Decreased expression of genes encoding thermogenesis-related proteins in adipose tissues of obese patients is not associated with their methylation status

2014 ◽  
Author(s):  
Alina Kurylowicz ◽  
Marta Jonas ◽  
Wojciech Lisik ◽  
Maurycy Jonas ◽  
Zofia Agnieszka Wicik ◽  
...  
Keyword(s):  
Methylation Status ◽  
Obese Patients ◽  
Adipose Tissues ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Related Proteins

scholarly journals GABA Accumulation Causes Cell Elongation Defects and a Decrease in Expression of Genes Encoding Secreted and Cell Wall-Related Proteins in Arabidopsis thaliana

2011 ◽  
Vol 52 (5) ◽  
pp. 894-908 ◽  
Author(s):  
Hugues Renault ◽  
Abdelhak El Amrani ◽  
Ravishankar Palanivelu ◽  
Emily P. Updegraff ◽  
Agnès Yu ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Cell Wall ◽  
Cell Elongation ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Gaba Accumulation ◽  
Related Proteins

scholarly journals Coordination of plastid and nuclear gene expression

2003 ◽  
Vol 358 (1429) ◽  
pp. 135-145 ◽  
Author(s):  
John C. Gray ◽  
James A. Sullivan ◽  
Jun-Hui Wang ◽  
Cheryl A. Jerome ◽  
Daniel MacLean
Keyword(s):  
Nuclear Gene ◽  
Nuclear Genes ◽  
Regulation Of Transcription ◽  
Nuclear Gene Expression ◽  
Plastid Genomes ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Coordinated Expression ◽  
Plastid Protein ◽  
Related Proteins

The coordinated expression of genes distributed between the nuclear and plastid genomes is essential for the assembly of functional chloroplasts. Although the nucleus has a pre–eminent role in controlling chloroplast biogenesis, there is considerable evidence that the expression of nuclear genes encoding photosynthesis–related proteins is regulated by signals from plastids. Perturbation of several plastid–located processes, by inhibitors or in mutants, leads to decreased transcription of a set of nuclear photosynthesis–related genes. Characterization of arabidopsis gun ( genomes uncoupled ) mutants, which express nuclear genes in the presence of norflurazon or lincomycin, has provided evidence for two separate signalling pathways, one involving tetrapyrrole biosynthesis intermediates and the other requiring plastid protein synthesis. In addition, perturbation of photosynthetic electron transfer produces at least two different redox signals, as part of the acclimation to altered light conditions. The recognition of multiple plastid signals requires a reconsideration of the mechanisms of regulation of transcription of nuclear genes encoding photosynthesis–related proteins.

Expression of genes encoding PR10 class pathogenesis-related proteins is inhibited in yellow lupine root nodules

Plant Science ◽  
1999 ◽  
Vol 149 (2) ◽  
pp. 125-137 ◽  
Author(s):  
Michał M Sikorski ◽  
Jacek Biesiadka ◽  
Alina E Kasperska ◽  
Joanna Kopcińska ◽  
Barbara Łotocka ◽  
...  
Keyword(s):  
Root Nodules ◽  
Pathogenesis Related Proteins ◽  
Expression Of Genes ◽  
Pathogenesis Related ◽  
Genes Encoding ◽  
Yellow Lupine ◽  
Related Proteins

scholarly journals Insulin receptor substrate 1 gene expression is strongly up-regulated by HSPB8 silencing in U87 glioma cells

2020 ◽  
Vol 54 (4) ◽  
pp. 231-243
Author(s):  
Oksana S. Hnatiuk ◽  
Dariia O. Tsymbal ◽  
Dmytro O. Minchenko ◽  
Olena O. Khita ◽  
Yulia M. Viletska ◽  
...  
Keyword(s):  
Insulin Receptor ◽  
Quantitative Polymerase Chain Reaction ◽  
Glioma Cells ◽  
Insulin Receptor Substrate ◽  
Gene Expressions ◽  
Insulin Receptor Substrate 1 ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
The Stability ◽  
Related Proteins

