scholarly journals Systematic Definition of Protein Constituents along the Major Polarization Axis Reveals an Adaptive Reuse of the Polarization Machinery in Pheromone-Treated Budding Yeast

2009 ◽  
Vol 8 (1) ◽  
pp. 6-19 ◽  
Author(s):  
Rammohan Narayanaswamy ◽  
Emily K. Moradi ◽  
Wei Niu ◽  
G. Traver Hart ◽  
Matthew Davis ◽  
...  
2020 ◽  
Vol 12 (24) ◽  
pp. 10561
Author(s):  
Maria Cerreta ◽  
Alessia Elefante ◽  
Ludovica La Rocca

The international debate on the adaptive reuse of cultural heritage sites consistent with the Sustainable Development Goals has become increasingly important in the implementation of circular economy models for urban policies. The new values that characterize cultural assets, considered the result of a collaborative process, can enhance both manufactured and human capital, and provide the basis for a system of relationships that binds them. Furthermore, the values of historical artistic assets produced by community-based regeneration processes are particularly relevant when they characterize abandoned commons and cult buildings, to which communities attribute an identity and symbolic value. Starting from the definition of the concept of complex social value, we propose a methodological process that combines approaches and techniques typical of deliberative evaluations and collaborative decision-making processes. The aim is to identify the complex value chains generated by adaptive reuse, in which intrinsic values can play a driving role in the regeneration strategies of discarded cultural heritage. The experimentation, tested with the project “San Sebastiano del Monte dei Morti Living Lab” (SSMOLL), activates a creative and cultural Living Lab in the former Morticelli church, in the historic center of Salerno, in southern Italy. The reuse project is part of a more comprehensive process of social innovation and culture-led urban regeneration triggered in Salerno starting from SSMOLL. The partial results of the process show how a co-exploration phase has characterized the cultural characteristic of the living lab and how the co-evaluation of the individual activities orient the possible reuse scenarios. Finally, the results provide a first analysis of the relationship types activated.


Buildings ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 105 ◽  
Author(s):  
Ekin Eray ◽  
Benjamin Sanchez ◽  
Carl Haas

Adaptive reuse of buildings is considered a superior alternative for new construction in terms of sustainability and a disruptive practice in the current capital project delivery model for the renewal of today’s built environment. In comparison to green-field construction projects, adaptive reuse projects require distinct stages, definition of interfaces, decision gates, and planning methods in order to secure the success of the building project. Unfortunately, little research has been done regarding establishing feasible systems for the planning, assessment, and management of adaptive reuse projects, leading to underperforming building projects outcomes. Interface management (IM) can improve renovation projects outcomes by defining appropriate ways to identify, record, monitor, and track project interfaces. IM has the potential of bringing cost and time benefits during adaptive reuse projects execution. The aim of this study is to develop a reference framework for implementing IM for adaptive reuse projects. First, the inefficiencies of redevelopment projects are explained inside of a circular economy (CE) context. Second, an ontology of IM for adaptive reuse projects is defined based on the current barriers to adaptive reuse and the most common interface problems in construction projects. Third, the defined ontology is expanded through a case study by showing examples of adaptive reuse barriers on a case project, and how IM could have been part of the solution for these problems. Finally, this study concludes with the suggestions on interface management systems (IMS) implementation for future adaptive reuse projects.


1966 ◽  
Vol 24 ◽  
pp. 3-5
Author(s):  
W. W. Morgan

1. The definition of “normal” stars in spectral classification changes with time; at the time of the publication of theYerkes Spectral Atlasthe term “normal” was applied to stars whose spectra could be fitted smoothly into a two-dimensional array. Thus, at that time, weak-lined spectra (RR Lyrae and HD 140283) would have been considered peculiar. At the present time we would tend to classify such spectra as “normal”—in a more complicated classification scheme which would have a parameter varying with metallic-line intensity within a specific spectral subdivision.


1975 ◽  
Vol 26 ◽  
pp. 21-26

An ideal definition of a reference coordinate system should meet the following general requirements:1. It should be as conceptually simple as possible, so its philosophy is well understood by the users.2. It should imply as few physical assumptions as possible. Wherever they are necessary, such assumptions should be of a very general character and, in particular, they should not be dependent upon astronomical and geophysical detailed theories.3. It should suggest a materialization that is dynamically stable and is accessible to observations with the required accuracy.


