scholarly journals Assessment of Brucellosis Card test in screening patients for brucellosis

1988 ◽  
Vol 100 (3) ◽  
pp. 389-398 ◽  
Author(s):  
G. F. Araj ◽  
G. M. Brown ◽  
M. M. Haj ◽  
N. V. Madhvan
Keyword(s):  
Elisa Test ◽  
Rapid Screening ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Groups ◽  
Igm Elisa ◽  
Microagglutination Test ◽  
Rapid Screening Test ◽  
Specific Igg ◽  
Card Test

SummaryThe Brucellosis Card test (Brewers' Diagnostic Kits, Hynson, Westcott and Dunning, Inc., Baltimore, Md.) was evaluated in relation to the Brucelloslide test (bioMérieux, France), the microagglutination test (MAT) and the demonstration of brucella-specific IgG, IgM and IgA in an enzyme-linked immunosorbent assay (ELISA). A total of 573 serum specimens was tested. These included sera from patients with acute brucellosis (159), chronic brucellosis (23) and patients who had been diagnosed previously as having had brucella infection (155). Control groups consisted of patients with diseases other than brucellosis (52), others with noninfectious diseases (20), and healthy individuals (164). The Card test detected 100% of the patients with acute and 61% of the patients with chronic brucellosis. The sera from the control groups were all negative. Similar results were obtained with the Brucelloslide test and the MAT. The ELISA test detected brucellaspecific Ig of all classes in the serum of patients with acute brucellosis, and IgG and IgA in the serum of patients with chronic brucellosis. In the latter group, IgM was also detected in 32% of the sera. Twenty-three per cent of sera with titres of 20 by the MAT were positive on the Card test and had ELISA titres for IgM, IgG and IgA of 400. Characterization of the antibodies involved in the Card test showed that sera with IgM ELISA titres of 1600, or an IgM titres of 800 together with IgG and IgA titres ≥ 200 were Card test positive. Higher IgG (≥ 1600) plus IgA (≥ 400) titres were required to produce a positive Card test in the absence of IgM or when the IgM titre was ≤ 200. The Card test has a potential value as a rapid screening test for humans with acute brucellosis and shows similar results to Brucelloslide and MAT tests. ELISA, however, remains the most reliable test for diagnosis of brucellosis especially in patients with chronic and complicated stages of the disease.

scholarly journals Development of a Recombinant Protein-Based Enzyme-Linked Immunosorbent Assay for Diagnosis of Mycoplasma bovis Infection in Cattle

2013 ◽  
Vol 21 (2) ◽  
pp. 196-202 ◽  
Author(s):  
Nadeeka K. Wawegama ◽  
Glenn F. Browning ◽  
Anna Kanci ◽  
Marc S. Marenda ◽  
Philip F. Markham
Keyword(s):  
Enzyme Linked Immunosorbent Assay ◽  
Mycoplasma Bovis ◽  
Western Blots ◽  
Content Type ◽  
Amino Terminal ◽  
Igm Elisa ◽  
Specific Igg ◽  
Lipase A ◽  
Strong Immunoreactivity ◽  
Gst Fusion Proteins

ABSTRACTMycoplasma boviscauses a range of diseases in cattle, including mastitis, arthritis, and pneumonia. However, accurate serological diagnosis of infection remains problematic. The studies described here aimed to identify an antigen that might be used to develop a more specific and sensitive diagnostic assay. A 226-kDa immunogenic protein was consistently detected in Western blots by antibodies in sera from calves experimentally infected withM. bovis. This protein was shown to be a membrane protein with lipase activity and was named mycoplasma immunogenic lipase A (MilA). Different regions of MilA were expressed inEscherichia colias glutathioneS-transferase (GST) fusion proteins and recombinant products from the amino-terminal end shown to have strong immunoreactivity withM. bovis-specific bovine sera. The most immunoreactive fusion protein, GST-MilA-ab, was used to develop indirect IgM and IgG enzyme-linked immunosorbent assays (ELISAs). The IgM ELISA detectedM. bovis-specific IgM antibody 2 weeks after infection with 97.1% sensitivity and had a specificity of 63.3%, while the IgG ELISA detectedM. bovis-specific IgG 3 weeks after infection with 92.86% sensitivity and had a specificity of 98.7%, demonstrating that the IgG ELISA has potential for use as a sensitive and specific assay for detecting infection in cattle.

