Construction of Ultralarge Two-Dimensional Fluorescent Protein Arrays via a Reengineered Rhodamine B-Based Molecular Tool

During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited. Recently, we reported that translational enhancement by a Dictyostelium gene sequence (TED) boosted protein expression even without an expression inducer in Escherichia coli. Here, we demonstrate a generally applicable molecular tool using the expression of green fluorescent protein enhanced by TED. By inserting a module related to TED into the cloning site in advance, we effectively screened E. coli colonies harboring the desired plasmid functions in a prokaryote ( Magnetospirillum gryphiswaldense) or eukaryote ( Dictyostelium discoideum). Thus, our system represents a user-friendly technique for cloning.

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Assessment of biological characteristics of adipose tissue-derived stem cells co-labeled with Molday ION Rhodamine B™ and green fluorescent protein in vitro

Molecular Medicine Reports ◽

10.3892/mmr.2013.1694 ◽

2013 ◽

Vol 8 (5) ◽

pp. 1446-1452 ◽

Cited By ~ 2

Author(s):

HUA NAN ◽

JIACHENG HUANG ◽

HONGMIAN LI ◽

QIONG LI ◽

DALIE LIU

Keyword(s):

Stem Cells ◽

Adipose Tissue ◽

Green Fluorescent Protein ◽

Rhodamine B ◽

Fluorescent Protein ◽

Biological Characteristics ◽

Green Fluorescent

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The synthesis and the photocatalytic degradation property of the nano-MoS2

Functional Materials Letters ◽

10.1142/s179360471650065x ◽

2016 ◽

Vol 09 (05) ◽

pp. 1650065 ◽

Cited By ~ 5

Author(s):

Xiaoqin Man ◽

Lixin Yu ◽

Jiaju Sun ◽

Songchu Li

Keyword(s):

Optical Properties ◽

Photocatalytic Degradation ◽

Rhodamine B ◽

Potential Application ◽

Ph Value ◽

Blue Emission ◽

Precursor Solution ◽

Two Dimensional ◽

Laminated Structure ◽

Ultraviolet Range

Recently, the two-dimensional molybdenum disulfide (MoS2) materials have attracted much interest owing to its potential application in optoelectronics and catalyze. In this paper, nano-MoS2was prepared via a hydrothermal method through changing the pH value of the precursor solution. The structure and optical properties are systematically studied. The results indicate that laminated MoS2structure is obtained. The pH value of precursor solution affects the laminated structure. The strong absorption in ultraviolet range and blue emission are observed in the prepared MoS2structures. The photocatalytic properties of MoS2by degrading Rhodamine B under ultraviolet light were observed.

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Point Mutagenesis and Cocrystallization of Wild-Type and Mutant Proteins: A Study of Solid-Phase Coexistence in Two-Dimensional Protein Arrays

Langmuir ◽

10.1021/la010227n ◽

2001 ◽

Vol 17 (19) ◽

pp. 5731-5735 ◽

Cited By ~ 1

Author(s):

Sammy J. Farah ◽

Szu-Wen Wang ◽

Wei-Hau Chang ◽

Channing R. Robertson ◽

Alice P. Gast

Keyword(s):

Solid Phase ◽

Phase Coexistence ◽

Two Dimensional ◽

Wild Type ◽

Protein Arrays ◽

Mutant Proteins ◽

Point Mutagenesis

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Designing Two-Dimensional Protein Arrays through Fusion of Multimers and Interface Mutations

Nano Letters ◽

10.1021/acs.nanolett.5b01499 ◽

2015 ◽

Vol 15 (8) ◽

pp. 5235-5239 ◽

Cited By ~ 27

Author(s):

James F. Matthaei ◽

Frank DiMaio ◽

Jeffrey J. Richards ◽

Lilo D. Pozzo ◽

David Baker ◽

...

Keyword(s):

Two Dimensional ◽

Protein Arrays

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Supramolecular Reinforcement of a Large Pore 2D Covalent Organic Framework

10.26434/chemrxiv-2021-89q05 ◽

2021 ◽

Author(s):

Shashini Diwakara ◽

Whitney Ong ◽

Yalini Wijesundara ◽

Robert Gearhart ◽

Fabian Herbert ◽

...

Keyword(s):

Pore Size ◽

Fluorescent Protein ◽

Fluorescent Proteins ◽

Nitrogen Adsorption ◽

Two Dimensional ◽

X Ray Diffraction ◽

Structural Reinforcement ◽

Transmission Electron ◽

Covalently Linked ◽

Green Fluorescent

Two dimensional covalent organic frameworks (2D-COFs) are a class of crystalline porous organic polymers that consist of covalently linked, two dimensional sheets that can stack together through non-covalent interactions. Here we report the synthesis of a novel COF, called PyCOFamide, which has an experimentally observed pore size that is greater than 6 nm in diameter. This is among the largest pore size reported to date for a 2D-COF. PyCOFamide exhibits permanent porosity and high crystallinity as evidenced by the nitrogen adsorption, powder X-ray diffraction, and high-resolution transmission electron microscopy. We show that the pore size of PyCOFamide is large enough to accommodate fluorescent proteins such as Superfolder green fluorescent protein and mNeonGreen. This work demonstrates the utility of non-covalent structural reinforcement in 2D-COFs to produce larger, persistent pore sizes than previously possible.

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Enhanced Photocatalytic Activity of Two Dimensional SrTiO3 Nano Structures for Dye Degradation

10.21203/rs.3.rs-818506/v1 ◽

2021 ◽

Author(s):

Y. AlIssa ◽

S. T. Sajjadian ◽

S. M. Hamidi ◽

S. Sadeghi ◽

M. R. Nabid

Keyword(s):

Photocatalytic Activity ◽

Rhodamine B ◽

Dye Degradation ◽

Semiconductor Material ◽

Two Dimensional ◽

Plasmonic Material ◽

Nano Structures ◽

Rhodamine B Dye ◽

Degradation Ability ◽

Made In

Abstract The combination of the properties of nanostructures, photocatalytic semiconductors, and plasmonic materials can significantly contribute to improving the performance of the entire photocatalysis process. We tried to produce a unique catalyst by combining these three properties. We have produced a two-dimensional nanostructure which was then coated with gold as a plasmonic material followed by SrTiO3 as semiconductor material. The result was the production of the first catalyst of its kind made in this way, which offered notable results. The degradation ability of the sample was examined with different degrees of pH of rhodamine B dye, where we achieved the highest degradation, 16%, at pH10.

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