Immune response of guinea-pigs to chemically abbreviated prepatent Metastrongylus apri infection

Parasitology ◽  
1976 ◽  
Vol 72 (1) ◽  
pp. 13-18 ◽  
Author(s):  
V. Kumar ◽  
J. Mortelmans
Keyword(s):  
Guinea Pigs ◽  
Gamma Globulin ◽  
Immune Status ◽  
Clinical Manifestations ◽  
Control Group ◽  
Infection Dose ◽  
Infective Larvae ◽  
Worm Recovery ◽  
The Status ◽  
Experimental Group

The effect of chemical abbreviation of the primary infection dose (PID) of 160 infective larvae of Metastrongylus apri on the immune status of the guinea-pig host was studied. The criteria used for assessing the status of immunity consisted of clinical manifestations following administration of a challenge infection dose (CID) of 800 infective larvae of M. apri, the rate of worm recovery 15 days post-CID and the rate of mortality following administration of CID.Among the guinea-pigs of the main experimental group, where 15-day-old PID was abbreviated by two parenteral doses of levamisole*, a strong immunity to CID given 35 days post-PID was built-up. Against this, all the guinea-pigs of a control group, which did not receive PID, died between 16 and 22 days post-CID.The increase in serum gamma-globulin level of the guinea-pigs, where the PID was abbreviated chemically, suggested that the rise of this globulin fraction in the serum could be in some way related to the resistant state of guinea-pigs.

Immune response of guinea-pigs to chemically abbreviated prepatent Metastrongylus apri infection

Parasitology ◽  
1976 ◽  
Vol 72 (1) ◽  
pp. 13-18 ◽  
Author(s):  
V. Kumar ◽  
J. Mortelmans
Keyword(s):  
Guinea Pigs ◽  
Gamma Globulin ◽  
Immune Status ◽  
Clinical Manifestations ◽  
Control Group ◽  
Infection Dose ◽  
Infective Larvae ◽  
Worm Recovery ◽  
The Status ◽  
Experimental Group

SummaryThe effect of chemical abbreviation of the primary infection dose (PID) of 160 infective larvae of Metastrongylus apri on the immune status of the guinea-pig host was studied. The criteria used for assessing the status of immunity consisted of clinical manifestations following administration of a challenge infection dose (CID) of 800 infective larvae of M. apri, the rate of worm recovery 15 days post-CID and the rate of mortality following administration of CID.Among the guinea-pigs of the main experimental group, where 15-day-old PID was abbreviated by two parenteral doses of levamisole, a strong immunity to CID given 35 days post-PID was built-up. Against this, all the guinea-pigs of a control group, which did not receive PID, died between 16 and 22 days post-CID.The increase in serum gamma-globulin level of the guinea-pigs, where the PID was abbreviated chemically, suggested that the rise of this globulin fraction in the serum could be in some way related to the resistant state of guinea-pigs.

scholarly journals The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

2020 ◽  
Author(s):  
NURBEK SAIDILLAEVICH ALDAYROV ◽  
JARKINAY JUMABEKOVA ◽  
GULBUBU KURMANBEKOVA ◽  
NURJAMAL OMURZAKOVA ◽  
BERMET KIDIRALIEVA ◽  
...  
Keyword(s):  
Adult Male ◽  
Guinea Pigs ◽  
Structural Damage ◽  
Folk Medicine ◽  
Serum Testosterone ◽  
Serum Biochemistry ◽  
Raw Material ◽  
Control Group ◽  
Kyrgyz Republic ◽  
Experimental Group