scholarly journals Specific IgE response in patients with brucellosis

1990 ◽  
Vol 105 (3) ◽  
pp. 571-577 ◽  
Author(s):  
G. F. Araj ◽  
A. R. Lulu ◽  
M. I. Khateeb ◽  
M. Haj
Keyword(s):  
Immunosorbent Assay ◽  
Specific Ige ◽  
Blood Cultures ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Controls ◽  
Serological Markers ◽  
Microagglutination Test ◽  
Specific Igg ◽  
Ige Response

SUMMARYIn the search to find discriminative serological markers to differentiate between patients with acute brucellosis and those with chronic brucellosis, an enzyme-linked immunosorbent assay (ELISA) was used to determine and compare the brucella-specific IgE response in 80 sera from patients with acute brucellosis, 37 sera from patients with chronic brucellosis, 26 sera from patients with positive blood cultures for bacteria other than brucella and 51 sera from healthy controls. The IgE findings were compared to brucella-specific IgG, IgM, IgA and IgG1–4demonstrated by ELISA, and to microagglutination test (MAT) results. Elevated (positive) antibrucella IgE titres were detected in 89 and 81 % of sera from patients with acute and chronic brucellosis respectively. The predominant antibodies found in patients with acute brucellosis were of the IgG, IgM, IgA, IgE, IgG1and IgG3types while in chronic brucellosis IgG, IgA, IgE and IgG4were found. Although IgE can be detected in patients with brucellosis, it does not discriminate between the acute and chronic stages of the disease.

Efficiency of Two Commercial ELISA Kits Compared with the BAM Culture Method for Detecting Listeria in Naturally Contaminated Foods

1991 ◽  
Vol 74 (5) ◽  
pp. 819-821 ◽  
Author(s):  
Charles W Noah ◽  
Nora C Ramos ◽  
Virginia M Gipson
Keyword(s):  
Culture Method ◽  
Elisa Test ◽  
Food Samples ◽  
Alternative Methods ◽  
Rapid Screening ◽  
Enzyme Linked Immunosorbent Assay ◽  
Screening Methods ◽  
Test Results ◽  
Enrichment Broth ◽  
University Of Vermont

Abstract The efficiency of 2 commercial enzyme-linked Immunosorbent assay (ELISA) kite (Listeria-Tek™ and Tecra™) for detecting Listeria in naturally contaminated foods was evaluated and compared with that of the culture method described in the Bacteriological Analytical Manual (BAM). Both ELISAs use modified University of Vermont (UVM-1) medium as a primary enrichment; the BAM method uses Listeria enrichment broth. Secondary enrichments for Llsterla-Tek and Tecra, respectively, were Fraser broth and UVM-2, which contains additional acriflavln-HCI. When ELISA test results differed, secondary enrichments were tested against the other ELISA; Fraser broth was used to determine recovery rates because of Its superiority over UVM-2. Of the 178 food samples examined, the presence of Listeria was detected and culturally confirmed in 38, 37, and 40 samples by the BAM, Llsterla- Tek, and Tecra methods, respectively. Differences in results of the EUSAs compared with those of the BAM method were not statistically significant; however, differences between results of the 2 ELISA methods were significant. It was concluded that as rapid screening methods, the Llsteria-Tek and the Tecra kits qualify as alternative methods to the BAM cultural method.

scholarly journals Usefulness of IgM-ELISA test for screening of Leptospirosis in Cuba

2020 ◽  
Vol 8 (2) ◽  
Author(s):  
Ana M. Obregón ◽  
Eduardo Echevarría ◽  
Odisney Lugo ◽  
Yolaine Soto ◽  
Liliet González
Keyword(s):  
Acute Phase ◽  
Febrile Illness ◽  
High Sensitivity ◽  
Elisa Test ◽  
Reference Laboratory ◽  
Test Results ◽  
Tropical Regions ◽  
Human Leptospirosis ◽  
Igm Elisa ◽  
Microagglutination Test

Introduction: Leptospirosis is a common cause of acute febrile illness in many tropical regions of the world. Early diagnosis is essential, since untreated cases can progress rapidly and mortality rates are high in severe cases. According to the observations of the Cuban National Reference Laboratory, non-reactive serology’s are prevailing in most suspected cases of human leptospirosis. Objective: to apply the IgM-ELISA test for screening of IgM antibodies using sera from patients with the acute phase of the illness. Material and methods: in the current study, 31 pairs of sera and 140 single sera from 337 suspected patients with leptospirosis were tested by two methods, a commercial IgM-ELISA test  for Leptospira and microagglutination test (MAT). Results: IgM-ELISA test results were concordant with MAT results in 90.0% (28/31) of paired sera and 88,6% (124/140) of single sera. The following serogroups: Icterohaemorrhagiae 23,74% (18/76), Pomona 22.3% (17/76), Canicola 13.1% (10/76), and Ballum 5.2% (4/76) were the most frequently found in sera testing positive by  IgM-ELISA.  Positive IgM-ELISA sera were predominantly those taken from 5th to the 8th day of the acute phase of the illness. Some samples taken from day zero to the 28th day were also positive, suggesting a high sensitivity of this test.  Conclusion: IgM-ELISA test  is useful for screening of human leptospirosis, particularly if using sera taken from days 5-8 of surveillance, which reduce the under reporting of leptospirosis cases in Cuba.  

