Molecular characterization ofCryptosporidium parvumisolates from human cryptosporidiosis cases in Scotland

Parasitology ◽  
2014 ◽  
Vol 142 (2) ◽  
pp. 318-325 ◽  
Author(s):  
A. P. DESHPANDE ◽  
B. L. JONES ◽  
L. CONNELLY ◽  
K. G. POLLOCK ◽  
S. BROWNLIE ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cryptosporidium Parvum ◽  
Rural Areas ◽  
Molecular Diversity ◽  
Molecular Methods ◽  
Animal Disease ◽  
Human Feces ◽  
Microscopy Analysis ◽  
Subtyping Tool ◽  
Gp60 Subtyping

SUMMARYCryptosporidium parvum(C. parvum) is one of the most prevalent protozoan pathogens responsible for inducing human and animal disease worldwide. In this study, the glycoprotein-60 (gp60) subtyping tool was employed to assess the molecular diversity ofC. parvumfrom human feces throughout Scotland during potential outbreaks. Over a 24-month period, microscopy analysis revealed 1139 positive feces containingCryptosporidiumspecies with 256 identified by molecular methods specifically asC. parvum. Cryptosporidium parvumwas shown to be more prevalent in rural areas of Scotland and subtyping of 87 isolates demonstrated the predominant family as IIa, which occurred in 94% (n = 82) of isolates. The IIaA15G1R1 subtype was most common, being isolated from 47% (n = 41) of Scottish human cases. Non-IIa strains constituted a total of 5 isolates and included subtypes from the IIc, IId and IIg families. This information contributes significantly to existing knowledge and understanding ofC. parvumsubtypes in Scotland which is vital in assisting with the management of future local and national outbreaks.

Unusual cryptosporidiosis cases in Swedish patients: extended molecular characterization ofCryptosporidium viatorumandCryptosporidiumchipmunk genotype I

Parasitology ◽  
2013 ◽  
Vol 140 (14) ◽  
pp. 1735-1740 ◽  
Author(s):  
MARIANNE LEBBAD ◽  
JESSICA BESER ◽  
MONA INSULANDER ◽  
LILLEMOR KARLSSON ◽  
JENS G. MATTSSON ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cryptosporidium Parvum ◽  
Geographical Variation ◽  
Indian Subcontinent ◽  
Common Species ◽  
Diagnostic Methods ◽  
Molecular Diagnostic ◽  
Cryptosporidium Hominis ◽  
Genotype I ◽  
Protein Locus

SUMMARYMost human cases of cryptosporidiosis are caused byCryptosporidium parvumorCryptosporidium hominis, but the use of molecular diagnostic methods has revealed that several other less common species or genotypes can also be involved. Here, we describe two unusual causes of cryptosporidiosis, one being the recently described speciesCryptosporidium viatorumand the otherCryptosporidiumchipmunk genotype I. Two Swedish patients who were infected withC. viatorumhad travelled to Kenya and Guatemala, respectively, and two others had been infected withCryptosporidiumchipmunk genotype I in Sweden. None of these four patients were immunocompromised, and all four showed classical symptoms of cryptosporidiosis. We performed extensive molecular characterization, including analysis of four loci. The twoC. viatorumisolates were found to differ slightly at the 70-kDa heat shock protein locus, which may indicate a local geographical variation in this species that has previously been described exclusively on the Indian subcontinent.

Prevalence and molecular characterization of Hepatozoon canis in dogs from urban and rural areas in Southeast Brazil

2014 ◽  
Vol 97 (2) ◽  
pp. 325-328 ◽  
Author(s):  
R.L. de Miranda ◽  
L.H. O'Dwyer ◽  
J.R. de Castro ◽  
B. Metzger ◽  
A.S. Rubini ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Rural Areas ◽  
Hepatozoon Canis ◽  
Southeast Brazil ◽  
Urban And Rural Areas

scholarly journals Mycoplasma agalactiae: The Sole Cause of Classical Contagious Agalactia?

