Characterization of the cDNA encoding human nucleophosmin and studies of its role in normal and abnormal growth

Biochemistry ◽  
1989 ◽  
Vol 28 (3) ◽  
pp. 1033-1039 ◽  
Author(s):  
Wai Yee Chan ◽  
Qing Rong Liu ◽  
Jimo Borjigin ◽  
Harris Busch ◽  
Owen M. Rennert ◽  
...  
Keyword(s):  
HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 519d-519
Author(s):  
A. Otero ◽  
R. Perry ◽  
F. Ewers ◽  
R.T. Fernandez

A swelling of the rootstock shank, described as Root Mass Proliferation, has been frequently found in the field on apple trees of Mark rootstock. Swelling usually first appears on trees after they have been established for more than 3 years. The abnormal growth occurs above the soil line on the exposed rootstock shank and it extends to a depth of 10 - 15 cm below soil. Anatomical studies were conducted on maiden nursery trees and trees having been in the orchard for 3 to 6 years with light microscopy. In older trees, changes in normal tissue development occurred in the 2-4 cm outer zone of the swelling surface. Changing direction and proportion of xylem components gives an appearance of tracheiry elements developing in a circular pattern. Abnormal xylem parenchyma seems to have its origin at the xylem parenchyma rays, which follow a straight plain of cell division. Clusters of lignified root initiation points are often found in the outer part of xylem, all around the rootstock shank. Removal of bark and phloem exposes hard nodules, which were found to consist of tracheiry elements surrounded by lignified parenchyma cells all between xylem and phloem tissue encircling the rootstock shank. Abnormal development of xylem vessels suggests that there is an anatomical association between water transport and a reported physiological drought sensitivity of trees on this rootstock.


2012 ◽  
Vol 12 (3) ◽  
pp. 99-107 ◽  
Author(s):  
Victor Satoru Saito ◽  
Maria Virginia Urso-Guimarães

This is the first study about galls, gall makers and associated fauna of the Ecological Station of Jataí. Galls are plant structures formed by abnormal growth of cells, tissues or organs induced by several organisms, as fungous, nematoids and insects. Five areas of the conservation unity, two in the phytophysionomy of cerrado in regeneration and three in the cerradão area were studied, totalizing 69 morphotypes of galls on 41 host species from 24 families. This is the first record of Annonaceae as the richest family in morphotypes in Brazil; 34 gall makers and associated fauna were identified, which 23 Diptera (67.4%), eight Hymenoptera (23.5%), two Hemiptera (5.8%) and one Thysanoptera (2.9%). Were described 41 new morphotypes of gall and made the first characterization of gall on Maprounea guianensis Aubl., Acosmium subelegans (Mohlenbr.) Yakovlev., Strychnosbicolor Progel, Eriotheca gracilipes K. Schum., Stryphnodendron obovatum Benth., Broyesum gaudichaudii Trécul, Psychotria suterella Müll. Arg., Psychotria trichophora Müll. Arg. and Serjania erecta Radlk.


Genetics ◽  
1999 ◽  
Vol 153 (4) ◽  
pp. 1629-1640 ◽  
Author(s):  
Virginie Coustou ◽  
Carole Deleu ◽  
Sven J Saupe ◽  
Joël Bégueret

Abstract The het-s locus is one of nine known het (heterokaryon incompatibility) loci of the fungus Podospora anserina. This locus exists as two wild-type alleles, het-s and het-S, which encode 289 amino acid proteins differing at 13 amino acid positions. The het-s and het-S alleles are incompatible as their coexpression in the same cytoplasm causes a characteristic cell death reaction. We have proposed that the HET-s protein is a prion analog. Strains of the het-s genotype exist in two phenotypic states, the neutral [Het-s*] and the active [Het-s] phenotype. The [Het-s] phenotype is infectious and is transmitted to [Het-s*] strains through cytoplasmic contact. het-s and het-S were associated in a single haploid nucleus to generate a self-incompatible strain that displays a restricted and abnormal growth. In the present article we report the molecular characterization of a collection of mutants that restore the ability of this self-incompatible strain to grow. We also describe the functional analysis of a series of deletion constructs and site-directed mutants. Together, these analyses define positions critical for reactivity and allele specificity. We show that a 112-amino-acid-long N-terminal peptide of HET-s retains [Het-s] activity. Moreover, expression of a mutant het-s allele truncated at position 26 is sufficient to allow propagation of the [Het-s] prion analog.


