Effect of Ensiling and Rumen Digestion by Cattle on Weed Seed Viability

Weed Science ◽  
1991 ◽  
Vol 39 (1) ◽  
pp. 104-108 ◽  
Author(s):  
Robert E. Blackshaw ◽  
Lyle M. Rode

Studies were conducted to determine the effect of ensiling and/or rumen digestion by cattle on the germination and viability of several common weed species. Seed survival of grass species subjected to ensiling and/or rumen digestion tended to be less than that of broadleaf species. Downy brome, foxtail barley, and barnyardgrass were nonviable after either ensiling for 8 weeks or rumen digestion for 24 h. Some green foxtail (17%) and wild oats (0 to 88%) seeds survived digestion in the rumen but were killed by the ensiling process. Varying percentages of seeds of kochia, redroot pigweed, common lambsquarters, wild buckwheat, round-leaved mallow, and field pennycress remained viable after ensiling (3 to 30%), rumen digestion (15 to 98%), and ensiling plus rumen digestion (2 to 19%). A time course study of rumen digestion indicated that loss of seed viability often was not a gradual process. With some species, there was an initial lag phase while degradation of the protective seed coat likely occurred, followed by a rapid decline in embryo viability. The diet fed to livestock appeared to affect viability losses caused by rumen digestion. Estimates of seed survival with varying rates of passage through the rumen due to differing ratios of grain to forage in the diet are presented.

Weed Science ◽  
1999 ◽  
Vol 47 (5) ◽  
pp. 578-584 ◽  
Author(s):  
Robert G. Hartzler ◽  
Douglas D. Buhler ◽  
David E. Stoltenberg

Emergence characteristics, including initial time of emergence, magnitude of emergence, and mean time of emergence, ofAmaranthus rudisSauer,Setaria faberiHerrm.,Eriochloa villosa(Thunb.) Kunth, andAbutilon theophrastiMedik. were studied in central Iowa during the first 3 yr following burial of seed collected and buried in the fall of 1994 or 1995. Although the initial emergence date varied among years, the emergence sequence among species was consistent.Eriochloa villosaand A.theophrastiwere the first species to emerge, with initial emergence occurring between April 28 and May 10. Initial emergence dates for these species were the same, except for 1995 whenA. theophrastiemerged 4 d prior toE. villosa. Amaranthus rudiswas the last species to emerge, with initial emergence ranging from 5 to 25 d afterA. theophrasti.First-year emergence in 1995 was 8% forA. theophrasti, 7% for A.rudis, 41% forE. villosa, and 33% forS. faberi, based on the number of buried seed. Proportional emergence during the first year following burial in 1996 was similar to 1995 forA. theophrastiandS. faberi, but greater emergence was observed in 1996 forE. villosaandA. rudis.During the 3 yr of both studies, cumulative emergence of the two grass species (43 to 71%) was higher than for the broadleaf species (13 to 35%). A high percentage of the total annual emergence ofE. villosaoccurred within the first 2 wk of initial emergence, whereas a high percentage ofA. rudisemergence occurred late in its emergence period. Emergence characteristics of the four species were consistent among years and seed sources.


1998 ◽  
Vol 12 (2) ◽  
pp. 243-247 ◽  
Author(s):  
Edward E. Schweizer ◽  
Philip Westra ◽  
Donald W. Lybecker

Fifty irrigated cornfields in five eastern Colorado counties were sampled for their seedbanks and annual weed seedlings and mature populations between 1988 and 1992. Fourteen annual broadleaf species and seven annual grass species were identified in the 50 seedbanks sampled after the fields were tilled in the fall. Redroot pigweed and a mixture of green and yellow foxtail were the weed species encountered most, occurring in 90 and 54% of the fields, respectively. The single-plant populations of broadleaf and grass species in June and September were similar to those observed in the seedbanks. The number of weed species as seeds in the seedbank, June seedlings, and September plants per field ranged from zero to five grass species and zero to eight broadleaf species.


