scholarly journals Antidepressant-induced differential ubiquitination of β-arrestins 1 and 2 in mononuclear leucocytes of patients with depression

2013 ◽  
Vol 16 (8) ◽  
pp. 1745-1754 ◽  
Author(s):  
Moran Golan ◽  
Gabriel Schreiber ◽  
Sofia Avissar
Keyword(s):  
Depressive Episode ◽  
Matched Control ◽  
Antidepressant Treatment ◽  
Receptor Desensitization ◽  
Age And Gender ◽  
Control Subjects ◽  
Functional Differences ◽  
Before And After ◽  
Mononuclear Leucocytes ◽  
And Gender

Abstract β-Arrestins 1 and 2, cytosolic proteins known to mediate receptor desensitization, endocytosis and G protein-independent signalling, are post-translationally modified by ubiquitination regulating their ability to serve as adaptors and scaffolds. β-Arrestins were suggested to play a role in the pathophysiology of depression and in antidepressant mechanism of action. To determine whether a depressive episode or antidepressant treatment induce significant selective differences in β-arrestin 1 and 2 levels or their ubiquitination patterns in leucocytes of patients with depression, 46 outpatients diagnosed with a depressive episode were examined before and after 4-wk antidepressant treatment compared with age- and gender-matched control subjects. β-Arrestin levels were measured by immunoblotting using anti-arrestin antibodies. Ubiquitination of β-arrestins was measured using anti-ubiquitin antibodies followed by an immunoprecipitation step and immunoblotting using anti-arrestin antibodies. Antidepressants induced selective alterations in leucocyte β-arrestin 1 and 2 levels and ubiquitination. The levels of β-arrestin 1 and 2 and their ubiquitinated forms in leucocytes of yet untreated patients with depression were significantly decreased in a symptom severity correlated manner compared to control subjects. Antidepressants normalized β-arrestin 1 and 2 levels and uncovered novel differences between the two isoforms: (a) while antidepressants normalized ubiquitination of β-arrestin 1, ubiquination of β-arrestin 2 was unaffected; (b) while under antidepressants ubiquitination extent of β-arrestin 1 positively correlated with its level, an inverse picture of negative correlation was found between ubiquitination extent of β-arrestin 2 and its level. We conclude that antidepressants may serve as a tool to detect functional differences between the two β-arrestin isoforms and that through these differential effects antidepressants can induce specific alterations in alternative cellular signalling.

Reducing Disruption of Circadian Temperature Rhythm Following Surgery

2001 ◽  
Vol 2 (4) ◽  
pp. 257-266 ◽  
Author(s):  
Lynne Farr ◽  
Catherine Todero ◽  
Lonna Boen
Keyword(s):  
Continuous Monitoring ◽  
Maxillofacial Surgery ◽  
Recovery Process ◽  
Age And Gender ◽  
Control Subjects ◽  
Temperature Rhythm ◽  
Before And After ◽  
Temperature Rhythms ◽  
And Gender ◽  
And Control

Temperature and other circadian rhythms are disrupted following surgery and other traumatic events. During recovery, coordination between temperature rhythms and other rhythmic physiologic processes is reduced. Studies of animals and humans have shown that return of synchrony is not immediate, but that it is important in the recovery process. The purpose of this study was to test a combination of cues that have been shown to adjust the timing of circadian temperature rhythm. The combined cues consisted of timed ingestion of caffeine and protein foods and adjustment of the sleep/wake cycle. The intervention was tested in 26 age-and gender-matched maxillofacial surgery patients. Patients were randomly assigned to control or experimental groups. Circadian temperature rhythm was measured by continuous monitoring with axillary probes and miniature recorders before and after surgery. Following surgery, both experimental and control subjects displayed 24-hour circadian temperature rhythms; however, the peak-to-trough difference was decreased more following surgery in the control subjects than in the subjects who had prepared for surgery by practicing the intervention. Control subjects also had less day-to-day stability in the phase of their rhythms following surgery. These results suggest that the intervention reduced circadian disruption following surgery and provides a way for patients to prepare themselves to resist rhythm changes.

scholarly journals Swallowing in Parkinson Patients versus Healthy Controls: Reliability of Measurements in Videofluoroscopy

2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Laura W. J. Baijens ◽  
Renée Speyer ◽  
Valéria Lima Passos ◽  
Walmari Pilz ◽  
Nel Roodenburg ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Interrater Reliability ◽  
Matched Control ◽  
Healthy Controls ◽  
Swallowing Function ◽  
Age And Gender ◽  
Control Subjects ◽  
Healthy Control ◽  
And Gender

Objective. To determine and describe the pathophysiological aspects of oropharyngeal swallowing in patients with Parkinson's disease more accurately, a pilot study of qualitative as well as quantitative parameters of swallowing was performed using videofluoroscopy (VFS).Methods. Ten patients with a diagnosis of idiopathic Parkinson's disease having dysphagic complaints and ten healthy age- and gender-matched control subjects underwent a standardized videofluoroscopic swallowing protocol. Information on the swallowing function was derived from temporal, spatial, and descriptive visuoperceptual parameters. Intra- and interrater reliability was calculated.Results. No significant differences were found between Parkinson patients and healthy control subjects for the majority of the reliable variables.Conclusions. It was concluded that swallowing function seemed to be preserved in the early stages of Parkinson's disease. Furthermore, the reliability of many quantitative as well as qualitative swallowing parameters proved insufficient, raising questions about the interpretation of study outcomes in videofluoroscopy.

