A New Test Method for the Evaluation of Total Antioxidant Activity of Herbal Products

2004 ◽  
Vol 52 (1) ◽  
pp. 21-25 ◽  
Author(s):  
Olga A. Zaporozhets ◽  
Olena A. Krushynska ◽  
Natalia A. Lipkovska ◽  
Valentina N. Barvinchenko
2007 ◽  
Vol 33 (3) ◽  
pp. 375-376 ◽  
Author(s):  
M. F. Borisenkov ◽  
L. A. Erunova ◽  
E. M. Lyuseva ◽  
N. V. Pozdeeva

2019 ◽  
Vol 59 (7) ◽  
pp. 134-141
Author(s):  
Anatoly A. Lapin ◽  
◽  
Irek G. Garifullin ◽  
Valery N. Zelenkov ◽  
◽  
...  

The article presents the results of biochemical studies of the total antioxidant activity of the hepatoprotective collection of plant origin and its individual components, which show that it consists of birch leaves (Betulae folia), tansy flowers (Tanaceti flores), phytohepatol No. 2 (choleretic collection No. 2) and individual its components have pronounced antioxidant activity. More antioxidant activity founded in tansy and true hepatoprotective collection. Comparative tests of the flowers of meadowsweet, with the highest antioxidant activity, showed the promise of their use in the collections of medicinal plants as effective natural metabolic regulators. The total antioxidant activity of the water extract collection at 13.56% rel. exceeded the calculated value obtained by summing the activities of the individual components of the collection, which indicates the presence of synergism in their action, which may be of value for use in the treatment and prevention of liver diseases. When all components of the drug collection are finished, the indicator increases by 11-24 % rel., which indicates the thermal stability of the antioxidant substances that make up their composition. In the oxidation of aqueous extracts of the components of hepatoprotective collection with hydrogen peroxide, the most stable were antioxidants of birch leaves. It was found that collection only at a ratio of 7.7 g per liter of water shows synergism (15 % rel.), and the rest of all the samples we tested are antagonistic. The detected increase in the antioxidant activity of the new collection compared with the effects of its individual components allows us to consider it as a promising dosage form for use in the treatment and prevention of liver diseases.


2020 ◽  
Vol 7 (4) ◽  
Author(s):  
Bince Mani ◽  
Sinjumol Thomas

Antioxidant compounds play a significant role in preventing and scavenging free-radicals by reducing oxidative stress and providing protection to humans against degenerative diseases and infections. Obviously, antioxidant molecules of plant origin are pivotal to combat the oxidative harm in cells. Present work intended to evaluate the antioxidant capacity of the fruits of Spondias pinnata. Methanol extracts of the fruits at two stages of maturity were prepared and investigated by various antioxidants analyses such as total antioxidant activity, reducing power and radical scavenging assays (DPPH, nitric oxide and hydrogen peroxide). Its total phenol, flavonoid and tannin contents were also determined. Spondias pinnata fruit extracts exhibited effective antioxidant activity and its IC50 values of the unripe fruits were 65, 66, 72.23, 83.25, 66.75 ?g/ml and ripe fruits were 124.24, 92.50, 97.66, 144.10, 72.25 ?g/ml, for total antioxidant activity, reducing power, DPPH radical scavenging, nitric oxide radical scavenging and hydrogen peroxide scavenging assays respectively. The extracts, especially unripe fruit extract had good amounts of total phenolic and flavonoid contents which might contribute the antioxidant activities considerably. It is evident from the study that the fruits of S. pinnata possessed potent antioxidant activity and it can be considered as a good dietary choice among the underutilized fruits as well as common fruits. Therefore, wild mango may be a good choice of antioxidants of plant origin for dietary and pharmaceutical uses.


2006 ◽  
Vol 54 (19) ◽  
pp. 7355-7363 ◽  
Author(s):  
Kanjana Mahattanatawee ◽  
John A. Manthey ◽  
Gary Luzio ◽  
Stephen T. Talcott ◽  
Kevin Goodner ◽  
...  

2019 ◽  
Vol 46 (No. 2) ◽  
pp. 90-97
Author(s):  
Bożena Pawłowska ◽  
Jarmila Neugebauerova ◽  
Monika Bieniasz ◽  
Bożena Szewczyk-Taranek ◽  
Ivo Ondrasek

Stability of Rosa agrestis, R. canina, R. dumalis and R. rubiginosa regenerants after shoot tip cryopreservation was monitored with the use of biochemical markers and pollen quality assessment in matured plants cultivated in the field. The results of rosa pollen and hip evaluation revealed no significant differences between post-cryopreserved regenerants and control plants; however, variations in the assessed markers were noted between Rosa species. Analyses of hips confirmed high levels of vitamin C in these plants; the highest was observed in R. dumalis (750–870 mg/100 g fresh weight). High total antioxidant activity (TAC) was as follows: DPPH – 50–250 mM TE/100 dry weight, FRAP – 80–180 mM TE/100 g dry weight. The present study showed a high content of flavonoids (TFC) and phenols (TPC). Post-cryopreserved plants retained pollen specific for Caninae: numerous anthers, abundant pollen, poor pollen germination and viability and typical pollen morphology. This study demonstrates new tools for evaluating the fidelity of post-cryopreserved regenerants.


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