1993 ◽  
Vol 70 (02) ◽  
pp. 326-331 ◽  
Author(s):  
H R Lijnen ◽  
B Van Hoef ◽  
R A G Smith ◽  
D Collen

SummaryThe kinetic and fibrinolytic properties of a reversibly acylated stoichiometric complex between human plasmin and recombinant staphylokinase (plasmin-STAR complex) were evaluated. The acylation rate constant of plasmin-STAR by p-amidinophenyl-p’-anisate-HCI was 52 M-1 s-1 and its deacylation rate constant 1.2 × 10-4 s-1 (t½ of 95 min) which are respectively 50-fold and around 3-fold lower than for the plasmin-streptokinase complex. The acylated complex was stable as evidenced by binding to lysine-Sepharose. However, following an initial short lag phase, the acylated plasmin-STAR complex activated plasminogen at a similar rate as the unblocked complex, whereas the acylated plasmin-streptokinase complex did not activate plasminogen. These findings indicate that STAR, unlike streptokinase, dissociates from its acylated complex with plasmin in the presence of excess plasminogen. In agreement with this hypothesis, the time course of the lysis of a 125I-fibrin labeled plasma clot submerged in citrated human plasma, is similar for acylated plasmin-STAR, unblocked plasmin-STAR and free STAR (50% clot lysis in 2 h requires 12 nM of each agent). The plasma clearances of STAR-related antigen following bolus injection in hamsters were 1.0 to 1.5 ml/min for acylated plasmin-STAR, unblocked plasmin-STAR and free STAR, as a result of short initial half-lives of 2.0 to 2.5 min.The dissociation of the anisoylated plasmin-STAR complex and its consequent rapid clearance suggest that it has no apparent advantages as compared to free STAR for clinical thrombolysis.


Weed Science ◽  
2020 ◽  
pp. 1-29
Author(s):  
Lauren M. Schwartz-Lazaro ◽  
Lovreet S. Shergill ◽  
Jeffrey A. Evans ◽  
Muthukumar V. Bagavathiannan ◽  
Shawn C. Beam ◽  
...  

Abstract Potential effectiveness of harvest weed seed control (HWSC) systems depends upon seed shatter of the target weed species at crop maturity, enabling its collection and processing at crop harvest. However, seed retention likely is influenced by agroecological and environmental factors. In 2016 and 2017, we assessed seed shatter phenology in thirteen economically important broadleaf weed species in soybean [Glycine max (L.) Merr.] from crop physiological maturity to four weeks after physiological maturity at multiple sites spread across fourteen states in the southern, northern, and mid-Atlantic U.S. Greater proportions of seeds were retained by weeds in southern latitudes and shatter rate increased at northern latitudes. Amaranthus species seed shatter was low (0 to 2%), whereas shatter varied widely in common ragweed (Ambrosia artemisiifolia L.) (2 to 90%) over the weeks following soybean physiological maturity. Overall, the broadleaf species studied shattered less than ten percent of their seeds by soybean harvest. Our results suggest that some of the broadleaf species with greater seed retention rates in the weeks following soybean physiological maturity may be good candidates for HWSC.


2020 ◽  
pp. 1-22
Author(s):  
Michael J. Walsh ◽  
Annie E. Rayner ◽  
Annie Rutledge ◽  
John C. Broster

Abstract Chaff lining and chaff tramlining are harvest weed seed control (HWSC) systems that involve the concentration of weed seed containing chaff material into narrow (20 to 30 cm) rows between or on the harvester wheel tracks during harvest. These lines of chaff are left intact in the fields through subsequent cropping seasons in the assumption that the chaff environment is unfavourable for weed seed survival. The chaff row environment effect on weed seed survival was examined in field studies, while chaff response studies determined the influence of increasing amounts of chaff on weed seedling emergence. The objectives of these studies were to determine 1) the influence of chaff lines on the summer-autumn seed survival of selected weed species; and 2) the influence of chaff type and amount on rigid ryegrass seedling emergence. There was frequently no difference (P>0.05) in survival of seed of four weed species (rigid ryegrass, wild oat, annual sowthistle and turnip weed) when these seed were placed beneath or beside chaff lines. There was one instance where wild oat seed survival was increased (P<0.05) when seed were placed beneath compared to beside a chaff line. The pot studies determined that increasing amounts of chaff consistently resulted in decreasing numbers of rigid ryegrass seedlings emerging through chaff material. The suppression of emergence broadly followed a linear relationship where there was approximately a 2.0% reduction in emergence with every 1.0 t ha-1 increase in chaff material. This relationship was consistent across wheat, barley, canola and lupin chaff types, indicating that the physical presence of the chaff was more important than chaff type. These studies indicated that chaff lines may not affect the over summer-autumn survival of the contained weed seeds but the subsequent emergence of weed seedlings will be restricted by high amounts of chaff (>40 t ha-1).


