Morphology of Abnormal Columns of Mesenchyme Cells in the Channel Catfish, Ictalurus Punctatus.

1976 ◽  
Vol 34 ◽  
pp. 182-183
Author(s):  
Vernon Henderson
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Structural Changes ◽  
Microscopic Analysis ◽  
Common Source ◽  
Brood Stock ◽  
Electron Microscopic ◽  
Fin Ray ◽  
Fin Rays ◽  
Adipose Fin

During recent years , I have utilized several thousand young channel catfish or fry for several different studies. A few years ago, I noticed an increase in the number of adipose fins which showed fin ray-like structures. Fin rays in adipose fin of fish are rare. Assuming that the said structures were fin rays, I proceeded to study them, histologically. Upon microscopic examination, it was discovered that there was a complete absence of fin ray elements. In an effort to understand the structural changes responsible for the appearance of the ray-like elements , an electron microscopic analysis was undertaken.Fish used in this study were secured from several different catfish farms located in the Grambling area; however, all seem to be linked to a common source from which brood stock had been purchased. The specimen used in this study ranged in length from 7.5 centimeters to 15.0 centimeters and were approximately four to six months in age.

Diet fed to brood stock effects the growth of channel catfish (Ictalurus punctatus L) fry

2011 ◽  
Vol 42 (12) ◽  
pp. 1899-1904 ◽  
Author(s):  
Amina Zuberi ◽  
Megan Gima ◽  
Andrew Gima ◽  
Alison Hutson ◽  
Atra Chaimongkol ◽  
...  
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Brood Stock

scholarly journals N-Acetylcysteine Attenuates Monocrotophos-induced Toxicity by Mitigating Oxidative Stress and Structural Changes in Rat Liver

2021 ◽  
Author(s):  
Jagjeet Singh ◽  
Annu Phogat ◽  
Chandra Prakash ◽  
Vijay Kumar ◽  
Vinay Malik
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Rat Liver ◽  
Protein Oxidation ◽  
Liver Tissue ◽  
Structural Changes ◽  
Microscopic Analysis ◽  
Stress Generation ◽  
Electron Microscopic ◽  
Pre Treatment

Abstract The present study evaluated the effect of N-acetylcysteine (NAC) against sub chronic monocrotophos (MCP) exposure induced oxidative stress in rat liver. Albino wistar rats were divided into control, NAC treated, MCP and MCP treated groups. An oral dose of MCP (0.9 mg/kg b.wt) and NAC (200 mg/kg b.wt) was administered for 28 days. We observed high oxidative stress generation on MCP exposure in liver tissue as evident by significant increase in lipid peroxidation, protein oxidation and decreased glutathione content followed by altered activities of superoxide dismutase, catalase and acetylcholinesterase. Sub chronic MCP exposure caused an array of cellular and structural alternations in lipids and proteins of liver tissue as depicted by the FTIR, histopathological and electron microscopic analysis. N-acetylcysteine attenuated the loss of glutathione and prevented lipid peroxidation and protein oxidation. Pre-treatment of NAC also restored histological and ultra space structural alternations. So NAC protects oxidative stress and tissue damage induced by sub chronic MCP exposure in rat liver; suggesting the therapeutic and antioxidant potential of NAC.

scholarly journals A new miniature of Xenurobryconini (Characiformes: Characidae) from the rio Tapajós basin, Brazil

2016 ◽  
Vol 14 (1) ◽  
Author(s):  
Marina Barreira Mendonça ◽  
Luiz Antônio Wanderley Peixoto ◽  
Guilherme Moreira Dutra ◽  
André Luiz Netto-Ferreira
Keyword(s):  
New Species ◽  
Posterior Half ◽  
Caudal Fin ◽  
Number Of Species ◽  
Fin Ray ◽  
Fin Rays ◽  
Dark Color ◽  
Adipose Fin ◽  
A New Species

