Expression of steroidogenic enzymes and TGFβ superfamily members in follicular cells of prepubertal gilts with distinct endocrine profiles

Zygote ◽  
2021 ◽  
pp. 1-7
Author(s):  
Veronica Hoyos-Marulanda ◽  
Cristina S. Haas ◽  
Karina L. Goularte ◽  
Monique T. Rovani ◽  
Rafael G. Mondadori ◽  
...  
Keyword(s):  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Statistical Significance ◽  
Control Group ◽  
Follicular Cells ◽  
Steroidogenic Enzymes ◽  
Serum Progesterone ◽  
Growth Factor Beta ◽  
Relative Gene ◽  
Tgfβ Superfamily

Summary Regulation of the transforming growth factor beta (TGFβ) superfamily by gonadotrophins in swine follicular cells is not fully understood. This study evaluated the expression of steroidogenic enzymes and members of the TGFβ superfamily in prepubertal gilts allocated to three treatments: 1200 IU eCG at D −3 (eCG); 1200 IU eCG at D −6 plus 500 IU hCG at D −3 (eCG + hCG); and the control, composed of untreated gilts. Blood samples and ovaries were collected at slaughter (D0) and follicular cells were recovered thereafter. Relative gene expression was determined by real-time PCR. Serum progesterone levels were greater in the eCG + hCG group compared with the other groups (P < 0.01). No differences were observed in the expression of BMP15, BMPR1A, BMPR2, FSHR, GDF9, LHCGR and TGFBR1 (P > 0.05). Gilts from the eCG group presented numerically greater mean expression of CYP11A1 mRNA than in the control group that approached statistical significance (P = 0.08) and greater expression of CYP19A1 than in both the eCG and the control groups (P < 0.05). Expression of BMPR1B was lower in the eCG + hCG treatment group compared with the control (P < 0.05). In conclusion, eCG treatment increased the relative expression of steroidogenic enzymes, whereas treatment with eCG + hCG increased serum progesterone levels. Although most of the evaluated TGFβ members were not regulated after gonadotrophin treatment, the downregulation of BMPR1B observed after treatment with eCG + hCG and suggests a role in luteinization regulation.

scholarly journals Transforming growth factor beta 1 expression and inflammatory cells in tooth extraction socket after X-ray irradiation

2017 ◽  
Vol 49 (2) ◽  
pp. 87
Author(s):  
Ramadhan Hardani Putra ◽  
Eha Renwi Astuti ◽  
Rini Devijanti Ridwan
Keyword(s):  
Growth Factor ◽  
Treatment Group ◽  
Transforming Growth Factor Beta ◽  
Tooth Extraction ◽  
Transforming Growth Factor ◽  
Inflammatory Cells ◽  
Radiographic Examination ◽  
Control Group ◽  
X Ray ◽  
Growth Factor Beta

Background: Radiographic examination is often used in dentistry to evaluate tooth extraction complications. X-ray used in radiographic examination, however, has negative effects, including damage to DNA and inflammatory response during wound healing process. Purpose: This study aimed to analyze the effects of X-ray irradiation on transforming growth factor beta 1 (TGF-ß1) expression and number of inflammatory cells in tooth extraction sockets. Method: Thirty rats were divided into three groups, which consist of control group (with a radiation of 0 mSv), treatment group 1 (with a radiation of 0.08 mSv), and treatment group 2 (with a radiation of 0.16 mSv). These rats in each group were sacrificed on days 3 and 5 after treatment. Inflammatory cells which were observed in this research were PMN, macrophages, and lymphocytes. Histopathological and immunohistochemical examinations were used to calculate the number of inflammatory cells and TGF-ß1 expression. Obtained data were analyzed using SPSS 16.0 software with one way ANOVA and Tukey’s HSD tests. Result: There was no significant decrease in the number of PMN. On the other hand, there were significant decreases in the number of macrophages and lymphocytes in the sacrificed group on day-5 with the radiation of 0.16 mSv. Similarly, the most significant decreased expression of TGF-ß1 was found in the group sacrificed on day 5 with the radiation of 0.16 mSv. Conclusion: X-ray irradiation with 0.08 mSv and 0.16 mSv doses can decrease TGF-ß1 expression and number of inflammatory cells in tooth extraction sockets on day 3 and 5 post extraction.