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1782
Author(s):  
Sergio Migliore ◽  
Roberto Puleio ◽  
Robin A. J. Nicholas ◽  
Guido R. Loria
Keyword(s):  
Diagnostic Tests ◽  
Clinical Signs ◽  
Small Ruminants ◽  
Molecular Methods ◽  
The Other ◽  
Animal Disease ◽  
Mycoplasma Agalactiae ◽  
Contagious Agalactia ◽  
Mycoplasma Capricolum ◽  
Mycoplasma Mycoides

Contagious agalactia (CA) is suspected when small ruminants show all or several of the following clinical signs: mastitis, arthritis, keratoconjunctivitis and occasionally abortion. It is confirmed following mycoplasma isolation or detection. The historical and major cause is Mycoplasma agalactiae which was first isolated from sheep in 1923. Over the last thirty years, three other mycoplasmas (Mycoplasma mycoides subsp. capri, Mycoplasma capricolum subsp. capricolum and Mycoplasma putrefaciens) have been added to the etiology of CA because they can occasionally cause clinically similar outcomes though nearly always in goats. However, only M. agalactiae is subject to animal disease regulations nationally and internationally. Consequently, it makes little sense to list mycoplasmas other than M. agalactiae as causes of the OIE-listed CA when they are not officially reported by the veterinary authorities and unlikely to be so in the future. Indeed, encouraging countries just to report M. agalactiae may bring about a better understanding of the importance of CA. In conclusion, we recommend that CA should only be diagnosed and confirmed when M. agalactiae is detected either by isolation or molecular methods, and that the other three mycoplasmas be removed from the OIE Manual of Diagnostic Tests and Vaccines in Terrestrial Animals and associated sources.

scholarly journals Ampelographic and molecular diversity among grapevine (Vitis spp.) cultivars

2009 ◽  
Vol 45 (No. 4) ◽  
pp. 160-168 ◽  
Author(s):  
A. Sabir ◽  
S. Tangolar ◽  
S. Buyukalaca ◽  
S. Kafkas
Keyword(s):  
Molecular Characterization ◽  
Molecular Diversity ◽  
Genetic Distances ◽  
Mean Values ◽  
Issr Analysis ◽  
Geographic Origins ◽  
The Mean ◽  
Vitis Spp

This study presents the ampelographic and molecular characterization of 44 grapevine cultivars. Ampelographic data were obtained during two vegetation periods using the latest version of the descriptors. Based on the mean values transformed by the method indicated in IBPGR publications, a dendrogram was constructed. ISSR analysis was also employed to characterize the genotypes at the DNA level. Twenty primers, selected on the basis of their discriminating potential, generated a total of 157 bands, of which 140 were polymorphic. The dendrograms constructed by the two approaches were largely similar in both the clustering position and divergence of varietal groups. The least distance was observed between Yuvarlak Cekirdeksiz and Superior Seedless. The clustering position of cultivars throughout the dendrograms was basically related to the genetic distances and main uses, as well as to geographic origins.

Molecular characterization of Danish Cryptosporidium parvum isolates

Parasitology ◽  
2002 ◽  
Vol 125 (04) ◽  
Author(s):  
H. L. ENEMARK ◽  
P. AHRENS ◽  
C. D. JUEL ◽  
E. PETERSEN ◽  
R. F. PETERSEN ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Cryptosporidium Parvum

Human and animal disease outbreaks in India due to mycotoxins other than aflatoxins

2009 ◽  
Vol 2 (1) ◽  
pp. 23-30 ◽  
Author(s):  
C. Raghavender ◽  
B. Reddy
Keyword(s):  
Health Risks ◽  
Rural Areas ◽  
South India ◽  
Animal Health ◽  
Disease Outbreaks ◽  
Western India ◽  
Animal Disease ◽  
The People ◽  
Contaminated Food ◽  
Significant Health

Mycotoxins are gaining increasing importance due to their deleterious effects on human and animal health. Chronic health risks are particularly prevalent in India where the diets of the people are highly prone to mycotoxins due to poor harvesting practices, improper storage and transport coupled with high temperature and moisture. This paper reviews disease outbreaks of mycotoxicoses other than aflatoxins in India due to ingestion of mycotoxincontaminated food. Ergotism is one of the earliest known outbreaks of mycotoxins reported in rural areas of western India associated with pearl millet grain. Trichothecenes have been involved in an acute human mycotoxicosis known as alimentary toxic aleukia in India during 1987 and were attributed to the consumption of mouldy wheat. Deoxynivalenol was implicated in an outbreak of emetic syndrome in Kashmir State. An outbreak of acute foodborne disease caused by fumonisin was reported in south India during 1995 affecting 1,424 people due to contaminated sorghum and maize. Rhizopus toxicosis was reported from Maharashtra State and caused the death of three people. These outbreaks continue to be a significant health problem of people in India, because their poor purchasing power compels them to consume contaminated food.

scholarly journals Shedding of Cryptosporidium in calves and dams: evidence of re-infection and shedding of different gp60 subtypes