Abstract A new species of Xenurobrycon isdescribed from tributaries of the rio Tapajós, Pará, Brazil. It is diagnosed from all congeners by the dark color of the posterior half of both caudal-fin lobes, the presence of infraorbitals one, two, and three, the presence of a set of lamellar processes on eighth principal caudal-fin ray of mature males, the presence of bony hooks similar in size or decreasing posteriorly on last unbranched and first 9-12 branched anal-fin rays of mature males, the presence of only conical teeth on both jaws, the lack of the adipose fin and the presence of 15-18 predorsal scales. The description of the new species increases the number of species in Xenurobrycon to six. An updated key to the species of the genus is provided.

Biochemical and Structural Changes in Mitochondria and Other Cellular Components of Pea Cotyledons During Germination

1972 ◽  
Vol 50 (7) ◽  
pp. 725-737 ◽  
Author(s):  
T. Solomos ◽  
S. S. Malhotra ◽  
S. Prasad ◽  
S. K. Malhotra ◽  
Mary Spencer
Keyword(s):  
Electron Microscopy ◽  
Structural Changes ◽  
Gradient Centrifugation ◽  
Microscopic Analysis ◽  
Sucrose Density Gradient ◽  
Electron Microscopic ◽  
Sucrose Density Gradient Centrifugation ◽  
Cellular Components ◽  
Further Development

Integrated studies comprising biochemical and electron microscopic analysis suggested that the increase in respiratory activity of pea cotyledon mitochondria during germination results from further development of the original mitochondria present in dormant seeds. Electron microscopy of isolated mitochondria as well as mitochondria in situ has revealed that membranes are scarce in the mitochondria present in dormant seeds. Mitochondrial cristae become well developed during the initial stages of germination. Crude mitochondrial preparations from pea cotyledons were fractionated by sucrose density gradient centrifugation and analyzed through electron microscopy. These studies showed that, at all stages of germination, "peroxisome"-like structures were present in the fractions of higher sucrose densities than that containing mitochondria. Biochemical studies revealed that the activities of catalase (H2O2:H2O2 oxidoreductase, EC 1.11.1.6) and peroxidase (guaicol:H2O2 oxidoreductase, EC 1.11.1.7) were associated mainly with these fractions and their activities increased during germination.

scholarly journals Gephyrocharax torresi (Characiformes: Characidae: Stevardiinae), a new species from the río Cascajales basin, río Magdalena system, Colombia

2013 ◽  
Vol 11 (2) ◽  
pp. 275-284 ◽  
Author(s):  
James Anyelo Vanegas-Ríos ◽  
María de las Mercedes Azpelicueta ◽  
Juan Marcos Mirande ◽  
María Dolly García Gonzales
Keyword(s):  
New Species ◽  
The Body ◽  
Pectoral Fin ◽  
Caudal Peduncle ◽  
Caudal Fin ◽  
Fin Ray ◽  
Fin Rays ◽  
Adipose Fin ◽  
Black Pigmentation ◽  
A New Species

A new species of Gephyrocharax is described from the río Cascajales basin, a tributary of the río La Colorada, río Magdalena system, Colombia. The new species is distinguished from its congeners, exceptG. melanocheir, by the absence of an adipose fin in most specimens and by the possession of a lateral branched pectoral-fin ray in males with a distal fan-shaped structure with minute bony hooks and a dark blotch or a few scattered dark brown chromatophores along its branches. The new species differs from G.melanocheir by the absence of an intense black pigmentation at the base of the anterior five dorsal-fin rays, the number of vertebrae (40-41vs. 38-39), the frontals contacting each other anterior to the epiphyseal bar in adults (vs. the absence of contact), the posterior margin of the mesethmoid straight in its central portion (vs. strongly concave at this point), the pouch scale of mature males reaching caudal-fin ray 11 or the area between caudal-fin rays 11 and 12 (vs. reaching only to caudal-fin ray 10 or the area between caudal-fin rays 9 and 10), the number of minute terminal branches of the lateral branched pectoral-fin ray of mature males (60-88 vs. 28-54), a longer black lateral stripe along the body in males (reaching to the base of the caudal-fin rays vs. reaching the middle of the length of the caudal peduncle), and the snout length (28.3-31.8% HLvs. 22.2-28.0% HL). The diagnosis ofGephyrocharax is modified to include species with the adipose fin variably present

