species were distinguished under the X. australis name, one from Australia–New Zealand and other from Patagonia. In addition, a close relationship with Xylodon lenis, a species from the South East of Asia, was confirmed for the Patagonian clade. We discuss the implications of our results for the biogeographical history of this genus and we evaluate the potential of this method to be used with historical collections for which molecular data is not available.

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The Complete DNA Sequence of the Mitochondrial Genome of a “Living Fossil,” the Coelacanth (Latimeria chalumnae)

Genetics ◽

10.1093/genetics/146.3.995 ◽

1997 ◽

Vol 146 (3) ◽

pp. 995-1010 ◽

Cited By ~ 1

Author(s):

Rafael Zardoya ◽

Axel Meyer

Keyword(s):

Mitochondrial Genome ◽

Tandem Repeats ◽

Phylogenetic Analyses ◽

Large Data ◽

Molecular Data ◽

Phylogenetic Position ◽

Data Set ◽

Living Fossil ◽

Latimeria Chalumnae ◽

Relationship Of

The complete nucleotide sequence of the 16,407-bp mitochondrial genome of the coelacanth (Latimeria chalumnae) was determined. The coelacanth mitochondrial genome order is identical to the consensus vertebrate gene order which is also found in all ray-finned fishes, the lungfish, and most tetrapods. Base composition and codon usage also conform to typical vertebrate patterns. The entire mitochondrial genome was PCR-amplified with 24 sets of primers that are expected to amplify homologous regions in other related vertebrate species. Analyses of the control region of the coelacanth mitochondrial genome revealed the existence of four 22-bp tandem repeats close to its 3′ end. The phylogenetic analyses of a large data set combining genes coding for rRNAs, tRNA, and proteins (16,140 characters) confirmed the phylogenetic position of the coelacanth as a lobe-finned fish; it is more closely related to tetrapods than to ray-finned fishes. However, different phylogenetic methods applied to this largest available molecular data set were unable to resolve unambiguously the relationship of the coelacanth to the two other groups of extant lobe-finned fishes, the lungfishes and the tetrapods. Maximum parsimony favored a lungfish/coelacanth or a lungfish/tetrapod sistergroup relationship depending on which transversion:transition weighting is assumed. Neighbor-joining and maximum likelihood supported a lungfish/tetrapod sistergroup relationship.

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Molecular analysis confirms Laurenciella marilzae (Rhodophyta, Rhodomelaceae) in the Mediterranean Sea, a species often misidentified as Laurencia dendroidea

Botanica Marina ◽

10.1515/bot-2020-0030 ◽

2020 ◽

Vol 63 (6) ◽

pp. 527-535

Author(s):

Donatella Serio ◽

Giovanni Furnari ◽

Yola Metti

Keyword(s):

Mediterranean Sea ◽

Molecular Analysis ◽

Molecular Data ◽

Integrative Approach ◽

Molecular Analyses ◽

The Mediterranean ◽

Laurencia Majuscula

AbstractIt was noted that Mediterranean specimens collected at different stations from around Sicily, Italy and referred to as Laurencia dendroidea (as Laurencia majuscula) were similar to the recently described species Laurenciella marilzae. Presented in this study are the results of an integrative approach using both morphology and molecular data (COI-5P + rbcL) to establish which taxon these specimens should be referred to. Molecular analyses show these specimens belong to Laurenciella, and strongly suggest they are within the species L. marilzae. Morphological examinations of these Mediterranean specimens were also detailed and found to support the conclusion that they belong to L. marilzae.

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