1994 ◽  
Vol 338 ◽  
Author(s):  
E. M. Zielinski ◽  
R. P. Vinci ◽  
J. C. Bravman

ABSTRACTGrain size distribution and texture of thin films of sputtered copper were characterized as a function of underlayer choice, deposition temperature and annealing temperature. For Cu deposited on Ta at room temperature and annealed at 100, 150, 200 and 250 °C, abnormal growth of the (100) orientation was observed for annealing temperatures above 150 °C, resulting in a bimodal grain size distribution. This orientation was also seen to grow abnormally for films deposited and annealed under identical conditions, but with a W underlayer. (100) growth for this case was observed at annealing temperatures as low as 100 °C. Abnormal grain growth was suppressed for Cu deposited on Ta at 150 °C and annealed to 250 °C. A bimodal grain size distribution was observed in a similar sample deposited on W under identical conditions, however, the abnormally growing orientation for this case was observed to be the (111) orientation, not the (100) orientation.


2015 ◽  
Vol 87 ◽  
pp. 377-389 ◽  
Author(s):  
N. Sallez ◽  
X. Boulnat ◽  
A. Borbély ◽  
J.L. Béchade ◽  
D. Fabrègue ◽  
...  

Author(s):  
B. L. Soloff ◽  
T. A. Rado

Mycobacteriophage R1 was originally isolated from a lysogenic culture of M. butyricum. The virus was propagated on a leucine-requiring derivative of M. smegmatis, 607 leu−, isolated by nitrosoguanidine mutagenesis of typestrain ATCC 607. Growth was accomplished in a minimal medium containing glycerol and glucose as carbon source and enriched by the addition of 80 μg/ ml L-leucine. Bacteria in early logarithmic growth phase were infected with virus at a multiplicity of 5, and incubated with aeration for 8 hours. The partially lysed suspension was diluted 1:10 in growth medium and incubated for a further 8 hours. This permitted stationary phase cells to re-enter logarithmic growth and resulted in complete lysis of the culture.


Author(s):  
A.R. Pelton ◽  
A.F. Marshall ◽  
Y.S. Lee

Amorphous materials are of current interest due to their desirable mechanical, electrical and magnetic properties. Furthermore, crystallizing amorphous alloys provides an avenue for discerning sequential and competitive phases thus allowing access to otherwise inaccessible crystalline structures. Previous studies have shown the benefits of using AEM to determine crystal structures and compositions of partially crystallized alloys. The present paper will discuss the AEM characterization of crystallized Cu-Ti and Ni-Ti amorphous films.Cu60Ti40: The amorphous alloy Cu60Ti40, when continuously heated, forms a simple intermediate, macrocrystalline phase which then transforms to the ordered, equilibrium Cu3Ti2 phase. However, contrary to what one would expect from kinetic considerations, isothermal annealing below the isochronal crystallization temperature results in direct nucleation and growth of Cu3Ti2 from the amorphous matrix.


Author(s):  
B. H. Kear ◽  
J. M. Oblak

A nickel-base superalloy is essentially a Ni/Cr solid solution hardened by additions of Al (Ti, Nb, etc.) to precipitate a coherent, ordered phase. In most commercial alloy systems, e.g. B-1900, IN-100 and Mar-M200, the stable precipitate is Ni3 (Al,Ti) γ′, with an LI2structure. In A lloy 901 the normal precipitate is metastable Nis Ti3 γ′ ; the stable phase is a hexagonal Do2 4 structure. In Alloy 718 the strengthening precipitate is metastable γ″, which has a body-centered tetragonal D022 structure.Precipitate MorphologyIn most systems the ordered γ′ phase forms by a continuous precipitation re-action, which gives rise to a uniform intragranular dispersion of precipitate particles. For zero γ/γ′ misfit, the γ′ precipitates assume a spheroidal.


Author(s):  
R. E. Herfert

Studies of the nature of a surface, either metallic or nonmetallic, in the past, have been limited to the instrumentation available for these measurements. In the past, optical microscopy, replica transmission electron microscopy, electron or X-ray diffraction and optical or X-ray spectroscopy have provided the means of surface characterization. Actually, some of these techniques are not purely surface; the depth of penetration may be a few thousands of an inch. Within the last five years, instrumentation has been made available which now makes it practical for use to study the outer few 100A of layers and characterize it completely from a chemical, physical, and crystallographic standpoint. The scanning electron microscope (SEM) provides a means of viewing the surface of a material in situ to magnifications as high as 250,000X.


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