Abstract BackgroundKuchala (Arum korolkowii Regel) is one of the medicinal plants often used in folk medicine in the Kyrgyz Republic. As a medicinal raw material, tubers tincture used in small doses to increase human sexual potency. However, there is no scientific evidence proving the medicinal effects of kuchala. For these reasons, we decided to study the effect of kuchala tuber tincture on the sexual potency of adult male guinea pigs.MethodsWe analyzed the effect of kuchala at age ±48-month-old, in 12 male guinea pigs. Prepared 10% tuber tincture of kuchala in 70% ethanol was administered perorally a once-daily dose of 150 µl for 30 days for the male guinea pigs. These study data were obtained by the ethological, hematological and serum biochemistry, gross anatomical, histological and statistical methods.ResultsThe hematological and serum biochemistry parameters were significantly different between control and experimental group. Neutrophils’ percentage in experimental group was significantly lower (dР < 0.001) than in control group. Lymphocyte counts on the contrary were significantly higher in experimental group (dР < 0.001). RBC counts, Hgb, Hct, MCH and MCHC were significantly higher in the experimental group (dР < 0.001; dР < 0.001; cР < 0.01; dР < 0.001; dР < 0.001 respectively) than control group. The color indicator and mean platelet volume contrary was higher (bР < 0.05) and significantly higher (dР < 0.001) in control animals than in the experimental. ALT and AST levels were lower in experimental than control group (both dР < 0,001). The testosterone concentration in serum was much higher (dР < 0,001) in the experimental group. Microscopically was found minor structural damage in the liver tissue that indicates a metabolic disorder. However, the testes showed an improvement in spermatogenesis in the experimental if compared with the control group.ConclusionThe 10% kuchala tuber tincture in 70% ethanol has a positive effect to improve the sexual potency of old guinea pigs by increasing the production of testosterone and increasing spermatogenesis.

scholarly journals The quality and safety of eggs obtained from laying hens after their experimental poisoning with sodium bromide

2020 ◽  
Vol 6 (1) ◽  
pp. 25-30
Author(s):  
О. Т. Kutsan ◽  
O. L. Orobchenko ◽  
Yu. M. Koreneva
Keyword(s):  
Laying Hens ◽  
Clinical Manifestations ◽  
Sodium Bromide ◽  
Control Group ◽  
Human Consumption ◽  
Egg Mass ◽  
Quality And Safety ◽  
Bromine Content ◽  
Poultry Eggs ◽  
Experimental Group

The purpose of this study was to determine the quality and safety of eggs obtained from laying hens after their experimental poisoning with sodium bromide. According to the principle of analogues, three experimental and one control group of laying hens (n = 15) were formed. The background bromine content of the compound feed was 2.0 mg/kg. An aqueous solution of sodium bromide was added daily to the feed of the chickens of the experimental groups for 28 days, followed by the observation of the birds for 14 days without its addition. Chickens of the 1st experimental group received bromine with feed at a dose of 10.0 mg/kg, 2nd — 50.0 mg/kg, 3rd — 250.0 mg/kg of feed. During the experiment, eggs were collected daily, their quality was determined according to the requirements of DSTU 5028:2008 ‘Hen’s Eggs for Human Consumption. Specifications’ and the rules for the veterinary and sanitary examination of poultry eggs. In addition, the bromine content was determined separately in egg white, yolk, and shell. Bromine content was determined by X-ray fluorescence analysis. Statistical processing of research results was conducted. Under the conditions of the chronic experiment, clinical manifestations of poisoning in chickens were not observed. No significant deviation from the control group was observed in productivity, egg mass, white to yolk mass ratios, and pH values of yolk and white. However, an uneven distribution of the eggs by category was established. Starting from the 2nd day of the experiment, bromine was excreted in laying hens with egg whites. The maximum value was observed on the 18th–28th days of the experiment: in the 1st experimental group a reliable excess 2.5 times of bromine content relative to the control was observed; in 2nd — 7.2 times, and in 3rd — 26.9 times. Thus, eggs from chickens of all groups conformed to DSTU 5028:2008 and the rules for the veterinary and sanitary examination of poultry eggs. However, the bromine content in the eggs of all experimental groups reliably exceeded the reference value for 28 days when sodium bromide was received with feed. Even 14 days after the experiment, the content of the element reliably exceeded the control value in the eggs of chickens from the 2nd and 3rd experimental groups, which may indicate the ability of bromine to cumulate

Influence of the new pre-starter feed on metabolism and immune status of piglets

2020 ◽  
pp. 42-51
Author(s):  
R. Milushev ◽  
D. Engovatov ◽  
V. Engovatov
Keyword(s):  
Immune Status ◽  
The Body ◽  
Favorable Effect ◽  
Control Group ◽  
Total Protein Content ◽  
Weaned Piglets ◽  
Suckling Piglets ◽  
Nitrogenous Substances ◽  
Experimental Group ◽  
Intestinal Microbiocenosis

