Light microscopy of ceramics

Author(s):  
Walter C. McCrone

An excellent chapter on this subject by V.D. Fréchette appeared in a book edited by L.L. Hench and R.W. Gould in 1971 (1). That chapter with the references cited there provides a very complete coverage of the subject. I will add a more complete coverage of an important polarized light microscope (PLM) technique developed more recently (2). Dispersion staining is based on refractive index and its variation with wavelength (dispersion of index). A particle of, say almandite, a garnet, has refractive indices of nF = 1.789 nm, nD = 1.780 nm and nC = 1.775 nm. A Cargille refractive index liquid having nD = 1.780 nm will have nF = 1.810 and nC = 1.768 nm. Almandite grains will disappear in that liquid when observed with a beam of 589 nm light (D-line), but it will have a lower refractive index than that liquid with 486 nm light (F-line), and a higher index than that liquid with 656 nm light (C-line).

2012 ◽  
Vol 531-532 ◽  
pp. 636-639
Author(s):  
Su Feng Zhang ◽  
Chun Lei Kang

The crystal structure of aramid fibers is highly orientated. The structure of aramid fibers with various manufacturing processes and aramid paper sheets were analyzed and observed by using polarized light microscope (PLM). The change and its law of aramid fiber crystal structure in such processes as aramid fiber mamufaturing of aramid fiber/fibrids, forming of aramid paper sheets, and heat treatment were analysed. The relationship between the structural feature of aramid fibers and the performance of aramid paper was also discussed.


2021 ◽  
Author(s):  
Kiyanoush Goudarzi ◽  
Moonjoo Lee

Abstract This study demonstrates the appearance of super intense and wide Mie bandgaps in metamaterials composed of germanium rods in air that tolerate some disordering of rod position and rod radius under transverse magnetic (TM) polarized light waves. Results for Mie bandgap modes TM01 and TM11 tolerate rod-position disordering of 50%, and rod-radius disordering of 34 and 20%, respectively. Using these characteristics of TM11 under position and radius disordering, ultra-narrow straight, L-shaped and crossing waveguides that contain 14, four, and two rows of Ge rods in air are designed. Also, it is shown that TE01 Mie bandgap appear in metamaterials contain high refractive index, and disappear in metamaterials with lower refractive index such as silicon; in contrast, a new phenomenon of intense and broadband TM01, TM11, and TM21 in metamaterials with lower refractive index such as silicon appear. Also, in Si-based metamaterials, TM01 tolerates high rod-position and rod-radius disordering of 50% and 34%, respectively, and TM11 shows robustness to rod-position and rod-radius of 20%. This strong tolerance of disordering of TM modes in silicon and germanium metamaterials opens a new way to design small, high-efficient, and easy-fabricable optical devices for optical integrated circuits.


1960 ◽  
Vol s3-101 (54) ◽  
pp. 223-239
Author(s):  
K.F. A. ROSS ◽  
W.G. B. CASSELMAN

Living muscle-fibres from freshly killed mice were mounted in isotonic saline / protein media and examined with a Smith interference microscope, usually with a white light-source. When the A or I bands near the edge of a fibre were observed to match the colour of the background field, their refractive indices were close to that of the mounting medium; although it is extremely probable that diffraction at the edges of the adjacent unmatched bands affected their apparent match, so that they were not exactly of this refractive index. Matched A bands were distinguished from matched I bands by examining them in plane-polarized light, by rotating the preparation through a right angle under the interference microscope to display their birefringence, and from the colour of the unmatched bands when the fringe system of the microscope was left unaltered. In any one fibre, the refractive indices of the A-band regions were always higher than that of the I-band regions. The H bands had lower refractive indices than the A bands, and the Z bands higher than the I bands, but these were both too narrow to be matched satisfactorily by this method. The refractive indices of the solutions in which matched I bands were found ranged from 1.358 to 1.363, and those in which matched A bands were found from 1.360 to 1.366. The mean refractive index of the A and I bands was very close to 1.363, which is equivalent to a total solid content of 16% w/v. These findings are in good general agreement with those of H. Huxley and Hanson (1957) and Bennett (1955), who measured the distribution of solid material in isolated glycerinated mammalian myofibrils; but the difference between the refractive indices of the A- and I-band regions of the living fibres appeared to be very much less. Only part of this discrepancy can be accounted for by the presence of non-fibrillar solid material, because the total amount of this is extremely unlikely to exceed 50% of the total myofibrillar fibrous protein. It therefore seems probable that, because of the diffraction from the unmatched bands, the true refractive indices of the A bands were higher than those of the solutions in which they appeared matched, and those of the I bands were correspondingly lower than those of the solutions in which they appeared matched. The maximum error involved here (when the sarcomere interval was approximately 2.5 µ) can be quantified from independent estimations of the non-fibrillar material in whole muscle (Szent-Györgyi and others, 1955; Hanson and H. Huxley, 1957); and from this it seems highly probable that the refractive indices of the I bands were not lower than 1.350 (equivalent to a solid content of 9% w/v), and those of the A bands were not higher than 1.375 (equivalent to a solid content of 33% w/v).


Reproduction ◽  
2017 ◽  
Vol 153 (6) ◽  
pp. 775-784 ◽  
Author(s):  
A Bertero ◽  
F Ritrovato ◽  
F Evangelista ◽  
V Stabile ◽  
R Fortina ◽  
...  

