Ultrastructure of the Surface Epithelium of the Polycystic Ovary in Constant Light Rats

1982 ◽  
Vol 40 ◽  
pp. 240-241
Author(s):  
K. B. Singh ◽  
F. K. Khosho
Keyword(s):  
Electron Microscopy ◽  
Polycystic Ovary ◽  
Continuous Light ◽  
Female Rats ◽  
Constant Light ◽  
Surface Epithelium ◽  
Transmission Electron ◽  
Persistent Estrus ◽  
Microscopic Studies ◽  
Pco Syndrome

It is now well established that exposure of cyclic female rats to continuous light induces persistent estrus (PE), a syndrome which in many respects resembles the human polycystic ovary (PCO) syndrome. As in the human PCO, light microscopic studies of cystic ovaries of the constant light rat show numerous atretic follicles, and the surface epithelium grossly appears smooth and white. In the present study, we have utilized scanning (SEM) and transmission electron microscopy (TEM) to study changes on the surface epithelium of PCO in the constant light rat model.

Scanning Electron Microscopy of the Polycystic Ovary in Constant Light Rats

1981 ◽  
Vol 39 ◽  
pp. 538-539
Author(s):  
K. B. Singh ◽  
F. K. Khosho
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Polycystic Ovary ◽  
Three Dimensional ◽  
Continuous Light ◽  
Female Rats ◽  
Constant Light ◽  
Sprague Dawley ◽  
Polycystic Ovaries ◽  
Scanning Electron

Exposure of female rats to continuous light eventually induces persistent estrus (PE), a syndrome which in many respects resembles the human polycystic ovary syndrome. Light microscopic studies on the polycystic ovaries of constant light PE rats have been previously reported. In the present study, we have utilized scanning electron microscopy (SEM) to provide information regarding three-dimensional changes occurring on the surface of the polycystic ovary.Following exposure to continuous light for 50 days, PE was induced in a group of Sprague-Dawley female rats according to the procedure described elsewhere (2). Polycystic ovaries from constant light PE rats and ovaries from controls were fixed in 2.5% glutaraldehyde in 0.1M Na cacodylate buffer, pH 7.3 at room temperature for 24 hours. They were then cut into two equal halves along their long axis and fixed for an additional 3-4 hours. The specimens were processed for SEM studies by a modified OTOTO technique, dehydrated and critical point dried with CO2.

Vaginal microflora in a rat model of polycystic ovarian disease

1983 ◽  
Vol 41 ◽  
pp. 560-561
Author(s):  
K.B. Singh ◽  
Ram P. Tewari ◽  
F. K. Khosho
Keyword(s):  
Reproductive Tract ◽  
Continuous Light ◽  
Hormonal Control ◽  
Female Rats ◽  
Surface Epithelium ◽  
Polycystic Ovaries ◽  
Vaginal Microflora ◽  
Sterile Normal Saline ◽  
Persistent Estrus

Exposure of female rats to continuous light for prolonged periods induces persistent estrus (PE), an anovulatory disorder. Instead of cyclic fluctuations, the vaginal smears from PE rats show only cornified cells as the hormonal control of the estrous cycle becomes inappropriate, and ovaries become cystic when ovulation ceases. The reproductive tract, a key target organ in PE, shows numerous manifestations of chronic estrogenic influence.In this paper, we report our findings on bacterial colonizations and ultrastructural examinations of the vaginal surface epithelium of PE rats with polycystic ovaries (PCO). Quantitation of bacteria in the vaginal lavage was done with a standard volume of sterile normal saline and studied in five different hormonal states: diestrus day 1 (D-1), diestrus day 2 (D2), proestrus (Pro E), estrus (E), and persistent estrus (PE). Scanning electron microscopy (SEM) was used to examine the vaginal surface epithelium from PE rats with PCO and compared to the controls. In all rats, cardiovascular perfusion was performed for in vivo fixation of the organs using 2.5% glutaraldehyde in 0.1 M Na Cacodylate buffer (pH 7.3) for 30 minutes.