Hair Magnesium content and its relation to Serum Magnesium level in children with Idiopathic Epilepsy

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Hamed Mahmoud Shatla ◽  
Mariam Fathy Abdel Maksoud ◽  
Raghda Mohamed Hesham Zaitoun ◽  
Alaa Rabie Abdel Baset Mahmoud
Keyword(s):  
Serum Magnesium ◽  
Serum Levels ◽  
Magnesium Content ◽  
Magnesium Level ◽  
Idiopathic Epilepsy ◽  
Age And Gender ◽  
Control Subjects ◽  
Significant Difference ◽  
And Gender ◽  
Patients With Epilepsy

Abstract Objective To measure the level of hair Mg, as well as its level in serum, in patients with epilepsy and compare them to the levels found in non-epileptic age and gender matched children, and to explore any potential correlation between either serum or hair level of magnesium and seizure characteristics in children with idiopathic epilepsy. Methods An observational cross-sectional study including 50 children with idiopathic epilepsy and 100 non-epileptic age and gender matched control subjects. Cases were subjected to full history taking, examination and measurements of serum and hair levels of magnesium, control subjects only had their serum and hair level of magnesium measured as for the cases. Results The mean serum magnesium was 29.11 ± 13.42 ug/ml for cases and 27.67 ± 7.24 ug/ml for controls and the median hair level of magnesium was 42.22 ug/g with IQR of 25.9 - 56.82 for cases and 38.6 ug/g with IQR of 25.21 - 61.25 for controls. No statistically significant difference was observed between both groups as regards either serum or hair magnesium levels. No statistically significant correlation was observed between either hair or serum levels of magnesium and seizure characteristics though the correlations were nearing statistical significance for the hair magnesium content. Conclusion Hair magnesium level may be better correlated to seizure characteristics and control than serum levels in patients with epilepsy.

Influence of Age and Gender Before and After Liver Transplantation

2013 ◽  
Vol 19 (2) ◽  
pp. 122-134 ◽  
Author(s):  
Patrizia Burra ◽  
Eleonora De Martin ◽  
Stefano Gitto ◽  
Erica Villa
Keyword(s):  
Liver Transplantation ◽  
Age And Gender ◽  
Before And After ◽  
And Gender

Increased Documentation and Management of Pediatric Obesity Following Implementation of an EMR Upgrade and Education

2011 ◽  
Vol 51 (1) ◽  
pp. 31-38 ◽  
Author(s):  
Jennifer Keehbauch ◽  
Gretchen San Miguel ◽  
Leslie Drapiza ◽  
Julie Pepe ◽  
Richard Bogue ◽  
...  
Keyword(s):  
Clinical Management ◽  
Rate Of Change ◽  
Physician Education ◽  
Overweight Children ◽  
Age And Gender ◽  
Training And Education ◽  
Medicine Resident ◽  
Before And After ◽  
And Gender ◽  
One Year

Background: Overweight children are often not identified or counseled. Purpose: We assessed the documentation rate and clinical management of overweight children before and after an electronic medical record (EMR) upgrade calculating body mass index (BMI) percentile for age and gender. Methods: Family Medicine resident and faculty physicians at two sites received an EMR upgrade; Site1 physicians also received BMI training and education. From two years before to one year after the upgrade, randomly selected charts were reviewed for all encounters with overweight children for documentation of obesity and clinical management. Results: After the EMR upgrade, documentation and counseling rates significantly improved at both sites but the rate of change was greater for Site 1; postintervention documentation was significantly greater for Site 1 vs. Site 2 (40% vs. 28%, P<0.01). Conclusions: We found an increase in documentation and management of overweight children following an EMR upgrade that calculates BMI percentiles for age and gender. Physician education was an important adjunct.

scholarly journals Direct causes of death in Japanese patients with hypopituitarism as analyzed from a nation-wide autopsy database

2004 ◽  
pp. 149-152 ◽  
Author(s):  
H Kaji ◽  
K Chihara
Keyword(s):  
Relative Frequency ◽  
Causes Of Death ◽  
Matched Control ◽  
Heart Diseases ◽  
Japanese Patients ◽  
Control Population ◽  
Term Care ◽  
Age And Gender ◽  
And Gender ◽  
Matched Control Population

