Using the Strategy of Inducing and Genetically Transforming Plant Suspension Cells to Produce High Value-Added Bioactive Substances

Improvement of the Quality of Ginkgo biloba Leaves Fermented by Eurotium cristatum as High Value-Added Feed

Processes ◽

10.3390/pr7090627 ◽

2019 ◽

Vol 7 (9) ◽

pp. 627

Author(s):

Minmin Zou ◽

Xiaohan Guo ◽

Yan Huang ◽

Fuliang Cao ◽

Erzheng Su ◽

...

Keyword(s):

Ginkgo Biloba ◽

Value Added ◽

Inoculum Size ◽

Bioactive Substances ◽

Fermentation Time ◽

Nutrient Changes ◽

Unpleasant Smell ◽

Optimal Medium ◽

Ginkgolic Acids

Ginkgo biloba leaves are well known for their high content of nutrients and bioactive substances. However, unpleasant smell and a small number of ginkgolic acids greatly reduce the utilization of the leaves. In this work, solid-state fermentation of G. biloba leaves using Eurotium cristatum was studied by investigation of the nutrient changes and its feasibility as a functional feed. E. cristatum could grow on pure G. biloba leaves and the addition of excipients could significantly improve the growth of E. cristatum. The optimal medium was with 10% (w/w) of whole G. biloba seeds and the optimized water content, pH, inoculum size and fermentation time were 45% (w/w), 4.5, 4.76 × 107 CFU/100 g wet medium, and eight days, respectively. Under the optimal conditions, the spore number increased by about 40 times. The content of flavonoids was greatly increased by 118.6%, and the protein and polyprenyl acetates (PPAs) were increased by 64.9% and 10.6%, respectively. The ginkgolic acids, lignin, and cellulose were decreased by 52.4%, 38.5%, and 20.1% than before, respectively. Furthermore, the fermented G. biloba leaves showed higher antioxidant activity and held more aroma substances. Thus, G. biloba leaves fermented by E. cristatum have potential as s high value-added feed. This is the first investigation of E. cristatum fermentation on ginkgo leaves, which will facilitate the use of ginkgo leaves in the feed industry.

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Biofilm Eradication Using Biogenic Silver Nanoparticles

Molecules ◽

10.3390/molecules25092023 ◽

2020 ◽

Vol 25 (9) ◽

pp. 2023 ◽

Cited By ~ 5

Author(s):

María Belén Estevez ◽

Sofía Raffaelli ◽

Scott G. Mitchell ◽

Ricardo Faccio ◽

Silvana Alborés

Keyword(s):

Silver Nanoparticles ◽

Colloidal Stability ◽

Fatty Acid Content ◽

Membrane Integrity ◽

Antimicrobial Properties ◽

Value Added ◽

Industrial Applications ◽

Bioactive Substances ◽

Cell Membrane Integrity ◽

E Coli

Microorganisms offer an alternative green and scalable technology for the synthesis of value added products. Fungi secrete high quantities of bioactive substances, which play dual-functional roles as both reducing and stabilizing agents in the synthesis of colloidal metal nanoparticles such as silver nanoparticles, which display potent antimicrobial properties that can be harnessed for a number of industrial applications. The aim of this work was the production of silver nanoparticles using the extracellular cell free extracts of Phanerochaete chrysosporium, and to evaluate their activity as antimicrobial and antibiofilm agents. The 45–nm diameter silver nanoparticles synthesized using this methodology possessed a high negative surface charge close to −30 mV and showed colloidal stability from pH 3–9 and under conditions of high ionic strength ([NaCl] = 10–500 mM). A combination of environmental SEM, TEM, and confocal Raman microscopy was used to study the nanoparticle-E. coli interactions to gain a first insight into their antimicrobial mechanisms. Raman data demonstrate a significant decrease in the fatty acid content of E. coli cells, which suggests a loss of the cell membrane integrity after exposure to the PchNPs, which is also commensurate with ESEM and TEM images. Additionally, these biogenic PchNPs displayed biofilm disruption activity for the eradication of E. coli and C. albicans biofilms.

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Sustainable 2,5-furandicarboxylic synthesis by a direct 5-hydroxymethylfurfural fuel cell based on a bifunctional PtNiSx catalyst

Chemical Communications ◽

10.1039/d0cc06087a ◽

2020 ◽

Vol 56 (88) ◽

pp. 13611-13614

Author(s):

Jialu Wang ◽

Xian Zhang ◽

Guozhong Wang ◽

Yunxia Zhang ◽

Haimin Zhang

Keyword(s):

Fuel Cell ◽

Electric Energy ◽

Value Added ◽

Chemical Energy ◽

New Type

A new type of direct 5-hydroxymethylfurfural (HMF) oxidation fuel cell based on a bifunctional PtNiSx/CB catalyst not only transformed chemical energy into electric energy but also converted HMF into value-added 2,5-furandicarboxylic (FDCA).

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