A protein-vitamin-mineral concentrate (PVMC) containing plant protein has been developed for balancing compound feed for early weaned piglets. It uses a component from complete-fat fl axseeds, which contains polyunsaturated linolenic acid. The composition of the concentrate includes legumes, processed by special technology for the removal of anti-nutritional substances. Data on the effect of pre-starter compound feed containing the developed concentrate on the metabolism of nitrogenous substances in the body and some indicators of the neurohumoral link of the immunity of suckling pigs have been presented in the article. The animals had an intensive metabolism of nitrogenous substances. The total protein content in the blood serum was in the range of 65,53–65,73 g/l, and the ratio of albumins to globulins (coefficient A/G) was optimal – 1,55–1,67. The presence of a large amount of urea and glucose in the blood (3,05–3,72 and 6,60–5,80 mmol/l) has indicated that crossbred piglets had intensive synthetic processes associated with their growth. They contributed to the accumulation of M and G classes of immunoglobulins in the blood. The IgG content in the blood was 0,58–1,36 mg/ml, and IgM was 0,71–0,80 mg/ml. In terms of the amount of immunoglobulin G, the experimental group of piglets significantly exceeded the control group. Intestinal microbiocenosis in animals that have been received the new pre-starter compound feed was optimal. It has been dominated by positive microfloralactic acid and bifidum-bacteria, which inhibited the growth of pathogenic microflora, increasing the resistance of the piglets of the experimental group. Average daily gains in body weight were almost the same in both groups at the level of 253–254 g. Research results indicate that the new concentrate and pre-starter compound feed containing it had had the favorable effect on nitrogen metabolism and the immune status of the body of suckling piglets.

scholarly journals Psychological Identity and Family Upbringing

2021 ◽  
Author(s):  
Abdulrahman Alabdulwahed ◽  
Madher Attiat
Keyword(s):  
Control Group ◽  
First Year ◽  
Validity And Reliability ◽  
School Students ◽  
The Status ◽  
Quasi Experimental ◽  
Counselling Program ◽  
Experimental Group ◽  
Descriptive Method ◽  
Psychological Identity

This study aims to investigate the effectiveness of a counselling program to support psychological identity in the light of parental socialisation for a sample of first-year secondary school students. The sample (347) included students randomly selected from eight schools that represented all secondary schools in Dammam and 34 students who showed symptoms of identity disorder. These students were divided into two groups: one experimental group (17) and one control group (17). The research tools consisted of the Psychological Identity States Scale and the Parental Socialisation Styles Scale (their validity and reliability were verified) and a counselling program to improve cases of psychological identity disorder. A descriptive method was used here because it was suitable for identifying the students with psychological identity disorder. A quasi-experimental method was used to investigate the effectiveness of a counselling program in supporting psychological identity. The results showed that the most common cases of problems related to identity were: achieving identity, suspending identity, identity disorder and identity blocking. The most common patterns of family upbringing were: neglected, democratic, permissive, dispersed and authoritarian. There was a statistically significant effect of the indicative program in: improving the status of achieving identity, reducing two cases of identity lock and identity disorder. There was no significant effect of the indicative program in reducing the status of suspending identity.

scholarly journals The Effect of Kuchala (Arum Korolkowii Regel, 1873) Tuber Tincture to Increase of the Serum Testosterone in the Adult Male Guinea Pigs (Cavia Porcellus Linnaeus, 1758)

2021 ◽  
Author(s):  
Nurbek Aldayarov ◽  
Jarkinay Jumabekova ◽  
Gulbubu Kurmanbekova ◽  
Nurjamal Omurzakova ◽  
Bermet Kydyralieva ◽  
...  
Keyword(s):  
Adult Male ◽  
Guinea Pigs ◽  
Scientific Evidence ◽  
Folk Medicine ◽  
Serum Testosterone ◽  
Serum Biochemistry ◽  
Raw Material ◽  
Control Group ◽  
Kyrgyz Republic ◽  
Experimental Group