1994 ◽  
Vol 8 (4) ◽  
pp. 840-848 ◽  
Author(s):  
Chester L. Foy ◽  
Susan B. Harrison ◽  
Harold L. Witt

Field experiments were conducted at two locations in Virginia to evaluate the following herbicides: alachlor, diphenamid, diuron, metolachlor, napropamide, norflurazon, oryzalin, oxyfluorfen, paraquat, pendimethalin, and simazine. One experiment involved newly-transplanted apple trees; the others, three in apple and one in peach trees, involved one-year-old trees. Treatments were applied in the spring (mid-April to early-May). Control of annual weed species was excellent with several treatments. A broader spectrum of weeds was controlled in several instances when the preemergence herbicides were used in combinations. Perennial species, particularly broadleaf species and johnsongrass, were released when annual species were suppressed by the herbicides. A rye cover crop in nontreated plots suppressed the growth of weeds. New shoot growth of newly-transplanted apple trees was increased with 3 of 20 herbicide treatments and scion circumference was increased with 11 of 20 herbicide treatments compared to the nontreated control. Growth of one-year-old apple trees was not affected. Scion circumference of one-year-old peach trees was increased with 25 of 33 herbicide treatments.


Weed Science ◽  
2013 ◽  
Vol 61 (1) ◽  
pp. 63-67 ◽  
Author(s):  
R. Bradley Lindenmayer ◽  
Scott J. Nissen ◽  
Philip P. Westra ◽  
Dale L. Shaner ◽  
Galen Brunk

Field bindweed is extremely susceptible to aminocyclopyrachlor compared to other weed species. Laboratory studies were conducted to determine if absorption, translocation, and metabolism of aminocyclopyrachlor in field bindweed differs from other, less susceptible species. Field bindweed plants were treated with 3.3 kBq14C-aminocyclopyrachlor by spotting a single leaf mid-way up the stem with 10 µl of herbicide solution. Plants were then harvested at set intervals over 192 h after treatment (HAT). Aminocyclopyrachlor absorption reached a maximum of 48.3% of the applied radioactivity by 48 HAT. A translocation pattern of herbicide movement from the treated leaf into other plant tissues emerged, revealing a nearly equal aminocyclopyrachlor distribution between the treated leaf, aboveground tissue, and belowground tissue of 13, 14, and 14% of the applied radioactivity by 192 HAT. Over the time-course, no soluble aminocyclopyrachlor metabolites were observed, but there was an increase in radioactivity recovered bound in the nonsoluble fraction. These results suggest that aminocyclopyrachlor has greater translocation to belowground plant tissue in field bindweed compared with results from other studies with other herbicides and other weed species, which could explain the increased level of control observed in the field. The lack of soluble metabolites also suggests that very little metabolism occurred over the 192 h time course.


1982 ◽  
Vol 60 (3) ◽  
pp. 389-397 ◽  
Author(s):  
Zbyszko F. Grzelczak ◽  
Mark H. Sattolo ◽  
Linda K. Hanley-Bowdoin ◽  
Theresa D. Kennedy ◽  
Byron G. Lane

The most prominent methionine-labeled protein made when cell-free systems are programmed with bulk mRNA from dry wheat embryos has been identified with what may be the most abundant protein in dry wheat embryos. The protein has been brought to purity and has a distinctive amino acid composition, Gly and Glx accounting for almost 40% of the total amino acids. Designated E because of its conspicuous association with early imbibition of dry wheat embryos, the protein and its mRNA are abundant during the "early" phase (0–1 h) of postimbibition development, and easily detected during "lag" phase (1–5 h), but they are almost totally degraded soon after entry into the "growth" phase of development, by about 10 h postimbibition.The most prominent methionine-labeled protein peculiar to the cell-free translational capacity of bulk mRNA from "growth" phase embryos is not detected as a product of in vivo synthesis. Its electrophoretic properties and its time course of emergence, after 5 h postimbibition development, suggest that this major product of cell-free synthesis may be an in vitro counterpart to a prominent methionine-labeled protein made only in vivo, by "growth" phase embryos. Designated G because of its conspicuous association with "growth" phase development, the cell-free product does not comigrate with any prominent dye-stained band in electrophoretic distributions of wheat proteins. The suspected cellular counterpart to G, also, does not comigrate with a prominent dye-stained wheat protein during electrophoresis, and although found in particulate as well as soluble fractions of wheat embryo homogenates it is not concentrated in either nuclei or mitochondria, as isolated.