Abstract Objective. The aim of the present investigation was to study the expression of genes encoding IRS1 (insulin receptor substrate 1) and some other functionally active proteins in U87 glioma cells under silencing of polyfunctional chaperone HSPB8 for evaluation of the possible significance of this protein in intergenic interactions. Methods. Silencing of HSPB8 mRNA was introduced by HSPB8 specific siRNA. The expression level of HSPB8, IRS1, HK2, GLO1, HOMER3, MYL9, NAMPT, PER2, PERP, GADD45A, and DEK genes was studied in U87 glioma cells by quantitative polymerase chain reaction. Results. It was shown that silencing of HSPB8 mRNA by specific to HSPB8 siRNA led to a strong down-regulation of this mRNA and significant modification of the expression of IRS1 and many other genes in glioma cells: strong up-regulated of HOMER3, GLO1, and PERP and down-regulated of MYL9, NAMPT, PER2, GADD45A, and DEK gene expressions. At the same time, no significant changes were detected in the expression of HK2 gene in glioma cells treated by siRNA, specific to HSPB8. Moreover, the silencing of HSPB8 mRNA enhanced the glioma cells proliferation rate. Conclusions. Results of this investigation demonstrated that silencing of HSPB8 mRNA affected the expression of IRS1 gene as well as many other genes encoding tumor growth related proteins. It is possible that the dysregulation of most of the studied genes in glioma cells after silencing of HSPB8 is reflected by a complex of intergenic interactions and that this polyfunctional chaperone is an important factor for the stability of genome function and regulatory mechanisms contributing to the tumorigenesis control.

scholarly journals Vitamin D Receptor Gene Expression in Adipose Tissue of Obese Individuals is Regulated by miRNA and Correlates with the Pro-Inflammatory Cytokine Level

2019 ◽  
Vol 20 (21) ◽  
pp. 5272 ◽  
Author(s):  
Marta Izabela Jonas ◽  
Alina Kuryłowicz ◽  
Zbigniew Bartoszewicz ◽  
Wojciech Lisik ◽  
Maurycy Jonas ◽  
...  
Keyword(s):  
Vitamin D ◽  
Adipose Tissue ◽  
Receptor Gene ◽  
Normal Weight ◽  
Mrna Levels ◽  
Adipose Tissues ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Obese Subjects ◽  
Inflammatory Milieu

Background: Given the role that vitamin D (VD) plays in the regulation of the inflammatory activity of adipocytes, we aimed to assess whether obesity changes the expression of VD-related genes in adipose tissue and, if so, to investigate whether this phenomenon depends on microRNA interference and how it may influence the local inflammatory milieu. Methods: The expression of genes encoding VD 1α-hydroxylase (CYP27B1), 24-hydroxylase (CYP24A1) and receptor (VDR), selected interleukins and microRNAs was evaluated by real-time PCR in visceral (VAT) and in subcutaneous (SAT) adipose tissues of 55 obese (BMI > 40 kg/m2) and 31 normal-weight (BMI 20–24.9 kg/m2) individuals. Results: VDR mRNA levels were higher, while CYP27B1 levels were lower in adipose tissues of obese patients than in those of normal-weight controls (VAT: P = 0.04, SAT: P < 0.0001 and VAT: P = 0.004, SAT: P = 0.016, respectively). The expression of VDR in VAT of obese subjects correlated negatively with levels of miR-125a-5p (P = 0.0006, rs = −0.525), miR-125b-5p (P = 0.001, rs = −0.495), and miR-214-3p (P = 0.009, rs = −0.379). Additionally, VDR mRNA concentrations in visceral adipose tissues of obese subjects correlated positively with mRNA levels of interleukins: 1β, 6 and 8. Conclusions: We observed obesity-associated up-regulation of VDR and down-regulation of CYP27B mRNA levels in adipose tissue. VDR expression correlates with the expression of pro-inflammatory cytokines and may be regulated by miRNAs.

scholarly journals Genes Encoding ACE2, TMPRSS2 and Related Proteins Mediating SARS-CoV-2 Viral Entry are Upregulated with Age in Human Cardiomyocytes

2020 ◽  
Author(s):  
Emma L. Robinson ◽  
Kanar Alkass ◽  
Olaf Bergmann ◽  
Janet J. Maguire ◽  
H. Llewelyn Roderick ◽  
...  
Keyword(s):  
Viral Entry ◽  
Enzyme Inhibitors ◽  
Cardiovascular Complications ◽  
Left Ventricular ◽  
Older Individuals ◽  
Human Cardiomyocytes ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Approved Drugs ◽  
Related Proteins

