scholarly journals Leaf trichomes of Portuguese Lavandula species: a comparative morphological study

2009 ◽  
Vol 15 (S3) ◽  
pp. 37-38 ◽  
Author(s):  
M. R. Zuzarte ◽  
A. M. Dinis ◽  
J. Canhoto ◽  
L. Salgueiro
Keyword(s):  
Morphological Study ◽  
Glandular Trichomes ◽  
Economic Value ◽  
Aromatic Plants ◽  
Aerial Parts ◽  
Different Types ◽  
Leaf Trichome ◽  
Trichome Morphology ◽  
High Diversity ◽  
Scanning Electron

AbstractLavandula species are aromatic plants that produce and accumulate essential oils in different types of glandular trichomes. The oils protect the aerial parts of the plant against herbivores and pathogens, and are of great economic value for fragrance, pharmaceutical, food and flavour industries. In Portugal there are five spontaneous species of Lavandula which are included in three different sections: Section Lavandula (L. latifolia), Section Pterostoechas (L. multifida) and Section Stoechas (L. luisieri, L. pedunculata, L. viridis). Our preliminary observations showed that these species have a high diversity of trichomes. This, associated with the fact that trichome morphology is often referred as an useful characteristic for systematic purposes, led us to investigate leaf trichome morphology in the five Portuguese species, using scanning electron microscopy (SEM).

scholarly journals Prevalence of gastrospirillum-like organisms in pigs, cattle, and dogs: a comparison of diagnostic methods between species

2008 ◽  
Vol 53 (No. 4) ◽  
pp. 193-202 ◽  
Author(s):  
L. Kolodzieyski ◽  
B. Kim ◽  
H. Park ◽  
H.S. Yoon ◽  
C.W. Lim
Keyword(s):  
Electron Microscopy ◽  
Morphological Study ◽  
Light And Electron Microscopy ◽  
Diagnostic Methods ◽  
Brush Cytology ◽  
Specimen Collection ◽  
Urease Test ◽  
Different Types ◽  
Typical Morphology ◽  
Scanning Electron

A survey was conducted to determine the prevalence of spiral-shaped bacteria in animals as a possible source of pathogens causing chronic changes in the human and animal stomach as well as in other parts of the digestive tract. This study was carried out in three different groups of animals, pigs, cattle and dogs. Swabs from the oral cavity of dogs (<I>n</I> = 198) were stained using Gram’s method to evaluate gastrospirillum-like organisms (GLOs) and revealed two different types of GLOs with an incidence of 23.2% (46/198). The stomachs of the pigs (<I>n</I> = 104), cattle (<I>n</I> = 102), and dogs (<I>n</I> = 7) were collected for the urease test, brush cytology, light and electron microscopy, and PCR. A positive urease test was observed in 31.7% (33/104) of pigs, 90.2% (92/102) of cattle, and 85.7% (6/7) of dog samples. GLOs were detected in 37.5% (39/104) of pigs, 62.7% (64/102) of cattle, and 85.7% (6/7) of dog samples by brush cytology. Furthermore, positive PCR results were obtained in the stomach samples of dogs that had tested positive by both the urease test and brush cytology. The morphological study using brush cytology and scanning electron microscopy of a pig stomach revealed bacteria with the typical morphology of GLOs, which appeared to be similar to <I>Helicobacter heilmanii</I>. This study indicates that the urease test and brush cytology are useful tools for diagnosing GLOs in different animals. Moreover, the location of specimen collection can influence the diagnostic sensitivity of the examination.

Leaflet blade epidermis and its taxonomic significance in 13 species of Bignonieae (Bignoniaceae) from Pico do Jabre, Paraíba, Northeast of Brazil

Botany ◽  
2020 ◽  
Author(s):  
Rafael Francisco Lopes-Silva ◽  
Anauara Lima Silva ◽  
Ednalva Alves Vital dos Santos ◽  
Maria de Fátima Agra
Keyword(s):  
Glandular Trichomes ◽  
Terminal Leaflet ◽  
Epicuticular Waxes ◽  
Taxonomic Significance ◽  
Anticlinal Wall ◽  
Additional Tool ◽  
Different Types ◽  
Pyrostegia Venusta ◽  
Vegetative Characters ◽  
Scanning Electron