1979 ◽  
Vol 46 ◽  
pp. 125-149 ◽  
Author(s):  
David A. Allen

No paper of this nature should begin without a definition of symbiotic stars. It was Paul Merrill who, borrowing on his botanical background, coined the termsymbioticto describe apparently single stellar systems which combine the TiO absorption of M giants (temperature regime ≲ 3500 K) with He II emission (temperature regime ≳ 100,000 K). He and Milton Humason had in 1932 first drawn attention to three such stars: AX Per, CI Cyg and RW Hya. At the conclusion of the Mount Wilson Ha emission survey nearly a dozen had been identified, and Z And had become their type star. The numbers slowly grew, as much because the definition widened to include lower-excitation specimens as because new examples of the original type were found. In 1970 Wackerling listed 30; this was the last compendium of symbiotic stars published.


Author(s):  
K. T. Tokuyasu

During the past investigations of immunoferritin localization of intracellular antigens in ultrathin frozen sections, we found that the degree of negative staining required to delineate u1trastructural details was often too dense for the recognition of ferritin particles. The quality of positive staining of ultrathin frozen sections, on the other hand, has generally been far inferior to that attainable in conventional plastic embedded sections, particularly in the definition of membranes. As we discussed before, a main cause of this difficulty seemed to be the vulnerability of frozen sections to the damaging effects of air-water surface tension at the time of drying of the sections.Indeed, we found that the quality of positive staining is greatly improved when positively stained frozen sections are protected against the effects of surface tension by embedding them in thin layers of mechanically stable materials at the time of drying (unpublished).


Author(s):  
W. A. Shannon ◽  
M. A. Matlib

Numerous studies have dealt with the cytochemical localization of cytochrome oxidase via cytochrome c. More recent studies have dealt with indicating initial foci of this reaction by altering incubation pH (1) or postosmication procedure (2,3). The following study is an attempt to locate such foci by altering membrane permeability. It is thought that such alterations within the limits of maintaining morphological integrity of the membranes will ease the entry of exogenous substrates resulting in a much quicker oxidation and subsequently a more precise definition of the oxidative reaction.The diaminobenzidine (DAB) method of Seligman et al. (4) was used. Minced pieces of rat liver were incubated for 1 hr following toluene treatment (5,6). Experimental variations consisted of incubating fixed or unfixed tissues treated with toluene and unfixed tissues treated with toluene and subsequently fixed.


Author(s):  
J. D. Hutchison

When the transmission electron microscope was commercially introduced a few years ago, it was heralded as one of the most significant aids to medical research of the century. It continues to occupy that niche; however, the scanning electron microscope is gaining rapidly in relative importance as it fills the gap between conventional optical microscopy and transmission electron microscopy.IBM Boulder is conducting three major programs in cooperation with the Colorado School of Medicine. These are the study of the mechanism of failure of the prosthetic heart valve, the study of the ultrastructure of lung tissue, and the definition of the function of the cilia of the ventricular ependyma of the brain.


Author(s):  
P. M. Lowrie ◽  
W. S. Tyler

The importance of examining stained 1 to 2μ plastic sections by light microscopy has long been recognized, both for increased definition of many histologic features and for selection of specimen samples to be used in ultrastructural studies. Selection of specimens with specific orien ation relative to anatomical structures becomes of critical importance in ultrastructural investigations of organs such as the lung. The uantity of blocks necessary to locate special areas of interest by random sampling is large, however, and the method is lacking in precision. Several methods have been described for selection of specific areas for electron microscopy using light microscopic evaluation of paraffin, epoxy-infiltrated, or epoxy-embedded large blocks from which thick sections were cut. Selected areas from these thick sections were subsequently removed and re-embedded or attached to blank precasted blocks and resectioned for transmission electron microscopy (TEM).


Author(s):  
K.P.D. Lagerlof

Although most materials contain more than one phase, and thus are multiphase materials, the definition of composite materials is commonly used to describe those materials containing more than one phase deliberately added to obtain certain desired physical properties. Composite materials are often classified according to their application, i.e. structural composites and electronic composites, but may also be classified according to the type of compounds making up the composite, i.e. metal/ceramic, ceramic/ceramie and metal/semiconductor composites. For structural composites it is also common to refer to the type of structural reinforcement; whisker-reinforced, fiber-reinforced, or particulate reinforced composites [1-4].For all types of composite materials, it is of fundamental importance to understand the relationship between the microstructure and the observed physical properties, and it is therefore vital to properly characterize the microstructure. The interfaces separating the different phases comprising the composite are of particular interest to understand. In structural composites the interface is often the weakest part, where fracture will nucleate, and in electronic composites structural defects at or near the interface will affect the critical electronic properties.


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