Serratia marcescens Infections

The recognition of serratia as an opportunistic human pathogen can be dated from 1959, when the microorganism entered the family of Enterobacteriaceae, with features recognizable in the clinical laboratory and related to the Klebsiella/Enterobacter group. Since then, physicians have been challenged to establish the significance of isolation of serratia from a clinical specimen.

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Severe Acute Infection Due to Serratia marcescens Causing Respiratory Distress in An Immunocompetent Adult

Romanian Journal of Internal Medicine ◽

10.1515/rjim-2016-0013 ◽

2016 ◽

Vol 54 (2) ◽

pp. 134-136 ◽

Cited By ~ 2

Author(s):

Pablo Ruiz-Sada ◽

Mikel Escalante ◽

Eva Lizarralde

Keyword(s):

Risk Factors ◽

Respiratory Distress ◽

Serratia Marcescens ◽

Distress Syndrome ◽

Acute Infection ◽

Human Pathogen ◽

Immunocompetent Adult ◽

Uncommon Presentation ◽

Opportunistic Human Pathogen

AbstractThe role ofSerratia marcescenschanged from a harmless saprophytic microorganism to an important opportunistic human pathogen. It often causes nosocomial device-associated outbreaks and rarely serious invasive community acquired infections. We present a case of a community-acquiredSerratia marcescensbacteremia leading to Respiratory Distress Syndrome in a previously healthy 51-year-old man without identifiable risk factors. Full recovery was achieved with solely medical treatment and observation in ICU during three days. To our knowledge it is an extremely uncommon presentation and just few cases have been previously reported in the literature.

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A High-Throughput Short Sequence Typing Scheme for Serratia marcescens Pure Culture and Environmental DNA

Applied and Environmental Microbiology ◽

10.1128/aem.01399-21 ◽

2021 ◽

Author(s):

Thibault Bourdin ◽

Alizée Monnier ◽

Marie-Ève Benoit ◽

Emilie Bédard ◽

Michèle Prévost ◽

...

Keyword(s):

Serratia Marcescens ◽

High Throughput ◽

Neonatal Intensive Care ◽

Environmental Dna ◽

Short Sequence ◽

Human Pathogen ◽

Opportunistic Pathogens ◽

Environmental Features ◽

Opportunistic Human Pathogen ◽

Sink Location

Molecular typing methods are used to characterize the relatedness between bacterial isolates involved in infections. These approaches rely mostly on discrete loci or whole genome sequences (WGS) analyses of pure cultures. On the other hand, their application to environmental DNA profiling to evaluate epidemiological relatedness amongst patients and environments has received less attention. We developed a specific, high-throughput short sequence typing (HiSST) method for the opportunistic human pathogen Serratia marcescens . Genes displaying the highest polymorphism were retrieved from the core genome of 60 S. marcescens strains. Bioinformatics analyses showed that use of only three loci (within bssA , gabR and dhaM ) distinguished strains with a high level of efficiency. This HiSST scheme was applied to an epidemiological survey of S. marcescens in a neonatal intensive care unit (NICU). In a first case study, a strain responsible for an outbreak in the NICU was found in a sink drain of this unit, by using HiSST scheme and confirmed by WGS. The HiSST scheme was also applied to environmental DNA extracted from sink-environment samples. Diversity of S. marcescens was modest, with 11, 6 and 4 different sequence types (ST) of gabR , bssA and dhaM loci amongst 19 sink drains, respectively. Epidemiological relationships amongst sinks were inferred on the basis of pairwise comparisons of ST profiles. Further research aimed at relating ST distribution patterns to environmental features encompassing sink location, utilization and microbial diversity is needed to improve the surveillance and management of opportunistic pathogens. Importance Serratia marcescens is an important opportunistic human pathogen, multidrug resistant and often involved in outbreaks of nosocomial infections in neonatal intensive care unit. Here, we propose a quick and user-friendly method to select the best typing scheme for nosocomial outbreaks in relating environmental and clinical sources. This method, named high-throughput short sequence typing (HiSST), allows to distinguish strains and to explore the diversity profile of non-culturable S. marcescens . The application of HiSST profile analysis for environmental DNA offers new possibilities to track opportunistic pathogens, identify their origin and relate their distribution pattern with environmental features encompassing sink location, utilization and microbial diversity. Adaptation of the method to other opportunistic pathogens is expected to improve knowledge regarding their ecology, which of significant interest for epidemiological risk assessment and elaborate outbreak mitigation strategies.