2016 ◽  
Vol 14 (03) ◽  
pp. 1650007 ◽  
Author(s):  
Matthias Gerstgrasser ◽  
Sarah Nicholls ◽  
Michael Stout ◽  
Katherine Smart ◽  
Chris Powell ◽  
...  

Biolog phenotype microarrays (PMs) enable simultaneous, high throughput analysis of cell cultures in different environments. The output is high-density time-course data showing redox curves (approximating growth) for each experimental condition. The software provided with the Omnilog incubator/reader summarizes each time-course as a single datum, so most of the information is not used. However, the time courses can be extremely varied and often contain detailed qualitative (shape of curve) and quantitative (values of parameters) information. We present a novel, Bayesian approach to estimating parameters from Phenotype Microarray data, fitting growth models using Markov Chain Monte Carlo (MCMC) methods to enable high throughput estimation of important information, including length of lag phase, maximal “growth” rate and maximum output. We find that the Baranyi model for microbial growth is useful for fitting Biolog data. Moreover, we introduce a new growth model that allows for diauxic growth with a lag phase, which is particularly useful where Phenotype Microarrays have been applied to cells grown in complex mixtures of substrates, for example in industrial or biotechnological applications, such as worts in brewing. Our approach provides more useful information from Biolog data than existing, competing methods, and allows for valuable comparisons between data series and across different models.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5444
Author(s):  
Judith Sánchez-Blanco ◽  
Ernesto V. Vega-Peña ◽  
Francisco J. Espinosa-García

BackgroundDespite numerous tests of Darwin’s naturalization hypothesis (DNH) evidence for its support or rejection is still contradictory. We tested a DNH derived prediction stating that nonnative species (NNS) without native congeneric relatives (NCR) will spread to a greater number of localities than species with close relatives in the new range. This test controlled the effect of residence time (Rt) on the spread of NNS and used naturalized species beyond their lag phase to avoid the effect of stochastic events in the establishment and the lag phases that could obscure the NCR effects on NNS.MethodsWe compared the number of localities (spread) occupied by NNS with and without NCR using 13,977 herbarium records for 305 NNS of weeds. We regressed the number of localities occupied by NNSversus Rtto determine the effect of time on the spread of NNS. Then, we selected the species withRtgreater than the expected span of the lag phase, whose residuals were above and below the regression confidence limits; these NNS were classified as widespread (those occupying more localities than expected byRt) and limited-spread (those occupying fewer localities than expected). These sets were again subclassified into two groups: NNS with and without NCR at the genus level. The number of NNS with and without NCR was compared usingχ2tests and Spearman correlations between the residuals and the number of relatives. Then, we grouped the NNS using 34 biological attributes and five usages to identify the groups’ possible associations with spread and to test DNH. To identify species groups, we performed a nonmetric multidimensional scaling (NMDS) analysis and evaluated the influences of the number of relatives, localities, herbarium specimens,Rt, and residuals of regression. The Spearman correlation and the Mann–WhitneyUtest were used to determine if the DNH prediction was met. Additionally, we used the clustering objects on subsets of attributes (COSA) method to identify possible syndromes (sets of biological attributes and usages) associated to four groups of NNS useful to test DNH (those with and without NCR and those in more and fewer localities than expected byRt).ResultsResidence time explained 33% of the variation in localities occupied by nonnative trees and shrubs and 46% of the variation for herbs and subshrubs. The residuals of the regression for NNS were not associated with the number or presence of NCR. In each of the NMDS groups, the number of localities occupied by NNS with and without NCR did not significantly differ. The COSA analysis detected that only NNS with NCR in more and fewer localities than expected share biological attributes and usages, but they differ in their relative importance.DiscussionOur results suggest that DNH does not explain the spread of naturalized species in a highly heterogeneous country. Thus, the presence of NCR is not a useful characteristic in risk analyses for naturalized NNS.


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