Total Antioxidant Capacity and Total Phenol Contents of Selected Commercial Fruit Juices in Turkey

2010 ◽  
Vol 13 (6) ◽  
pp. 1373-1379 ◽  
Author(s):  
Emine Nur Herken ◽  
Salih Guzel
2011 ◽  
Vol 5 (3) ◽  
pp. 405-407 ◽  
Author(s):  
Loredana Florina Leopold ◽  
Nicolae Leopold ◽  
Horst A. Diehl ◽  
Carmen Socaciu

Author(s):  
J. Olawuni Idowu ◽  
A. David Oyinade ◽  
S. Bamidele Funminiyi ◽  
E. Ogundepo Gbenga

Aim: To evaluated the antioxidant and radical scavenging ability of three different accessions (TGx-1835-10E, TGx-1987-62F and TGx 1951-3F) of soybean. Study Design: In vitro evaluation of antioxidant assays: Total phenol, Total flavonoid, Total antioxidant capacity, Ferric reducing antioxidant capacity, Cupric reducing antioxidant capacity, Ferrous Ion-chelating Ability, 2, 2-Diphenyl-2-Picryl-Hydrazyl (DPPH) and Nitric oxide (NO) radical scavenging activities. Place and Duration: Department of Biochemistry, Obafemi Awolowo University, Ile-Ife, Nigeria (August–December, 2016). Methodology: Hydroalcoholic crude extracts of TGx-1835-10E, TGx-1987-62F and TGx 1951-3F were obtained through soxhlet apparatus using 80% methanol and concentrated in a rotary evaporator at 4°C. The crude extract was then subjected to different  antioxidant assays (Total phenol, Total flavonoid, Total antioxidant capacity, Ferric reducing antioxidant capacity, Cupric reducing antioxidant capacity, Ferrous Ion-chelating Ability , DPPH and NO radical scavenging activities.) following standard procedures. Results: The results shows that TGx 1951-3F elicited the highest DPPH and NO radical scavenging activity with IC50 value of 2.61± 0.02 mg/ml and 2.58 ± 0.02 mg/ml, compared to TGx-1835-10E and TGx-1987-62F. Similarly, Ferrous Ion-chelating Ability (FIC) of TGx 1951-3F was higher with IC50 value of 1.38 ± 0.07 mg/ml, compared to TGx-1835-10E and TGx-1987-62F with an IC50 of 1.86 ± 0.16 and 2.07 ± 0.16 mg/ml. The reducing power of the three accessions expressed in terms of ascorbic acid equivalent tested using FRAP, TAC and CUPRAC assays showed that TGx 1951-3F has highest antioxidant activity follow by TGx-1835-10E and TGx-1987-62F. This same trend was also observed in antioxidant constituent present in the samples as TGx-1951-3F has higher phenolic and flavonoid content compared to TGx-1835-10E and TGx-1987-62F. Conclusion: The result of this present study revealed that Accession TGx 1951-3F elicit the highest antioxidant potential nevertheless, accessions TGx-1835-10E and TGx-1987-62F also contain significant amounts of flavonoids and phenolic compounds. Consequently, the plant seeds might be an important source of natural antioxidant, and helpful in prevention and management of various diseases associated with oxidative stress.


2015 ◽  
Vol 16 (2) ◽  
pp. 159-163
Author(s):  
Anjuman Ara Begum ◽  
Md Mosiul Haque ◽  
Mahfuzul Islam ◽  
Sukalyan Kumar Kundu

Flemingia macrophylla locally known as ‘Charchara’ in Bangladesh is a medicinal herb. Being a member of the Fabaceae family it claims a number of medicinal uses including hypoglycemic activity, neuroprotective effect and gynecological remedies. Methanolic extract of F. macrophylla was investigated to evaluate its antioxidant and general toxic properties in this study. Antioxidant potential was evaluated using total antioxidant capacity, total phenol contents, total flavonoid contents and DPPH (1,1-diphenyl-2-picrylhydrazyl) assays. Both leaf and stem extracts of F. macrophylla were found to possess significant amount of phenolics and flavonoids, expressed as gallic acid equivalents (GAE) and quercetin equivalent (QE), respectively. Interestingly total phenol content was equal for both leaf and stem extract and it was 43.8 mg GAE/g. Total flavonoid content was found to be 64.4 and 39.1 mg QE/g for leaf and stem extract, respectively. The total antioxidant capacity was expressed as ascorbic acid equivalents (AAE) and for leaf it was 5.067 mg/gm AAE and for stem 0.8167 mg/gm AAE. DPPH scavenging activity was determined by comparing with ascorbic acid. The IC50 values were 19.95, 125.89 and 707.94 ?g/ml for ascorbic acid, leaf and stem extract, respectively. The results of the present study on methanolic extracts of F. macrophylla revealed the presence of moderate antioxidant activity and extract of leaves produced better results than stem of the plant. In brine shrimp lethality bioassay, methanolic extract of F. macrophylla exhibited insignificant cytotoxicity. DOI: http://dx.doi.org/10.3329/bpj.v16i2.22298 Bangladesh Pharmaceutical Journal 16(2): 159-163, 2013


2011 ◽  
Vol 106 (2) ◽  
pp. 254-263 ◽  
Author(s):  
Meng Yang ◽  
Sang-Jin Chung ◽  
Chin Eun Chung ◽  
Dae-Ok Kim ◽  
Won O. Song ◽  
...  

