Ultrastructural and Scanning Electron Microscopic Studies of Basal Cell Carcinoma

1980 ◽  
Vol 38 ◽  
pp. 728-729
Author(s):  
A. Lupulescu
Keyword(s):  
Electron Microscopy ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Basal Cells ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Microscopic Studies ◽  
And Migration ◽  
Scanning Electron

Previously it has been shown that long-term topical application of 3-methylcholanthrene (MCA) on the rat skin induced basal cell carcinoma. These tumors are very similar to that occurring in humans and they were studied only by light microscopy.1 Transmission electron microscopy (TEM) and Scanning electron microscopy (SEM) can provide more characteristic details for the neoplastic transformation of basal cells, their cytoarchitecture and migration.

Scanning Electron Microscopy of Murine Oncornavirus Infected Cells

1974 ◽  
Vol 32 ◽  
pp. 252-253 ◽  
Author(s):  
S. Panem ◽  
P.S.D. Lin ◽  
A. V. Crewe ◽  
W. H. Kirsten
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscopic ◽  
Viral Budding ◽  
Transmission Electron ◽  
Virion Structure ◽  
Microscopic Studies ◽  
Infected Cells ◽  
Viral Morphogenesis ◽  
Scanning Electron

Although oncornaviruses have been the subject of numerous transmission electron microscopic studies, questions concerning virion structure and the maturation of virus at the infected cell membrane remain unanswered. We have begun to study viral morphogenesis with particular emphasis on the distribution of viral budding sites and the appearance of virus during maturation using scanning electron microscopy.

scholarly journals Scanning Electron Microscopy of Basal Cell Carcinoma

2020 ◽  
Vol 5 (1) ◽  
pp. 198-201
Author(s):  
Val閞ia M Jorge ◽  
◽  
Hiram L de Almeida Jr ◽  
Renan Pinheiro Deves ◽  
Fernando Passos da Rocha ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Scanning Electron

A Comparative Study of Fractographic Replicas

1974 ◽  
Vol 32 ◽  
pp. 566-567
Author(s):  
Loren Anderson ◽  
Pat Pizzo ◽  
Glen Haydon
Keyword(s):  
Electron Microscopy ◽  
Electron Microscopic Examination ◽  
Direct Examination ◽  
Electron Microscopic ◽  
Reliable Technique ◽  
Service Failures ◽  
Transmission Electron ◽  
Mode Of Failure ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Transmission electron microscopy of replicas has long been used to study the fracture surfaces of components which fail in service. Recently, the scanning electron microscope (SEM) has gained popularity because it allows direct examination of the fracture surface. However, the somewhat lower resolution of the SEM coupled with a restriction on the sample size has served to limit the use of this instrument in investigating in-service failures. It is the intent of this paper to show that scanning electron microscopic examination of conventional negative replicas can be a convenient and reliable technique for determining mode of failure.

Morphology and ultrastructure of oral strains of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus

1980 ◽  
Vol 30 (2) ◽  
pp. 588-600
Author(s):  
S C Holt ◽  
A C Tanner ◽  
S S Socransky
Keyword(s):  
Electron Microscopy ◽  
Ruthenium Red ◽  
Actinobacillus Actinomycetemcomitans ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Large Numbers ◽  
Haemophilus Aphrophilus ◽  
Ruthenium Red Staining ◽  
Amorphous Surface ◽  
Scanning Electron

Selected human oral and nonoral strains of the genera Actinobacillus and Haemophilus were examined by transmission and scanning electron microscopy. The strains examined were morphologically identical to recognized Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus. By transmission electron microscopy, the cells were typically gram negative in morphology, with several strains possessing some extracellular ruthenium red-staining polymeric material. Numerous vesicular structures, morphologically identical to lipopolysaccharide vesicles, were seen to originate from and be continuous with the surface of the outer membrane. Large numbers of these vesicles were also found in the external environment. Scanning electron microscopic observations revealed that both actinobacilli and haemophili possessed surface projections and an amorphous surface material which connected and covered adjacent cells.

scholarly journals A light, scanning electron and transmission electron microscopic study of a Fetal mouse Skin Dendritic Cell line (FSDC)

2009 ◽  
Vol 15 (S3) ◽  
pp. 35-36 ◽  
Author(s):  
A. M. Dinis ◽  
M. T. Cruz ◽  
M. C. Lopes ◽  
M. T. Batista
Keyword(s):  
Electron Microscopy ◽  
T Cell ◽  
Cell Line ◽  
Mouse Skin ◽  
Ultrastructural Organization ◽  
Electron Microscopic ◽  
Peripheral Tissues ◽  
Fetal Mouse ◽  
Transmission Electron ◽  
Scanning Electron

AbstractDendritic cells (DC) are major players in initiating immune responses by activating T-lymphocytes. They act as sentinels in peripheral tissues, continuously scavenging for antigens in their immediate surroundings. Their involvement in T-cell responses consists of a linear progression of events starting with capture of antigens in peripheral tissues, such as the skin, followed by migration to draining lymphoid organs and presentation of antigen-derived peptides to induce T-cell priming. In the last years, a number of DC lines have been generated and the electron microscopy has been used to define their characteristics, thus contributing to their formal validation. A cell line with features of early DC precursors was previously established from fetal mouse skin (FSDC), but its ultrastructural organization was not fully investigated. Here we report such investigation using light (LM), scanning electron (SEM) and transmission electron microscopy (TEM). The goal is to use FSDC as a model system to evaluate the safety and effectiveness of selected plant extracts, fractions and isolated polyphenols against inflammatory processes. Our preliminary results with these cells showed anti-inflammatory activity for some of the extracts we are currently investigating.