scholarly journals IPTASE SYNTHESIS AND LIGHT MEDIATION IN TRANSGENIC TOBACCO

HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 737e-737
Author(s):  
Sandra L. Barbour ◽  
Margaret J. McMahon ◽  
John J. Frett ◽  
Dennis R. Decoteau
Keyword(s):  
Plant Growth ◽  
Transgenic Tobacco ◽  
Structural Changes ◽  
Polyclonal Antibodies ◽  
Red Light ◽  
Microscopic Analysis ◽  
Cellular Level ◽  
Cytokinin Activity ◽  
Electron Microscopic ◽  
Cytokinin Biosynthesis

Similarities exist between the effects of phytochrome and cytokinins on plant growth and development (e.g., chloroplast development, amaranthin synthesis. seed germination, photomorphogenesis). It is unclear, however, if and how these two systems interact. As a beginning step to determine cytokinin-phytochrome interactions, we developed a strategy utilizing ipt -transgenic tobacco in phytochrome/light treatment investigations. The sour-cc of the ipt gene was Agrobacterium tumefaciens Ti plasmid 15955. This gene encodes for isopentenyl transferase which is an enzyme active in cytokinin biosynthesis. Ipt -transgenic tobacco cultures (grown on MS medium supplemented with kanamycin but no plant growth regulators) were treated with end-of-day red or far-red light for 15 minutes. After 30 days of treatment, the plant tissue was harvested and either homogenized for SDS-PAGE or fixed for transmission electron microscopic analysis. Results from immuno-gold labelling using polyclonal antibodies specific to iptase will he used to Indicate the influence of phytochrome on cytokinin activity. Also, structural changes at the ultra-cellular level will be determined.

scholarly journals Urinary C-type natriuretic peptide excretion: a potential novel biomarker for renal fibrosis during aging

2011 ◽  
Vol 301 (5) ◽  
pp. F943-F952 ◽  
Author(s):  
S. Jeson Sangaralingham ◽  
Denise M. Heublein ◽  
Joseph P. Grande ◽  
Alessandro Cataliotti ◽  
Andrew D. Rule ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Renal Fibrosis ◽  
Structural Changes ◽  
Microscopic Analysis ◽  
The Elderly ◽  
Electron Microscopic ◽  
Functional Changes ◽  
Age Related ◽  
Increased Risk ◽  
Renal Aging

Renal aging is characterized by structural changes in the kidney including fibrosis, which contributes to the increased risk of kidney and cardiac failure in the elderly. Studies involving healthy kidney donors demonstrated subclinical age-related nephropathy on renal biopsy that was not detected by standard diagnostic tests. Thus there is a high-priority need for novel noninvasive biomarkers to detect the presence of preclinical age-associated renal structural and functional changes. C-type natriuretic peptide (CNP) possesses renoprotective properties and is present in the kidney; however, its modulation during aging remains undefined. We assessed circulating and urinary CNP in a Fischer rat model of experimental aging and also determined renal structural and functional adaptations to the aging process. Histological and electron microscopic analysis demonstrated significant renal fibrosis, glomerular basement membrane thickening, and mesangial matrix expansion with aging. While plasma CNP levels progressively declined with aging, urinary CNP excretion increased, along with the ratio of urinary to plasma CNP, which preceded significant elevations in proteinuria and blood pressure. Also, CNP immunoreactivity was increased in the distal and proximal tubules in both the aging rat and aging human kidneys. Our findings provide evidence that urinary CNP and its ratio to plasma CNP may represent a novel biomarker for early age-mediated renal structural alterations, particularly fibrosis. Thus urinary CNP could potentially aid in identifying subjects with preclinical structural changes before the onset of symptoms and disease, allowing for the initiation of strategies designed to prevent the progression of chronic kidney disease particularly in the aging population.

