Photoactive G-Quadruplex Ligand Identifies Multiple G-Quadruplex-Related Proteins with Extensive Sequence Tolerance in the Cellular Environment

2021 ◽  
Vol 143 (4) ◽  
pp. 1917-1923
Author(s):  
Haomiao Su ◽  
Jinglei Xu ◽  
Yuqi Chen ◽  
Qi Wang ◽  
Ziang Lu ◽  
...  
Keyword(s):  
Extensive Sequence ◽  
Cellular Environment ◽  
G Quadruplex ◽  
Related Proteins

scholarly journals The Functional Role of Loops and Flanking Sequences of G-Quadruplex Aptamer to the Hemagglutinin of Influenza a Virus

2021 ◽  
Vol 22 (5) ◽  
pp. 2409
Author(s):  
Anastasia A. Bizyaeva ◽  
Dmitry A. Bunin ◽  
Valeria L. Moiseenko ◽  
Alexandra S. Gambaryan ◽  
Sonja Balk ◽  
...  
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Core Structure ◽  
Dna Aptamer ◽  
Tertiary Structures ◽  
Quadruplex Dna ◽  
G Quadruplex ◽  
Flanking Sequences ◽  
Related Proteins

Nucleic acid aptamers are generally accepted as promising elements for the specific and high-affinity binding of various biomolecules. It has been shown for a number of aptamers that the complexes with several related proteins may possess a similar affinity. An outstanding example is the G-quadruplex DNA aptamer RHA0385, which binds to the hemagglutinins of various influenza A virus strains. These hemagglutinins have homologous tertiary structures but moderate-to-low amino acid sequence identities. Here, the experiment was inverted, targeting the same protein using a set of related, parallel G-quadruplexes. The 5′- and 3′-flanking sequences of RHA0385 were truncated to yield parallel G-quadruplex with three propeller loops that were 7, 1, and 1 nucleotides in length. Next, a set of minimal, parallel G-quadruplexes with three single-nucleotide loops was tested. These G-quadruplexes were characterized both structurally and functionally. All parallel G-quadruplexes had affinities for both recombinant hemagglutinin and influenza virions. In summary, the parallel G-quadruplex represents a minimal core structure with functional activity that binds influenza A hemagglutinin. The flanking sequences and loops represent additional features that can be used to modulate the affinity. Thus, the RHA0385–hemagglutinin complex serves as an excellent example of the hypothesis of a core structure that is decorated with additional recognizing elements capable of improving the binding properties of the aptamer.

scholarly journals Sodium Lignosulfonate Improves Shoot Growth of Oryza Sativa via Enhancement of Photosynthetic Activity and Reduction of Stress Response

2020 ◽  
Author(s):  
Andrew De Xian Kok ◽  
Ngai Paing Tan ◽  
Wan Muhamad Asrul Nizam Wan Abdullah ◽  
Chu Nie Tang ◽  
Lee Yoon Low ◽  
...  
Keyword(s):  
Plant Growth ◽  
Shoot Growth ◽  
Growth Enhancement ◽  
Sodium Lignosulfonate ◽  
Cellular Environment ◽  
Lyase Activity ◽  
Growth Promoting ◽  
Phenylalanine Ammonia Lyase Activity ◽  
Biochemical Analyses ◽  
Related Proteins

Abstract Lignosulfonate (LS) is a by-product obtained during sulfite pulping process and is commonly used as a growth enhancer in plant growth. However, the underlying growth promoting mechanism of LS on shoot growth remains largely unknown. Hence, this study was undertaken to determine the potential application of eco-friendly LS chelated ion complex (NaLS and CaLS) to enhance recalcitrant indica rice MR219 shoot growth and to elucidate its underlying growth promoting mechanisms. The NaLS was shown to be a better shoot growth enhancer as compared to CaLS, with optimum concentration of 300 mg/L. Subsequent comparative proteomic analysis revealed an increase of photosynthesis-related proteins and stress regulator proteins abundance in NaLS-treated rice as compared to MSO (control). Consistently, biochemical analyses showed a significant increase of rubisco activity, total chlorophyll, total sugar and total protein contents in NaLS-treated rice, implying NaLS role in empowering photosynthesis activities that led to plant growth enhancement. In addition, low level of peroxidase activity, malondialdehyde content and phenylalanine ammonia lyase activity were also observed in NaLS-treated rice. These results suggest that NaLS plays a role in modulating cellular homeostasis to provide a conducive cellular environment for plant growth. Taken together, NaLS improved shoot growth of recalcitrant MR219 rice by upregulation of photosynthetic activities and reduction of cellular stress leading to better plant growth.