Surface Changes in Vaginal Epithelial Cells During Persistent Estrous Rats

1985 ◽  
Vol 43 ◽  
pp. 656-657
Author(s):  
Khosho Francis K. ◽  
Kaufmann Robert C. ◽  
Amankwah Kofi S.
Keyword(s):  
Polycystic Ovary ◽  
Female Rats ◽  
Constant Light ◽  
Major Influence ◽  
Surface Epithelium ◽  
Female Rat ◽  
Vaginal Epithelial Cells ◽  
Cacodylate Buffer ◽  
And Control ◽  
Scanning Electron

Light exerts a major influence on the reproductive functions of the female rat, and the presence of constant light results in an aberrant vaginal cycle, leading to infertility associated with polycystic ovary (PCO) syndrome. In the present communication, we have utilized scanning electron microscopy (SEM) to study changes on the surface epithelium of persistent estrous (PE) rats with PCO.Sprague-Dawely female rats were exposed to constant light for 50-150 days according to protocol that has been previously described. Rats in normal estrous, as determined by vaginal smears, were used as controls. Nembutal- anesthesized rats were perfused through the aorta with 2.5% gluteraldehyde in 0.1M sodium cacodylate buffer (pH 7.3). The mucosa of the upper third of the vagina from PE and control rats were microdissected, processed for SEM studies by a modified 0T0T0 technique, dehydrated, and critical point dried with C02- The specimens were examined in a Hitachi scanning electron microscope Model S-500.

INTERACTIONS OF THE PINEAL AND EXPOSURE TO CONTINUOUS LIGHT ON ORGAN WEIGHTS OF FEMALE RATS

1961 ◽  
Vol 36 (4) ◽  
pp. 617-624 ◽  
Author(s):  
Richard Jay Wurtman ◽  
Willard Roth ◽  
Mark D. Altschule ◽  
Judith J. Wurtman
Keyword(s):  
Light Exposure ◽  
Continuous Light ◽  
Female Rats ◽  
Constant Light ◽  
Ovarian Weight ◽  
Organ Weights ◽  
Bovine Pineal Extracts ◽  
Pineal Extracts ◽  
Effect Of Light

ABSTRACT Either exposure to constant light for 80 days or pinealectomy produced similar changes in the weights of the ovaries and adrenals of female rats. These were not additive when both procedures were employed. Pinealectomy did not share with light-exposure the capacity to induce uterine hypertrophy. Rats exposed to constant light for 56 days had lighter pineals than animals kept in darkness; this decrease was not affected by administration of bovine pineal extracts. The increase in ovarian weight produced in rats by exposure to light for 56 days was prevented by bovine pineal extracts, but these extracts were without effect on the uterine hypertrophy produced under the same conditions. These data suggest that the effect of light upon the weight of the ovary is mediated via the pineal.

Re-evaluation of merogony of a Cystoisospora ohioensis-like coccidian and its distinction from gametogony in the intestine of a naturally infected dog

Parasitology ◽  
2019 ◽  
Vol 146 (6) ◽  
pp. 740-745
Author(s):  
J. P. Dubey
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Light Microscopy ◽  
Developmental Stages ◽  
Surface Epithelium ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Sexual Stages ◽  
Endogenous Stages ◽  
Study Development

AbstractFour species of Cystoisospora, C. canis, C. ohioensis, C. neorivolta and C. burrowsi are described from feces of dogs. Of these, the oocysts of C. canis are the largest and easily distinguished from the remaining three species. Oocysts of C. ohioensis, C. neorivolta and C. burrowsi are difficult to distinguish because of overlap in their sizes. However, based on endogenous developmental stages, C. ohioensis is distinct from C. neorivolta and C. burrowsi because its endogenous stages are confined to surface epithelium of intestine whereas endogenous stages of C. neorivolta and C. burrowsi are predominantly in the lamina propria. There are uncertainties regarding the endogenous stages of C. neorivolta and C. burrowsi and there is no way now to determine whether C. burrowsi and C. neorivolta are different parasites; therefore, these are referred as C. ohioensis-like organisms. Additionally, mode of division of asexual stages of coccidia of dogs is largely unknown and ultrastructural studies are lacking. In the present study, development of asexual and sexual stages of a C. ohioensis-like organism in a naturally infected dog is described by light microscopy and by transmission electron microscopy. Merozoites divided by endodyogeny/merogony. Meronts were crescent/merozoite-shaped and contained a maximum of eight nuclei. A distinctive feature of merozoites was the presence of many PAS-positive amylopectin granules that were absent or rare in immature microgamonts making it possible to distinguish them.