scholarly journals Use of Cytology in the Diagnosis of Basal Cell Carcinoma Subtypes

2020 ◽  
Vol 9 (3) ◽  
pp. 612
Author(s):  
Paola Pasquali ◽  
Gonzalo Segurado-Miravalles ◽  
Mar Castillo ◽  
Ángeles Fortuño ◽  
Susana Puig ◽  
...  
Keyword(s):  
Retrospective Study ◽  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Basal Cell ◽  
Inverse Correlation ◽  
Basal Cells ◽  
White Population ◽  
First Line ◽  
Common Skin ◽  
Cytological Features

Background: Basal cell carcinoma (BCC) is the most common skin cancer in the white population. Nonsurgical treatments are first-line alternatives in superficial BCC (sBCC); therefore, differentiating between sBCC and non-sBCC is of major relevance for the clinician. Scraping cytology possesses several advantages, such as an earlier diagnosis and scarring absence, in comparison to a biopsy. Nevertheless, previous studies reported difficulties in differentiating the different BCC subtypes. The objective of this study was to determine the capability and accuracy of scraping cytology to differentiate between sBCC and non-sBCC. Methods: In this retrospective study, cytological samples of histologically confirmed BCC were examined. Select cytological features were correlated to BCC subtypes (sBCC or non-sBCC). Results: A total of 84 BCC samples were included (29 sBCC; 55 non-sBCC). An inverse correlation between the diagnosis of sBCC and the presence of mucin, dehiscence, and grade of atypia in the basal cells was observed. The presence of medium and large basal cell clusters correlated directly to a sBCC diagnosis. The presence of clear cells is strongly associated with sBCC. Therefore, Conclusion: Scraping cytology is reliable in differentiating sBCC from other BCC subtypes.

scholarly journals Ultrastructural peculiarities in basal cell carcinoma

2019 ◽  
Vol 1 (1) ◽  
pp. 36-38
Keyword(s):  
Basal Cell Carcinoma ◽  
Cell Carcinoma ◽  
Tumor Cells ◽  
Basal Cell ◽  
Membrane Vesicles ◽  
Premalignant Lesions ◽  
Elastic Fibers ◽  
Cell Phenotype ◽  
Electron Microscopic ◽  
Peritumoral Stroma

The last decade was marked by a statistically significant increase in the incidence of skin cancers, which motivated the development of new studies to later understand the behavior of these pathologies developing new therapeutic approaches. Also, the multitude of premalignant lesions as well as the complex classification of the carcinomas required a more accurate differentiation of the differential diagnosis, and in this regard the present electron microscopic study contributes significantly. A tumor is a very complex ecosystem represented in particular by (1) genetically modified neoplastic cells and (2) tumor stroma represented by (a) various other cell types (fibroblasts, fibrocytes, mast cells, inflammatory cells, endothelial cells, myelinated or non-myelinated nerves, etc.), and (b) extracellular matrix (basal lamina, elastic fibers and collagen, but also soluble molecules) [1]. The purpose of this study was to discover new aspects of ultrastructure that occur in basal cell carcinoma cases investigated by us, related to the capacity of invasiveness of these tumors. Fresh tumor fragments were obtained with the informed consent of the patients. Here we present some peculiar aspects concerning infrastructure of tumor cells involved in invasive process, especially desmosomal and hemidesmosomal junctions, invadopodia and shedding membrane vesicles. Moreover, here we report about new described cell phenotype termed telocytes involved in cell signaling by their homo- and heterocellular contacts. Telocytes from basal cell carcinoma stroma exhibit a reduced number of heterocellular contacts, which suggests a possible perturbation of tissue homeostasis modulation. Electron microscopic investigations revealed that in invasive basal cell carcinoma intercellular junctions, namely desmosomes are severely altered and that the tumor cells generate and disseminate membrane vesicles, including exosomes inside of the peritumoral stroma. Using transmission electron microscopy to investigate invasive basal cell carcinoma, we have managed to determine the relevance of all these changes for the purpose of evaluation of the invasive capacity of tumor cells within the peritumoral stroma.

scholarly journals Scanning Electron Microscopic Study of Modified Chloroplasts in Senescing Broccoli Florets

HortScience ◽  
2000 ◽  
Vol 35 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Hirofumi Terai ◽  
Alley E. Watada ◽  
Charles A. Murphy ◽  
William P. Wergin
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Light Microscopy ◽  
Structural Changes ◽  
Freeze Fracture ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Scanning Electron Microscopic Study ◽  
Scanning Electron Microscopic ◽  
Scanning Electron

Structural changes in chloroplasts of broccoli (Brassica oleracea L., Italica group) florets during senescence were examined using light microscopy, scanning electron microscopy (SEM) with freeze-fracture technique, and transmission electron microscopy (TEM) to better understand the process of chloroplast degradation, particularly at the advanced stage of senescence. Light microscopy revealed that chloroplasts, which initially were intact and green, became obscure in shape, and their color faded during senescence. Small, colored particles appeared in cells as the florets approached the final stage of senescence and became full- to dark-yellow in color. Scanning electron microscopy showed that stroma thylakoids in the chloroplast initially were parallel to each other and grana thylakoids were tightly stacked. As senescence advanced, the grana thylakoids degenerated and formed globules. The globules became larger by aggregation as senescence progressed, and the large globules, called “thylakoid plexus,” formed numerous vesicles. The vesicles ultimately were expelled into the cytosol, and the light microscope revealed many colored particles in the senescent cells. These results indicate that the degradation of chloroplasts in broccoli florets progresses systematically, with the final product being colored particles, which are visible in yellow broccoli sepal cells.

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