AbstractAge is an independent risk factor for adverse outcome in patients following COVID-19 infection. We hypothesised that differential expression of genes encoding proteins proposed to be required for entry of SARS-Cov-2 in aged compared to younger cardiomyocytes might contribute to the susceptibility of older individuals to COVID-19-associated cardiovascular complications.We generated strand-specific RNA-sequencing libraries from RNA isolated from flow-sorted cardiomyocyte nuclei from left ventricular tissue. RNASeq data were compared between five young (19-25yr) and five older (63-78yr) Caucasian males who had not been on medication or exhibited evidence of cardiovascular disease post-mortem.Expression of relevant genes encoding ACE2, TMPRSS2, TMPRS11D, TMPRS11E, FURIN, CTSL, CTSB and B0AT1/SLC6A19 were upregulated in aged cardiomyocytes and the combined relative cardiomyocyte expression of these genes correlated positively with age. Genes encoding proteins in the RAAS and interferon/interleukin pathways were also upregulated such as ACE, AGTR1, MAS1 and IL6R.Our results highlight SARS-CoV-2 related genes that have higher expression in aged compared with young adult cardiomyocytes. These data may inform studies using selective enzyme inhibitors/antagonists, available as experimental compounds or clinically approved drugs e.g. remdesivir that has recently been rapidly accepted for compassionate use, to further understand the contribution of these pathways in human cardiomyocytes to disease outcome in COVID-19 patients.

scholarly journals Glucose deprivation affects the expression of genes encoding cAMP-activated protein kinase and related proteins in U87 glioma cells in ERN1 dependent manner

2020 ◽  
Vol 54 (4) ◽  
pp. 244-254
Author(s):  
Oksana O. Ratushna
Keyword(s):  
Endoplasmic Reticulum ◽  
Protein Kinase ◽  
Endoplasmic Reticulum Stress ◽  
Glioma Cells ◽  
Glucose Deprivation ◽  
Expression Level ◽  
Gene Expressions ◽  
Expression Of Genes ◽  
Genes Encoding ◽  
Related Proteins

Abstract Objective. The aim of this investigation was to study the expression of genes encoding cAMP-activated protein kinase catalytic and regulatory A subunits (PRKACA and PRKAR1A) and related proteins such as cAMP-dependent protein kinase inhibitors A and G (PKIA and PKIG), catalytic subunit A of protein phosphatase 3 (PPP3CA), A-kinase anchoring protein 12 (AKAP12), and praja ring finger ubiquitin ligase 2 (PJA2) in U87 glioma cells in response to glucose deprivation in both control U87 glioma cells and cells with ERN1 (endoplasmic reticulum to nucleus signaling 1) knockdown, the major pathway of the endoplasmic reticulum stress signaling, for evaluation of possible significance of glucose deprivation in ERN1 dependent regulation of glioma growth. Methods. The expression level of PRKA related genes was studied in control (transfected by vector) and ERN1 knockdown U87 glioma cells under glucose deprivation by real-time quantitative polymerase chain reaction. Results. It was shown that the expression level of PRKACA and PKIA genes was down-regulated in control glioma cells treated by glucose deprivation, but PJA2 gene was up-regulated. At the same time, the expression of four other genes (PRKAR1A, PKIG, AKAP12, and PPP3CA) was resistant to this experimental condition. Furthermore, ERN1 knockdown of glioma cells significantly modified the effect glucose deprivation on the expression almost all studied genes. Thus, treatment of glioma cells with inhibited ERN1 enzymatic activity by glucose deprivation lead to a more significant down-regulation of the expression level of PKIA and to suppression PRKAR1A gene expressions. Moreover, the ERN1 knockdown introduced up-regulation of PKIG and AKAP12 gene expressions in glioma cells treated by glucose deprivation and eliminated the sensitivity of PJA2 gene to this experimental condition. Conclusions. Results of this investigation demonstrated that ERN1 knockdown significantly modified the sensitivity of most studied PRKA related gene expressions to glucose deprivation and that these changes are a result of complex interactions of variable endoplasmic reticulum stress related and unrelated regulatory factors and contributed to the suppression of glioma cell proliferation and their possibly chemoresistance.

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