scholarly journals Clinical Significance of Hepatocyte Growth Factor and Transforming Growth Factor-Beta-1 Levels in Assessing Disease Activity in Inflammatory Bowel Disease

2020 ◽  
Vol 2020 ◽  
pp. 1-5
Author(s):  
Rania Naguib ◽  
Wafaa Mohamed El-Shikh
Keyword(s):  
Growth Factor ◽  
Hepatocyte Growth Factor ◽  
Disease Activity ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Inflammatory Responses ◽  
Control Group ◽  
Inflammatory Bowel ◽  
Growth Factor Beta ◽  
Hepatocyte Growth

Background. Transforming growth factor-beta (TGF-β) and hepatocyte growth factor (HGF) are inflammatory cytokines which function as key regulators of immunological homeostasis and inflammatory responses. They have been linked to inflammatory bowel diseases (IBD). In this study, we aim to assess the levels of TGF-β and HGF and other inflammatory markers in patients with IBD and correlate them with the disease activity. Study Design. A cross-sectional study involving 100 patients with ulcerative colitis (UC) and 100 patients with Crohn’s disease (CD) and 50 control subjects. TGF-β and HGF levels were measured and correlated with disease activity. Results and Conclusion. Serum levels of TGF-β and HGF were significantly higher in IBD patients compared with the control group. In the UC group, the levels of HGF and TGF-β were significantly higher than in the CD group. Levels of TGF-β and HGF correlate with the activity of IBD.

scholarly journals The Effects of Salvia officinalis Gel as an Adjunct to Scaling and Root Planning in Patients with Periodontitis (Clinical and Immunological Study)

2020 ◽  
Vol 10 (02) ◽  
pp. 232-237
Author(s):  
Ismael W. Aljuboori ◽  
Maha Sh. Mahmood
Keyword(s):  
Transforming Growth Factor Beta ◽  
Chronic Periodontitis ◽  
Transforming Growth Factor ◽  
Gingival Crevicular Fluid ◽  
Test Group ◽  
Control Group ◽  
Growth Factor Beta ◽  
Crevicular Fluid ◽  
Root Planning ◽  
Scaling And Root Planning

Background: Phytotherapy is the usage of herbal species with medicinal properties for the management of various diseases. Gingivitis and periodontitis are diseases that involve the role of both the bacteria and the host immune response. Over the years, various researches have shown the importance of herbal products in the management of periodontal diseases. Aims of the study: To evaluate the efficacy of locally applied Salvia officinalis gel as adjunctive in the treatment of chronic periodontitis. Subjects and methods: Fourteen patients (10 males and 4 females) with chronic periodontitis were enrolled in the present study with total number of twenty-eight periodontal pockets utilizing a split mouth design, the pockets were divided into two groups, the test group which was treated with scaling and root planning procedure and the application of the S. officinalis gel, and the control group that treated with scaling and root planning only. Plaque index (PI) and gingival index (GI) were recorded for each site. Gingival crevicular fluid (GCF) was collected from each site by using PerioCol paper strips. The concentration of the transforming growth factor beta-1 in the gingival crevicular fluid was quantified by a high sensitivity enzyme-linked immunosorbent assay. Results: The test group demonstrated a significant reduction in GI at 1-week and 1-month after the treatment comparing to baseline (1.14 vs. 1.64, p = 0.003, and 1.21 vs. 1.64, p = 0.028, respectively), while no significant reduction in the PI at recall visits comparing to baseline. The control group demonstrated no significant reduction in PI and GI at recall visits, comparing to baseline. Both the test and control groups demonstrated no significant reduction in the transforming growth factor-beta 1 concentration in the gingival crevicular fluid at one week after the treatment comparing to baseline, while at one month after the treatment only the test group demonstrated a significant reduction comparing to baseline (3.91 vs. 9.62, p = 0.044). Conclusion: The findings of the present study indicated that the S. officinalis gel has a potential anti-inflammatory role in the treatment of chronic periodontitis by monitoring both the clinical and immunological parameters.

scholarly journals THE DILATATION OF BRAIN VENTRICLE DUE TO CONGENITAL TOXOPLASMOSIS IN MICE CORRELATED WITH APOPTOSIS BUT NOT WITH TRANSFORMING GROWTH FACTOR BETA