OBJECTIVES: The direct causes of death in Japanese patients with hypopituitarism remain unclear. In this study, the direct causes of death were investigated and compared between Japanese patients with hypopituitarism from a nation-wide autopsy database and an age- and gender-matched control population from national reports. SUBJECTS: Three hundred and ninety-one subjects with hypopituitarism who had died were selected from a nation-wide autopsy database (1984-1993). The ratios of each cause of death among the age- and gender-matched control population were derived from national reports. RESULTS: In subjects with hypopituitarism, an increased relative frequency of death from cerebrovascular diseases (male; 2.02 (95% confidence interval (CI) 1.45-2.82), female; 1.73 (95% CI 1.18-2.52)) was found. In particular, the relative frequency of death from cerebral hemorrhage was 4.60 (95% CI 2.95-7.17) in male and 4.80 (95% CI 2.90-7.94) in female subjects with hypopituitarism. Unexpectedly, a decreased relative frequency of death from all heart diseases (male; 0.439 (95% CI 0.277-0.696), female; 0.267 (95% CI 0.149-0.478)) was found in subjects with hypopituitarism, although there was no difference between subjects with hypopituitarism and controls in the frequency of death from ischemic heart disease. CONCLUSIONS: These results provide useful information for the long-term care of Japanese patients with hypopituitarism.

scholarly journals Pengaruh Terapi Kompres Air Hangat terhadap Nyeri Post Operasi ORIF pada Pasen Fraktur di RSUD Dr. Doris Sylvanus Palangka Raya

2021 ◽  
Vol 6 (2) ◽  
pp. 82-87
Author(s):  
Vina Agustina ◽  
Suryagustina Suryagustina ◽  
Henry Wiyono
Keyword(s):  
Postoperative Pain ◽  
Health Workers ◽  
Wilcoxon Test ◽  
Warm Water ◽  
P Value ◽  
Internal Factors ◽  
Age And Gender ◽  
Common Complaint ◽  
Before And After ◽  
And Gender

A fracture or often called a fracture is a break in the continuity of bone tissue and is determined according to its type and extent. Pain is the most common complaint in fracture patients. Based on the results of the preliminary survey on February 14, 2018, in the Dahlia room, RSUD dr. Doris Sylvanus Palangka Raya, from 10 patients, it was found that 6 patients did not know that warm water compress therapy could reduce pain and 4 patients had heard that warm water compresses could reduce pain but still did not dare to take action alone without advice from a health professional. The results of the analysis research with the Wilcoxon test obtained a significant p-value (0.008 <0.05), then Ha is accepted, which means that there is an effect of giving warm water compress therapy before and after on the postoperative pain of ORIF in fracture patients. It can be concluded that the factors that influence the provision of warm water compress therapy to postoperative pain in ORIF fracture patients are age and gender (internal factors) and external factors including the compressing medium, warm water temperature, and the duration of the compress so that the warm water compress method is effective against decreasing intensity. pain. Health workers are advised to apply warm compress therapy as an intervention to reduce postoperative pain in patients with ORIF fractures.

scholarly journals Implantation of Cardioverter-Defibrillator in Children With Long-QT Syndrome: Assessment of Indications, Efficacy, and Safety Based on 10-Year Experience

Kardiologiia ◽  
2018 ◽  
Vol 58 (12) ◽  
pp. 52-58 ◽  
Author(s):  
R. A. Ildarova ◽  
M. A. Shkolnikova ◽  
S. A. Termosesov
Keyword(s):  
Sudden Cardiac Arrest ◽  
Efficacy And Safety ◽  
Primary And Secondary Prevention ◽  
Icd Implantation ◽  
Age And Gender ◽  
Before And After ◽  
History Of ◽  
The Mean ◽  
High Doses ◽  
And Gender

Purpose: to assess specificities of course of the long­QT syndrome in children before and after implantation of cardioverter­defibrillator (ICD), and optimization of indications to ICD­therapy.Materials and methods. We included in this study 48 children with long­QT syndrome from 44 unrelated families (28 boys and 20 girls), who underwent ICD implantation at the mean age 11.8±3.8 years. Mean duration of follow­up after implantation was 5.2±2.8 years. Data from these children were compared with those from 59 children of comparable age and gender with long­QT syndrome from 46 unrelated families receiving antiarrhythmic therapy (β­adrenoblockers). We assessed clinical and electrocardiographic characteristics of the disease obtained at initial visit and their dynamics thereafter.Results. Children with long­QT syndrome and ICD were mainly probands with interval QT longer than 500 ms, recurrent syncope and often history of sudden cardiac arrest requiring high doses of β­adrenoblockers for control of ventricular tachyarrhythmias.Conclusion. ICD implantation is an effective and safe method both of primary and secondary prevention of sudden cardiac death in children with long­QT syndrome.

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