Abstract Background: Kuchala (Arum korolkowii Regel) is a medicinal plant often used in folk medicine in the Kyrgyz Republic. As a medicinal raw material, the tuber’s tincture is used in small doses to increase human sexual potency. However, there is no scientific evidence in support of the medicinal effects of kuchala. For these reasons, we decided to study the pharmacological effect of kuchala tuber tincture on the sexual potency of adult male guinea pigs. We analyzed the effect of kuchala at the age of ±48-months, in 12 male guinea pigs. A preparation of 10% tuber tincture of kuchala in 70% ethanol was administered perorally to the male guinea pigs in the form of a once-daily dose of 150 µl for 30 days. The study data were obtained by ethological, hematological and serum biochemistry, gross anatomical, histological and statistical methods.Results: The hematological and serum biochemistry parameters were significantly different between the control and the experimental group. The neutrophils’ percentage in the experimental group was significantly lower (dР < 0.001) than in the control group. On the other hand, the lymphocyte counts were significantly higher in the experimental group (dР < 0.001). The RBC counts, Hgb, Hct, MCH and MCHC were significantly higher in the experimental group (dР < 0.001; dР < 0.001; cР < 0.01; dР < 0.001; dР < 0.001 respectively) than in the control group. In contrast, the color indicator and the mean platelet volume were higher (bР < 0.05) and significantly higher (dР < 0.001) respectively in the control group than in the experimental group. The ALT and AST levels were lower in the experimental than in the control group (both dР < 0,001). The testosterone concentration in serum was much higher (dР < 0,001) in the experimental group. Microscopically, some structural damages were found in the liver of the experimental animals indicating a metabolic disorder. However, the testes showed an improvement in spermatogenesis in the experimental compared with the control group.Conclusions: The 10% kuchala tuber tincture in 70% ethanol has a positive effect in terms of improving the sexual potency of adult guinea pigs by increasing the production of testosterone and increasing spermatogenesis.

scholarly journals Effectiveness of using the drug “Bolus rozröd +” for prevention of obstetric polymorbid pathology of cows

2020 ◽  
Vol 23 (9) ◽  
pp. 45-56
Author(s):  
Olexandr Zubkov ◽  
Pavlo Skliarov
Keyword(s):  
Biochemical Parameters ◽  
Preventive Measures ◽  
Clinical Manifestations ◽  
Control Group ◽  
Total Cost ◽  
Blood Tests ◽  
Positive Effect ◽  
Experimental Group ◽  
Financial Losses

To prevent postpartum pathologies in cows, the study used the drug “Bolus rozröd +” – boluses containing ionized iodine (1.0%), selenium (0.15%), cobalt (0.50%) and copper (13.4%) and their long-term digestibility (within 6-8 months). The choice of the drug “Bolus rozröd +” as a means of prevention of postpartum pathologies is due to the fact that it contains trace elements that are deficient in the diet of the dairy complex “Ekaterinoslavsky”, the lack of which is a leading link in the etiopathogenesis of postpartum pathologies. A significant advantage is that the drug is relatively inexpensive (~ 10 $), and its components have a long digestibility (within 6-8 months), which can significantly reduce the frequency of injections (single, repeated ─ if necessary), and hence the total cost for preventive measures, treatment of sick animals and reduction of their stress during active milking, which will also reduce financial losses from unproduced milk. The experimental group (25) was given the drug “Bolus rozröd +”, heated to a temperature of 15-200C, orally, using an applicator (bolus) at the rate of 1 bolus per animal. The effectiveness of the drug “Bolus rozröd +” was determined according to the dynamics of biochemical blood tests and clinical evaluation of the postpartum period in cows. It was established that the method of prevention of postpartum pathologies in cows using the drug “Bolus rozrod +” is therapeutically effective, because it improves the biochemical parameters of the blood of animals, in particular, and reduces the incidence of postpartum pathology in general. Thus, the drug reduced the clinical manifestations of the following diseases: ketosis-metritis, placental delay and ketosis, and the number of healthy animals was 20% higher than in the control group, which proves the positive effect of the drug “Bolus rozröd +”.

scholarly journals THE EFFECTS OF CYCLOPHOSPHAMIDE AND ITS UROPROTECTIVE AGENTS, MESNA AND HYPERBARIC OXYGEN, ON URINARY BLADDER MOTILITY IN GUINEA PIGS

1999 ◽  
Vol 47 (4) ◽  
pp. 451-460
Author(s):  
Ö. ETLÝK ◽  
V. SAÐMANLIGÝL ◽  
Ý. PÝÞKÝN ◽  
A. Tomur
Keyword(s):  
Urinary Bladder ◽  
Hyperbaric Oxygen ◽  
Guinea Pigs ◽  
The Other ◽  
Control Group ◽  
Other Hand ◽  
Group 5 ◽  
Experimental Group ◽  
Cyclophosphamide Administration

