scholarly journals Analysis of the Michaelis-Menten mechanism in an immobilised enzyme reactor

2005 ◽  
Vol 47 (2) ◽  
pp. 173-184 ◽  
Author(s):  
M. I. Nelson ◽  
X. D. Chen ◽  
M. J. Sexton
Keyword(s):  
Flow Reactor ◽  
Reaction Vessel ◽  
High Flow ◽  
Operating Conditions ◽  
Pollutant Emissions ◽  
Low Flow ◽  
Enzyme Reactor ◽  
Flow Rates ◽  
Periodic Behaviour ◽  
Enzyme Species

AbstractWe investigate the behaviour of a reaction described by Michaelis-Menten kinetics in an immobilised enzyme reactor (IER). The IER is treated as a well-stirred flow reactor, with the restriction that bounded and unbounded enzyme species are constrained to remain within the reaction vessel. Our aim is to identify the best operating conditions for the reactor.The cases in which an iminobilised enzyme reactor is used to either reduce pollutant emissions or to synthesise a product are considered. For the former we deduce that the reactor should be operated using low flow rates whereas for the latter high flow rates are optimal. It is also shown that periodic behaviour is impossible.

Analysis of an Immoblised Enzyme Reactor with Catalysts Activation

2010 ◽  
Vol 4 (3) ◽  
Author(s):  
Mark I Nelson ◽  
Harvinder S Sidhu ◽  
Adesoji A Adesina
Keyword(s):  
Steady State ◽  
Immobilized Enzyme ◽  
Flow Reactor ◽  
Reaction Vessel ◽  
Operating Conditions ◽  
Residence Times ◽  
Enzyme Reactor ◽  
Steady State Analysis ◽  
Product Species ◽  
Enzyme Species

We investigate the behavior of a reaction described by Michaelis-Menten kinetics in an immobilized enzyme reactor (IER). The IER is treated as a well stirred flow reactor, in which the immobilized bounded and unbounded enzyme species are constrained to remain within the reaction vessel. The product species leaves the IER in the reactor outflow. Before the substrate can react with the enzyme, the enzyme must first be activated by absorption of an activator. We use steady state analysis to identify the best operating conditions or the reactor. To this end, we show that the concentration of product is maximized at low residence time whereas the productivity of the reactor is maximized at high residence times.

Experimental Investigation of Pressure Fluctuations on Inner Wall of a Centrifugal Pump

2019 ◽  
Vol 36 (4) ◽  
pp. 401-410 ◽  
Author(s):  
Xiao-Qi Jia ◽  
Bao-Ling Cui ◽  
Zu-Chao Zhu ◽  
Yu-Liang Zhang
Keyword(s):  
Centrifugal Pump ◽  
Flow Rate ◽  
Pressure Fluctuation ◽  
Pressure Fluctuations ◽  
High Flow ◽  
Low Flow ◽  
Centrifugal Pumps ◽  
Flow Rates ◽  
Pressure Distributions ◽  
Blade Passing Frequency

Abstract Affected by rotor–stator interaction and unstable inner flow, asymmetric pressure distributions and pressure fluctuations cannot be avoided in centrifugal pumps. To study the pressure distributions on volute and front casing walls, dynamic pressure tests are carried out on a centrifugal pump. Frequency spectrum analysis of pressure fluctuation is presented based on Fast Fourier transform and steady pressure distribution is obtained based on time-average method. The results show that amplitudes of pressure fluctuation and blade-passing frequency are sensitive to the flow rate. At low flow rates, high-pressure region and large pressure gradients near the volute tongue are observed, and the main factors contributing to the pressure fluctuation are fluctuations in blade-passing frequency and high-frequency fluctuations. By contrast, at high flow rates, fluctuations of rotating-frequency and low frequencies are the main contributors to pressure fluctuation. Moreover, at low flow rates, pressure near volute tongue increases rapidly at first and thereafter increases slowly, whereas at high flow rates, pressure decreases sharply. Asymmetries are observed in the pressure distributions on both volute and front casing walls. With increasing of flow rate, both asymmetries in the pressure distributions and magnitude of the pressure decrease.