Bignonieae is the largest tribe of Bignoniaceae, with 21 genera and 393 species of lianas and shrubs, 2–3-foliolate with the terminal leaflet modified as tendrils. We examined the micromorphologies of the leaflet blade epidermises of 13 species of Bignonieae belonging to Amphilophium, Anemopaegma, Bignonia, Cuspidaria, Dolichandra, Fridericia, Pyrostegia, Tanaecium, and Xylophragma, from Pico do Jabre, Paraíba, Brazil. These are lianas except for Tanaecium parviflorum (shrubby). We sought to identify epidermal leaflet parameters to support their taxonomy subject to great similarities between their vegetative characters, mainly in species of the same genus and related genera. Analyses were performed using light and scanning electron microscopy, and showed five types of epicuticular waxes, four cuticle types, three epidermal cell anticlinal wall types, and non-glandular and glandular trichomes. Hypostomatic leaves showed ten different types of stomata, with stomatal indices from 6.21% (Bignonia ramentacea) to 23.52% (Tanaecium parviflorum) and stomatal densities from 76 stomata/mm² (Pyrostegia venusta) to 752.9 (T. parviflorum). The presence of raphides in Amphilophium crucigerum and styloids in Fridericia pubescens constitute the first records for these genera. Epidermal micromorphology provided a set of distinctive characters to separate these species, representing an additional tool to support their taxonomies, as well as that of tribe Bignonieae.

scholarly journals Trichomes of Satureja horvatii Silic (Lamiaceae): Micromorphology and histochemistry

2012 ◽  
Vol 64 (3) ◽  
pp. 995-1000 ◽  
Author(s):  
Marija Marin ◽  
Lia Ascensao ◽  
Branislava Lakusic
Keyword(s):  
Electron Microscopy ◽  
Leaf Surface ◽  
Glandular Trichomes ◽  
Secondary Compounds ◽  
Endemic Plant ◽  
Histochemical Analysis ◽  
Different Types ◽  
Histochemical Tests ◽  
Scanning Electron ◽  
Comprehensive Study

Considering the importance of Satureja horvatii Silic as an endemic plant, and due to the essential oils produced in its glandular trichomes, we have done a comprehensive study of the micromorphology and a histochemical analysis of the plant?s glandular trichomes. This investigation was carried out using light microscopy and scanning electron microscopy. Non-glandular unbranched and three types of glandular trichomes on the leaf surface - peltate, capitate and digitiform - were investigated. The results of histochemical tests showed a positive reaction to polysaccharides, proteins, pectins, lipids and to some secondary compounds such as terpenes, tannins and phenols in different types of glandular trichomes.

scholarly journals Trichomes and chemical composition of the volatile oil of Trichogonia cinerea (Gardner) R. M. King & H. Rob. (Eupatorieae, Asteraceae)

2016 ◽  
Vol 88 (1) ◽  
pp. 309-322 ◽  
Author(s):  
YANNE S. FERNANDES ◽  
LUMA M.P. TRINDADE ◽  
MARIA HELENA REZENDE ◽  
JOSÉ R. PAULA ◽  
LETÍCIA A. GONÇALVES
Keyword(s):  
Light Microscopy ◽  
Glandular Trichomes ◽  
Volatile Oil ◽  
Butylated Hydroxytoluene ◽  
Campo Rupestre ◽  
Volatile Oils ◽  
Aerial Parts ◽  
Scanning Electron

Trichogonia cinerea is endemic to Brazil and occurs in areas of cerrado and campo rupestre. In this study, we characterized the glandular and non-glandular trichomes on the aerial parts of this species, determined the principal events in the development of the former, and identified the main constituents of the volatile oil produced in its aerial organs. Fully expanded leaves, internodes, florets, involucral bracts, and stem apices were used for the characterization of trichomes. Leaves, internodes, florets, and involucral bracts were examined by light microscopy and scanning electron microscopy, whereas stem apices were examined only by light microscopy. Branches in the reproductive phase were used for the extraction and determination of the composition of the volatile oil. The species has three types of glandular trichomes, biseriate vesicular, biseriate pedunculate, and multicellular uniseriate, which secrete volatile oils and phenolic compounds. The major components identified in the volatile oil were 3,5-muuroladiene (39.56%) and butylated hydroxytoluene (13.07%).

scholarly journals Analysis of trichome morphology and density in Salvia nemorosa L. (Lamiaceae) of Iran

Botanica ◽  
2018 ◽  
Vol 24 (1) ◽  
pp. 49-58 ◽  
Author(s):  
Seyed Mehdi Talebi ◽  
Majid Mahdiyeh ◽  
Majid Ghorbani Nohooji ◽  
Mahsa Akhani
Keyword(s):  
Essential Oil ◽  
Light Microscopy ◽  
Glandular Trichomes ◽  
Ecological Factors ◽  
Modern Medicine ◽  
Upgma Tree ◽  
Ecological Parameters ◽  
Leaf Trichome ◽  
Trichome Types ◽  
Trichome Morphology

