Phenylalanine Interacts with Oleic Acid-Based Vesicle Membrane. Understanding the Molecular Role of Fibril–Vesicle Interaction under the Context of Phenylketonuria

Soybean is the second largest source of oil worldwide. Developing soybean varieties with high levels of oleic acid is a primary goal of the soybean breeders and industry. Edible oils containing high level of oleic acid and low level of linoleic acid are considered with higher oxidative stability and can be used as a natural antioxidant in food stability. All developed high oleic acid soybeans carry two alleles; GmFAD2-1A and GmFAD2-1B. However, when planted in cold soil, a possible reduction in seed germination was reported when high seed oleic acid derived from GmFAD2-1 alleles were used. Besides the soybean fatty acid desaturase (GmFAD2-1) subfamily, the GmFAD2-2 subfamily is composed of five members, including GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E. Segmental duplication of GmFAD2-1A/GmFAD2-1B, GmFAD2-2A/GmFAD2-2C, GmFAD2-2A/GmFAD2-2D, and GmFAD2-2D/GmFAD2-2C have occurred about 10.65, 27.04, 100.81, and 106.55 Mya, respectively. Using TILLING-by-Sequencing+ technology, we successfully identified 12, 8, 10, 9, and 19 EMS mutants at the GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E genes, respectively. Functional analyses of newly identified mutants revealed unprecedented role of the five GmFAD2-2A, GmFAD2-2B, GmFAD2-2C, GmFAD2-2D, and GmFAD2-2E members in controlling the seed oleic acid content. Most importantly, unlike GmFAD2-1 members, subcellular localization revealed that members of the GmFAD2-2 subfamily showed a cytoplasmic localization, which may suggest the presence of an alternative fatty acid desaturase pathway in soybean for converting oleic acid content without substantially altering the traditional plastidial/ER fatty acid production.

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Evidence against local neural mechanism for intestinal postprandial hyperemia

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1983.245.3.h437 ◽

1983 ◽

Vol 245 (3) ◽

pp. H437-H446 ◽

Cited By ~ 2

Author(s):

R. A. Nyhof ◽

C. C. Chou

Keyword(s):

Oxygen Consumption ◽

Oleic Acid ◽

Neural Mechanism ◽

Jejunal Mucosa ◽

Vasoactive Substances ◽

Before And After ◽

Anesthetized Dogs ◽

Postprandial Hyperemia

The role of local intestinal nerves in the nutrient-induced intestinal hyperemia was investigated in jejunal segments of anesthetized dogs by comparing the hyperemic effect of intraluminal glucose and oleic acid solutions before and after mucosal anesthesia and infusions of methysergide, hexamethonium, and tetrodotoxin. Methysergide, hexamethonium, and tetrodotoxin all failed to alter either the vascular or metabolic responses to luminal placement of glucose or oleic acid. The increases in blood flow and oxygen uptake produced by glucose or oleic acid, however, were blocked or attenuated after exposing the mucosa to dibucaine. The effect was norepinephrine due to an altered vascular response to vasoactive substances as dibucaine did not alter vascular responses to isoproterenol or norepinephrine. Dibucaine, however, inhibited active transport and increased passive transport of glucose across rat intestinal sacs in vitro. Oxygen consumption of the canine jejunal mucosa was also inhibited by dibucaine in vitro. It seems that inhibition of the nutrient-induced intestinal hyperemia by dibucaine is due, at least in part, to its effect on oxygen consumption and glucose transport of the mucosal epithelial cells. Nutrient-induced hyperemia appears not to be neurally mediated but more closely related to metabolism.

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Role of the PLA2-Activated Neutrophilic Oxidative Stress in Oleic Acid-Induced Acute Lung Injury

Tuberculosis and Respiratory Diseases ◽

10.4046/trd.2010.68.2.55 ◽

2010 ◽

Vol 68 (2) ◽

pp. 55 ◽

Cited By ~ 2

Author(s):

Young Man Lee ◽

Byung Yong Kim ◽

Yoon Yub Park

Keyword(s):

Oxidative Stress ◽

Acute Lung Injury ◽

Oleic Acid ◽

Lung Injury

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The role of cytochrome b5 in Δ12 desaturation of oleic acid by microsomes of safflower (Carthamus tinctorius L.)