The aim of this research was to observe the effects of cyclophosphamide and its uroprotective agents, mesna and hyperbaric oxygen (HBO), on the motility of urinary bladder muscle in guinea pigs. In the experimental groups, mesna and cyclophosphamide were intraperitoneally injected at a dose of 21.5 mg/kg and 68.1 mg/kg, respectively. For the combination of mesna and cyclophosphamide, one dose of mesna was injected 20 min before cyclophosphamide administration and three additional injections of mesna were repeated every three hours. A total of 8 HBO exposures were performed at 2.8 ATA for 90 min twice daily for another experimental group. In the HBO and cyclophosphamide combined group 5 HBO exposures were given prophylactically before cyclophosphamide. The combination of mesna, HBO and cyclophosphamide was administered by the same procedure. The contractions obtained in response to acetylcholine (ACh, 10–4M) in the control group were reduced using cyclophosphamide and HBO individually, but not by mesna. However, the contractions belonging to the various combinations of these three agents were not different from those seen in the control group. On the other hand, the combinations of cyclophosphamide, mesna and HBO showed higher responses to ACh than the groups in which cyclophosphamide and HBO were used individually, while the responses elicited by the cyclophosphamide and HBO combination were greater than those seen in the group treated with HBO only.

Effects of Repeated Bleedings on Serum Glycoprotein Concentrations in the Guinea Pig

1957 ◽  
Vol 190 (3) ◽  
pp. 529-532 ◽  
Author(s):  
Henry E. Weimer ◽  
Frances A. Quinn ◽  
Hisako Nishihara
Keyword(s):  
Blood Loss ◽  
Guinea Pig ◽  
Adult Male ◽  
Total Protein ◽  
Guinea Pigs ◽  
Control Group ◽  
Globulin Fraction ◽  
Serum Concentrations ◽  
Serum Glycoprotein ◽  
Experimental Group

Effects of repeated bleedings on the serum concentrations of total glycoprotein, seromucoid, albumin polysaccharide, globulin polysaccharide, total protein, albumin and globulin and on the hematocrit and hemoglobin values of blood were investigated in adult male guinea pigs. Significant increases occurred in the seromucoid fraction and in the carbohydrate and protein moieties of the globulin fraction. Significant decreases occurred in albumin polysaccharide, hemoglobin and hematocrit values. Statistical comparisons of the final values of the experimental group with those of a control group subjected to the same experimental procedures but without major blood loss were significant for total protein, albumin, hemoglobin and hematocrit. The conclusion was drawn that the alterations which occurred in the serum glycoprotein concentrations were not due primarily to blood loss.

scholarly journals Guinea pigs as experimental model to evaluate the resistance of the tissue expander capsule

2004 ◽  
Vol 19 (suppl 1) ◽  
pp. 96-103
Author(s):  
Fábio Xerfan Nahas ◽  
Luis O. Vasconez ◽  
Lydia Masako Ferreira
Keyword(s):  
Experimental Model ◽  
Guinea Pigs ◽  
Tissue Expander ◽  
Intraluminal Pressure ◽  
Control Group ◽  
Before And After ◽  
Total Capacity ◽  
Experimental Group ◽  
Hematoxylin Eosin

The purpose of this study is to report the use of guinea pigs as experimental model to study the resitance of the tissue expander capsule. Two groups were studied. In both groups a round 20 cc tissue expander, attached to a multiperforated catheter was inserted. The pocket housing was standardized. The skin over the expanded area was tattooed demarcating the area and arc, which were measured before and after each expansion. Every 4 days, a volume corresponding to 10% of the expander's total capacity was injected. Animals of the control group received 3 cc of saline through the catheter at the same time of expansion. Animals of the experimental group received 3 cc of the HBGF-1 diluted with saline. The intraluminal pressure of the expander was measured before and after the injection. After its total filling, the animals were sacrificed and 5 cc more were injected into each expander. The pressure was measured after each 1 cc injected. The capsule was examined histologically by immunofluorescence, trichrome and hematoxylin-eosin stains with the purpose of quantifying collagen and fibroblasts. The experimental model to study the resistance of the tissue expander's capsule showed to be feasible in guinea pigs.

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