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Serratia no longer an opportunistic uncommon pathogen – case series & review of literature

Infectious Disorders - Drug Targets ◽

10.2174/1871526521666210222125215 ◽

2021 ◽

Vol 21 ◽

Author(s):

Varsha Gupta ◽

Shiwani Sharma ◽

Kritika Pal ◽

Poonam Goyal ◽

Deepak Agarwal ◽

...

Keyword(s):

Serratia Marcescens ◽

Case Series ◽

Opportunistic Pathogen ◽

Morphological Characters ◽

Enteric Bacterium ◽

Human Pathogen ◽

Review Of Literature ◽

Medical College ◽

Serious Infections ◽

The Family

Background: Serratia spp. is a common enteric bacterium generally thought not to be pathogenic in the gastrointestinal tract. Serratia marcescens is a member of the genus Serratia, which is a part of the family Enterobacteriales..Of all Serratia species, S. marcescens is the most common clinical isolate and the most important human pathogen. Objective: We are discussing here four cases of Serratia marcescens which we reported in our laboratory in the Department of Microbiology Government Medical College and Hospital Chandigarh during six months of duration. Method: All the samples were processed and identified as per standard microbiological techniques.The isolates of Serratia marcescens were identified, depending upon their biochemical and morphological characters and further confirmed by MALDI-TOF-MS ,PGIMER Chandigarh. Result: In one of the four cases there was polymicrobial infection and one patient was diabetic and rest three patients were immunocompetent. The importance of detection and reporting of Serratia marcescens is related to the concern regarding its increase spread in hospital settings as nosocomial infection . Conclusion: We need to identify and isolate this pathogen ,not thinking of only contaminant and opportunistic pathogen but as a pathogen which can lead to serious infections in hospital settings .

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A generalized transducing phage (ϕIF3) for the genomically sequenced Serratia marcescens strain Db11: a tool for functional genomics of an opportunistic human pathogen

Microbiology ◽

10.1099/mic.0.28712-0 ◽

2006 ◽

Vol 152 (6) ◽

pp. 1701-1708 ◽

Cited By ~ 45

Author(s):

Nicola K. Petty ◽

Ian J. Foulds ◽

Elizabeth Pradel ◽

Jonathan J. Ewbank ◽

George P. C. Salmond

Keyword(s):

Serratia Marcescens ◽

Reference Strain ◽

Burst Size ◽

Genomic Analysis ◽

Restriction Enzymes ◽

Functional Genomic Analysis ◽

The Family ◽

Phage Dna ◽

Opportunistic Human Pathogen ◽

Generalized Transduction

A bacteriophage (ϕIF3) capable of mediating generalized transduction in Serratia marcescens strain Db11 has been isolated and characterized. The genome of this Serratia strain has recently been sequenced and is likely to become the reference strain for S. marcescens researchers. ϕIF3 is most likely a virulent phage, which can transduce markers at frequencies of 10−6 transductants per p.f.u. It has a lipopolysaccharide receptor and was determined to have a latent period of 50 min and a burst size of approximately 100 phages. The phage DNA was resistant to digestion with restriction enzymes. Electron microscopy showed ϕIF3 to be a member of the family Myoviridae. This is the first report of a generalized transducing phage able to infect Db11 and this phage will be a valuable tool for functional genomic analysis of the pathogen host.

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Complete Genome Sequence of Serratia marcescens Podophage Parlo

Microbiology Resource Announcements ◽

10.1128/mra.00569-19 ◽

2019 ◽

Vol 8 (27) ◽

Cited By ~ 3

Author(s):

Ryan Bockoven ◽

Jamie Gutierrez ◽

Heather Newkirk ◽

Mei Liu ◽

Jesse Cahill ◽

...