The purpose of this study was to observe in vitro-matured equine oocytes with an objective computerized technique that involves the use of a polarized light microscope (PLM) in addition to the subjective morphological evaluation obtained using a classic light microscope (LM). Equine cumulus-oocyte complexes (COCs, n = 922) were subjected to different in vitro maturation times (24, 36 or 45 h), however, only 36-h matured oocytes were analyzed using CLM. The 36-h matured oocytes that reached maturity were parthenogenetically activated to evaluate the quality and meiotic competence. Average maturation percentages per session in groups 1, 2 and 3 (24-, 36- and 45-h matured oocytes respectively) were 29.31 ± 13.85, 47.01 ± 9.90 and 36.62 ± 5.28%, whereas the average percentages of immature oocytes per session were 28.78 ± 20.17, 7.83 ± 5.51 and 22.36 ± 8.39% respectively. The zona pellucida (ZP) birefringent properties were estimated and correlated with activation outcome. ZP thickness and retardance of the inner layer of the zona pellucida (IL-ZP) were significantly increased in immature oocytes compared with mature oocytes (P < 0.001 and P < 0.01 respectively). The comparison between parthenogenetically activated and non-activated oocytes showed a significant increase in the area and thickness of the IL-ZP in parthenogenetically activated oocytes (P < 0.01). These results show that the 36-h in vitro maturation (IVM) protocol allowed equine oocytes to reach maturity, and PLM observation of ZP can be used to distinguish mature and immature oocytes as well as activated and non-activated oocytes.


2020 ◽  
pp. 1-3
Author(s):  
Vaddadi Krishna Mohan ◽  
Ramya R ◽  
Sudha Gunasekaran

The aim of the study is to evaluate the dentinal changes in attrited and normal tooth & to correlate the relationship between attrition and age changes in dentin . In this study 30 extracted teeth were used. The teeth were categorised into two groups (attrited and normal teeth) and comparison was made between light microscope and polarised light microscopic findings. All the thirty freshly extracted teeth were fixed 10% formalin for 24-48 hours. The dentinal changes secondary to attrition such as dead tracts and dentinal sclerosis formation were studied under light microscopy and correlated with that of polarised light microscope..Samples analysed in polarised light microscope showed that dead tracts and dentinal sclerosis was highly significant (p<0.001) . The polarised light microscope is a powerful magnification tool which offers extremely high resolution.


The author begins this paper by an enumeration of the various works on the subject extant in our language, and a general mention of the writings of foreign mathematicians, which he considers as leaving room for further inquiry and simplification. He then states the method employed in his experiments for determining the refractive and relative dispersive powers of his glasses, the former of which is that generally known and practised;—of measuring the radii and focal length of a lens, and thence deriving the refractive index; with some refinements in its practical application, consisting chiefly in using the lens as the object-glass of a telescope, and adapting to it a positive eye-piece and cross-wires, which are brought precisely to the true focus by the criterion of the evanescence of parallax arising from a motion of the eye, as is practised in adjusting the stops of astronomical instruments. The only source of error it involves is in the measurement of radii of the tools which it was found could always be performed within 1/500th of their whole values. The dispersive ratio of two glasses was determined by over-correcting the dispersion of a convex lens of the less dispersive glass by a concave of the greater, and then withdrawing the latter from the former till the achromaticity is perfect, or as nearly so as the materials will admit, and measuring the interval between the lenses and their foci, from which data the ratio of their dispersive powers is easily obtained. The refractive indices and dispersive ratio thus determined, the next step is to find the radii of curvature so as to destroy spherical -aberration. In this investigation, the author does not consider it as necessary to limit the indeterminate problem by any further condition, as others before him have done, but regarding it as a matter of great convenience to avoid contact of the interior surfaces in the centre of the glasses, leaves it open to the optician to make a choice within certain limits, thus avoiding what he considers as an intricate equation arising out of the fourth condition. He proceeds, therefore, to express analytically the aberrations of the glasses, and to deduce the equation expressive of its destruction, which of course involves one indeterminate quantity; this may be either of the radii, or any combination of them. The author chooses the ratio of the radii of the interior and exterior surfaces of his flint lens for this indeterminate, which he assumes, as well as may be, to satisfy the condition of the absence of contact and near equi-curvature of the adjacent surfaces; thence deduces, first, the radii of both of the surfaces of the flint lens; next, its aberration to be corrected; and thence, by the solution of a quadratic, or by the use of a table containing its solutions registered in various states of the data, the ratio of the radii of the convex, whence the radii themselves are easily deduced.


When an object is viewed under the microscope, its visibility depends on its opacity, its colour and the extent to which it differs in its refractive index from the surrounding medium. There are a number of methods by which the contrast of a transparent object, whose refractive index differs slightly from the surrounding medium, can be enhanced. These include dark-ground illumination and, in the special case of birefringent materials, the use of polarized light. The most recent and successful addition to these methods is the phase-contrast technique which owes its inception to Zernike (1934) and has since been the subject of investigation by a number of other workers. It has occurred to me that a powerful method of increasing contrast in the case of transparent substances, in particular certain specimens of biological interest, would be provided by placing the object under investigation between two half-silvered or half-metallized surfaces which would thus constitute an interferometer. Our knowledge of the interference phenomena which occur under such conditions has been greatly enlarged in a recent series of papers by Tolansky (1943 a , b , 1944 a , b , 1945, 1946).


Author(s):  
William E. Vanderlinde ◽  
David A. Stoney

Abstract Optical microscopy techniques used by forensic analysts are shown to have application to failure analysis problems. Proper set up of the optical microscope is reviewed, including the correct use of the field diaphragm and the aperture diaphragm. Polarized light microscopy, bright and dark field methods, refractive index liquids, and a particle reference atlas are used to identify contamination found on semiconductor products.


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