High-Resolution SEM of Colloidal Gold Labels

1988 ◽  
Vol 46 ◽  
pp. 214-217
Author(s):  
Ralph M. Albrecht ◽  
Scott R. Simmons ◽  
James R. Prudent ◽  
Chris M. Erickson
Keyword(s):  
Electron Microscopy ◽  
Colloidal Gold ◽  
Spherical Shape ◽  
Biologically Active ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Color Density ◽  
Microscopic Studies ◽  
Backscattered Electron ◽  
Electron Imaging

Colloidal gold, conjugated to a number of biologically active molecules, including ligand and antibody, provides a useful label for light microscopy and electron microscopy. This stems, in part, from its color, density, and regular spherical shape although the ability to make the particles in a number of defined sizes, the ease of conjugation to biological material, and the retention of activity of bound molecules are also important factors.Although nearly all sizes of colloidal gold particles, from 2.0 nm on up, can be identified in transmission or high voltage transmission electron microscopy, it has generally been the larger sized particles, 15 nm and up, that have proved useful for scanning electron microscopic studies. This is due principally to the resolution limits of conventional SEMs and the need to employ backscattered electron imaging, BEI, to unambiguously define the gold labels.

Ultrastructural and Scanning Electron Microscopic Studies of Basal Cell Carcinoma

1980 ◽  
Vol 38 ◽  
pp. 728-729
Author(s):  
A. Lupulescu
Keyword(s):  
Electron Microscopy ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Basal Cells ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Studies ◽  
And Migration ◽  
Scanning Electron

Previously it has been shown that long-term topical application of 3-methylcholanthrene (MCA) on the rat skin induced basal cell carcinoma. These tumors are very similar to that occurring in humans and they were studied only by light microscopy.1 Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) can provide more characteristic details for the neoplastic transformation of basal cells, their cytoarchitecture and migration.

Electron Microscopy Studies of Polymer Blends

1980 ◽  
Vol 38 ◽  
pp. 248-249
Author(s):  
S.Y. Hobbs ◽  
V.H. Watkins ◽  
R.P. Kambour ◽  
R.C. Bopp
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Phase Separation ◽  
Phase System ◽  
Segregation Behavior ◽  
Phenylene Oxide ◽  
Transmission Electron ◽  
Microscopic Studies ◽  
Phase Separation Behavior ◽  
Separation Behavior

Scanning and transmission electron microscopy have been used to investigate the phase separation behavior of blends of brominated poly (2,6-dimethy1 -1 ,4-phenylene oxide) known trivially as poly (xylenyl ether) (PXE) and polystyrene. DSC scans show that when more than 85% of the PXE rings are brominated, the blends exhibit two glass temperatures centered at 100°C and 290°C and the polymers are immiscible.1 At lower bromine concentrations, a single composition dependent Tg characteristic of a one phase system is observed. Microscopic studies, however, indicate that separate phases which can not be detected ca1orimetrica11y may persist to considerably lower levels of bromination and may thus provide a more sensitive measure of segregation behavior.

Electron Microscopy Findings inN-Methyl-N-Nitrosourea-Induced Mammary Tumors

2016 ◽  
Vol 22 (5) ◽  
pp. 1056-1061
Author(s):  
Ana I. Faustino-Rocha ◽  
Ana M. Calado ◽  
Adelina Gama ◽  
Rita Ferreira ◽  
Mário Ginja ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Rat Model ◽  
Mammary Tumors ◽  
Female Rats ◽  
Ultrastructural Studies ◽  
Transmission Electron ◽  
Chemically Induced ◽  
Ultrastructural Characterization ◽  
Human Mammary Tumors

AbstractAlthough the rat model of mammary tumors chemically induced byN-methyl-N-nitrosourea (MNU) has been frequently used by several research teams, there is a lack of ultrastructural studies in this field. The main aim of this work was to perform an ultrastructural characterization of MNU-induced mammary tumors in female rats. Some alterations previously reported in human mammary tumors, such as nucleus size and shape, accumulation of heterochromatin in the perinuclear region, and interdigitating cytoplasmic processes between cancer cells were also observed in MNU-induced mammary tumors. Although a low number of samples were analyzed by transmission electron microscopy in the present study, we consider that it may contribute to a better understanding of MNU-induced mammary carcinogenesis in a rat model. The ultrastructural characteristics of the two most frequently diagnosed mammary carcinomas described in the present work can be useful to differentiate them from other histological patterns. In addition, the loss of cytoplasm in neoplastic cells and formation of vacuoles were described.

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