scholarly journals A Proteomics Analysis to Evaluate Cytotoxicity in NRK-52E Cells Caused by Unmodified Nano-Fe3O4

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
Yi-Reng Lin ◽  
Chao-Jen Kuo ◽  
Hugo You-Hsien Lin ◽  
Chin-Jen Wu ◽  
Shih-Shin Liang
Keyword(s):  
Cell Death ◽  
Cell Lines ◽  
Rat Kidney ◽  
Antagonistic Effect ◽  
Chaperone Proteins ◽  
Protective Effects ◽  
Proteomics Analysis ◽  
Cellular Environment ◽  
Nano Fe3o4 ◽  
Related Proteins

We synthesized unmodified Fe3O4nanoparticles (NPs) with particles size from 10 nm to 100 nm. We cultured NRK-52E cell lines (rat, kidney) and treated with Fe3O4NPs to investigate and evaluate the cytotoxicity of NPs for NRK-52E cells. Through global proteomics analysis using dimethyl labeling techniques and liquid phase chromatography coupled with a tandem mass spectrometer (LC-MS/MS), we characterized 435 proteins including the programmed cell death related proteins, ras-related proteins, glutathione related proteins, and the chaperone proteins such as heat shock proteins, serpin H1, protein disulfide-isomerase A4, endoplasmin, and endoplasmic reticulum resident proteins. From the statistical data of identified proteins, we believed that NPs treatment causes cell death and promotes expression of ras-related proteins. In order to avoid apoptosis, NRK-52E cell lines induce a series of protective effects such as glutathione related proteins to reduce reactive oxygen species (ROS), and chaperone proteins to recycle damaged proteins. We suggested that, in the indigenous cellular environment, Fe3O4NPs treatment induced an antagonistic effect for cell lines to go to which avoids apoptosis.

DNA-fragmentation and expression of apoptosis-related proteins in experimentally denervated and reinnervated rat facial muscle

1997 ◽  
Vol 23 (3) ◽  
pp. 141-149 ◽  
Author(s):  
D. S. Tews ◽  
H. H. Goebel ◽  
I. Schneider ◽  
A. Gunkel ◽  
E. Stennert ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Facial Muscle ◽  
Related Proteins

Characterization of embryogenesis-related proteins in alfalfa (Medicago sativa)

1996 ◽  
Vol 96 (4) ◽  
pp. 585-592 ◽  
Author(s):  
Randal W. Giroux ◽  
K. Peter Pauls
Keyword(s):  
Medicago Sativa ◽  
Related Proteins

Influence of AUF1 targeting siRNA on cell cycle-related proteins in thyroid cancer cell lines

2006 ◽  
Vol 114 (S 1) ◽  
Author(s):  
B Trojanowicz ◽  
Z Chen ◽  
J Bialek ◽  
Y Radestock ◽  
S Hombach-Klonisch ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Thyroid Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Thyroid Cancer Cell ◽  
Thyroid Cancer Cell Lines ◽  
Related Proteins

Decreased expression of genes encoding thermogenesis-related proteins in adipose tissues of obese patients is not associated with their methylation status

2014 ◽  
Author(s):  
Alina Kurylowicz ◽  
Marta Jonas ◽  
Wojciech Lisik ◽  
Maurycy Jonas ◽  
Zofia Agnieszka Wicik ◽  
...  
Keyword(s):  
Methylation Status ◽  
Obese Patients ◽  
Adipose Tissues ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Related Proteins

386-P: Acute Hypoglycemia Does Not Alter Serum Levels of Amyloid-Related Proteins Associated with Dementia

Diabetes ◽  
2020 ◽  
Vol 69 (Supplement 1) ◽  
pp. 386-P
Author(s):  
ABU MOIN ◽  
THOZHUKAT SATHYAPALAN ◽  
STEPHEN ATKIN ◽  
ALEXANDRA E. BUTLER
Keyword(s):  
Serum Levels ◽  
Acute Hypoglycemia ◽  
Related Proteins

1108 Proteomic analysis reveals increased abundance of inflammation-related proteins in adipose tissues from postpartum dairy cows treated with sodium salicylate

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 531-531
Author(s):  
M. Zachut ◽  
S. R. Montgomery ◽  
Y. Levin ◽  
L. Mamedova ◽  
B. J. Bradford
Keyword(s):  
Dairy Cows ◽  
Proteomic Analysis ◽  
Sodium Salicylate ◽  
Adipose Tissues ◽  
Related Proteins
Sign in / Sign up

Export Citation Format

Share Document