Leach Behavior of SRL TDS-131 Defense Waste Glass in Water at High&Low Flow Rates

1983 ◽  
Vol 26 ◽  
Author(s):  
Aaron Barkatt ◽  
William Sousanpour ◽  
Alisa Barkatt ◽  
Morad A. Boroomand ◽  
Pedro B. Macedo
Keyword(s):  
Contact Time ◽  
Protective Layer ◽  
High Flow ◽  
Low Flow ◽  
Matrix Elements ◽  
Flow Rates ◽  
Low Concentrations ◽  
Controlling Mechanism ◽  
The Matrix ◽  
Leach Behavior

ABSTRACTLeach tests carried out on SRL TDS-131 Defense Waste Class indicate that at high flow rates the controlling mechanism is simple corrosion. The matrix elements (Si, Al) are leached out at rates similar to those of the leaching of the alkalis and of boron, and the leaching process is nearly linear with time. At slow flow rates (below 1 m/yr) leaching becomes controlled by the build-up of a protective layer. Al and most of the Si remain in the leached surface layer. The leach rates decrease in the course of the test before leveling off at constant values which are almost inversely proportional to the contact time, indicating that leachate concentrations have become solubility-limited. The low concentrations observed at this stage indicate the formation of alteration products.

scholarly journals An In Vitro Evaluation of the Red Cell Damage and Hemocompatibility of Different Central Venous Catheters

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Alojz Ihan ◽  
Stefan Grosek ◽  
David Stubljar
Keyword(s):  
Blood Cell ◽  
Flow Rate ◽  
Blood Cells ◽  
Cell Damage ◽  
Central Venous Catheters ◽  
High Flow ◽  
High Flow Rate ◽  
Low Flow ◽  
Central Venous ◽  
Flow Rates

Background. The aim of our study was to evaluate the damaging impact of characteristics of the central venous catheters (CVCs) on red blood cells. Methods. CVCs from three different manufacturers were analyzed, including the presence of coating, tunnel geometry, length, lumen diameter, and number of lumens with two respective flow rates (33 mL/min and 500 mL/min). Blood cell damage was defined by analyzing microparticle (MP) and hematologic analysis. MPs were isolated by ultracentrifugation of erythrocyte concentrate and analyzed on a flow cytometer. Results. Characteristics of catheters were not associated with blood cell damage at a low flow rate but showed an effect with a high flow rate. CVCs with a polyhexanide methacrylate coating have caused statistically less blood cell damage than noncoated CVCs. The length of lumens, diameter, and geometry of the tunnel showed no differences in blood cell damage. Meanwhile, the number of lumens was predicted to have a greater effect on the erythrocyte damage, which was revealed with the formation of MPs and hematological parameters. CVCs with five lumens caused significantly less damage to the blood cells than CVCs with a single lumen. Moreover, a high flow rate of 500 mL/min caused less damage to the blood cells than a low rate of 33 mL/min. Conclusion. Properties of CVCs are an important factor for quality patient care, especially when transfusing blood with high flow rates, as we want to provide a patient with high-quality blood with as few damaged cells as possible.

Rheological Effects of Blood in a Nonplanar Distal End-to-Side Anastomosis

2008 ◽  
Vol 130 (5) ◽  
Author(s):  
Qian-Qian Wang ◽  
Bao-Hong Ping ◽  
Qing-Bo Xu ◽  
Wen Wang
Keyword(s):  
Flow Rate ◽  
Stokes Equations ◽  
Bypass Graft ◽  
High Flow ◽  
Secondary Flows ◽  
Newtonian Fluids ◽  
Low Flow ◽  
Flow Rates ◽  
Flow Recirculation ◽  
Low Flow Rate

This study investigates rheological effects of blood on steady flows in a nonplanar distal end-to-side anastomosis. The shear-thinning behavior of blood is depicted by a Carreau–Yasuda model and a modified power-law model. To explore effects of nonplanarity in vessel geometry, a curved bypass graft is considered that connects to the host artery with a 90deg out-of-plane curvature. Navier–Stokes equations are solved using a finite volume method. Velocity and wall shear stress (WSS) are compared between Newtonian and non-Newtonian fluids at different flow rates. At low flow rate, difference in axial velocity profiles between Newtonian and non-Newtonian fluids is significant and secondary flows are weaker for non-Newtonian fluids. At high flow rate, non-Newtonian fluids have bigger peak WSS and WSS gradient. The size of the flow recirculation zone near the toe is smaller for non-Newtonian fluids and the difference is significant at low flow rate. The nonplanar bypass graft introduces helical flow in the host vessel. Results from the study reveal that near the bed, heel, and toe of the anastomotic junction where intimal hyperplasia occurs preferentially, WSS gradients are all very big. At high flow rates, WSS gradients are elevated by the non-Newtonian effect of blood but they are reduced at low flow rates. At these locations, blood rheology not only affects the WSS and its gradient but also secondary flow patterns and the size of flow recirculation near the toe. This study reemphasizes that the rheological property of blood is a key factor in studying hemodynamic effects on vascular diseases.