Parasitology ◽  
2019 ◽  
Vol 146 (11) ◽  
pp. 1404-1413 ◽  
Author(s):  
Sarah Thomson ◽  
Elisabeth A. Innes ◽  
Nicholas N. Jonsson ◽  
Frank Katzer
Keyword(s):  
Cryptosporidium Parvum ◽  
Dairy Farm ◽  
First Year ◽  
Fragment Analysis ◽  
Adult Cattle ◽  
Fecal Samples ◽  
Common Causes ◽  
Second Year ◽  
Gp60 Subtypes ◽  
Gp60 Subtyping

AbstractOne of the most common causes of calf diarrhoea is the parasite Cryptosporidium parvum. Two longitudinal studies were carried out on a dairy farm Scotland to determine the prevalence of Cryptosporidium species and subtypes in a group of calves and to determine whether dams were a possible source of calfhood infection. Fecal samples were collected from 25 calves from birth to 12 months in the first year. In the second year, fecal samples were collected from pregnant cows (n = 29) and their calves (n = 30) from birth to 6 months. The samples were tested for Cryptosporidium and speciated. Cryptosporidium parvum-positive samples were subtyped by GP60 fragment analysis. All calves in both studies shed Cryptosporidium during the study period. Cryptosporidium parvum was the predominant species detected in calves ⩽6 weeks of age and at 6 months of age, C. bovis and C. ryanae were detected in calves older than 4 weeks of age but ⩽6 months of age. The prevalence of Cryptosporidium was higher in younger animals than in older animals. GP60 subtyping revealed two subtypes in calves on this farm (IIaA15G2R1 and IIaA19G2R1) that differed in frequency by age. Adult cattle also shed C. parvum, of four gp60 genotypes.

Two novel circo-like viruses detected in human feces: complete genome sequencing and electron microscopy analysis

2013 ◽  
Vol 178 (2) ◽  
pp. 364-373 ◽  
Author(s):  
Silvana Beres Castrignano ◽  
Teresa Keico Nagasse-Sugahara ◽  
Jonas José Kisielius ◽  
Marli Ueda-Ito ◽  
Paulo Eduardo Brandão ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Genome Sequencing ◽  
Complete Genome ◽  
Human Feces ◽  
Complete Genome Sequencing ◽  
Electron Microscopy Analysis ◽  
Microscopy Analysis

scholarly journals A multi-laboratory comparison of two molecular methods for the detection of toxigenic Clostridium difficile

2016 ◽  
Vol 10 (01) ◽  
pp. 62-67 ◽  
Author(s):  
Diane C Halstead ◽  
Joan Abid ◽  
Lynne Sloan ◽  
Diana Meza ◽  
Daphne Ramsey-Walker ◽  
...  
Keyword(s):  
Clostridium Difficile ◽  
Molecular Characterization ◽  
Regulatory Gene ◽  
Reference Method ◽  
Molecular Methods ◽  
Detection Methods ◽  
Molecular Testing ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Toxigenic Culture

Introduction: Diarrheal disease due to toxigenic Clostridium difficile (CD) accounts for an increased number of hospitalizations and deaths each year. Published guidelines recommend reflex testing of CD antigen-positive samples to molecular testing or testing samples directly by a molecular assay. This multicenter study was designed to compare the accuracy of two different molecular methods targeting different CD genes: Xpert C. difficile Epi RUO RT-PCR assay (XPCR) which targets toxin B (Cepheid, Sunnyvale, CA) and a laboratory-developed PCR (LDPCR) which targets mutations in the tcdC regulatory gene. Methodology: Two molecular methods for toxigenic CD detection, the Xpert C. difficile Epi RUO RT-PCR assay (XPCR) [Cepheid, Sunnyvale, CA] and a laboratory-developed PCR assay (LDPCR) were compared to a consensus gold standard (CGS) or toxigenic culture (TC) as the reference method. A subset of specimens was subjected to additional molecular characterization of toxigenic CD. Results: Both molecular methods were >90% sensitive for CD detection. Discordant results were noted when molecular test results were compared to non-molecular methods. Supplemental molecular characterization illustrated inherent difficulties in comparisons using different molecular methods for CD. Conclusion: Laboratories may consider using multiple CD detection methods or combinations of methods, including molecular detection for rapid and accurate diagnosis of CD, as driven by best practices for the respective healthcare environment. Laboratories must be aware of intrinsic differences when comparing performance characteristics of different molecular assays.

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