2018 ◽  
Vol 12 (1) ◽  
Author(s):  
Lucia Tri Suwanti ◽  
Mufasirin Mufasirin ◽  
Hani Plumeriastuti
Keyword(s):  
Growth Factor ◽  
Treatment Group ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Congenital Toxoplasmosis ◽  
Control Group ◽  
Newborn Mice ◽  
Pregnant Mice ◽  
Brain Ventricle ◽  
Growth Factor Beta

This study aimed to determine the occurences of mice brain ventricles dilatation that congenitally infected with Toxoplasma gondii (T. gondii) as a marker of hydrocephalus and cellular changes in the brain. A total of twenty pregnant mice (11.5 days pregnacy) were divided into 2 groups, which were control (P1) group and treatment (P2) group. The mice in the treatment group were infected with 101 tachyzoites of T. gondii. All mice were maintained until delivery. The newborn mice were sacrificed and their brain were removed and fixed in 10% buffered formalin to prepare histology slides with HE staining for observation of ventricular width, TUNEL assay for apoptosis observation, and immunohistochemistry for the expression of transforming growth factor beta (TGF-β) observations. The data were analyzed using t test and linear regression. The results showed that ventricular width and apoptosis index significantly increased (P<0.01) in the treatment group compared to control group, but there was no difference in the expression of TGF-β (P>0.05) in both groups. Dilatation of ventricle correlated with the apoptotic index of brain cells but did not correlated with the expression of TGF-β.

scholarly journals Increasing serum level of transforming growth factor beta 2 (TGF-β) in patients with colorectal and gastric cancer in Iraq

2013 ◽  
Vol 7 (1) ◽  
pp. 72-79
Author(s):  
Ahmed Rushdi Abdullah ◽  
Shaimaa Yousif Abdulfattah ◽  
Farah Thamer Abdullah ◽  
Ali Talib Abid ◽  
Hussam Bassim Salah
Keyword(s):  
Colorectal Cancer ◽  
Gastric Cancer ◽  
Serum Level ◽  
Cancer Patients ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Control Group ◽  
Healthy Control ◽  
Growth Factor Beta ◽  
Gastric Cancer Patients

Gastrointestinal cancers (GITs) are worldwide problem particularly in highly developed countries. In Iraq, gastric cancer(GC) is the 9th most common cancer while colorectal cancers (CRC) is considered as the 7th most common cancer among all cancer patients in both males and females. The Objective of this study was to estimate the serum level of transforming growth factor beta 2 (TGF-β) in Iraqi patients who are complying from gastric and colorectal cancers. Fifty four serum samples were collected starting from the 1st of March till the mid of May of 2011 to investigate the TGF-β serum level by using ELISA technique. Thirty eight samples were gastric (H.pylori +ve) and colorectal cancer patients (GC=17, CRC=21) while the other 16 samples considered as a healthy control group. The results showed that TGF-β serum levels of both GIT tumors were increased significantly (p<0.05) compared to the healthy control group. In conclusion, the presented study showed elevated serum level of TGF-β in Gastric cancer patients which could point out to use this elevation as a biomarker for tumor prognosis; while in colorectal cancer it may evade the immune system cancer killing mechanisms. We recommend further studies concerning the correlation between serum level of TGF-β in Gastric cancer patients with staging and grading, and more immunological techniques can be implied to know the exact immunological evading mechanism of colorectal cancer cells.

scholarly journals Levels of Apelin, Endoglin, and Transforming Growth Factor Beta 1 in Iraqi Women with Polycystic Ovary Syndrome

2020 ◽  
Vol 4 (1) ◽  
pp. 21-25
Author(s):  
Noor Alhuda K. Ibrahim ◽  
Wasnaa J. Mohammad ◽  
Sanan T. Abdawahab
Keyword(s):  
Growth Factor ◽  
Polycystic Ovary Syndrome ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Polycystic Ovary ◽  
Control Group ◽  
Model Assessment ◽  
Ovary Syndrome ◽  
Growth Factor Beta ◽  
Tgf Β1

