Ion Chromatography with Pulsed Amperometric Detection

A practical method for the determination of cyanide in bamboo shoots has been developed using microdiffusion preparation integrated with ion chromatography–pulsed amperometric detection (IC-PAD). Cyanide was released from bamboo shoots after Conway cell microdiffusion, and then analysed by IC-PAD. In comparison with the previously reported methods, derivatization and ion-pairing agent addition were not required in this proposed microdiffusion combined with IC-PAD method. The microdiffusion parameters were optimized including hydrolysis systems, temperature, time, and so on. Under the optimum conditions, the linear range of the calibration curve for cyanide was 0.2–200.0 µg kg −1 with satisfactory correlation coefficients of 0.9996 and the limit of detection was 0.2 µg kg −1 ( S/N = 3). The spiked recovery range was from 92.8 to 98.6%. The intra-day and inter-day relative standard deviations of cyanide were 2.7–14.9% and 3.0–18.3%, respectively. This method was proved to be convenient in operation with high sensitivity, precision and accuracy, and was successfully applied in the determination of cyanide in bamboo shoot samples.

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The Ion Chromatography Intergrated Amperometry Method to Detectsulfonamides

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.726-731.1544 ◽

2013 ◽

Vol 726-731 ◽

pp. 1544-1547

Author(s):

Wei De Lu ◽

Zhan Qing Ma ◽

Jun Feng Rao

Keyword(s):

Ion Chromatography ◽

High Performance ◽

Exchange Resin ◽

Reference Electrode ◽

Amperometric Detection ◽

Solution Concentration ◽

Pulsed Amperometric Detection ◽

Naoh Solution ◽

Food And Feed ◽

First Time

In this study, the high performance ion chromatography integrated pulsed amperometric detection (HPIC-IPAD) was appliedfor the first time to detect 10 sulfonamides. In this experiment,we used Dionex AS18 (250mm×2mm) and AG18 (50mm×2mm) columnsfilled with an anion exchange resin, with 14 mmol / L NaOH solution concentration. The Analysis temperature was 30 ° C, the flow rate was 0.25 mL / min, and the injection volume was 10 μL. The detection was through pulsed amperometric detection cell with Au electrode and reference electrode pH-Ag/AgCl as working electrodes. Take the standard stock solution compounding 0.06,0.15,0.30,1.5,3.0 mg / L standard solutions respectively,build linear regression on chromatographic peak area as Y and analyte mass concentration of as X (mg / L). In the range of 0.06-3.0 mg / L, the 10 sulfonamides response appeared a linear relationship (R> 0.9989). This method can be considered for the detection of trace sulfonamides substances in cosmetics, food, and feed.

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Development of an Analytical Protocol for Determination of Cyanide in Human Biological Samples Based on Application of Ion Chromatography with Pulsed Amperometric Detection

Journal of Analytical Methods in Chemistry ◽

10.1155/2017/7157953 ◽

2017 ◽

Vol 2017 ◽

pp. 1-7 ◽

Cited By ~ 6

Author(s):

Ewa Jaszczak ◽

Marek Ruman ◽

Sylwia Narkowicz ◽

Jacek Namieśnik ◽

Żaneta Polkowska

Keyword(s):

Ion Chromatography ◽

Biological Samples ◽

Amperometric Detection ◽

Sample Pretreatment ◽

Cyanide Ion ◽

Pulsed Amperometric Detection ◽

Analytical Protocol ◽

Good Repeatability ◽

Optimized Conditions

A simple and accurate ion chromatography (IC) method with pulsed amperometric detection (PAD) was proposed for the determination of cyanide ion in urine, sweat, and saliva samples. The sample pretreatment relies on alkaline digestion and application of Dionex OnGuard II H cartridge. Under the optimized conditions, the method showed good linearity in the range of 1–100 μg/L for urine, 5–100 μg/L for saliva, and 3–100 μg/L for sweat samples with determination coefficients (R)>0.992. Low detection limits (LODs) in the range of 1.8 μg/L, 5.1 μg/L, and 5.8 μg/L for urine, saliva, and sweat samples, respectively, and good repeatability (CV < 3%, n=3) were obtained. The proposed method has been successfully applied to the analysis of human biological samples.

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