Fatty-acid synthesis in the echinoderms: Asterias rubens, Echinus esculentus and Holothuria forskali

1968 ◽  
Vol 48 (2) ◽  
pp. 521-533 ◽  
Author(s):  
W. V. Allen
Keyword(s):  
Fatty Acid ◽  
Acid Synthesis ◽  
Gas Liquid Chromatography ◽  
Asterias Rubens ◽  
Phospholipid Classes ◽  
Tissue Lipids ◽  
Specific Activities ◽  
Branched Chain ◽  
Layer Chromatography ◽  
Fatty Acid Compositions

The fatty-acid compositions of tissue lipids of Asterias rubens L., Echinus esculentus L. and Holothuria forskali Delia Chiaje were determined by means of gas-liquid chromatography. Considerable proportions of branched-chain, normal odd-numbered and eicosatetraenoic acids occur in the latter two animals. Tissues of all three animals were incubated with 1−14C-acetate. The relative specific activities of fatty-acid fractions isolated by thin-layer chromatography upon AgNO3-impregnated silica gel G fell in the order: saturates > monoenes — dienes > polyenes. The fatty-acid compositions of the alkoxydiglyceride, triglyceride and phospholipid classes of A. rubens hepatic caecal lipid were also determined.

FATTY ACID COMPOSITION OF NORMAL MINK TISSUES

1966 ◽  
Vol 44 (2) ◽  
pp. 179-185 ◽  
Author(s):  
B. L. Walker ◽  
V. F. Lishchenko
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Essential Fatty Acids ◽  
Methyl Esters ◽  
Dietary Factors ◽  
Gas Liquid Chromatography ◽  
Healthy Female ◽  
Kidney Liver ◽  
Layer Chromatography ◽  
Fatty Acid Compositions

Lipids, extracted from the adrenals, brain, erythrocytes, heart, kidney, liver, plasma, and spleen of normal healthy female mink, were transesterified with 1% sulphuric acid in methanol, and the resulting methyl esters were analyzed by gas–liquid chromatography after purification by thin-layer chromatography. All of the tissues examined contained higher concentrations of unsaturated than of saturated acids, the highest levels of unsaturated acids occurring in the lipids of heart, adrenals, and plasma, and of the essential fatty acids (ω6 series, with six carbon atoms after last double bond) in plasma, erythrocyte, and kidney lipids. The fatty acid compositions of mink tissues resemble those reported in the literature for the rat; detailed comparisons are not possible because of the known influence of dietary factors on tissue fatty acids.

scholarly journals Hepatic lipogenesis in young rats given proteins of different quality

1984 ◽  
Vol 52 (1) ◽  
pp. 131-137 ◽  
Author(s):  
G. R. Herzberg ◽  
Minda Rogerson
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Protein Quality ◽  
Fatty Acid Synthetase ◽  
Acid Synthesis ◽  
Hepatic Lipogenesis ◽  
Atp Citrate Lyase ◽  
Specific Activities ◽  
Glucose 6 Phosphate Dehydrogenase

1. The effect of feeding casein, lactalbumin, soya-bean protein, gluten or gelatin on hepatic lipogenesis and the levels of hepatic fatty acid synthetase (FAS), glucose-6-phosphate dehydrogenase (EC 1. 1. 1.49; G6PD), malic enzyme (EC 1. 1. 1.40; ME) ATP-citrate lyase (EC 4. 1. 3. 8; CL), acetyl CoA carboxylase (EC 6.4.1.2; ACCx) and glucokinase (EC 2. 7. 1. 2; GK) was examined in young growing rats.2. The total activities of ACCx, FAS, CL, GK, G6PD, GK, ME and fatty acid synthesis in vivo were positively correlated with protein quality.3. The specific activities of ACCx, FAS, CL, G6PD and fatty acid synthesis in vivo were positively correlated with protein quality.4. The specific activities of GK and ME were unrelated to protein quality.5. The results demonstrate a dissociation between ME and hepatic lipogenesis and suggest a role for the NADPH generated by ME which is not related to the needs of fatty acid synthesis.

scholarly journals Enzyme promiscuity drives branched-chain fatty acid synthesis in adipose tissues

2018 ◽  
Vol 14 (11) ◽  
pp. 1021-1031 ◽  
Author(s):  
Martina Wallace ◽  
Courtney R. Green ◽  
Lindsay S. Roberts ◽  
Yujung Michelle Lee ◽  
Justin L. McCarville ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Synthesis ◽  
Acid Synthesis ◽  
Chain Fatty Acid ◽  
Enzyme Promiscuity ◽  
Adipose Tissues ◽  
Branched Chain

scholarly journals Effect of Rearing System on the Straight and Branched Fatty Acids of Goat Milk and Meat of Suckling Kids

Foods ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 471
Author(s):  
Guillermo Ripoll ◽  
María Jesús Alcalde ◽  
Anastasio Argüello ◽  
María de Guía Córdoba ◽  
Begoña Panea
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Goat Milk ◽  
Lower Percentage ◽  
Dairy Goat ◽  
Branched Chain Fatty Acids ◽  
Branched Chain ◽  
Milk Replacers ◽  
Fatty Acid Compositions ◽  
Chain Fatty Acids