Polycystic ovary syndrome (PCOS) is one of the most common causes of infertility in women of reproductive age. The aim of the study was to determine the level of apelin, insulin resistance (IR), transforming growth factor beta 1 (TGF-β1), and endoglin in women with polycystic ovary syndrome. Fifty PCOS patients and 40 non-PCOS infertile patients were recruited. The fasting serum levels of folliclestimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), prolactin, fasting blood glucose, insulin, and apelin at the early follicular phase were measured. Levels of apelin, LH, LH/FSH, T, and fasting insulin, as well as homeostatic model assessment of IR (HOMA-IR) in PCOS patients, were significantly higher than in the control group. Correlation analysis showed that apelin level was positively correlated with body mass index and HOMA-IR. Apelin levels and TGF-β1 were significantly increased in PCOS patients while show decrease levels of endoglin.

scholarly journals Expression of Transforming Growth Factor Beta Isoforms in Canine Endometrium with Cystic Endometrial Hyperplasia–Pyometra Complex

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1844
Author(s):  
Marta Rybska ◽  
Magdalena Woźna-Wysocka ◽  
Barbara Wąsowska ◽  
Marek Skrzypski ◽  
Magdalena Kubiak ◽  
...  
Keyword(s):  
Growth Factor ◽  
Mrna Expression ◽  
Transforming Growth Factor Beta ◽  
Endometrial Hyperplasia ◽  
Transforming Growth Factor ◽  
Control Group ◽  
Key Factor ◽  
Growth Factor Beta ◽  
Uterine Diseases ◽  
Tgf Β1

Cystic endometrial hyperplasia (CEH) and pyometra are the most frequently diagnosed uterine diseases affecting bitches of different ages. Transforming growth factor beta (TGF-β) has been classified in females as a potential regulator of many endometrial changes during the estrous cycle or may be involved in pathological disorders. The aim of this study was to determine the expression of TGF-β1, -β2 and -β3 in the endometrium of bitches suffering from CEH or a CEH–pyometra complex compared to clinically healthy females (control group; CG). A significantly increased level of TGF-β1 mRNA expression was observed in the endometrium with CEH–pyometra compared to CEH and CG. Protein production of TGF-β1 was identified only in the endometrium of bitches with CEH–pyometra. An increase in TGF-β3 mRNA expression was observed in all the studied groups compared to CG. The expression of TGF-β2 mRNA was significantly higher in CEH and lower in CEH–pyometra uteri. The results indicate the presence of TGF-β cytokines in canine endometrial tissues affected by proliferative and degenerative changes. However, among all TGF-β isoforms, TGF-β1 could potentially be a key factor involved in the regulation of the endometrium in bitches with CEH–pyometra complex.

Effects of Transforming Growth Factor-Beta 2, Interleukin 6 and Fibronectin on Corneal Epithelial Wound Healing

1998 ◽  
Vol 8 (4) ◽  
pp. 224-229 ◽  
Author(s):  
H. Er ◽  
E. Uzmez
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Control Group ◽  
Corneal Epithelial ◽  
Treatment Groups ◽  
Epithelial Wound Healing ◽  
Growth Factor Beta ◽  
Beta 2

Purpose The aim of this study was to compare the effects of topically applied transforming growth factor-beta 2 (TGF-beta 2) and interleukin 6 (IL-6), alone and combined with fibronectin, on the rate of corneal wound healing in rabbits. Methods Twenty-eight rabbits were used for the experiment. After the right eye of each rabbit was debrided with n-heptyl alcohol, the animals were divided into four treatment groups (six rabbits per group) and one control group (four rabbits). The debrided eyes were treated, beginning immediately after wounding and continuing every 2 hours from 8 a.m. to 8 p.m. for 48 hours. Group 1 received TGF-beta 2; group 2 IL-6; group 3, TFR-beta 2 and purified fibronectin; group 4, IL-6 and fibronectin; control group, balanced salt solution. At set intervals each eye was stained with fluorescein and photographed; epithelial defects were measured with a computer-assisted digitizer. The healing rate was calculated by linear regression analysis. Results The mean healing rates in groups 1, 2, 3, 4, and controls were respectively 1.65 ± 0.16, 1.68 ± 0.11, 1.99 ± 0.12, 2.23 ± 0.09, and 0.93 ± 0.18 mm2/h. Mean epithelial healing rates for all drug-treatment groups were significantly faster than controls. The healing rates of groups 3 and 4 were significantly faster than groups 1 and 2. Conclusions We conclude that cytokines, in combination with extracellular matrix proteins, facilitate corneal epithelial wound healing in vivo, possibly by making corneal epithelial cells more sensitive to fibronectin receptors.

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