scholarly journals Coxsackie B Viruses and Myalgic Encephalomyelitis

1988 ◽  
Vol 81 (6) ◽  
pp. 329-331 ◽  
Author(s):  
E J Bell ◽  
R A McCartney ◽  
M H Riding
Keyword(s):  
Neutralizing Antibodies ◽  
Elisa Test ◽  
Myalgic Encephalomyelitis ◽  
Control Groups ◽  
Well Control ◽  
Igm Elisa ◽  
Combined Use ◽  
Coxsackie B Viruses ◽  
Coxsackie B

Data collected over the past 6 years suggest that Coxsackie B viruses (CBV) play an important role in myalgic encephalomyelitis (ME). Since psychological upset is a feature of this illness, 247 patients, recently admitted to a psychiatric hospital, were tested for neutralizing antibodies to CBV. A total of 12.5% had significantly raised CBV titres compared with 4–5% of ‘well’ control groups; the percentage positive was greatest (21%) in those aged 30–39 years. During 1985 and 1986 sera from 290 adults with ME were tested using the newly developed CBV IgM ELISA test; 37% were CBV IgM positive compared with 9% of 500 ‘well’ adult controls. Forty-seven children, with ME were similarly tested during this period; 38% were positive, implying recent or active CBV infection. The combined use of this ELISA test and the virus probe techniques now available should further help to elucidate the exact role of CBV in this disabling illness.

Comparison of Visual, Enzyme-Linked Immunosorbent Assay Screening, and HPLC Methods in Detecting Aflatoxin in Farmers Stock Peanut Grade Samples

1988 ◽  
Vol 15 (2) ◽  
pp. 61-63 ◽  
Author(s):  
R. J. Cole ◽  
J. W. Dorner ◽  
J. W. Kirksey ◽  
F. E. Dowell
Keyword(s):  
Immunosorbent Assay ◽  
Screening Test ◽  
High Performance ◽  
State Inspection ◽  
Rapid Screening ◽  
Federal State ◽  
Enzyme Linked Immunosorbent Assay ◽  
Inspection Method ◽  
Rapid Screening Test ◽  
Inspection Service

Abstract Grade samples from 152 lots of farmers stock peanuts were analyzed for aflatoxin by both an Enzyme-Linked Immunosorbent Assay (ELISA) rapid screening test and high performance liquid chromatography (HPLC). Results from HPLC and ELISA were compared to the results of the visual inspection method used by the Federal State Inspection Service (FSIS). The results showed 41% of the grade samples with visible Aspergillus flavus (Segregation 3) contained less than 20 ppb aflatoxin when analyzed by both ELISA and HPLC methods; 18.7% of Segregation 1 peanuts actually contained aflatoxin with a range of 26-2542 ppb. The results of ELISA and HPLC agreed in 98.6% of the composite lot analyses with the detection of 20 ppb or greater. However, the ELISA rapid screening test failed to give positive tests 12 of 13 times when the aflatoxin content was between 20-43 ppb in the component samples.

Antibody Response to Somatic Antigen of Salmonella typhi in Areas Endemic and Non-Endemic for Typhoid Fever

1985 ◽  
Vol 6 (3) ◽  
pp. 110-114 ◽  
Author(s):  
Alexander Hirschl ◽  
Gerold Stanek ◽  
Manfred L. Rotter ◽  
Pak Y. Chau ◽  
Adolf H. Niemetz
Keyword(s):  
Hong Kong ◽  
Antibody Response ◽  
Typhoid Fever ◽  
Immunosorbent Assay ◽  
Salmonella Typhi ◽  
Serological Diagnosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Somatic Antigen ◽  
Microagglutination Test ◽  
Specific Igg