Goat meat is considered healthy because it has fewer calories and fat than meat from other traditional meat species. It is also rich in branched chain fatty acids that have health advantages when consumed. We studied the effects of maternal milk and milk replacers fed to suckling kids of four breeds on the straight and branched fatty acid compositions of their muscle. In addition, the proximal and fatty acid compositions of colostrum and milk were studied. Goat colostrum had more protein and fat and less lactose than milk. Goat milk is an important source of healthy fatty acids such as C18:1 c9 and C18:2 n–6. Suckling kid meat was also an important source of C18:1c9. Dairy goat breeds had higher percentages of trans monounsaturated fatty acids (MUFAs) and most of the C18:1 isomers but lower amounts of total MUFAs than meat breeds. However, these dairy kids had meat with a lower percentage of conjugated linoleic acid (CLA) than meat kids. The meat of kids fed natural milk had higher amounts of CLA and branched chain fatty acids (BCFAs) and lower amounts of n–6 fatty acids than kids fed milk replacers. Both milk and meat are a source of linoleic, α-linolenic, docosahexaenoic, eicosapentaenoic and arachidonic fatty acids, which are essential fatty acids and healthy long-chain fatty acids.

Influence of cytoplasms on the fatty acid composition of two maize inbred lines

1976 ◽  
Vol 86 (1) ◽  
pp. 151-154 ◽  
Author(s):  
P. Gregory ◽  
C. O. Grogan
Keyword(s):  
Fatty Acid Composition ◽  
Liquid Chromatography ◽  
Fatty Acid ◽  
Acid Composition ◽  
Inbred Lines ◽  
Gas Liquid Chromatography ◽  
Maize Seeds ◽  
Maize Inbred Lines ◽  
Oleic And Linoleic Acids ◽  
Fatty Acid Compositions

SUMMARYGas liquid chromatography was used to investigate the influence of several T, S and unclassified cytoplasms on the fatty acid composition of oil from A632 and CrS4HLA maize seeds. The fatty acid compositions of the A632 and CrS4HLA seeds differed markedly from each other with respect to oleic and linoleic acids but were stable within each seed line with respect to all sixteen of the cytoplasms tested.

Phosphatidylethanolamine in Liver Mitochondria and Endoplasmic Reticulum; Molecular Species Distribution and Turnover

1975 ◽  
Vol 53 (6) ◽  
pp. 698-705 ◽  
Author(s):  
J. G. Parkes ◽  
W. Thompson
Keyword(s):  
Endoplasmic Reticulum ◽  
Fatty Acid ◽  
Specific Activity ◽  
Liver Mitochondria ◽  
Relative Activity ◽  
Layer Chromatography ◽  
Kinetics Of ◽  
Fatty Acid Compositions

Phosphatidylethanolamine from mitochondria and microsomes of guinea pig liver was separated by thin-layer chromatography into eight different classes differing in degree of unsaturation. The fatty acid compositions and molar proportions of each class isolated from microsomes were very similar to the corresponding class in mitochondria. In both organelles about half of the total was dienoic species while tetraenes comprised approximately 20%. Stearic acid was the major saturated fatty acid and in each membrane a greater selectivity for stearate over palmitate was found in each sub-class of phosphatidylethanolamine, when compared with the corresponding class of phosphatidylcholine.Following the intraperitoneal injection of [2-3H]glycerol, the labelling of each molecular class of phosphatidylethanolamine showed very similar progressions in microsomes and mitochondria over a 3 h interval. In both organelles the highest relative specific activity was attained by penta-plus hexaenoic classes, while the large dienoic class had the lowest relative activity, which, however, increased with time. Analysis of the dienoic class of phosphatidylethanolamine from whole liver showed it to be constituted by a rapidly turning over palmitoyl–linoleoyl fraction and a slowly labelled stearoyl–linoleoyl fraction, a pattern also exhibited by dienoic phosphatidylcholines.The similarities in profile of molecular classes of phosphatidylethanolamine and in the kinetics of labelling in vivo point to a close metabolic relation between the lipids of both organelles, suggestive of a transfer of different molecular classes at comparable rates from the endoplasmic reticulum, the site of synthesis, to the mitochondria. This is consistent with numerous other studies in vitro that have demonstrated inter-organelle exchange of lipids.

Effect of substrate on fatty acid production in Nocardia asteroides

1970 ◽  
Vol 16 (4) ◽  
pp. 213-217 ◽  
Author(s):  
David Farshtchi ◽  
N. M. McClung
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fatty Acid Content ◽  
Fatty Acid Pattern ◽  
Cellular Fatty Acid ◽  
Nocardia Asteroides ◽  
Gas Liquid Chromatography ◽  
Fatty Acid Production ◽  
The Media ◽  
Branched Chain

Cellular fatty acids of the six Nocardia asteroides strains grown on glucose, glucose and amino acids, glycerol, and Dubos oleic albumin complex were determined by gas–liquid chromatography. Cells grown on each medium contained saturated, unsaturated, and branched-chain fatty acids. The fatty acids consisted of normal saturated C13, C14, C15, C16, C17, and C18; monoenoic C16 and C18; branched-chain C14, C15, and 10-methyl C18. Composition of the media affected cellular fatty acid content of N. asteroides strains qualitatively and quantitatively. Five of the six strains closely resembled each other, but one strain appeared to be different. The fatty acid pattern of Nocardia may be a useful criterion in differentiation of this genus from the closely related Mycobacterium and Streptomyces, which have a different fatty acid composition.

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