Keyword(s):

Serratia Marcescens ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Resistance Mechanisms ◽

Full Genome Sequence ◽

Multiple Resistance ◽

Full Genome ◽

Human Pathogen ◽

Content Type ◽

Opportunistic Human Pathogen

ABSTRACT Serratia marcescens is an opportunistic human pathogen with multiple resistance mechanisms that infects hospitalized patients. Here, we report the full genome sequence of S. marcescens podophage Parlo. Parlo is most similar to Erwinia phage PEp14 and encodes a 3,764-residue protein assumed to be a homolog of DarB, an antirestriction protein.

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Complete Genome Sequence of Serratia marcescens Siphophage Serbin

Microbiology Resource Announcements ◽

10.1128/mra.00422-19 ◽

2019 ◽

Vol 8 (19) ◽

Cited By ~ 3

Author(s):

Eric A. Williams ◽

Helena Hopson ◽

Andrea Rodriguez ◽

Rohit Kongari ◽

Rachele Bonasera ◽

...

Keyword(s):

Urinary Tract ◽

Serratia Marcescens ◽

Genome Sequence ◽

Complete Genome Sequence ◽

Complete Genome ◽

Human Pathogen ◽

Content Type ◽

Opportunistic Human Pathogen ◽

Tract Infections ◽

Hospital Acquired

Serratia marcescens is an opportunistic human pathogen that is known to cause hospital-acquired respiratory and urinary tract infections. Here, we announce the complete genome sequence and the features of S. marcescens phage Serbin.

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Characterisation of a tripartite α-pore forming toxin from Serratia marcescens

Scientific Reports ◽

10.1038/s41598-021-85726-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Alicia M. Churchill-Angus ◽

Thomas H. B. Schofield ◽

Thomas R. Marlow ◽

Svetlana E. Sedelnikova ◽

Jason S. Wilson ◽

...

Keyword(s):

Sequence Analysis ◽

Serratia Marcescens ◽

Conformational Change ◽

Pore Formation ◽

Gram Negative Bacteria ◽

Human Pathogen ◽

Gram Negative ◽

The Family

AbstractTripartite members of the ClyA family of α-PFTs have recently been identified in a number of pathogenic Gram-negative bacteria, including the human pathogen Serratia marcescens. Structures of a Gram-negative A component and a tripartite α-PFT complete pore are unknown and a mechanism for pore formation is still uncertain. Here we characterise the tripartite SmhABC toxin from S. marcescens and propose a mechanism of pore assembly. We present the structure of soluble SmhA, as well as the soluble and pore forms of SmhB. We show that the β-tongue soluble structure is well conserved in the family and propose two conserved latches between the head and tail domains that are broken on the soluble to pore conformational change. Using the structures of individual components, sequence analysis and docking predictions we illustrate how the A, B and C protomers would assemble on the membrane to produce a complete tripartite α-PFT pore.

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The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis

Acta Crystallographica Section F Structural Biology Communications ◽

10.1107/s2053230x20013862 ◽

2020 ◽

Vol 76 (12) ◽

pp. 577-582

Author(s):

Alicia M. Churchill-Angus ◽

Svetlana E. Sedelnikova ◽

Thomas H. B. Schofield ◽

Patrick J. Baker

Keyword(s):

Serratia Marcescens ◽

Electron Density ◽

Crystallographic Analysis ◽

Gram Negative Bacteria ◽

Human Pathogen ◽

Gram Negative ◽

Anomalous Data ◽

Density Map ◽

Opportunistic Human Pathogen ◽

Electron Density Map

Tripartite α-pore-forming toxins are constructed of three proteins (A, B and C) and are found in many bacterial pathogens. While structures of the B and C components from Gram-negative bacteria have been described, the structure of the A component of a Gram-negative α-pore-forming toxin has so far proved elusive. SmhA, the A component from the opportunistic human pathogen Serratia marcescens, has been cloned, overexpressed and purified. Crystals were grown of selenomethionine-derivatized protein and anomalous data were collected. Phases were calculated and an initial electron-density map was produced.

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