AbstractSalvia nemorosa L. is an aromatic herb distributed widely in various regions of Iran, and is used in traditional and modern medicine. In the present study, leaf trichome densities and also type were investigated in six populations of the species, because the effective substances of this species exist in essential oil of glandular trichomes. For this, the mature intact leaves of each population were fixed in FAA solution, and then transverse hand sections were double-stained and studied using light microscopy. Leaf indumentum in all of the populations was dense pilose, with the exception of Tehran population that had loose pilose. Thirteen kinds of trichomes were identified, the main of which were peltate and capitate. PCA-biplot showed that each of these populations had prominent trichome trait(s). Furthermore, significant positive/negative correlations were found between some trichome types with main ecological factors of habitat. The studied populations clustered separately in the UPGMA tree, moreover, PCA and also PCO plots produced similar results. Our findings confirmed that ecological parameters of a habitat have strong effects on trichome morphology and density.

Techniques for preparing the Lolium perenne coleorhiza for scanning electron microscope evaluation

1992 ◽  
Vol 50 (1) ◽  
pp. 766-767
Author(s):  
Susan B.G. Debaene ◽  
John S. Gardner ◽  
Phil S. Allen
Keyword(s):  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Lolium Perenne ◽  
Morphological Study ◽  
Root Hairs ◽  
Inner Pressure ◽  
Water Potentials ◽  
Radicle Emergence ◽  
Standard Fixation ◽  
Scanning Electron

The coleorhiza is a nonvascular sheath that encloses the embryonic radicle in Poaceae, and is generally the first tissue to emerge during germination. Delicate hairlike extensions develop from some coleorhiza cells prior to radicle emergence. Similar to root hairs, coleorhiza hairs are extremely sensitive to desiccation and are damaged by exposure to negative water potentials. The coleorhiza of Lolium perenne is somewhat spherical when first visible, after which a knob forms at a right angle to the caryopsis due to inner pressure from the elongating radicle. This knob increases in length until the radicle finally punctures the coleorhiza. Standard fixation procedures cause severe desiccation of coleorhiza cells and hairs, making morphological study of the coleorhiza difficult. This study was conducted to determine a more successful process for coleorhiza preservation.

Scanning electron microscopy of asbestos-containing material where the asbestos fibers are considered locked in a binder

1993 ◽  
Vol 51 ◽  
pp. 472-473
Author(s):  
J. R. Millette ◽  
R. S. Brown
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Environmental Protection Agency ◽  
Building Materials ◽  
The United States ◽  
Protection Agency ◽  
Asbestos Fibers ◽  
Different Types ◽  
Binder Material ◽  
Scanning Electron

The United States Environmental Protection Agency (EPA) has labeled as “friable” those building materials that are likely to readily release fibers. Friable materials when dry, can easily be crumbled, pulverized, or reduced to powder using hand pressure. Other asbestos containing building materials (ACBM) where the asbestos fibers are in a matrix of cement or bituminous or resinous binders are considered non-friable. However, when subjected to sanding, grinding, cutting or other forms of abrasion, these non-friable materials are to be treated as friable asbestos material. There has been a hypothesis that all raw asbestos fibers are encapsulated in solvents and binders and are not released as individual fibers if the material is cut or abraded. Examination of a number of different types of non-friable materials under the SEM show that after cutting or abrasion, tuffs or bundles of fibers are evident on the surfaces of the materials. When these tuffs or bundles are examined, they are shown to contain asbestos fibers which are free from binder material. These free fibers may be released into the air upon further cutting or abrasion.

scholarly journals Introgression of the sesquiterpene biosynthesis from Solanum habrochaites to cultivated tomato offers insights into trichome morphology and arthropod resistance

Planta ◽  
2021 ◽  
Vol 254 (1) ◽  
Author(s):  
Rodrigo Therezan ◽  
Ruy Kortbeek ◽  
Eloisa Vendemiatti ◽  
Saioa Legarrea ◽  
Severino M. de Alencar ◽  
...  
Keyword(s):  
Glandular Trichomes ◽  
Pest Resistance ◽  
Chromosome 8 ◽  
Cavity Size ◽  
Solanum Habrochaites ◽  
Genetic Components ◽  
Internal Storage ◽  
In The Wild ◽  
Low Levels ◽  
Trichome Morphology