Archives of Biochemistry and Biophysics ◽

10.1016/0003-9861(91)90319-e ◽

1991 ◽

Vol 284 (2) ◽

pp. 431-436 ◽

Cited By ~ 48

Author(s):

Ellen V. Kearns ◽

Suzanne Hugly ◽

Chris R. Somerville

Keyword(s):

Oleic Acid ◽

Cytochrome B5 ◽

Carthamus Tinctorius

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Lipids Affect the Cryptococcus neoformans-Macrophage Interaction and Promote Nonlytic Exocytosis

Infection and Immunity ◽

10.1128/iai.00564-17 ◽

2017 ◽

Vol 85 (12) ◽

Cited By ~ 6

Author(s):

Sabrina J. Nolan ◽

Man Shun Fu ◽

Isabelle Coppens ◽

Arturo Casadevall

Keyword(s):

Oleic Acid ◽

Cryptococcus Neoformans ◽

Lipid Droplets ◽

Fungal Pathogens ◽

Cellular Lipid ◽

Content Type ◽

Acid Supplementation

ABSTRACT Many microbes exploit host cellular lipid droplets during the host-microbe interaction, but this phenomenon has not been extensively studied for fungal pathogens. In this study, we analyzed the role of lipid droplets during the interaction of Cryptococcus neoformans with macrophages in the presence and the absence of exogenous lipids, in particular, oleate. The addition of oleic acid increased the frequency of lipid droplets in both C. neoformans and macrophages. C. neoformans responded to oleic acid supplementation by faster growth inside and outside macrophages. Fungal cells were able to harvest lipids from macrophage lipid droplets. Supplementation of C. neoformans and macrophages with oleic acid significantly increased the rate of nonlytic exocytosis while having no effect on lytic exocytosis. The process for lipid modulation of nonlytic exocytosis was associated with actin changes in macrophages. In summary, C. neoformans harvests lipids from macrophages, and the C. neoformans-macrophage interaction is modulated by exogenous lipids, providing a new tool for studying nonlytic exocytosis.

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Role of Oleic Acid in the Gut-Liver Axis: From Diet to the Regulation of Its Synthesis via Stearoyl-CoA Desaturase 1 (SCD1)

Nutrients ◽

10.3390/nu11102283 ◽

2019 ◽

Vol 11 (10) ◽

pp. 2283 ◽

Cited By ~ 11

Author(s):

Elena Piccinin ◽

Marica Cariello ◽

Stefania De Santis ◽

Simon Ducheix ◽

Carlo Sabbà ◽

...

Keyword(s):

Oleic Acid ◽

Fatty Acid ◽

Olive Oil ◽

Intestinal Inflammation ◽

Principal Component ◽

Monounsaturated Fatty Acids ◽

Beneficial Effects ◽

Stearoyl Coa Desaturase ◽

The Impact

The consumption of an olive oil rich diet has been associated with the diminished incidence of cardiovascular disease and cancer. Several studies have attributed these beneficial effects to oleic acid (C18 n-9), the predominant fatty acid principal component of olive oil. Oleic acid is not an essential fatty acid since it can be endogenously synthesized in humans. Stearoyl-CoA desaturase 1 (SCD1) is the enzyme responsible for oleic acid production and, more generally, for the synthesis of monounsaturated fatty acids (MUFA). The saturated to monounsaturated fatty acid ratio affects the regulation of cell growth and differentiation, and alteration in this ratio has been implicated in a variety of diseases, such as liver dysfunction and intestinal inflammation. In this review, we discuss our current understanding of the impact of gene-nutrient interactions in liver and gut diseases, by taking advantage of the role of SCD1 and its product oleic acid in the modulation of different hepatic and intestinal metabolic pathways.

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The Effect of Electric Charges Formed during Repeated Deformations on the Fatigue Resistance of Vulcanizates

Rubber Chemistry and Technology ◽

10.5254/1.3542237 ◽

1960 ◽

Vol 33 (4) ◽

pp. 970-970 ◽

Cited By ~ 2

Author(s):

B. A. Dogadkin ◽

V. E. Gul ◽

N. A. Morozova

Keyword(s):

Zinc Oxide ◽

Oleic Acid ◽

Fatigue Resistance ◽

Acetylene Black ◽

Black Oil

Abstract Electroelastic and triboelastic effects result in the formation of electric charges on articles made from polymers undergoing deformation. It was of interest to determine the effect of the charges formed during deformation on the fatigue resistance of vulcanizates. No convincing experiments clearly demonstrating the role of electric charges arising during friction or deformation had been carried out previously. Vulcanizates containing different amounts of acetylene black were tested in a machine in which cylindrical specimens with thickened ends were subjected to repeated bending at 1300 cycles/minute. The stocks had the following composition (in parts by weight) : SKS-30A 100, sulfur 2, technical stearin 2, Rubrax 5, black oil 4, MBTS 0.6, diphenylguanidine 0.8, zinc oxide 5, oleic acid 1. Increasing amounts of acetylene black were added to this composition: 6, 12, 18, 20, 22, 30, 40 and 75 wt. parts per 100 wt. parts of rubber.

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