AbstractIn sera obtained between the 6th and the 30th day from 16 Austrian and 26 Hong Kong patients with culturally verified typhoid fever, agglutinating antibodies (microagglutination test) at significant titers were detected in 93% of the Austrian (median titer: 640) but in only 50% of the Hong Kong patients (median titer: 240). Similar results (93% and 54% positive sera respectively) were obtained for specific IgM as assessed by the enzyme-linked immunosorbent assay (ELISA) using lipopolysaccharide of S. typhi as antigen (median relative titer: 0.32 and 0.21 respectively). In contrast, specific IgG at significant concentrations were found in only 69% of the Austrian (median relative titer: 0.16) but 88% of the Hong Kong sera (median relative titer: 0.71). The (IgM-detecting) microagglutination test may be sufficiently diagnostic for typhoid fever in nonendemic areas such as Austria. In endemic regions like Hong Kong, however, tests indicative for early specific IgG are indispensable for serological diagnosis of the disease. The ELISA proved useful and is an example for such tests.

scholarly journals Rickettsial neglected zoonoses: prevalence of scrub typhus at central Karnataka

2017 ◽  
Vol 5 (8) ◽  
pp. 3672
Author(s):  
Vinod Kumar C. S. ◽  
Satish Patil ◽  
B. S. Prasad ◽  
N. K. Kalappanavar ◽  
S. G. Jayaraj ◽  
...  
Keyword(s):  
Sensitivity And Specificity ◽  
Fever Of Unknown Origin ◽  
Scrub Typhus ◽  
Unknown Origin ◽  
Blood Parameters ◽  
Elisa Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Igm Elisa

Background: Fever of unknown Origin (FUO) has many multiple causes such as enteric fever, malaria, dengue, tuberculosis, brucellosis. But scrub typhus is less known cause in Indian scenario. The present study reports the prevalence of scrub typhus at central Karnataka and compares the sensitivity and specificity of Weil-Felix test and the IgM ELISA in the detection of infection.Methods: 368 serum samples of FUO cases were collected. Weil-Felix test was performed and also analyzed for IgM antibodies to Orienta tsutsugamushi by IgM ELISA test along with haematological and biochemical investigations.Results: Out of 368 patients of fever of unknown origin, 94 cases were positive by OXK antigens by Weil Felix test and 61 were positive by ELISA test for ST IgM antibodies. Fever was the most common clinical presentation occurring in ST IgM ELISA positive cases, followed by myalgia in 90.1% cases, headache in 77%, hepatomegaly in 65.5%, splenomegaly in 62.2% and rashes were seen in 29.5% patients. Eschar was seen in 13.1% patients, pneumonia in 3.2% and meningo-encephalitis in 1.6%. Sensitivity and specificity of WFT in relation to IgM ELISA at a titre of 160 was 81.97% and 85.67% respectively.Conclusions: With the growing number of cases detected in India, scrub typhus is fast emerging as a public health threat and also due to limited diagnostics leading to underreporting, Weil Felix test could be used in adjunct with Enzyme-linked immunosorbent assay and blood parameters in the diagnosis of rickettsial diseases.

scholarly journals Study of cutaneous manifestation of chikungunya and its serological correlation

2018 ◽  
Vol 9 (4) ◽  
pp. 137
Author(s):  
Geeta B. Shinde ◽  
Sanjay Popere
Keyword(s):  
Joint Pain ◽  
Elisa Test ◽  
Immunoglobulin M ◽  
The Body ◽  
Common Finding ◽  
Enzyme Linked Immunosorbent Assay ◽  
Chikungunya Fever ◽  
Retrospective Diagnosis ◽  
Igm Elisa ◽  
The Face

Aim: To assess the hyperpigmentation after fever and joint pain as a cutaneous marker of chikungunya fever and to assess with serological correlation.Methods: A total of 15 patients comprised of 9 males, 6 females  and  neonate have aged between 14 days to 60 years presented with the pigmentation after the fever subsided were enrolled in the study. The diagnosis of chikungunya was made by detecting virus specific IgM ELISA in the serum.Results: Serological immunoglobulin M enzyme – linked immunosorbent assay (IgM ELISA) test or chikungunya virus was positive in all the patients. Generalized dark coloured pigmentation was the most common finding after the fever subsided. On examination, out of 15 cases, in most of the cases, hyperpigmentation was observed all over the body with the facial involvement. Few cases showed pigmentation over nose, centre of upper lip, on palm, sole, eyelid, dorsum, centrofacial, reticulate pattern on the face and blotchy pigmentation.Conclusion: The presence of pigmentation after fever and joint pain helps to make a retrospective diagnosis of chikungunya fever and this may be considered as a cutaneous marker of chikungunya fever in recent past. 

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