Abstract Main conclusion Cultivated tomatoes harboring the plastid-derived sesquiterpenes from S. habrochaites have altered type-VI trichome morphology and unveil additional genetic components necessary for piercing-sucking pest resistance. Abstract Arthropod resistance in the tomato wild relative Solanum habrochaites LA1777 is linked to specific sesquiterpene biosynthesis. The Sesquiterpene synthase 2 (SsT2) gene cluster on LA1777 chromosome 8 controls plastid-derived sesquiterpene synthesis. The main genes at SsT2 are Z-prenyltransferase (zFPS) and Santalene and Bergamotene Synthase (SBS), which produce α-santalene, β-bergamotene, and α-bergamotene in LA1777 round-shaped type-VI glandular trichomes. Cultivated tomatoes have mushroom-shaped type-VI trichomes with much smaller glands that contain low levels of monoterpenes and cytosolic-derived sesquiterpenes, not presenting the same pest resistance as in LA1777. We successfully transferred zFPS and SBS from LA1777 to cultivated tomato (cv. Micro-Tom, MT) by a backcrossing approach. The trichomes of the MT-Sst2 introgressed line produced high levels of the plastid-derived sesquiterpenes. The type-VI trichome internal storage-cavity size increased in MT-Sst2, probably as an effect of the increased amount of sesquiterpenes, although it was not enough to mimic the round-shaped LA1777 trichomes. The presence of high amounts of plastid-derived sesquiterpenes was also not sufficient to confer resistance to various tomato piercing-sucking pests, indicating that the effect of the sesquiterpenes found in the wild S. habrochaites can be insect specific. Our results provide for a better understanding of the morphology of S. habrochaites type-VI trichomes and paves the way to obtain insect-resistant tomatoes.

scholarly journals Assigning a Value to Volunteering in Requests for Proposals

SAGE Open ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 215824402110095
Author(s):  
Jakub Dostál
Keyword(s):  
Czech Republic ◽  
Theoretical Basis ◽  
Economic Value ◽  
European Economic Area ◽  
The Czech Republic ◽  
A Value ◽  
Economic Area ◽  
Different Types ◽  
The Relationship ◽  
Volunteer Time

The economic value of volunteering is an increasingly important part of volunteering management. It has become part of public policies. Some requests for proposals (RFP) enable nonprofits to include the value of volunteer time in compulsory co-financing. These RFP include the European Economic Area (EEA) Grants and Norway Grants. This article addresses the relationship in the value of volunteering, also called in-kind volunteering contributions. The research includes two case studies of finances from EEA and Norway Grants in the Czech Republic: the Czech NGO Programme, responsible for allocating grants between 2009 and 2014, and the Active Citizens Fund, responsible for allocating grants between 2014 and 2021. They share elements through the EEA and Norway Grants rules. However, they use different types of specialist replacement wages. The article summarizes the arguments for including in-kind volunteering contributions. It presents the possible values of these contributions in the selected cases, including the relationship between the type of volunteering and the number of hours necessary to achieve these values. The article defines the theoretical basis for calculating the value of in-kind volunteer contributions and illustrates this with real examples of allocations from EEA and Norway Grants.

scholarly journals Unraveling the fine-tuned lemon coloration of a pierid butterfly Catopsilia pomona

Microscopy ◽  
2017 ◽  
Vol 66 (6) ◽  
pp. 414-423
Author(s):  
Monalisa Mishra ◽  
Ashutosh Choudhury ◽  
P Sagar Achary ◽  
Harekrushna Sahoo
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Variable Temperature ◽  
Wing Pattern ◽  
Physical Composition ◽  
Different Types ◽  
Microscopy Techniques ◽  
Pierid Butterfly ◽  
Best Fit ◽  
Scanning Electron

Abstract Butterflies wings possess different types of scales to perform diverse functions. Each scale has many nano and microstructures, which interferes with light, resulting in unique coloration for each butterfly. Besides coloration, the arrangement of scales further helps in giving better survivability. Thus, analysis of wing pattern provides an overall idea about adaptation and activity of the animal. The current study deciphers the structure and composition of a wing of a pierid butterfly Catopsilia pomona, which remains active at 42°C at which temperature all other butterflies face a tougher task for existence. In order to know the relation between survivability and adaptation in the wing, we have investigated the structural and physical composition of the wing of C. pomona under optical spectroscopy (absorption, reflectance and transmittance) along with microscopy techniques (optical and scanning electron microscopy), which are not described in earlier studies. The current findings reveal unique structural arrangement within scales to provide the best fit to the animal in variable temperature.

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