Unsteady Flow Pattern Characteristics Downstream of a Forward-Curved Blades Centrifugal Fan

2000 ◽  
Vol 123 (2) ◽  
pp. 265-270 ◽  
Author(s):  
Sandra Velarde-Sua´rez ◽  
Rafael Ballesteros-Tajadura ◽  
Carlos Santolaria-Morros ◽  
Jose´ Gonza´lez-Pe´rez
Keyword(s):  
Operating Conditions ◽  
The Other ◽  
Low Flow ◽  
Centrifugal Fan ◽  
Hot Wire ◽  
Measured Velocity ◽  
Flow Rates ◽  
Velocity Fluctuations ◽  
Flow Asymmetry ◽  
Steady Velocity

The results of an experimental investigation of the flow at two exit radial locations of a forward-curved blades centrifugal fan are presented. Hot wire techniques were used to obtain steady velocity components and velocity unsteadiness levels (rms value of the components of velocity fluctuation) for different operating conditions. Globally speaking, the data reveal a strong flow asymmetry, with considerable changes in both magnitude and direction along the different circumferential positions. Particularly, big differences appear between the circumferential positions closer to the volute tongue and the other ones. The periodic character of the velocity signals due to the passing of the blades, clearly observed around the impeller, is missed in the vicinity of the volute tongue, where the main contribution to the velocity fluctuations appears to be random. Based on the measured velocity signals, velocity unsteadiness of the flow is determined analyzing the main contributions as a function of the flow rate and the measurement position. High levels of velocity unsteadiness were observed near the volute tongue, mainly at low flow rates.

Control of Self-Excited Flow Oscillations in Vaneless Diffuser of Centrifugal Compression Systems

1982 ◽  
Author(s):  
A. N. Abdelhamid
Keyword(s):  
Flow Field ◽  
Performance Improvement ◽  
High Flow ◽  
Low Flow ◽  
Flow Oscillation ◽  
Vaneless Diffuser ◽  
Flow Area ◽  
Flow Measurements ◽  
Flow Rates ◽  
Flow Oscillations

Experiments were conducted to evaluate the effectiveness of axisymmetric diffuser exit throttle in delaying the occurrence of self-excited flow oscillation in vaneless diffusers. Sharp edge rings were installed at diffuser exit in order to change the exit flow area. Tests were carried out with the rings attached to one or both of the diffuser walls. Steady and unsteady flow measurements were used to determine the flow field in the diffuser at the onset of the flow oscillations. Results showed that the occurrence of flow oscillation was continuously delayed as the diffuser exit flow area was reduced for all these configurations and impeller speeds. Comparison between the performance of the compression system with and without diffuser exit blockage indicated that although large losses occur at high flow rates, the use of diffuser exit rings resulted in overall diffuser performance improvement at low flow rates. Retractable diffuser exit rings would therefore be ideal for centrifugal compression systems with vaneless diffuser.

scholarly journals Investigation of the Flow Field in the Vicinity of an Axial Flow Fan During Low Flow Rates

2014 ◽  
Author(s):  
Francois G. Louw ◽  
Theodor W. von Backström ◽  
Sybrand J. van der Spuy
Keyword(s):  
Flow Field ◽  
Numerical Simulations ◽  
Flow Rate ◽  
Axial Flow ◽  
Operating Conditions ◽  
Design Flow ◽  
Low Flow ◽  
Axial Flow Fan ◽  
Free Vortex ◽  
Flow Rates

Large axial flow fans are used in forced draft air cooled heat exchangers (ACHEs). Previous studies have shown that adverse operating conditions cause certain sectors of the fan, or the fan as a whole to operate at very low flow rates, thereby reducing the cooling effectiveness of the ACHE. The present study is directed towards the experimental and numerical analyses of the flow in the vicinity of an axial flow fan during low flow rates. This is done to obtain the global flow structure up and downstream of the fan. A near-free-vortex fan, designed for specific application in ACHEs, is used for the investigation. Experimental fan testing was conducted in a British Standard 848, type A fan test facility, to obtain the fan characteristic. Both steady-state and time-dependent numerical simulations were performed, depending on the operating condition of the fan, using the Realizable k-ε turbulence model. Good agreement is found between the numerically and experimentally obtained fan characteristic data. Using data from the numerical simulations, the time and circumferentially averaged flow field is presented. At the design flow rate the downstream fan jet mainly moves in the axial and tangential direction, as expected for a free-vortex design criteria, with a small amount of radial flow that can be observed. As the flow rate through the fan is decreased, it is evident that the down-stream fan jet gradually shifts more diagonally outwards, and the region where reverse flow occur between the fan jet and the fan rotational axis increases. At very low flow rates the flow close to the tip reverses through the fan, producing a small recirculation zone as well as swirl at certain locations upstream of the fan.