scholarly journals N-Acetylcysteine Reverses Monocrotophos Exposure-Induced Hepatic Oxidative Damage via Mitigating Apoptosis, Inflammation and Structural Changes in Rats

Antioxidants ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 90
Author(s):  
Jagjeet Singh ◽  
Annu Phogat ◽  
Chandra Prakash ◽  
Sunil Kumar Chhikara ◽  
Sandeep Singh ◽  
...  
Keyword(s):  
Antioxidant Enzyme ◽  
Inflammatory Cytokines ◽  
Rat Liver ◽  
Enzyme Activities ◽  
Structural Changes ◽  
Microscopic Analysis ◽  
Natural Antioxidants ◽  
Antioxidant Enzyme Activities ◽  
Electron Microscopic ◽  
Pro Inflammatory Cytokines

Oxidative stress-mediated tissue damage is primarily involved in hepatic injuries and dysfunctioning. Natural antioxidants have been shown to exert hepatoprotective, anti-inflammatory and antiapoptotic properties. The present study evaluated the effect of N-acetylcysteine (NAC) against monocrotophos (MCP) exposure-induced toxicity in the rat liver. Albino Wistar rats were divided into four groups: (1) control, (2) NAC-treated, (3) MCP-exposure, (4) NAC and MCP-coexposure group. The dose of MCP (0.9 mg/kg b.wt) and NAC (200 mg/kg b.wt) were administered orally for 28 days. Exposure to MCP caused a significant increase in lipid peroxidation, protein oxidation and decreased glutathione content along with the depletion of antioxidant enzyme activities. Further MCP exposure increased pro-inflammatory cytokines levels and upregulated Bax and Caspase-3 expressions. MCP exposure also caused an array of structural alternations in liver tissue, as depicted by the histological and electron microscopic analysis. Thepretreatment of NAC improved glutathione content, restored antioxidant enzyme activities, prevented oxidation of lipids and proteins, decreased pro-inflammatory cytokines levels and normalized apoptotic protein expression. Treatment of NAC also prevented histological and ultrastructural alternations. Thus, the study represents the therapeutic efficacy and antioxidant potential of NAC against MCP exposure in the rat liver.

Electron Microscopic observations of channel catfish macrophages infected with Edwardsiella ictaluri

1991 ◽  
Vol 49 ◽  
pp. 84-85
Author(s):  
L. A. Stanley
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Disease Process ◽  
Brain Heart Infusion ◽  
Edwardsiella Ictaluri ◽  
Bacterial Strains ◽  
Electron Microscopic ◽  
Susceptible Host ◽  
Virulent Strains ◽  
Infusion Agar

Edwardsiella ictaluri, a gram negative rod shaped enteric bacterium, is the etiological agent of Enteric Septicemia of Catfish (ESC). Little is known about the pathogenic mechanisms operative in ESC. It has been reported that infected macrophages are apparently unable to destroy the bacterium. The objective of this study was to detect differences in the way E. ictaluri virulent and avirulent strains associate with and are engulfed by channel catfish macrophages. This should help to characterize the possible role macrophages play in the disease process of ESC.The virulent bacterial strains used in this study were isolated from the kidneys of moribund channel catfish. Attenuated avirulent forms were derived from virulent strains by subsequent passages on Brain Heart Infusion Agar. The virulent and avirulent types were metabolically identical. Channel catfish, Ictalurus punctatus, were used as the susceptible host animals for experiments. For phagocytic studies, channel catfish macrophage suspensions were prepared as described by Trust et al.

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