Given the importance of dietary antioxidants in reducing the risks of chronic diseases, the present study aimed to estimate the intake of total antioxidant capacity (TAC) from diet and dietary supplements of US adults. We utilised the US Department of Agriculture flavonoid and proanthocyanidin databases, dietary supplement data and food consumption data of 4391 US adults aged 19+ years in the National Health and Nutrition Examination Survey 2001–2. In order to convert the intake data of individual antioxidant compounds to TAC values, the vitamin C equivalent (VCE) of forty-three antioxidant nutrients measured previously was also applied. Daily TAC averaged 503·3 mg VCE/d (approximately 75 % from diet and 25 % from supplements). The energy-adjusted daily TAC level from diet and supplements was higher in women (except for carotenoids), older adults, Caucasian (except for carotenoids), non-alcohol consumers (for vitamin E and proanthocyanidins), subjects with higher income (except for carotenoids) and higher exercise levels than their counterparts (P < 0·05). TAC was positively associated with daily consumption of fruits and fruit juices, vegetables and vegetable products, beverages, wines and teas (P < 0·001). Teas, dietary supplements, and fruits and fruit juices were the major sources of dietary TAC of the US population (28, 25 and 17 %, respectively), while the contribution of vegetables and vegetable products to TAC was minimal ( < 2 %). The present study indicates that antioxidant intake from various diet and supplements contributes to TAC status. TAC levels are different in sociodemographic subgroups of the US population. The relationship between TAC intake and risks of chronic disease warrants further investigation.


Molecules ◽  
2009 ◽  
Vol 14 (1) ◽  
pp. 480-493 ◽  
Author(s):  
Aurelia Magdalena Pisoschi ◽  
Mihaela Carmen Cheregi ◽  
Andrei Florin Danet

2019 ◽  
Vol 23 (2) ◽  
pp. 187-192
Author(s):  
Virun Vichaibun ◽  
Panan Kanchanaphu

Abstract Natural antioxidants from fruit juices have been interesting in terms of safety and low risk of side effects. Lemon juice was added to apple, grape, orange, pineapple and watermelon juices to obtain various concentrations. The blends were assayed for total phenolic content and total antioxidant capacity as well as superoxide dismutase (SOD)-like activity. The highest phenolic content was present in 50% (v/v) lemon juice in pineapple blend (425.1±5.1 μg/ml) in comparison to original juice. The total antioxidant capacity and SOD-like activity in the juice blends increased with increasing the proportion of lemon juice when compared with original juice. Our study indicated that lemon juice enhanced health benefits in terms of total phenolic content, total antioxidant capacity and SOD-like activity.


Author(s):  
Masoud Nasiri ◽  
Saja Ahmadizad ◽  
Mehdi Hedayati ◽  
Tayebe Zarekar ◽  
Mehdi Seydyousefi ◽  
...  

Abstract. Physical exercise increases free radicals production; antioxidant supplementation may improve the muscle fiber’s ability to scavenge ROS and protect muscles against exercise-induced oxidative damage. This study was designed to examine the effects of all-trans resveratrol supplementation as an antioxidant to mediate anti-oxidation and lipid per-oxidation responses to exercise in male Wistar rats. Sixty-four male Wistar rats were randomly divided into four equal number (n = 16) including training + supplement (TS), training (T), supplement (S) and control (C) group. The rats in TS and S groups received a dose of 10 mg/kg resveratrol per day via gavage. The training groups ran on a rodent treadmill 5 times per week at the speed of 10 m/min for 10 min; the speed gradually increased to 30 m/min for 60 minutes at the end of 12th week. The acute phase of exercise protocol included a speed of 25 m/min set to an inclination of 10° to the exhaustion point. Superoxide dismutase (SOD), glutathione peroxidase (GPx), catalase (CAT) activity, non-enzymatic antioxidants bilirubin, uric acid, lipid peroxidation levels (MDA) and the total antioxidant capacity (TAC) were measured after the exercise termination. The data were analyzed by using one-way ANOVA. The result showed that endurance training caused a significant increase in MDA level [4.5 ± 0.75 (C group) vs. 5.9 ± 0.41 nmol/l (T group)] whereas it decreased the total antioxidant capacity [8.5 ± 1.35 (C group) vs. 7.1 ± 0.55 mmol/l (T group)] (p = 0.001). In addition, GPx and CAT decreased but not significantly (p > 0.05). The training and t-resveratrol supplementation had no significant effect on the acute response of all variables except MDA [4.3 ± 1.4 (C group) vs. 4.0 ± 0.90 nmol/l (TS group)] (p = 0.001) and TAC [8.5 ± 0.90 (C group) vs. 6.6 ± 0.80 mmol/l (TS group)] (p = 0.004). It was concluded that resveratrol supplementation may prevent exercise-induced oxidative stress by preventing lipid peroxidation.


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