scholarly journals Stream recession curves and storage variability in small watersheds

2011 ◽  
Vol 8 (1) ◽  
pp. 1827-1860 ◽  
Author(s):  
N. Y. Krakauer ◽  
M. Temimi
Keyword(s):  
Land Surface ◽  
Gravity Data ◽  
Regional Scale ◽  
Water Storage ◽  
High Flow ◽  
Water Infiltration ◽  
Low Flow ◽  
Stepwise Multiple Regression ◽  
Flow Rates ◽  
Small Watersheds

Abstract. The pattern of streamflow recession after rain events offers clues about the relationship between watershed runoff (observable as river discharge) and water storage (not directly observable) and can help in water resource assessment and prediction. However, it has not been systematically analyzed across flow rates or related to independent assessments of terrestrial water storage. We characterized the streamflow recession pattern in 61 relatively undisturbed small watersheds (1–100 km2) across the coterminous United States with multiyear records of hourly streamflow from automated gauges. We used the North American Regional Reanalysis (NARR) to help identify periods where precipitation, snowmelt, and evaporation were small compared to streamflow. The order of magnitude of the recession timescale increases from 1 day at high flow rates (~1 mm/h) to 10 days at low flow rates (~0.01 mm/h), leveling off at low flow rates. There is significant variability in the recession timescale at a given flow rate between basins, correlated with climate and geomorphic variables such as the ratio of mean streamflow to precipitation and soil water infiltration capacity. Stepwise multiple regression was used to construct a six-variable predictive model that explained some 80% of the variance in recession timescale at high flow rates and 30–50% at low flow rates. Seasonal and interannual variability in storage shows similar time evolution to but is up to a factor of 10 smaller than regional-scale water storage variability estimated from GRACE satellite gravity data and from land surface modeling forced by observed meteorology. The discrepancy may point to a "disconnection" between the conceptual pool that supplies streamflow during dry periods and other dynamic pools such as soil moisture and deep groundwater.

Transport Phenomena in Novel Microstructures for Use in Thermal Separation Processes

2009 ◽  
Author(s):  
Lukas E. Wiesegger ◽  
Ralf P. Knauss ◽  
Thomas Winkler ◽  
Stefan Maikowske ◽  
Ju¨rgen J. Brandner ◽  
...  
Keyword(s):  
Gaseous Phase ◽  
Phase Distribution ◽  
Flow Direction ◽  
Test System ◽  
Operating Conditions ◽  
Low Flow ◽  
Optimum Operating ◽  
Liquid Distribution ◽  
Flow Rates ◽  
Vof Model

In the present work novel microstructures are developed and studied by means of analytical and numerical methods. These microstructures form part of a demonstration microdevice to carry out a distillation without chemical reaction, two for the liquid and gaseous phase distribution/collection, and one for the liquid/gas mass transfer (“μTU-I,-II”). A solution for the 3D velocity field for the velocity component w in flow direction can be given for the rectangular and semicircular microchannel by using a lubrication approximation which gives good realistic values for the Reynolds number at low flow rates comparing to the common 2D approaches. The μTU-I is studied using the CFD code (6.3 FLUENT ® - 3ddp) by the approach of the VOF model. The simulations are performed with the test system methanol/water (distillation). By varying the flow rates of both phases and the contact angle, the condition until the occurrence of flooding of the microunit “μTU-I” is determined. The flooding and the optimum operating conditions of a new optimized configuration “μTU-II” -microunit are also investigated by means of numerical simulation (CFD). It can be shown that longitudinal instability is generated. Based on several concepts for liquid and gaseous phase distribution/collection by the Forschungszentrum Karlsruhe, novel microstructures (“Liquid-Distribution-Collection-Microstructure”, “Vapor-Distribution-Collection-Microstructure”) for both phases are developed, studied and optimized by using CFD. The results of all studies are verified based on the demonstration device in the laboratory.

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